Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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PCR-RFLP Analysis of Ribosomal DNA Intergenic Spacer Region in Fusarium section Liseola.

Fusarium section Liseola 균주들에서 rDNA Intergenic Spacer 부위의 PCR-RFLP 분석

  • 이경은 (상명대학교 자연과학부 생명과학과) ;
  • 최영길 (한양대학교 자연과학부 생명과학과) ;
  • 민병례 (상명대학교 자연과학부 생명과학과)
  • Published : 2002.03.01
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Abstract

The intergenic spacer (IGS) region of the ribosomal DNA of species in Fusarium section Liseola was analyzed by amplification and subsequent digestion with several restriction enzymes. The length of the amplified IGS region was about 2.6 Kb in all strains except F.moniliforme 12 Which was about 2.9 Kb. The enzymes, EcoRI, HincII, SalI, HindIII, PstI and SmaI, digested the IGS region and nine haplotypes were identified among 11 strains. In the dendrogram based on PCR-RFLP of IGS region combined the results of section Liseola in this study and section Elegans in previous study, variation in the IGS appears to offer considerable potential to resolve intraspecific relationship as well as interspecies or intersection.

Fusarium section Liseola에 속하는 균주들의 rDNA IGS 부위를 중폭하고 여러개의 제한 효소로 처리하였다. 증폭된 IGS 부위의 길이는 F. moniliforme 12 만이 약 2.9 Kb 이고 나머지 균주들은 모두 약 2.6 Kb였다. 제한 효소 EcoRI, HincII, SalI, PstI 등은 ICS 부위를 절단하여 11균주들에서 9 haplotypes을 확인할 수 있었다. 본 연구에서의 Section Liseola에 속하는 균주들에 대한 결과에 앞서 연구되어진 Section Elegans에 속하는 균주들의 결과를 종합하여 dendrogram을 그렸을 때, IGS 부위를 종내에서와 마찬가지로 종간, section 간의 관계를 밝힐 수 있는 가능성을 제시하여 주었다.

Keywords

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