Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

Genetic Distances of Rainbow Trout and Masu Salmon as Determined by PCR-Based Analysis

  • Yoon, Jong-Man (Dept. of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • Received : 2020.07.17
  • Accepted : 2020.09.11
  • Published : 2020.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study used a PCR-based genetic analysis platform to create a hierarchical polar dendrogram of Euclidean genetic distances for two salmonid species, Oncorhynchus mykiss (rainbow trout, RT) and Oncorhynchus masou (masu salmon, MS). The species were distantly related to other fish species based on PCR results from using the designed oligonucleotide primer series. Five oligonucleotide primers were used to generate 330 and 234 scorable fragments in the RT and MS populations, respectively. The DNA fragments ranged in size from approximately 50 bp to more than 2,000 bp. The bandsharing (BS) results showed that the RT population had a higher average BS value (0.852) than that for the MS population (0.704). The genetic distance between individuals supported the presence of adjacent affiliation in cluster I (RT 01-RT 11). The observation of a significant genetic distance between the two Oncorhynchus species verifies that this PCR-based technique can be a useful approach for individual- and population-based biological DNA investigations. The results of this type of investigation can be useful for species safekeeping and the maintenance of salmonid populations in the mountain streams of Korea.

Keywords

References

  1. Baik KK, Choi YH, Lee JC, Park IS, Kim YK, Kim DY, Lee CS (2007) Studies on seed production of rainbow trout, Oncorhynchus mykiss: Hatching rate and early stage performance of USA strain rainbow trout, Oncorhynchus mykiss. J Aqua 20:85-89.
  2. Cagigas ME, Vazquez E, Blanco G, Sanchez JA (1999) Combined assessment of genetic variability in populations of brown trout (Salmo trutta L.) based on allozymes, microsatellites, and RAPD markers. Mar Biotechnol 1:286-296. https://doi.org/10.1007/PL00011778
  3. Callejas C, Ochando MD (1998) Identification of Spanish barbel species using the RAPD technique. J Fish Biol 53:208-215. https://doi.org/10.1111/j.1095-8649.1998.tb00121.x
  4. Chenyambuga SW, Hanotte O, Hirbo J, Watts PC, Kemp SJ, Kifaro GC, Gwakisa PS, Petersen PH, Rege JEO (2004) Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers. Asian-Australas J Anim Sci 17:445-452. https://doi.org/10.5713/ajas.2004.445
  5. Gwakisa PS, Kemp SJ, Teale AJ (1994) Characterization of zebu cattle breeds in Tanzania using random amplified polymorphic DNA markers. Anim Genet 25:89-94. https://doi.org/10.1111/j.1365-2052.1994.tb00433.x
  6. Huang BX, Peakall R, Hanna PJ (2000) Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers. Mar Biol 136:207-216. https://doi.org/10.1007/s002270050678
  7. Islam MS, Ahmed ASI, Azam MS, Alam MS (2005) Genetic analysis of three river populations of Catla catla (HAMILTON) using randomly amplified polymorphic DNAs markers. Asian-Australas J Anim Sci 18:453-457. https://doi.org/10.5713/ajas.2005.453
  8. Jeffreys AJ, Morton DB (1987) DNA fingerprints of dogs and cats. Anim Genet 18:1-15. https://doi.org/10.1111/j.1365-2052.1987.tb00739.x
  9. Kim JY, Park CY, Yoon JM (2004) Genetic differences and DNA polymorphism in oyster (Crassostrea spp.) analysed by RAPD-PCR. Korean J Genet 26:123-134.
  10. Kim KH, Kang SI, Jeon YJ, Choi BD, Kim MW, Kim DS, Kim JS (2014) Food quality of rainbow trout Oncorhynchus mykiss domesticated in seawater. Korean J Fish Aquat Sci 47:114-121. https://doi.org/10.5657/KFAS.2014.0114
  11. Liu Z, Li P, Argue BJ, Dunham RA (1998) Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. Furcatus) and their F1, F2 and backcross hybrids. Anim Genet 29:58-62. https://doi.org/10.1046/j.1365-2052.1998.00284.x
