• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.339-344
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• 2004

A method for synthesizing branched fructo-oligosaccharides (BFOS) with a high concentration of sucrose ($1{\~}3$ M) was developed using levansucrase prepared from Leuconortoc mesenteroides B-1355C. The degree of polymerization of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The synthesized BFOS were stable at a pH ranges of 2 to 4 under $120^{\circ}C$. The percentage of BFOS in the reaction digest was $95.7\%$ (excluding monosaccharides; $4.3\%$ was levan). BFOS reduced the insoluble glucan formation by Streptococcus sobrinus on the surfaces of glass vials or stainless steel wires in the presence of sucrose. They also reduced the growth and acid productions of S, sobrinus. Oligosaccharides can be used as sweeteners for foods such as beverages requiring thermo- and acid-stable properties and 3s potential inhibitors of dental caries.

• 보건교육건강증진학회지
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• 제26권1호
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• pp.129-140
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• 2009

Objectives: This study aimed to examined the socioeconomic disparities in oral health related behaviors and to assess if those behaviors eliminate socioeconomic disparities in oral health in a nationally representative sample of adults aged 30-64. Methods: Data are from the Korea Third National Health and Nutrition Examination Survey (2005). Behaviors were indicated by smoking, over intake of daily calories from carbohydrate, perceived stress, frequency of daily tooth brushing, use of oral hygiene goods, insufficient oral treatment. Oral health outcomes were self-reported dental caries and periodontitis during the last 12 months and perceived oral health. Education, household income, and employed status indicated socioeconomic position. Sex, age, residential area, marital status were adjusted for in the logistic regression analysis. Logistic regression analysis was used to assess socioeconomic disparities in behaviors. Logistic regression model adjusting and not adjusting for behaviors were compared to assess the change in socioeconomic disparities in oral health. Results: Clear socioeconomic disparities in all behaviors were showed. After adjusting for behaviors, the association between oral health and socioeconomic indicators attenuated but did not disappear. For example, the odd ratios of reporting poorer oral health for persons in no education or elementary school education and middle school education groups, compared with college or higher education group, were 1.77 (95% CI: 1.36-2.29) and 1.56 (1.19-1.97), respectively. After adjusting for all indicators of behaviors, these odds ratios attenuated to 1.54 (1.17-2.03) and 1.48 (1.15-1.91) for those groups, respectively. Conclusion: These findings suggest that the presence of more complex determinants of socioeconomic disparities in oral health should be considered with developing preventive policies for those disparities.

• 대한치과보철학회지
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• 제50권3호
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• pp.204-209
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• 2012

치은연 하방에 치아 우식증이나 치아 파절이 발생했을 때, 치아를 탈구시켜 즉시 정출시키는 외과적 정출술을 이용한 치관 연장술은 자연치를 보존하고 추가적인 치주적 수술 없이 단기간에 심미적인 보철 치료를 가능하게 한다. 본 환자는 16세 남환으로 본원 보존과로부터 상악 좌측 중절치의 심미 수복을 위해 본원 보철과로 의뢰되었다. 상악 좌측 중절치는 근관치료가 되어 있었으며 치관-치근 파절로 인해 협측 치관 길이는 4mm였으며 구개측 판막을 열었을 때 근심 구개측 치경부 파절 범위는 치은 하방 3-4mm에 위치하였다. 외과적 정출술을 통한 치관연장술을 시행하였으며 경과 관찰 및 임시 치아의 단계를 거쳐 3개월 후에 전부 도재관으로 수복하여 만족할 만한 임상결과를 얻을 수 있었다.

• 대한소아치과학회지
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• 제36권3호
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• pp.475-480
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• 2009

