• Journal of Periodontal and Implant Science
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• 제48권2호
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• pp.84-91
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• 2018

Purpose: The purpose of this study was to visualize and identify peri-implant bone defects in optical coherence tomography (OCT) images and to obtain quantitative measurements of the defect depth. Methods: Dehiscence defects were intentionally formed in porcine mandibles and implants were simultaneously placed without flap elevation. Only the threads of the fixture could be seen at the bone defect site in the OCT images, so the depth of the peri-implant bone defect could be measured through the length of the visible threads. To analyze the reliability of the OCT measurements, the flaps were elevated and the depth of the dehiscence defects was measured with a digital caliper. Results: The average defect depth measured by a digital caliper was $4.88{\pm}1.28mm$, and the corresponding OCT measurement was $5.11{\pm}1.33mm$. Very thin bone areas that were sufficiently transparent in the coronal portion were penetrated by the optical beam in OCT imaging and regarded as bone loss. The intraclass correlation coefficient between the 2 methods was high, with a 95% confidence interval (CI) close to 1. In the Bland-Altman analysis, most measured values were within the threshold of the 95% CI, suggesting close agreement of the OCT measurements with the caliper measurements. Conclusions: OCT images can be used to visualize the peri-implant bone level and to identify bone defects. The potential of quantitative non-invasive measurements of the amount of bone loss was also confirmed.

• 디지털융복합연구
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• 제17권6호
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• pp.315-326
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• 2019

본 연구는 프로바이오틱스의 특성과 관련 연구에 대한 종설이다. 프로바이오틱스는 면역질환 개선 효과, 유당불내증 완화 효과, 변비 및 설사 개선 효과, 항암 및 항종양 효과, 심혈관계 질환 예방 효과, 항당뇨 효과, 항산화 효과, 항균효과, 유용 대사산물의 생산 등의 기능성을 입증하였다. 구강 프로바이오틱스는 젖산을 생성하여 충치의 원인이 되는 뮤탄스균과 잇몸 질환을 일으키는 진지발리스균을 비롯한 유해균이 서식하기 어려운 환경을 만들어 구강 생태계 조성과 구취 제거에 효과적이라는 연구가 입증되었다. 그 결과 질환 완화는 물론 건강한 구강 밸런스를 관리하는 예방법으로 주목받고 있다. 일부 제한점에도 불구하고 현재 유용한 임상연구가 진행되고 있으며, 본 연구는 치아우식증과 치주염, 칸디다의 집락화 등 구강상태를 개선하기 위한 프로바이오틱스의 근거가 될 수 있을 것이다.

• 대한통합의학회지
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• 제10권4호
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• pp.35-47
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• 2022

Purpose : As the number of patients with systemic diseases is increasing in the old, the relevance of oral health is gaining particular research interest. To provide fundamental resources for dental services, this study examined the relationship between doctors' diagnoses of circulatory diseases and patients' awareness of oral health and oral care behaviors. Methods : SPSS 26.0 was used to assess various variables, including doctor's diagnosis of circulatory diseases, gender, age, household income quintile, participation in economic activity, marital status, subjective level of oral health awareness, mastication discomfort, speaking and chewing discomfort, dental inspection, use of oral care goods, teeth brushing during the previous day, and untreated oral conditions. Results : The circulatory diseases suffered by the subjects were as follows: 56 % high blood pressure, 36 % dyslipidemia, 6 % stroke, and 8 % myocardial infarction or angina. A higher age meant a higher diagnosis rate of high blood pressure (p<.001) and stroke (p<.001). Those with dyslipidemia showed a higher rate of receiving oral inspection (p=.040), and an untreated oral condition was more frequently observed among those not diagnosed with the disease (p=.035). The subjects who were not diagnosed with stroke showed a higher rate of oral inspection (p<.001), while those who had a prior experience of stroke suffered a higher rate of mastication discomfort (p=.020). People who had high blood pressure showed a lower rate of using oral care goods (p<.001), and those diagnosed with stroke showed a lower rate of brushing teeth the previous day. Conclusion : This study found a correlation between the diagnosis of circulatory diseases and the awareness of oral health and oral care behavior. Consequently, oral health education should be included in mental health-related education, and customized training to teach teeth brushing and the use of oral care goods should be provided to patients with circulatory diseases during dentist visits.

