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PCR-RFLP Analysis of Ribosomal DNA Intergenic Spacer Region in Fusarium section Liseola.  

이경은 (상명대학교 자연과학부 생명과학과)
최영길 (한양대학교 자연과학부 생명과학과)
민병례 (상명대학교 자연과학부 생명과학과)
Publication Information
Korean Journal of Microbiology / v.38, no.1, 2002 , pp. 7-12 More about this Journal
Abstract
The intergenic spacer (IGS) region of the ribosomal DNA of species in Fusarium section Liseola was analyzed by amplification and subsequent digestion with several restriction enzymes. The length of the amplified IGS region was about 2.6 Kb in all strains except F.moniliforme 12 Which was about 2.9 Kb. The enzymes, EcoRI, HincII, SalI, HindIII, PstI and SmaI, digested the IGS region and nine haplotypes were identified among 11 strains. In the dendrogram based on PCR-RFLP of IGS region combined the results of section Liseola in this study and section Elegans in previous study, variation in the IGS appears to offer considerable potential to resolve intraspecific relationship as well as interspecies or intersection.
Keywords
Fusarium; haplotype; IGS; PCR-RFLP; restriction enzyme;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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1 Current status of taxonomy in Fusarium species and their perfect stages /
[ Synder, W.C;Y.A. Toussoun ] / Phytopathol.
2 Study of electrophoretic Karyotype in section Liseola Fusarium /
[ Min, B.R.;M.S. Ahn;Y.K. Choi ] / K. J. Microbiol.   과학기술학회마을
3 Identification of Fusaria occuring in Europe on cereals and potatoes /
[ Nirenberg, H.I.;J. Chelkowski(ed.) ] / Fusarium mycotoxins, taxonomy and pathogenicity
4 Amplification and direct sequencing of fungal ribosomal genes from phylogenetics /
[ White, T.T.;T.D. Bruns;S. Lee;J. Taylor;Innis M.A.(ed.);Gel frand D.H(ed.);Sninsky J.J.(ed.);White T.J.(ed) ] / PCR protocols
5 Relationships among pathogenic and nonpathogenic isolates of Fusarium oxysporum based on the partial sequence of the intergenic spacer region of the ribosomal DNA /
[ Appel, D.J.;T.R. Gordon ] / MPMI   DOI   ScienceOn
6 Ribosomal DNA: molecular evolution and phylogenetic inference /
[ Hills, M.D.;M.T. Dixon ] / Q. Rew. Biol.   DOI   ScienceOn
7 Varieties of Gibberella fujikeuroi with anamorphs in Fusarium section Liseola /
[ Kuhlman, E.G. ] / Mycologia   DOI
8 Intraspecific variation within populations of Fusarium oxysporum based on RELP analysis of the intergenic spacer region of the rDNA /
[ Appel, D.J.;T.R. Gordon ] / Exp. Mycol.   DOI   ScienceOn
9 A genetic map of Gibberella fujikuroi mating population A (Fusarium moniliforme) /
[ Xu, Jin-rong;J.F. Lesle ] / Genetics
10 Rapid identification of genetic variation of Ectomycorrhizal fungi by amplification of ribosomal RNA genes /
[ Henrion, B.;F. LeTacon;F. Martin ] / New Phytol.   DOI   ScienceOn
11 Complete sequence of the 43Kb human ribosomal DNA repeat: analysis of the intergenic spacer /
[ Gonzalez, I.L.;J.E. Sylvestei ] / Genomics   DOI   ScienceOn
12 /
[ Booth, C. ] / The genus Fusarium
13 Ribosomal DNA restriction fragmet length polymorphism in Rhizoctonia solani /
[ Vilgaly, S.R.;D. Gonzalez ] / Phytopathol.   DOI
14 Genetic diversity of Fusarium oxysporum strains from common bean fieds in Spain /
[ Alves-Santos, F.M.;E.P. Benito;A.P. Eslava;J.M. Diaz-Minguez ] / Appl. Environ. Microbiol.
15 Populations of nonpathogenic Fusarium oxysporum associated with roots of four plant species compared to soil born populations /
[ Edel, V.;C. Steinberg;N. Gautheron;C. Alabouvette ] / Phytopathol.   DOI   ScienceOn
16 Genetic variation in Fusarium oxysporum from cyclamen /
[ Woudt, L.P.;A. Neuvel;A. Sikkema;M.Q.J.M. Van Grinsven;W.A.J. de Milliano;C.L. Campbell;J.F. Leslie ] / Phytopathol.   DOI
17 Restriction fragment length polymorphisms in isolates of Aspergillus fumigatus probed with part of the intergenic spacer region from the ribosomal RNA gene complex of Aspergillus nidulans /
[ Speradbury, C.L.;B.W. Bainbridage;J. Cohen ] / J. Gen. Microbiol.   DOI   ScienceOn
18 Current Status of Fusarium taxonomy /
[ Windels, C.E. ] / Phytopathol.
19 A multigene phylogeny of the Gibberella fujikuroi species complex:detection of additional phylogenetieilly distinct species /
[ O'Donell, K.;H.I. Nirenberg;T. Aoki;E. Cigelnik ] / Mycoscience   DOI   ScienceOn
20 The complete DNA sequence of the nuclear ribosomal RNA gene complex of Verticillium dahliae: Intraspecific heterogeneity within the intergence spacer region /
[ Paraskevi, V.P.;P.P. Antonion;M.A. Typas ] / Fungal Genet. Biol.   DOI   ScienceOn
21 Fungal molecular systematics /
[ Bruns, T.D.;T.J. White;J.W. Taylor ] / Annu. Rev. Ecol. Syst.   DOI   ScienceOn
22 The variation of intergenic spacer (IGS) region of ribosomal DNA among Fusarium oxysporum formae speciales /
[ Kim, H.J.;Y.K. Choi;B.R. Min ] / J. Microbiol.   과학기술학회마을
23 Electrophoretic Karyotypes difference between two subspecies of Fusarium acuminatum /
[ Nagy, R.;L. Hornok ] / Mycologia
24 Ribosomal DNA analysis challenge the status of Fusarium Section Liseola and Elegans /
[ Waalwijk, C.;R.P. Baayen;J.R.A. De Koning;W. Gams ] / Sydowia
25 PCR amplification of the 3' external transcribed and intergenic spacers of the ribosomal DNA repeat unit in three species of Saccharomyces /
[ Molina, F.I.;S. Jong;J.L.Huffmen ] / FEMS Microbio Lett.
26 Molecular systematics and phylogeoraphy of the Gibberella fujikuroi species complex /
[ O'Donell, K.;E. Cigelnik;H.I. Nirenberg ] / Mycologia   DOI   ScienceOn
27 Mating populations in Gibberella fujikuroi (Fusarium section Liseola) /
[ Leslie, J.F. ] / Phytopathol.
28 /
[ Nelson, P.E.;T.A. Toussoum;W.F.O. Marasas ] / Fusarium species: An illustrated manual for identification
29 Identification of Armillaria species from Hokkaido by analysis of the intergenic spacer (IGS) retion of ribosomal DNA using PCR-RELPs /
[ Terashima, K.;Y. Kawashima;J.Y. Cha;K. Miura ] / Mycoscience   DOI