저위교합이란 치아가 출은한 이후 유착 등의 원인으로 맹출이 정지되어 주위 인접치 보다 교합평면이 낮아지는 것을 말한다. 저위교합은 인접치들의 경사, 저위교합된 부위의 공간상실, 대합치의 정출, 해당부위 치아우식 감수성의 증가, 후속 영구치의 맹출경로 변위, 매복, 회전 등의 문제점을 유발할 수 있으므로 조기 진단 및 정기검진을 통한 적절한 치료가 필요하다. 저위교합된 유구치는 대부분 영구 계승치가 존재하며 일반적으로 정상 탈락한다. 그러나 저위교합된 유구치의 탈락이 지연되고 저위교합의 정도가 심화되어 해당부위의 공간상실이 발생하면, 공간부족으로 인하여 후속 영구치의 매복을 야기할 수 있다. 이러한 경우 교정적으로 공간확장술을 시행한 후 외과적 발치를 고려해야 한다. 본 증례는 하악 제 2유구치의 저위교합이 관찰된 3명의 환아를 대상으로 공간에 대한 평가 후 필요한 경우 공간확장술을 시행하고 발치를 시행함으로써 저위교합으로 인해 발생된 문제점을 개선하고, 추후에 발생될 합병증을 예방하였기에 이를 보고하는 바이다.

• 한국환경보건학회지
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• 제29권2호
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• pp.38-44
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• 2003

This study was performed to investigate the levels of fluoride (F), calcium (Ca), and magnesium (Mg) in water samples taken from five reservoirs or direct sources on a stream used for agricultural or domestic water in the Iksan and Wanjoo areas, Jeon-buk, Korea, and to find a possible link between Ca or Mg and fluoride in water sources in theses areas. The samples were collected by the recommendation methods of the World Health Organization and analyzed by the recommendations of the Japanese Standard Methods. Statistical analyses were performed by the analysis of variance and correlation analysis. The F levels in water samples wire 0.80~2.53 ppm. In four sampling sites the water fluoride levels exceeded 1 ppm. which if the recommended level for fluorosis/caries control. The Ca levels in water samples were 6.82~12.98 ppm, and the Mg levels were 0.30~1.97 ppm, which are lower compared with the natural levels of water sources previously reported by other investigators. This study showed a positive correlation between Ca and Mg (r= 0.8779. p<0.01) and a negative correlation between F and Ca (r=-0.6974, p<0.05) and also between F and Mg (r=-0.5581) in the water samples. However, the study did not find remarkable relationships in fluoride levels between sampling sites. These results support the fact that there were epidemics of dental fluorosis in this area. The lack of significant positive correlations in fluoride levels between sampling sites suggests that there may be some pathways for the transfer of the metal to the water through other environmental media besides the water course. Long-term epidemiological studies are needed on the relationship between high F together with low Ca and Mg levels in the water, and total human health in this community. There should alto be a long-term monitoring of the water quality in this area.

• Childhood Kidney Diseases
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• 제11권1호
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• pp.100-105
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• 2007

Cockayne 증후군은 지능저하와 발육부전을 특징으로 하며 피부의 광과민성, 색소성의 망막증, 백내장, 신경전도성 귀머거리 외에 다기관 이상을 동반하는 드문 질환이다. 신장학적 이상 소견 또한 드물지 않게 동반되는 합병증 중 하나이며 병리학적으로 사구체의 유리질화, 세뇨관의 위축 및 간질 내 섬유화가 관찰될 수 있다. 저자들은 7세된 남아에서 Cockayne 증후군의 특징적인 외모와 임상양상을 확인하고 동반된 국소 분절성 사구체 경화증을 진단하였기에 문헌 고찰과 함께 보고하는 바이다.

• 대한미생물학회지
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• 제35권1호
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• pp.77-85
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• 2000

Streptococcus mutans is the most important causative bacteria of dental caries among the oral bacteria. Lactococcus lactis 1370 was isolated from the oral cavity of child. The effect of Lactococcus lactis 1370 on the formation of artificial plaque by Streptococcus mutans was studied. 1. The insoluble substances and bacteria were much more attached on the wall of disposable cuvette in the culture of Streptococcus mutans than in the combined culture of Streptococcus mutans and Lactococcus lactis 1370. 2. The mean weight of produced artificial plaque on the wires in the beaker was 131.7 mg in the culture of Streptococcus mutans only, whereas being reduced to 6.4 mg in the combined culture of Streptococcus mutans and Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 3. When Streptococcus mutans was cultured in the media containing culture supernatant of Lactococcus lactis 1370 cultured in M17 broth containing 0.5% yeast extract and 5% sucrose, the mean weight of produced artificial plaque was 8.0 mg on the wires, whereas being 125.4 mg in the media without culture supernatant of Lactococcus lactis 1370 (p<0.05). The viable cell didn't show the significant difference between them after culturing. 4. When Streptococcus mutans was cultured in the media containing soluble polymer produced by Lactococcus lactis 1370, the mean weight of produced artificial plaque was significantly reduced compared with being cultured in the media without soluble polymer (p<0.05). The viable cell didn't show the significant difference between them after culturing. 5. The soluble polymer produced by Lactococcus lactis 1370 was glucan. 6. The glucan produced by Lactococcus lactis 1370 was water-soluble glucan containing ${\alpha}$-1,6-glucose linkage as the main linkage. These results suggest that the artificial plaque formed by Streptococcus mutans is inhibited by water-soluble glucan produced by Lactococcus lactis 1370.