• 대한통합의학회지
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• 제11권2호
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• pp.149-157
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• 2023

Purpose : In this study, to prove the antibacterial effect of Disocorea batatas, which is widely used for food, and to confirm the growth inhibitory effect, the antibacterial activity against L. gasseri, S. mutans, and P. gingivalis was verified. Based on this, it is intended to verify the utility as a preventive and therapeutic composition for dental caries and periodontal disease. Methods : RAW 264.7 cells were used to verify the cell survival rate and NO (Nitric Oxide) inhibitory effect on Disocorea batatas ethanol extract (DBEE). In order to verify the antibacterial effect against L. gasseri, S. mutans, and P. gingivalis, concentrations of 125, 250, and 500 ㎎/㎖ of DBEE were used and measured by the disk diffusion method. In order to confirm the growth inhibitory effect, the absorbance was measured at 600 ㎚ at 3, 6, 12, 18, and 24 hours using the liquid medium dilution method, and the growth inhibitory effect was measured compared to the control group. Results : The cell viability for DBEE was 91 % at 50 ㎎/㎖, and there was no cytotoxicity. The NO production inhibitory effect was shown from 10 ㎍/ml, and the higher the concentration, the greater the inhibitory effect. As for the antimicrobial effect using the disk diffusion method, the higher the concentration, the higher the antibacterial effect. At 125 ㎎/㎖ and 250 ㎎/㎖, S. mutans and L. gasseri showed high antimicrobial activity, and at 500 ㎎/㎖, the antibacterial effect was higher in L. gasseri. The growth inhibitory effect in DBEE was concentration-dependent as the higher the concentration, the higher the growth inhibitory effect, and all of them began to show growth inhibitory effects from 6 hours. Conclusion : Considering that it is widely used as an edible and medicinal material, Disocorea batatas has shown the potential to be used as a substance to prevent and alleviate dental caries and periodontal diseases, and it is believed that further research can be applied to oral health care products.

• Journal of Periodontal and Implant Science
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• 제42권3호
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• pp.88-94
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• 2012

Purpose: The use of appropriate instruments to clean surfaces with minimal change, is critical for the successful maintenance of a dental implant. However, there is no consensus about the type and methodology for such instruments. The aim of this study was to characterize changes in the roughness of titanium surfaces treated by various scaling instruments. Methods: Thirty-seven identical disks (5 mm in diameter) were investigated in this study. The specimens were divided into eight groups according to the types of instrumentation and the angle of application. Ultrasonic scaling systems were applied on a titanium disk to simulate standard clinical conditions. The equipment included a piezoelectric ultrasonic scaler with a newly developed metallic tip (NS group), a piezoelectric ultrasonic scaler with a conventional tip (CS group), a piezoelectric root planer ultrasonic scaler with a conventional tip (PR group), and a plastic hand curette (PH group). In addition, the sites treated using piezoelectric ultrasonic scaler systems were divided two sub-groups: 15 and 45 degrees. The treated titanium surfaces were observed by scanning electron microscopy (SEM), and the average surface roughness (Ra) and mean roughness profile depth (Rz) were measured with a profilometer. Results: SEM no significant changes in the titanium surfaces in the NS group, regardless of the angle of application. The PH group also showed no marked changes to the titanium surface, although some smoothening was observed. All CS and PR sites lost their original texture and showed irregular surfaces in SEM analysis. The profilometer analysis demonstrated that the roughness values (Ra and Rz) of the titanium surfaces increased in all, except the PH and NS groups, which showed roughness decreases relative to the untreated control group. The Ra value differed significantly between the NS and PR groups (P<0.05). Conclusions: The results of this study indicated that changes in or damage to titanium surfaces might be more affected by the hardness of the scaler tip than by the application method. Within the limitations of this study, the newly developed metallic scaler tip might be especially suitable for peri-implant surface decontamination, due to its limited effects on the titanium surface.

• 치위생과학회지
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• 제15권5호
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• pp.659-665
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• 2015

