• 미생물학회지
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• 제38권1호
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• pp.7-12
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• 2002

Fusarium section Liseola에 속하는 균주들의 rDNA IGS 부위를 중폭하고 여러개의 제한 효소로 처리하였다. 증폭된 IGS 부위의 길이는 F. moniliforme 12 만이 약 2.9 Kb 이고 나머지 균주들은 모두 약 2.6 Kb였다. 제한 효소 EcoRI, HincII, SalI, PstI 등은 ICS 부위를 절단하여 11균주들에서 9 haplotypes을 확인할 수 있었다. 본 연구에서의 Section Liseola에 속하는 균주들에 대한 결과에 앞서 연구되어진 Section Elegans에 속하는 균주들의 결과를 종합하여 dendrogram을 그렸을 때, IGS 부위를 종내에서와 마찬가지로 종간, section 간의 관계를 밝힐 수 있는 가능성을 제시하여 주었다.

• 한국자원식물학회지
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• 제25권3호
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• pp.308-316
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• 2012

The purpose of this study was to identify the variant of Platanus occidentalis, whose bark looks white, also can be classified as P. occidentalis and to examine its genetic difference from the general P. occidentalis. For the variant identification of P. occidentalis, SNP and ISSR analysis were used in this study. Thirteen samples of P. occidentalis white variant were collected in Cheongju and 24 samples of normal P. occidentalis obtained in Cheongju, Pyongtaek, Ansan, Suwon, Osan and Jincheon area. ITS 1 and ITS 2 sequences of white variants were identical with those of P. occidentalis. We could not find any sequence difference between normal and white P. occidentalis. So we concluded that the white variant belongs to normal P. occidentalis even their bark is white and peeled easily. By ISSR test, 98 amplicons were acquired using 10 primers. P. occidentalis and white P. occidentalis showed different band patterns from the UBC #834. According to the result of Nei (1979)'s genetic distance analysis, the members of white P. occidentalis were grouped more tightly than the members of normal P. occidentalis. The UPGMA dendrogram shows that the variant and P. occidentalis divided widely into two groups. These results show that the phenotype of P. occidentalis white variant is caused by genetic factors rather than by environmental factors.

• 한국발생생물학회지:발생과생식
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• 제24권3호
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• pp.241-248
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• 2020

This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp. The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01-RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.

• 산업식품공학
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• 제21권2호
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• pp.174-179
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• 2017

For preliminary molecular typing, PCR-based fingerprinting using random amplified polymorphic DNA (RAPD) is the method of choice. In this study, 14 bacterial strains were isolated from different Korean food sources, identified using 16S rRNA gene sequencing, and characterized through RAPD-PCR. Two PCR primers (239 and KAY3) generated a total of 130 RAPD bands, 14 distinct PCR profiles, 10 polymorphic bands, one monomorphic band, and four unique bands. Dendrogram-based analysis with primer 239 showed that all 14 strains could be divided into seven clades out of which clade VII had the maximum of seven. In contrast, dendrogram analysis with the primer KAY3 divided the 14 L. citreum strains into four clades out of which clade IV consisted of a maximum of 10 strains out of 14. This research identified and characterized bacterial populations associated with different Korean foods. The proposed RAPD-PCR method, based on sequence amplification, could easily identify and discriminate the lactic acid bacteria species at the strain-specific level and could be used as a highly reliable genomic fingerprinting tool.

• 한국발생생물학회지:발생과생식
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• 제23권2호
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• pp.193-198
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• 2019

Genomic DNA samples were obtained from cultured penaeid shrimp (Penaeus chinensis) individuals such as fresh shrimp population (FSP) and deceased shrimp population (DSP) from Shinan regions in the Korean peninsula. In this study, 233 loci were identified in the FSP shrimp population and 162 in the DSP shrimp population: 33 specific loci (14.2%) in the FSP shrimp population and 42 (25.9%) in the DSP population. A total of 66 (an average of 9.4 per primer) were observed in DSP shrimp population, whereas 55 unique loci to each population (an average of 7.9 per primer) in the FSP shrimp population. The Hierarchical dendrogram extended by the seven oligonucleotides primers indicates three genetic clusters: cluster 1 (FRESH 01, 02, and DECEASED 12, 13, 15, 16, 17, 19, 20, 22) and cluster 2 (FRESH 03, 04, 05, 06, 07, 08, 09, 10, 11, and DECEASED 14, 18, 21). Among the twenty-two shrimp, the shortest genetic distance that exposed significant molecular differences was between individuals 20 and 16 from the DSP shrimp population (genetic distance=0.071), while the longest genetic distance among the twenty-two individuals that established significant molecular differences was between individuals FRESH no. 02 and FRESH no. 04 (genetic distance=0.477). In due course, PCR analysis has revealed the significant genetic distance among two penaeid shrimp populations.

• 한국발생생물학회지:발생과생식
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• 제23권3호
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• pp.297-304
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• 2019

The higher fragment sizes (>2,100 bp) are not observed in the two C. spp. populations. The six oligonucleotides primers OPA-11, OPB-09, OPB-14, OPB-20, OPC-14, and OPC-18 were used to generate the unique shared loci to each tonguesole population and shared loci by the two tonguesole populations. The hierarchical polar dendrogram indicates two main clusters: Gunsan (GUNSAN 01-GUNSAN 11) and the Atlantic (ATLANTIC 12-ATLANTIC 22) from two geographic populations of tonguesoles. The shortest genetic distance displaying significant molecular difference was between individuals' GUNSAN no. 02-GUNSAN no. 01 (genetic distance=0.038). In the long run, individual no. 02 of the ATLANTIC tonguesole was most distantly related to GUNSAN no. 06 (genetic distance=0.958). These results demonstrate that the Gunsan tonguesole population is genetically different from the Atlantic tonguesole population. The potential of PCR analysis to identify diagnostic markers for the identification of two tonguesole populations has been demonstrated. As a rule, using various oligonucleotides primers, this PCR method has been applied to identify polymorphic/specific markers particular to species and geographical population, as well as genetic diversity/polymorphism in diverse species of organisms.

