• Journal of the Korean Institute of Gas
• /
• v.17 no.1
• /
• pp.26-34
• /
• 2013

The multiphase flow analysis related to phase change can be adapted to lots of areas such as evaporation and condensation has many interesting branches due to complicated phenomenon. In this study, the experimental investigation of cryogenic liquid in the porous media with various densities was shown how the cryogenic liquid behaves in the porous structure. For this study, permeability behaviors under different applying pressure of the glass wool with different bulk densities are discussed. Experimental investigation on the behavior of cryogenic liquefied nitrogen in the porous media is conducted. The result was that the non linearity of pressure gradient with location is increased and the permeability is decreased as the bulk density of glass wool increased. Lastly, simulation results with CFD commercial package program are used to realize the cryogenic liquid's flow in porous media to compare the finding with experimental results.

• Journal of Advanced Marine Engineering and Technology
• /
• v.35 no.4
• /
• pp.414-420
• /
• 2011

Thermal stress analysis of a planar anode-supported SOFC considering electrochemical reactions has been performed under operating conditions where average current density varies from 0 to 2000 $A/m^2$. For the case of the 2000 $A/m^2$ operating condition, Structural stress analysis based on the temperature distributions obtained from the CFD analysis of the unit cell has also been done. From this one way Fluid-Structure Interaction(FSI) analysis, Maximum Von-Mises stress under negligible temperature gradient fields occurs when cell components are perfectly bonded. The maximum stress of the electrolyte, cathode and anode in a unit cell SOFC is 262.58MPa, 28.55MPa and 15.1MPa respectively. The maximum thermal stress is critically dependent on static friction coefficient.

• Journal of Pharmaceutical Investigation
• /
• v.31 no.3
• /
• pp.185-190
• /
• 2001

Human papillomavirus (HPV) infection is known to cause cervical cancers. Human papillomavirus-like particles (VLP) have been studied as preventive vaccines of cervical cancers. To develop VLP as a therapeutic gene carrier, we studied the method to encapsulate cytokine genes in virus-like particles. HPV type 16 capsid L1 genes were amplified by polymerase chain reaction and cloned into T vector. L1 gene was then inserted into baculovirus transfer vector. The clone of baculovirus encoding L1 gene was isolated and used to express L1 protein in Sf 21 insect cells. VLP were purified by CsCl density gradient and ultracentrifugation. VLP were disassembled to capsomer units by treatment of a reducing agent. Given that interleukin-2 (IL-2) genes have been used in anticancer gene therapy and as a molecular adjuvant, IL-2 cytokine plasmids were chosen as a model gene. IL-2 plasmids were incubated with the disassembled capsomer suspension. To reassemble the particles, the mixture of capsomers and cytokine plasmids was dialyzed. The disassembly and reassembly of VLP were confirmed by transmission electron microscopy. The entrapment of cytokine plasmids in reassembled VLP was tested by the stability of plasmids against DNase I. After treatment of reassembled virus-like particles with DNase I, discrete IL-2 DNA band was observed. Our results indicate that IL-2 cytokine plasmid (3.5 kb size) can be encapsulated in the virus-like particles, suggesting the potential of VLP as a gene delivery system. Moreover, VLP containing the adjuvant cytokine plasmids might function as more effective subunit vaccines.

• Journal of Astronomy and Space Sciences
• /
• v.19 no.1
• /
• pp.7-18
• /
• 2002

Interstellar cabon monoxide and its isotopes were observed with the 13.7m radio telescope of the Daeduk Radio Astronomy Observatory toward Orion Molecular Cloud-1 (OMC-1). We derive the excitation temperature, optical depth, column density, and isotopic abundance ratios from the observed lines of $^{12}CO,^{13}CO,\;and\;C^{18}O$ inside of the region of $11'{\times}11'$. The optical depths of $^{13}CO$ were obtained to be 0.1~0.4, while those of $C^{18}O$ to be 0.01~0.03. The isotopic ratios $^{12}C/^{13}C$ were also estimated to be 2~60. We could not find the radial isotopic ratio $^{12}C/^{13}C$ gradient in the OMC-1.

• Journal of Korea Multimedia Society
• /
• v.15 no.11
• /
• pp.1369-1376
• /
• 2012

In this paper, we propose 2D industrial image registration method for the detection of defects. Proposed method performs preprocessing to smooth the original image with the preservation of the edge for the robust registration against general noise. Then, x-direction gradient magnitude image and corresponding binary image are generated. Density analysis around neighborhood regions per pixel are performed to generate feature image for preventing mis-registration due to moire-like patterns, which frequently happen in industrial images. Finally, 2D image registration based on phase correlation between feature images is performed to calculate translational parameters to align two images rapidly and optimally. Experimental results showed that the registration accuracy of proposed method for the real industrial images was 100% and our method was about twenty times faster than the previous method. Our fast and accurate method could be used for the real industrial applications.