DCU의 물을 관리하는 다양한 방법들 중 치과용 유니트를 사용하기 전 수관 물 빼기 방법이 가장 사용이 쉽고 간단하기 때문에, 미국질병관리본부와 미국치과의사협회에서 환자 진료 전후에 20~30초 동안 물을 미리 배출시킬 것을 권장하고 있다. 하지만, 수관 물 빼기의 권장 시간인 20~30초와 관련한 과학적 근거가 명확하지 않고, 20~30초 이외의 시간 동안 수관 물 빼기 효과를 확인한 연구가 부족한 실정이다. 본 연구의 목적은 권장시간인 20~30초뿐만아니라 이보다 더 짧거나 긴 시간 동안 치과용 유니트 수관 물 빼기를 수행한 후 각 시간별 오염 세균 수의 감소 정도를 조사하여 20~30초 권장시간의 적절성을 확인하고 더 효과적인 수관 물 빼기 시간이 있는지를 조사하는 것이다. 물 시료 수집은 치과대학 학생 실습 시뮬레이션실 내 치과용 유니트를 대상으로 수행하였다. 7개 치과용 유니트의 초음파치석제거기에서 15분 동안 수관 물 빼기를 진행하면서 30초 간격으로 배출되는 물을 수집하였다. 추가적으로 5개의 치과용 유니트에서는 1분 동안 물을 배출시키면서 10초 간격으로 물을 수집하였다. 물 시료 내 오염 세균 수는 R2A agar 배지에 수집된 물을 도말한 후 배양하여 확인하였다. 7개의 치과용 유니트에서 최대 15분 동안 물을 배출시켰을 때 치과용 유니트의 오염 수준이 200 CFU/ml 이하로 감소되지 않았다. 추가적으로 5개의 치과용 유니트에서 1분 동안 10초 간격으로 배출되는 물을 수집하여 세균 수를 확인하였을 때 10초 동안 수관 물 빼기를 한 후 세균 수 감소율은 평균 63%였고, 20초 동안 수관 물 빼기를 한 후 세균 수 감소율은 평균 84%였다. 본 실험의 결론은 다음과 같다: 첫째, 수관 물빼기의 권장시간인 20~30초뿐만아니라 최대 15분 동안 수관 물 빼기를 시행하는 것은 권장수준(${\leq}200CFU/ml$)으로 세균 오염도를 감소시킬 수 없었다. 둘째, 수관 물 빼기의 권장 시간인 20~30초보다 짧게 10초 동안 수관 물 빼기를 하는 것도 세균 수 감소에 효과가 있었다. 셋째, 치과용 유니트 내 물의 세균 오염수준을 권장 수준으로 유지시키기 위해서는 수관 물 빼기와 함께 주기적으로 화학 소독제로 치과용 유니트 수관을 소독하는 것이 필요하다.

• Journal of Oral Medicine and Pain
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• 제34권4호
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• pp.363-369
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• 2009

미란형 구강편평태선과 재발성 아프타성 구내염은 T림프구에 의해 매개되는 염증성 면역질환이다. T림프구에 영향을 주는 다양한 조절 인자들 중 CD28, CD45, CD152, CD154, CD279등의 mRNA가 미란형 구강편평태선 및 재발성 아프타성 구내염 환자들의 비자극성 전타액내에서 어떻게 발현되는지를 살펴보고, 이것의 진단학적 가치를 평가하고자 한다. 미란형 구강편평태선으로 진단받은 환자군 18명, 재발성 아프타성 구내염으로 진단받은 환자군 12명, 정상군 8명에게서 비자극성 전타액을 10분간 채득한 후, 상피세포를 분리하여 mRNA를 추출하였다. Real time PCR을 이용하여 각 군의 T림프구 조절 인자들의 mRNA 발현 양상을 비교분석하였다. 미란형 구강편평태선의 T림프구 조절인자들의 mRNA 발현 양상은 정상인과 비교 결과, CD45, CD279는 높게 측정되었고, CD154는 낮게 측정되었다. 재발성 아프타성 구내염 환자들의 T림프구 조절인자들의 mRNA 발현 양상은 정상인과 비교 결과, CD45, CD279는 높게 발현되었고, CD28, CD154는 낮게 발현되었다. 부가적으로 미란형 구강편평태선 환자군의 타액내 CD152의 발현 양상은 재발성 아프타성 구내염 환자군보다 높게 발현되었다. 미란형 구강편평태선과 재발성 아프타성 구내염 환자들의 비자극성 전타액내 T림프구 조절인자들의 mRNA 발현 양상은 각 질환의 진단에 기여할 수 있을 것으로 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권4호
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• pp.380-385
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• 2008

양측성 구순구개열을 가진 16세 여자 환자가 소구증과 구순열비변형으로 인하여 개구 제한과 심미적 문제를 주소로 내원하였다. 병력에서 구순성형술, 구개성형술 그리고 이차구순비성형술을 받았으며, 임상 소견에서 상순과 하순이 매우 작고 입술의 폭은 40 mm 정도로 짧았으며 최대 개구량은 20 mm였다. Converse의 협점막 외전 피판을 이용한 양측성 구각성형술을 시행하였으며 수술 후 입술의 폭은 60 mm로 증가하였다. 수술 1년 경과 후 입술의 폭은 54mm로 약 6 mm 정도 회귀되었고, 그 외에 별다른 문제점 없이 심미적, 기능적으로 양호한 결과를 보였다. 협점막 외전 피판을 이용한 양측성 구각성형술은 입술의 폭과 크기를 증가시켜주고 입술의 외관을 개선하여 소구증의 외과적 교정에 적절한 술식임을 알 수 있었다.