• 한국발생생물학회지:발생과생식
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• 제26권2호
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• pp.91-98
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• 2022

Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONT-primers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity's no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (CHARYBCRAB 01, 04, 05, 06, and 08), cluster II (CHARYBCRAB 02, 03, 07, 09, 10, and 11) and cluster III (CHARYBCRAB 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The shortest genetic distance (GD) displaying significant molecular difference (MD) was between individuals CHARYBCRAB no. 18 and CHARYBCRAB no. 17 (0.055).

• 한국발생생물학회지:발생과생식
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• 제25권3호
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• pp.185-192
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• 2021

The five oligonucleotide primers (oligo-primers) turned out a total of 335 fragments (FMs) (52.9%) in the blue crab (Portunus trituberculatus) group alpha and 298 FMs (47.1%) in the crab group beta, with the FM scales range varying from 100 bp to 2,000 bp. The highest band-sharing (BS) value (0.907) was found between individual's no. 19 and no. 20 within the blue crab group beta. Parties in the blue crab group beta (0.601±0.017) had higher BS rates than did parties from the crab group alpha (0.563±0.017) (p<0.05). The polar dendrogram got by the five oligo-primers points out two genetic extents: bundle I (BLUECRAB 01, 03, 04, 05, 06, 08, and 10) and bundle II (BLUECRAB 02, 07, 09. 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The OPD-01 primer revealed 22 loci shared by all the examples of the as FMs of 1,000 bp. The oligo-primer OPA-05 made unique loci shared to each group (ULSEG), almost 400 bp and 500 bp, individually, in blue crab group beta. The remaining oligo-primers did not reveal any loci shared by the two crab groups (LSTG). The average number of ULSEG was diverse and 1.6-fold higher in the crab group beta than in the crab group alpha.

• 한국발생생물학회지:발생과생식
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• 제25권4호
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• pp.305-311
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• 2021

The oligonucleotides polymers (ON-polymers) were used producing a total of 110 loci unique to each clam population (LUECP) in group one and 132 in group two, respectively, varying in amount of DNA fragments (FRs) from greater than near 50 to a smaller quantity than 1,050 bp. The larger FR amounts (>1,050 bp) are not noticed in the two Scapharca subcrenata groups. The ON-polymer OPD-01 produced 33 LUECP, which were defining each group, almost 300 bp, 450 bp, and 500 bp, in the group one. The OPD-15 recognized 22 loci shared by the two clam populations (Loci shared by the two clam populations, LSTCP), a variety of FRs of sizes 300 bp that were equivalent in all specimens. The mean number of LUECP was varied and 1.2-fold greater in the shellfish group two than in the group one. Respecting mean bandsharing (BS) grade outcomes, entities in the shellfish group one (0.779±0.011) had a little higher BS grades than did entities from the group two (0.756±0.009) (p<0.05). The entities of the shellfish group one are not tightly gathered with other entities of the group two. The genetic distance (GD) (0.422) of this invertebrate (SUBCRENATA 02 and 01) is 7.41-fold hereditarily distinct to the GD (0.057) of the other invertebrate (SUBCRENATA 22 and 19). The polar dendrogram (PDG) procured by the five ON-polymers underlines two characteristic groups.

• 한국잡초학회지
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• 제5권2호
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• pp.96-102
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• 1985

최근에 발생빈도가 늘어가고 있는 것으로 알려진 올챙고랭이를 중심으로 하여 논에 빈발하는 10개 잡초종의 경합생태적 특성을 정리 분류할 목적(目的)으로 단식(單植)과 벼 입묘하(立苗下)에 공시하였다. 분류 및 해석은 공시잡초종의 공간경합성과 양분경합성, 이들 잡초종에 대한 벼의 공간경합성과 양분경합성 및 수량성 반응을 기초로 하는 Singk-link-cluster 법(法)에 의하였다. 1. 잡초종들의 생장특성은 단식(單植)과 입묘하(立苗下)에서 대체로 유사한 경향(r= $0.969^{**}$) 이었으며 공간점유성이 큰 초종(草種)(피 * 여뀌바는 등)이 영양수탈력도 큰편이었으나(r=$0.712^*$), 벼의 종간경합으로 인한 생육억제율은 오히려 커지는 경향이었다(r-0.513). 2. 잡초의 종내 및 종간경합성에 의한 분류결과는 피 여뀌바늘 너도방동산이 및 매자기가 한 무리, 올망개 올챙고랭이가 한 우리, 그러고 골풀과 가래가 다른 우리를 이루는 경향이었다. 3. 잡초에 대한 벼의 종간경합성과 수량반응을 기초로 분류한 결과는 바람하늘직이 올챙고랭이 올망개 매자기 및 골풀이 한 무리로, 그러고 여뀌 바늘과 가래 및 물달개비가 다른 우리로 나타났다. 4 잡초 및 벼의 종간경합에 따른 경합성을 종합적으로 분류한 결과, 생장특성은 크나 벼 경합력은 낮은 바람하늘직이 올챙고랭이 올방개 골풀이 제 I 군, 모두가 큰 너도방동산이 피 가래 여뀌바늘이 제 II군, 생장특성은 작아도 경합력은 큰 물달개비와 매자기가 제 III군으로 구분될 수 있었다.