Browse > Article
http://dx.doi.org/10.11620/IJOB.2014.39.4.193

NaF-induced Autophagy on SCC25 Human Tongue Squamous Cell Carcinoma Cells  

Kang, Jin-Mo (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Lee, Bo-Young (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Kim, In-Ryoung (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Kim, Yong-Ho (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Yu, Su-Bin (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Park, Hae-Ryoun (Department of Oral Pathology, School of Dentistry, Pusan National University)
Park, Bong-Soo (Department of Oral Anatomy, School of Dentistry, Pusan National University)
Publication Information
International Journal of Oral Biology / v.39, no.4, 2014 , pp. 193-199 More about this Journal
Abstract
Fluoride has been accepted as an important material for oral health and is widely used to prevent dental caries in dentistry. However, its safety is still questioned by some. Autophagy has been implicated in cancer cell survival and death, and may play an important role in oral cancer. This study was undertaken to examine whether sodium fluoride (NaF) modulates autophagy in SCC25 human tongue squamous cell carcinoma cells. NaF demonstrated anticancer activity via autophagic and apoptotic cell death. Autophagic vacuoles were detectable using observed to form by monodansylcadaverine (MDC) and acridine orange (AO). Analysis of NaF-treated SCC25 cells for the presence of biochemical markers revealed direct effects on the conversion of LC-3II, degradation of p62/SQSTM1, cleavage formation of ATG5 and Beclin-1, and caspase activation. NaF-induced cell death was suppressed by the autophagy inhibitor 3-methyladenine (3-MA). NaF-induced autophagy was confirmed as a pro-death signal in SCC25 cells. These results implicate NaF as a novel anticancer compound for oral cancer therapy.
Keywords
NaF; autophagy; apoptosis; human squamous cell carcinoma;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ozsvath D. Fluoride and environmental health: a review. Rev Environ Sci Biotechnol. 2009;8:59-79.   DOI
2 Li Y. Fluoride: safety issues. J Indiana Dent Assoc. 1993;72:22-26.
3 Lee JH, Jung JY, Jeong YJ, Park JH, Yang KH, Choi NK, Kim SH, Kim WJ. Involvement of both mitochondrial- and death receptor-dependent apoptotic pathways regulated by Bcl-2 family in sodium fluoride-induced apoptosis of the human gingival fibroblasts. Toxicology. 2008;243:340-347.   DOI   ScienceOn
4 Codogno P. Autophagy in cell survival and death. J Soc Biol. 2005;199:233-241.   DOI
5 Zhang JQ, Li YM, Chen XH, Liu T, Chen YT, He WT, Zhang QB, Liu SY. Autophagy is involved in anticancer effects of matrine on SGC-7901 human gastric cancer cells. Oncol Rep. 2011;26:115-124.
6 Michaud M, Martins I, Sukkurwala AQ, Adjemian S, Ma Y, Pellegatti P, Shen S, Kepp O, Scoazec M, Mignot G, Rello-Varona S, Tailler M, Menger L, Vacchelli E, Galluzzi L, Ghiringhelli F, di Virgilio F, Zitvogel L, Kroemer G. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science 2011;334:1573-1577.   DOI
7 Giannopoulou E, Antonacopoulou A, Matsouka P, Kalofonos HP. Autophagy: Novel Action of Panitumumab in Colon Cancer. Anticancer Res. 2009;29:5077-5082.
8 Gozuacik D, Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004;23:2891-2906.   DOI   ScienceOn
9 Guo XL, Li D, Hu F, Song JR, Zhang SS, Deng WJ, Sun K, Zhao QD, Xie XQ, Song YJ, Wu MC, Wei LX. Targeting autophagy potentiates chemotherapy-induced apoptosis and proliferation inhibition in hepatocarcinoma cells. Cancer Lett. 2012;320:171-179.   DOI
10 Clayman GL, Ebihara S, Terada M, Mukai K, Goepfert H. Report of the Tenth International Symposium of the Foundation for Promotion of Cancer Research: Basic and clinical research in head and neck cancer. Jpn J Clin Oncol. 1997;27:361-368.   DOI
11 Suzuki M, Endo M, Shinohara F, Echigo S, Rikiishi H. Enhancement of cisplatin cytotoxicity by SAHA involves endoplasmic reticulum stress-mediated apoptosis in oral squamous cell carcinoma cells. Cancer Chemother Pharmacol. 2009;64:1115-1122.   DOI
12 Bell RB, Kademani D, Homer L, Dierks EJ, Potter BE. Tongue cancer: Is there a difference in survival compared with other subsites in the oral cavity? J Oral Maxil Surg. 2007;65:229-236.   DOI   ScienceOn
13 Lo WL, Kao SY, Chi LY, Wong YK, Chang RCS. Outcomes of oral squamous cell carcinoma in Taiwan after surgical therapy: Factors affecting survival. J Oral Maxil Surg. 2003;61:751-758.   DOI   ScienceOn
14 Shintani S, Li CN, Mihara M, Klosek SK, Terakado N, Hino S, Hamakawa H. Anti-tumor effect of radiation response by combined treatment with angiogenesis inhibitor, TNP-470, in oral squamous cell carcinoma. Oral Oncol. 2006;42:66-72.   DOI