• Proceedings of the Polymer Society of Korea Conference
• /
• 2006.10a
• /
• pp.333-333
• /
• 2006

We synthesized two novel polynorbornene derivatives, chiral poly(norbornene acid methyl ester) (C-PNME) and racemic poly(norbornene acid n-butyl ester) (R-PNME), which are potential low dielectric constant materials for applications in advanced microelectronic and display devices. Thin films of these polymers deposited on substrates were investigated by structural analyses using synchrotron grazing incidence X-ray scattering, specular reflectivity and ellipsometry. These analyses provided important information on the structure, electron density gradient across film thickness, chain orientation, refractive index and thermal expansion of the polymers in substrate-supported thin films. The structural characteristics and properties of the thin films were first dependent on the polymer chain' tacticity and further influenced by film thickness and thermal annealing.

• Korean Journal of Microbiology
• /
• v.39 no.2
• /
• pp.118-122
• /
• 2003

To develop vector plasmid for the bacteriophage P2-P4 system which is a useful experimental tool for the study of viral capsid assembly, we constructed a new P4-derived vector plasmid starting from P4 ash8 sid71 With recombinant DNA technology, a portion of P4 genome was deleted and tetracyclin resistance gene (terR) was introduced into P4 genome to give P4 selectivity. Resulting P4 ash8(sid71) terR was 12.09 kb long and could be converted to a viable bacteriophage with P2 infection. The burst size of induced bacteriophage form of P4 ash8(sid71) terR was determined. The CsCl buoyant equilibrium density gradient experiment of new P4 derivative suggested the upper limit of packaging capacity in P2-size head.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.1
• /
• pp.309-314
• /
• 2013

Aim: Brain tumors almost universally have fatal outcomes; new therapeutics are desperately needed and will only come from improved understandins of glioma biology. Methods: Exosomes are endosomally derived 30~100 nm membranous vesicles released from many cell types. Examples from GL26 cells were here purified using density gradient ultracentrifugation and monitored for effects on GL26 tumor growth in C57BL/6j mice (H-2b). Lactate dehydrogenase release assays were used to detect the cytotoxic activity of CD8+T and NK cells. Percentages of immune cells producing intracellular cytokines were analyzed by FACS. Results: In this study, exosomes from murine-derived GL26 cells significantly promoted in vivo tumor growth in GL26-bearing B6 mice. Then we further analyzed the effects of the GL26 cells-derived exosomes on immune cells including CD8+T, CD4+T and NK cells. Inhibition of CD8+T cell cytotoxic activity was demonstrated by CD8+T cell depletion assays in vivo and LDH release assays in vitro. The treatment of mice with exosomes also led to a reduction in the percentages of CD8+T cells in splenocytes as determined by FACS analysis. Key features of CD8+T cell activity were inhibited, including release of IFN-gamma and granzyme B. There were no effects of exosomes on CD4+T cells and NK cells. Conclusion: Based on our data, for the first time we demonstrated that exosomes from murine derived GL26 cells promote the tumor growth by inhibition of CD8+T cells in vivo and thus may be a potential therapeutic target.

• Clinical and Experimental Reproductive Medicine
• /
• v.46 no.4
• /
• pp.147-151
• /
• 2019

Objective: The aim of this study was to investigate DNA fragmentation status in human spermatozoa according to specific tail swelling patterns determined via hypo-osmotic swelling test (HOST). Methods: Frozen semen samples from 21 healthy donors were thawed and prepared by the swim-up technique for use in intracytoplasmic sperm injection. The semen samples were treated for 5 minutes as part of the HOST procedure and then underwent the sperm chromatin dispersion test using a Halosperm kit. DNA fragmentation status (large halo, medium halo, small halo, no halo, or degraded) and the specific tail swelling pattern ("a"-"g") were assessed at the level of a single spermatozoon. A total of 42,000 spermatozoa were analyzed, and the percentage of spermatozoa without DNA fragmentation (as evidenced by a large or medium halo) was assessed according to the specific tail swelling patterns observed. Results: The HOST examinations showed that > 93% of spermatozoa across all types displayed no DNA fragmentation. The percentage of spermatozoa without DNA fragmentation was 100% in type "d", 98.67% in type "g", and 98.17% in type "f" spermatozoa. Conclusion: We found that the type "d" spermatozoa displayed no DNA fragmentation, but the other types of spermatozoa also displayed very low rates of DNA fragmentation. This result may be associated with the processing of the spermatozoa by density gradient centrifugation and the swim-up technique.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.05a
• /
• pp.622-624
• /
• 2015

Tumor hypoxia caused by the unique characteristics of solid tumor sites such as lowered vascular density, irregular vasculature, longitudinal oxygen gradient, and unbalanced oxygen consumption has decreased therapeutic efficacy in several clinical trials such as radiation, chemotherapy, and surgery. Hence, tumor oxygenation studies at microvascular levels are important to provide better understanding of the complexity of microvasculature oxygen transport and exchange with tissue. However, polarographic microelectodes, was employed to measure $pO_2$ at the microvasculature level, but it is difficult to perform and does not provide significant spatial and temporal information of oxygen delivery. In this research, we introduce the hyperspectral imaging system able to provide a wide range of vascular characteristics by spatial maps on hemoglobin saturation information for better understanding of the relationship between blood oxygen delivery, hypervascularity, aberrant angiogenesis at microvasculature levels during tumor growth.