• 한국미생물·생명공학회지
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• 제28권5호
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• pp.270-278
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• 2000

The study was performed to investgate the excellent microbial anticaries substance which is more effective that the chlorhexidine in the dental caries treatment. A typi-cal strain which produced the most excellent antimicrobial subatance was selected. and identified novel alkalophillic Bacillus alkalophilshaggy JY-827. For the maximal production of themicrobial antibiotic against Streptococcus mutans from B. alkalophilshaggy JY-827, the optimal culture condition was in the medium containing glucose 15g/ L, pepton 10g/L and $K_2$$HPO_4$ 2g/L the highest production of antibiotic against S.mutans was obtained at $25^{\circ}C$ and pH 11.0 for 5 days. The antibiotic from B. alkalophilshaggy JY-827 was purified by organic solvent extraction, silica gel and sephadex LH-20 column chromatograpies, and then crystallized with methanol. The crystallin compoma-tion of this antibiotic was as a curcular shape. The melting point and rm[$\alpha$]$D^{20}$ were 152-154$^{\circ}C$ and +55。, respec-tively. Based on Instumental analyses such as FT-IR, $^{1}$H-NMR $^{13}$ C-NMR and GC-mass, the antibiotic was identified as aminoglycoside. It was obtained as amorphous white power, and soluble in water power, and soluble in water, methanol but insoluble in ether, chroloform. This antibiotic inhibited the growth of S.mutans to about 3 day at the concentration of $2.5$\times$10^{-7}$ /M. It was stable at the alkalli condition but unstable within the acid condition. It was also stable up to $70^{\circ}C$.

• International Journal of Oral Biology
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• 제39권4호
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• pp.201-206
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• 2014

This study investigated the antimicrobial activity of methanol extract of mulberry leaf against 16 strains of mutans streptococci and four species of periodontopathogens: Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extracts or silica gel chromatography fractions of methanol-extracted mulberry leaf were evaluated by determining minimal inhibitory concentrations using an established microdilution method. The cytotoxicity of the extracts of mulberry leaf on KB cells was tested by the methyl thiazolyl tetrazolium assay. Chromatography fraction 12 displayed the most potent antimicrobial activity against all 16 strains of mutans streptococci, P. gingivalis, and P. intermedia. No KB cell cytotoxicity was evident up to $128{\mu}g/ml$ of fraction 12. The methanol extract had no antimicrobial activity against F. nucleatum and A. actinomycetemcomitans. These results suggest chromatography fraction 12 methanol extract of mulberry leaf could be useful in the development of oral hygiene products, such as dentifrice and oral hygiene solution, for the prevention of dental caries.

• International Journal of Oral Biology
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• 제39권4호
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• pp.193-199
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• 2014

Fluoride has been accepted as an important material for oral health and is widely used to prevent dental caries in dentistry. However, its safety is still questioned by some. Autophagy has been implicated in cancer cell survival and death, and may play an important role in oral cancer. This study was undertaken to examine whether sodium fluoride (NaF) modulates autophagy in SCC25 human tongue squamous cell carcinoma cells. NaF demonstrated anticancer activity via autophagic and apoptotic cell death. Autophagic vacuoles were detectable using observed to form by monodansylcadaverine (MDC) and acridine orange (AO). Analysis of NaF-treated SCC25 cells for the presence of biochemical markers revealed direct effects on the conversion of LC-3II, degradation of p62/SQSTM1, cleavage formation of ATG5 and Beclin-1, and caspase activation. NaF-induced cell death was suppressed by the autophagy inhibitor 3-methyladenine (3-MA). NaF-induced autophagy was confirmed as a pro-death signal in SCC25 cells. These results implicate NaF as a novel anticancer compound for oral cancer therapy.