  12. Mamuris Z, Stamatis C, Bani M, Triantaphyllidis C (1999) Taxonomic relationships between four species of the Mullidae family revealed by three genetic methods: Allozymes, random amplified polymorphic DNA and mitochondrial DNA. J Fish Biol 55:572-587. https://doi.org/10.1111/j.1095-8649.1999.tb00700.x
  13. McCormack GC, Powell R, Keegan B (2000) Comparative analysis of two populations of the brittle star Amphiura filiformis (Echinodermata: Ophiuroidae) with different life history strategies using RAPD markers. Mar Biotechnol 2:100-106. https://doi.org/10.1007/s101269900013
  14. Muchmore ME, Moy GW, Swanson WJ, Vacquier VD (1998) Direct sequencing of genomic DNA for characterization of a satellite DNA in five species of Eastern Pacific abalone. Mol Mar Biol Biotech 7:1-6.
  15. Myoung JG, Kim YU (1993) Morphological study of Oncorhynchus spp. (Pisces: Salmonidae) in Korea- IV. Comparison of morphological characters of chum salmon O. keta, masu salmon O. masou and rainbow trout O. mykiss. Korean J Ichthyol 5:96-112.
  16. Myoung JG, Kim YU (1996) Morphological study of Oncorhynchus spp. in Korea- V. Comparison of skeletal characters of chum salmon O. keta, masu salmon O. masou and rainbow trout O. mykiss. J Korean Fish Soc 29:208-229.
  17. Oh H, Yoon JM (2014) Genetic distances of three mollusk species investigated by PCR analysis. Dev Reprod 18:43-49. https://doi.org/10.12717/DR.2014.18.1.043
  18. Park SY, Park JS, Yoon JM (2005) Genetic differences and variations in slipper lobster (Ibacus ciliatus) and deep sea lobster (Puerulus sewelli) determined by RAPD analysis. Korean J Genet 25:307-317.
  19. Partis L, Wells RJ (1996) Identification of fish species using random amplified polymorphic DNA (RAPD). Mol Cell Probes 10:435-441. https://doi.org/10.1006/mcpr.1996.0060
  20. Sohn SG, Park MA, Park JW (1993) Studies on viral disease of masu salmon, Oncorhynchus masou-II Isolation of infectious hematopoietic necrosis virus from masu salmon fry. J Fish Pathol 6:87-92.
  21. Song YJ, Yoon JM (2013) Genetic differences of three Pollicipes mitella population identified by PCR analysis. Dev Reprod 17:199-205. https://doi.org/10.12717/DR.2013.17.3.199
  22. Tassanakajon A, Pongsomboon S, Jarayabhand P, Klinbunga S, Boonsaeng V (1998) Genetic structure in wild populations of black tiger shrimp (Penaeus monodon) using randomly amplified polymorphic DNA analysis. J Mar Biotechnol 6:249-254.
  23. Yoke-Kqueen C, Radu S (2006) Random amplified polymorphic DNA analysis of genetically modified organisms. J Biotechnol 127:161-166. https://doi.org/10.1016/j.jbiotec.2006.06.001
  24. Yoon JM (2002) Ultrastructural change of osmoregulatory cells during seawater adaptation in rainbow trout (Oncorhynchus mykiss). Korean J Ichthyol 12:111-117.
  25. Yoon JM (2018) Genetic distances of Paralichthys olivaceus populations investigated by PCR. Dev Reprod 22:283-288. https://doi.org/10.12717/DR.2018.22.3.283
  26. Yoon JM, Kim GW (2003a) Genetic differences between cultured and wild penaeid shrimp (Penaeus chinensis) populations analysed by RAPD-PCR. Korean J Genet 25:21-32.
  27. Yoon JM, Kim YH (2003b) Wide marsh clam (Corbicula spp) populations from three sites analysed by RAPD-PCR-AGE. Bull Electrochem 19:337-348.
  28. Yoon JM, Park HY (2002) Genetic similarity and variation in the cultured and wild crucian carp (Carassius carassius) estimated with random amplified polymorphic DNA. Asian-Australas J Anim Sci 15:470-476. https://doi.org/10.5713/ajas.2002.470
  29. Yoon JM, Kim JY (2004) Genetic differences within and between populations of Korean catfish (S. asotus) and bullhead (P. fulvidraco) analysed by RAPD-PCR. Asian-Australas J Anim Sci 17:1053-1061. https://doi.org/10.5713/ajas.2004.1053
  30. Zhou L, Wang Y, Gui JF (2000) Analysis of genetic heterogeneity among five gynogenetic clones of silver crucian carp, Carassius auratus gibelio Bloch, based on detection of RAPD molecular markers. Cytogenet Cell Genet 88:133-139. https://doi.org/10.1159/000015506