• 제목/요약/키워드: densitometry

검색결과 123건 처리시간 0.027초

Penicillium islandicum에 의한 황변미독(黃變米毒) Luteoskyrin의 생성(生成) (Formation of Luteoskyrin by Penicillium islandicum)

  • 김용화;이서래
    • 한국식품과학회지
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    • 제10권1호
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    • pp.46-51
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    • 1978
  • 황변미독(黃變米毒)의 일종인 luteoskyrin의 동정(同定) 및 정량법(定量法)을 검토한 후 변질미에서 분리된 Pen. islandicum의 luteoskyrin 생성능(生成能)을 추구한 결과는 다음과 같다. 1) Luteoskyrin은 0.5 N oxalic acid impregnated silicagel G plate로서 acetone : n-hexane : water(6:3:1.5, upper layer)를 전개용매로하는 TLC방법에서 분리(分離)가 가장 좋았다. 분리된 황색물질의 최대흡수파장(吸收波長)은 426, 448nm이었으며 chromatogram을 태양광선에 $2{\sim}3$시간 노출시 자색(紫色)으로 변하였다. 2) Luteoskyrin의 정량법으로서 TLC전개(展開)후 용출비색법(溶出比色法)에서는 4 ppm, densitometry에서는 0.1 ppm이 검출한계(檢出限界)이었다. 미곡 중의 허용량(許容量)을 3.68 ppm이하로 정설할 경우 곡류시료 중 luteoskyrin의 검색(檢索)에는 densitometry가 적합할 것이다. 3) Pen. islandicum에 의한 luteoskyrin 생성능은 Czapek 액체배지에서는 균계체(菌系體) 1g당 11mg, 가압살균한 쌀에서는 1g당 40mg이었다.

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국내산 및 제초제 내성 콩의 단백질 조성 분석 (Protein Composition of Domestic and Glyphosate-Tolerant Soybean)

  • 위춘화;석대은;양윤형;오상희;김형진;윤원기;김환묵;김미리
    • 한국식품영양과학회지
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    • 제35권4호
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    • pp.470-475
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    • 2006
  • 국내산 콩 품종 3종과 미국산 콩 2종(non-GMO 및 GMO glyphosate-tolerant HS2906)에 대하여 단백질함량과 분포의 차이를 아미노산 분석, 총 질소량, PAGE/densitometry법에 의 해 분석하였다. 아미노산 구성과 총 질소량에서 품종간 유의적인 차이는 없었다. 한편 단백질 추출에서 SDS/buffer 추출법이 더 효과적이었다. 총 단백질함량(380.2-423.9 mg/g)과 단백질 분포는 품종간 유사하였으나. 상세 PACE(12.5% normal gel) 결과 재래종 콩(WS82)에 비해 제초제 저항성 GMO콩(HS2906)에서 beta conglycinin(55kDa) 양이 낮게 나타났으며, 25 kDa 단백질의 경우 미국산 콩에서는 관찰되지 못한 반면에 국내산 콩 품종에서는 뚜렷하게 관찰되었으며, ${\beta}$-conglycinin은 미국산 제초제 저항성 GMO콩과 국내산 황금콩에서 상대적으로 낮은 것으로 관찰되었다. 이와 같은 결과는 normal PAGE/densitometry 분석법은 콩시료 분석에 유용하게 활용될 수 있음을 보여주었다.

DEXA 측정기 간 골밀도 값 비교 (Comparison of the Values of Bone Mineral Density Between DEXAs)