• Journal of Periodontal and Implant Science
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• 제31권2호
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• pp.401-420
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• 2001

A novel glucanhydrolase from a mutant of Lipomyces starkeyi(KSM 22)has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependent adherent microbial film and Lipomyces starkeyi KSM 22 dextranase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi KSM 22 dextranase are desirable for its application as a dental plaque control agent. This study was performed to determine oral hygiene benefits and safety of dextranase(Lipomyces starkeyi KSM 22 dextranase)-containing mouthwash in human experimental gingivitis. This 3-week clinical trial was placebo-controlled double-blind design evaluating 1U/ml dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 2 and 3 weeks, subjects were scored for plaque(Silness and $L{\ddot{o}e$ plaque index and plaque severity index), gingivitis($L{\ddot{o}e$ and Silness gingival index), and at baseline and 3 weeks of experiment, subjects were scored for plaque(Turesky-Quingley-Hein's plaque index and plaque severity index), tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice dailywithout toothbrushing. All the groups showed significant increase in plaque accumulation since 1 week of experiment. During 3 weeks' period, the dextranase group showed the least increase in plaque accumulation of Silness and $L{\ddot{o}e$ plaque index, compared to the chlorhexidine and placebo groups, but chlorhexidine group showed the least increase inplaque accumulation of Turesky-Quingley-Hein's plaque index. As for gingival inflammation, all the groups showed significant increase during 3 weeks of experiment. The dextranase group also showed the least increase in gingival index score, compared to the chlorhexidine as well as the placebo groups. Whereas the tooth stain was increased significantly in the chlorhexidine group, compared to the baseline score and the placebo group since 3 weeks of mouthrinsing. It was significantly increased after 3 weeks in the dextranase group, still less severe than the chlorhexidine group. As for the oral side effect, the dextranase group showed less tongue accumulation, bad taste, compared to the chlorhexidine group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase was comparable to 0.12% chlorhexidine mouthwashin inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, in human experimental gingivitis. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.

• Journal of Periodontal and Implant Science
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• 제31권2호
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• pp.371-388
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• 2001

A novel glucanhydrolase(DXAMase) from a mutant of Lipomyces starkeyi(KSM 22) has been shown effective in hydrolysis of mutan, reduction of mutan formation by Streptococcus mutans and removal pre-formed sucrose-dependentadherent microbial film and DXAMase has been strongly bound to hydroxyapatitie. These in vitro properties of Lipomyces starkeyi DXAMase are desirable for its application as a dental plaque control agent. This study was performed to determine the adjunctive oral hygiene benefits and safety of dextranase(Lipomyces starkeyi KSM 22 DXAMase)-containing mouthwash when used alongside normal tooth-brushing. This 6-month clinical trial was placebo-controlled double-blind design evaluating 1U/ml dextranase mouthwash and 0.12% chlorhexidine mouthwash. A total 39 systemically healthy subjects, who had moderate levels of plaque and gingivitis were included. At baseline, 1, 3 and 6 months, subjects were scored for plaque accumulation(Turesky modification of Quingley-Hein's plaque index), gingivitis status($L\ddot{o}e$ and Silness gingival index), and tooth stain(Area and severity index system by Lang et al). Additionally, oral mucosal examinations were performed and subjects questioned for adverse symptoms. Two weeks after pre-experiment examinations and a professional prophylaxis, the subjects provided with allocated mousewash and instructed to use 20-ml volumes for 30s twice daily after toothbrushing. All the groups showed significant increase in plaque accumulation since 1 month of experiment. During 6 months' period, the Dextranase mouthwash group showed the least increase in plaque accumulation, compared to the Chlorhexidine mouthwash and placebo groups. As for gingival inflammation, all the groups showed significant increase during 6 months of experiment. The Experimental group(Dextranase mouthwash) also showed the least increase in gingival index score, compared to the Positive control(Chlorhexidine mouthwash)as well as the Negative control(placebo)groups. Whereas the tooth stain was increased significantly in the Positive control group, compared to the baseline score and the Negative controlgroup since 3 months of mouthrinsing. It was significantly increased after 6 months in the Experimental group, still less severe than the Positive control group. As for the oral side effect, the Experimental group showed less tongue accumulation, bad taste, compared to the Positive control group. From these results, mouthrinsing with Lipomyces starkeyi KSM 22 dextranase provided adjunctive benefits to toothbrushing, comparable to 0.12% chlorhexidine mouthwash in inhibition of plaque accumulation and gingival inflammation and local side effects were if anything less frequent and less intense than chlorhexidine, with long-term use of the mouthwash. All data had provided positive evidence for Lipomyces starkeyi KSM 22 dextranase as an antiplaque agent and suggested that further development of dextranase formulations for plaque control are warranted.