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http://dx.doi.org/10.3746/jfn.2007.12.2.103

Protein Profiles of Major Korean Rice Cultivars  

Wei, Chuanha (College of Pharmacy, Chungnam National University)
Kwon, Oh-Yun (Dept. of Food and Nutrition, Chungnam National University)
Liu, Xi-Wen (College of Pharmacy, Chungnam National University)
Kim, Hyoung-Chin (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
Yoon, Won-Kee (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Hwan-Mook (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Mee-Ree (Dept. of Food and Nutrition, Chungnam National University)
Publication Information
Preventive Nutrition and Food Science / v.12, no.2, 2007 , pp. 103-110 More about this Journal
Abstract
The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility fractionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14${\sim}$16 kDa albumin and 22 kDa globulin ${\alpha}$-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14${\sim}$16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.
Keywords
rice cultivar; protein profile; solubility fractionation; SDS-PAGE;
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