  • 이인자
    • 대한방사선기술학회지:방사선기술과학
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    • 제34권4호
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    • pp.271-276
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    • 2011
  • 산부인과에서 2010년 1월 1일부터 2010년 5월 30일까지 5개월간 Lunar 장비와 Hologic 장비를 사용하여 골밀도 검사를 한 환자를 40, 50, 60대 각각 50, 100, 50명 각 200명, 400명에 대한 척추 L1-L4의 T-score값을 통계적 분석을 하였으며, 정상인 4명을 두 장비에 같은 날 골밀도 검사를 한 결과와 비교 분석을 한 결과는 다음과 같다. 두 장비로 검사한 평균 연령은 54.5세와 54.4세로 차이는 없었다. 이때 T-score는 Lunar 장비가 $-1.377{\pm}1.221$이며, Hologic 장비는 $-1.806{\pm}1.123$으로 Lunar 장비의 T-score가 높게 측정이 되었다. 두 장비 간 유의수준은 P=0.000으로 유의한 차이가 있는 것으로 나타났다. 또한 WHO의 기준에 맞추어 정상, 골감소증, 골다공증으로 분류해 본 결과 Lunar 장비로 검사한 경우 정상으로 판정된 경우가 35%로 많은 반면 Hologic 장비로 검사한 경우는 골다공증으로 판정된 경우가 28%로 많았다. 이에 정상인 4명을 동일 장비에 검사한 경우 L1-L4의 T-score 값을 비교해 본 결과 Lunar 장비에서 T-score는 $-0.4{\pm}1.192$, Hologic 장비는 $-1.1{\pm}1.030$으로 나타나 역시 Lunar 장비의 T-score 값이 높게 측정되었다. 따라서 두 측정기 간 T-score 값이 다르므로 보정인자를 시용해야 할 것으로 사료된다.

포장 인쇄의 인쇄적성 향상에 관한 연구 (The Study of Quality Control for Package Printing)

  • 이만교;하영백;윤종태
    • 한국인쇄학회지
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    • 제23권1호
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    • pp.53-63
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    • 2005
  • The quality of the print can be specified through the quality of the coloring, the reproduction of fine structures and the range of tone values. Also, package printed quality are controlled by this points. In this paper, densitometry method was used for printed quality. By densitometry we can get the print density and the parameters of each conditions that characterize halftone printing, such as dot gain and relative printing contrast. Also, we have proposed the optimizes range of the parameters such as density, dot gain etc. to the package printed printability control.

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석이중 오르시놀 정량 및 급성독성 (Quantitative Analysis of Orcinol and Acute Toxicity of Gyrophora esculenta)

  • 최혁재;김남재;김동현
    • 약학회지
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    • 제45권2호
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    • pp.169-179
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    • 2001
  • In previous study, Gyrophora esculenta showed significant inhibitory effect on $\alpha$-glucosidases in vitro and blood glucose elevation in vivo. In the isolating process of active substance, orcinol was separated from Gyrophora esculenta. Orcinol is known to be toxic, therefore, in this study, it was analysed by the TLC densitometry method for quantitative determination from Gyrophora esculenta. The average amount of orcinol of Gyrophora esculenta was 0.2%. For the purpose of removing orcinol, the water extract of Gyrophora esculenta was sequentially fractionated by organic solvents, and the acute toxicity of each fraction was assessed in mice. Among them, the LD50 of butanol fraction was 1.19 g/kg(p.o.) and the weight increase of mice in that group was somewhat retarded.

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Incidentally detected abnormal finding of femoral bone density image due to treatment of postoperative lymphatic leakage

  • Han-Kyung Seo;Do-Cheol Choi;Jae-Yung Jeong;Cheol-Min Shim;Yung-Hwan Jeong
    • 핵의학기술
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    • 제28권1호
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    • pp.13-17
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    • 2024
  • Bone Mineral Densitometry (BMD) values is determined numerically with T or Z-score while the image is not considered in the interpretation. Abnormal finding was observed in the left femoral bone density image of a 64-year-old female patient who underwent a left kidney transplantation. This was due to the ethiodized oil used for embolization in the treatment of lymphatic leakage. Radiologic technologists should not only monitor changes of BMD values, but keep in mind to assess the changes in the image to provide accurate information to the patient.

밀도법 및 피부두겹법에 의한 중년부인의 총지방량 측정 (Total Body Fat Estimated by Means of Densitometry and Skinfold Thickness Method in Middle Aged Housewives)