15 Song JS, Lee HY, Lee E, Hwang HJ, Kim JH. Cytotoxicity and apoptosis induction of sodium fluoride in human promyelocytic leukemia (HL-60) cells. Environ Toxicol Phar. 2002;11:85-91.   DOI   ScienceOn
16 Anuradha CD, Kanno S, Hirano S. Fluoride induces apoptosis by caspase-3 activation in human leukemia HL-60 cells. Arch Toxicol. 2000;74:226-230.   DOI   ScienceOn
17 Anuradha CD, Kanno S, Hirano S. Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL-60 cells. Free Radical Bio Med. 2001;31:367-373.   DOI   ScienceOn
18 Tsujimoto Y, Shimizu S. Another way to die: autophagic programmed cell death. Cell Death Differ 2005;12:1528-1534.   DOI
19 Otsuki S, Morshed S, Chowdhury S, Takayama F, Satoh T, Hashimoto K, Sugiyama K, Amano O, Yasui T, Yokote Y. Possible link between glycolysis and apoptosis induced by sodium fluoride. J Dent Res. 2005;84:919-923.   DOI
20 Suzuki M, Bartlett JD. Sirtuin1 and autophagy protect cells from fluoride-induced cell stress. Biochim Biophys Acta. 2014;1842:245-255.   DOI
21 Levine B, Yuan J. Autophagy in cell death: an innocent convict? J Clin Invest. 2005;115:2679-2688.   DOI   ScienceOn
22 Chen LH, Loong CC, Su TL, Lee YJ, Chu PM, Tsai ML, Tsai PH, Tu PH, Chi CW, Lee HC, Chiou SH. Autophagy inhibition enhances apoptosis triggered by BO-1051, an N-mustard derivative, and involves the ATM signaling pathway. Biochem Pharmacol. 2011;81:594-605.   DOI
23 Ichimura Y, Komatsu M. Selective degradation of p62 by autophagy. Semin Immunopathol. 2010;32:431-436.   DOI
24 Ichimura Y, Kominami E, Tanaka K, Komatsu M. Selective turnover of p62/A170/SQSTM1 by autophagy. Autophagy 2008;4:1063-1066.   DOI
25 Pankiv S, Clausen TH, Lamark T, Brech A, Bruun JA, Outzen H, Overvatn A, Bjorkoy G, Johansen T. p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem. 2007;282:24131-24145.   DOI   ScienceOn
26 Fimia GM, Stoykova A, Romagnoli A, Giunta L, Di Bartolomeo S, Nardacci R, Corazzari M, Fuoco C, Ucar A, Schwartz P, Gruss P, Piacentini M, Chowdhury K, Cecconi F. Ambra1 regulates autophagy and development of the nervous system. Nature 2007;447:1121-1125.
27 Kihara A, Kabeya Y, Ohsumi Y, Yoshimori T. Beclinphosphatidylinositol 3-kinase complex functions at the trans-Golgi network. Embo Rep. 2001;2:330-335.   DOI   ScienceOn
28 Cho DH, Jo YK, Hwang JJ, Lee YM, Roh SA, Kim JC. Caspase-mediated cleavage of ATG6/Beclin-1 links apoptosis to autophagy in HeLa cells. Cancer Lett. 2009;274:95-100.   DOI   ScienceOn
29 Sun QM, Fan WL, Zhong Q. Regulation of Beclin 1 in autophagy. Autophagy 2009;5:713-716.   DOI   ScienceOn
30 Wirawan E, Vande Walle L, Kersse K, Cornelis S, Claerhout S, Vanoverberghe I, Roelandt R, De Rycke R, Verspurten J, Declercq W, Agostinis P, Vanden Berghe T, Lippens S, Vandenabeele P. Caspase-mediated cleavage of Beclin-1 inactivates Beclin-1-induced autophagy and enhances apoptosis by promoting the release of proapoptotic factors from mitochondria. Cell Death Dis. 2010;1:e18.   DOI
31 Djavaheri-Mergny M, Maiuri MC, Kroemer G. Cross talk between apoptosis and autophagy by caspase-mediated cleavage of Beclin 1 Oncogene 2010;29:1717-1719.   DOI   ScienceOn
32 Betin VMS, Lane JD. Caspase cleavage of Atg4D stimulates GABARAP-L1 processing and triggers mitochondrial targeting and apoptosis. J Cell Sci. 2009;122:2554-2566.   DOI
33 Jounai N, Takeshita F, Kobiyama K, Sawano A, Miyawaki A, Xin KQ, Ishii KJ, Kawai T, Akira S, Suzuki K, Okuda K. The Atg5 Atg12 conjugate associates with innate antiviral immune responses. Proc Natl Acad Sci USA. 2007;104:14050-14055.   DOI   ScienceOn
34 Lum JJ, Bauer DE, Kong M, Harris MH, Li C, Lindsten T, Thompson CB. Growth factor regulation of autophagy and cell survival in the absence of apoptosis. Cell 2005;120:237-248.   DOI   ScienceOn
35 Petersen PE, Lennon MA. Effective use of fluorides for the prevention of dental caries in the 21st century: the WHO approach. Community Dent Oral Epidemiol. 2004;32:319-21.   DOI   ScienceOn
36 Yorimitsu T, Klionsky DJ. Autophagy: molecular machinery for self-eating. Cell Death Differ. 2005;12 (Suppl 2):1542-1552.   DOI   ScienceOn
37 Shen J, Huang CH, Jiang L, Gao F, Wang Z, Zhang YY, Bai JP, Zhou HM, Chen QM. Enhancement of cisplatin induced apoptosis by suberoylanilide hydroxamic acid in human oral squamous cell carcinoma cell lines. Biochem Pharmacol. 2007;73:1901-1909.   DOI   ScienceOn
38 Hansen KJ, Clemen LA, Ellefson ME, Johnson HO. Compound-specific, quantitative characterization of organic fluorochemicals in biological matrices. Environ Sci Technol. 2001;35:766-770.   DOI   ScienceOn