  • 최덕경;신효숙;황애련
    • The Korean Journal of Physiology
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    • 제2권1호
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    • pp.89-93
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    • 1968
  • Total body fat measurements were performed in 342 housewives. In 44 of them(age 38.4 yr.) both the densitometry and skinfold thickness methods were made and regression equations between skinfold and total body fat were derived. In the 298 housewives(age 35.0 yr.) skinfold thickness at four sites (arm, back, waist, abdomen) were measured and total body fat was calculated. The following results were obtained. 1. The data in 44 subjects by means of densitometry and skinfold thickness were: body weight: 51.8 kg, body length: 153.4 cm, body surface area: $1.47\;m^2$, body volume: 50.2 l, body density: 1.0334 kg/l, total body fat: 25.2% body weight, mean skinfold thickness at four sites 14.9 mm. 2. There were correlations of high degree between skinfold thickness and fat (%), fat (kg), and body density. The correlation coefficients were r=0.767, r=0.846, r= -0.765, respectively. Subsequently, the following regression equations were obtained. %fat=$0.39{\times}Mean$ skinfold thickness(mm)+19.36 Fat(kg)=$0.414{\times}Mean$ skinfold thickness(mm)+7.01 Body density=$-0.00099{\times}Mean$ skinfold thickness(mm)+1.0489 3. In 298 subjects mean skinfold thickness of four sites was 20.6mm and total body fat was calculated as 27.4% body weight from the above equation.

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Protein Profiles of Major Korean Rice Cultivars

  • Wei, Chuanha;Kwon, Oh-Yun;Liu, Xi-Wen;Kim, Hyoung-Chin;Yoon, Won-Kee;Kim, Hwan-Mook;Kim, Mee-Ree
    • Preventive Nutrition and Food Science
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    • 제12권2호
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    • pp.103-110
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    • 2007
  • The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility fractionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14${\sim}$16 kDa albumin and 22 kDa globulin ${\alpha}$-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14${\sim}$16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.

DEXA사에 의한 일부 남자농구선수들의 체구성에 관한 연구 -체지방량, 체구성, 비율, 체조직 구성 비율, 골광물질함량과 골밀도를 중심으로- (A Study on the Body Composition in Korean Basketball Players by Dual Energy X -ray Absorptiometry)

  • 박경래;강동원;최중명;박순영
    • 보건교육건강증진학회지
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    • 제15권1호
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    • pp.179-194
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    • 1998
  • This study focused on the body composition of Korean Basketball Players in Dual X -ray Absorptiometry. The principal subjects of this study were 10 Basketball Players who participated in the process of Dual Energy X -ray Absorptiometry at K.H. University Hospital for 8 months from Sept. 1. 1996 to April, 30. 1997. On the basis of the these measurements, the authors calculated physical indices and total fat percent. On the results of bone densitometry, the author analyzed body fat weight, body composition according to body position, bone mineral density and discerned the difference between the fatness which was calculated indirectly and which gained from the bone densitometry. 1. General Characteristics The mean age of the subjects was 20.4$\times$1.35 year. 2. Physical. Measurement The mean physical growth and development of the subjects were superior to standard value of the average Koreans 3. Body Fat by Physical Measurement Body surface area was 2.019$\times$0.111$m^2$, body volume was 74.4$\times$7.2$\ell$, body density was 1.041$\times$0.007$\ell$/kg, and body fat percent was 24.9$\times$2.9%. 4. Body Fat by Bone Densitometry Total body fat percent was 15.17$\times$2.19% and according to body position that of upper limb was 1.62%, that of lower limb was 5.55%, that of trunk was 7.06% and that of head was 1.05%. There was significant difference between the amounts of body fat from the methods used in this study; that from physical measurements and from bone densitometry method could be said most desirable. 5. Body composition According to Body Position Trunk was highest at 46.7%, lower limb was 36.0%, upper limb was 10.6%, and head was 6.7% in order. 6. Bone Mineral Content and Bone Density In bone mineral amount by body position, that of upper limb was 466.9$\times$46.4g, that of lower limb was 1,424.1$\times$154.0g, that of trunk was 1,343.0$\times$150.3g, and total bone mineral content was 3,786.8$\times$348.4g(4.78$\times$0.13%). Bone mineral density by body position, that of upper limb was 0.758$\times$0.072g/$\textrm{cm}^2$, that of lower limb was 1,342$\times$0.095g/$\textrm{cm}^2$, that of trunk was 1,169$\textrm{cm}^2$0.082g/$\textrm{cm}^2$, that of head was 1,742$\times$0.154g/$\textrm{cm}^2$ and total bone mineral density was 1,204$\times$0.077g/$\textrm{cm}^2$.

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