• 한국양봉학회지
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• 제32권2호
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• pp.89-97
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• 2017

한국형 낭충봉아부패병 바이러스는 2009년 국내에 발생하여, 현재 국내 토봉의 99% 이상을 소멸시킨 주요 원인으로 추정되고 있다. 본 연구는 보고된 낭충봉아부패병 바이러스(SBV) 32종의 전체 염기서열을 비교 분석하여, 2,100 염기 부근에 특징적인 결실들(deletions)을 발견하였으며, 이에 기반으로 SBV의 유전자형을 제안하게 되었다. 제안된 유전자형에 의한 분류에서, 각 SBV의 지역성 및 범지역성, 감염특성 등에 유관함을 볼 수 있었다. 아울러, 제안된 이 유전자형은, 검역과 이후의 침임에 대한 방호를 위하여, kSBV의 기원도 바로 암시하고 있음을 우리는 잘 인식하고 있다.

• Molecules and Cells
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• 제19권1호
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• pp.114-123
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• 2005

Nitropyrene, the predominant nitropolycyclic hydrocarbon found in diesel exhaust, is a mutagenic and tumorigenic environmental pollutant that requires metabolic activation via nitroreduction and ring oxidation. In order to determine the role of ring oxidation in the mutagenicity of 1-nitropyrene, its oxidative metabolites, 1-nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide, were synthesized and their mutation spectra were determined in the coding region of hprt gene of CHO cells by a PCR amplification of reverse-transcribed hprt mRNA, followed by a DNA sequence analysis. A comparison of the two metabolites for mutation frequencies showed that 1-nitropyrene 9,10-oxide was 2-times higher than 1-nitropyrene 4,5-oxide. The mutation spectrum for 1-nitropyrene 4,5-oxide was base substitutions (33/49), one base deletions (11/49) and exon deletions (5/49). In the case of 1-nitropyrene 9,10-oxide, base substitutions (27/50), one base deletions (15/50), and exon deletions (8/50) were observed. Base substitutions were distributed randomly throughout the hprt gene. The majority of the base substitutions in mutant from 1-nitropyrene 4,5-oxide treated cells were $A{\rightarrow}G$ transition (15/33) and $G{\rightarrow}A$ transition (8/33). The predominant base substitution, $A{\rightarrow}G$ transition (11/27) and $G{\rightarrow}A$ transition (8/27), were also observed in mutant from 1-nitropyrene 9,10-oxide treated cells. The mutation at the site of adenine and guanine was consistent with the previous results, where the sites of DNA adduct formed by these compounds were predominant at the sites of purines. A comparison of the mutational patterns between 1-nitropyrene 4,5-oxide and 1-nitropyrene 9,10-oxide showed that there were no significant differences in the overall mutational spectrum. These results indicate that each oxidative metabolite exhibits an equal contribution to the mutagenicity of 1-nitropyrene, and ring oxidation of 1-nitropyrene is an important metabolic pathway to the formation of significant lethal DNA lesions.

• 대한화학회지
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• 제52권5호
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• pp.580-582
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• 2008

• 농업과학연구
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• 제36권2호
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• pp.159-165
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• 2009

본 연구는 현재까지 발표된 돼지의 genomic sequence 정보를 이용하여 PERV들의 정확한 삽입 위치를 파악하고 그들의 특성을 분석하고자 실시하였으며 총 2.7 Gb인 돼지 genome 염기서열 중 4.2%인 114 Mb의 염기서열에서 PERV sequence를 확인한 결과 총 8개의 PERV sequence를 확인할 수 있었다. 확인된 PERV sequence중 7개는 유전자내에 deletion이 확인되었으며 나머지 한 개의 PERV도 gag와 env 유전자에 stop codon이 확인되어 정상적인 PERV로 발현되지 않을 것으로 추정되었다. 본 연구는 돼지를 이용한 이종장기이식과 관련하여 PERV를 제어하기 위한 중요한 기초 연구 자료를 제공할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3535-3539
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• 2013

Head and neck cancer is one of the leading causes of deaths worldwide. Two genes GSTM1 and GSTT1 involved in phase II of carcinogen detoxification have been frequently studied in the literature. Their null genotypes are thought to be associated with increased head and neck cancer risk. However, the published reviews are not up to date and many important papers have been skipped. The current literature review was restricted to the null genotypes of the GSTM1 and GSTT1 genes with special emphasis on the genotypic status. We found that the size of study sample varied greatly and the oral cavity cancer was more influenced by GSTM1 and GSTT1 gene deletions. With respect to ethnicity Asians are more prone to head and neck cancers with these null genotypes as compared to Europeans and Americans. The current review showed significant associations (OR=9.0, 95%CI; 1.4-9.5; OR=3.7, 95%CI; 1.4-9.5) of GSTM1 and GSTT1 null genotypes with head and neck cancers. Review confirms the data of previous reviews that GSTM1 and GSTT1 gene polymorphisms may be risk factors for cancer initiation.

• Clinical and Experimental Pediatrics
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• 제58권8호
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• pp.313-316
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• 2015

Interstitial deletions involving the chromosome band 15q22q24 are very rare and only nine cases have been previously reported. Here, we report on a 12-day-old patient with a de novo 15q22q23 interstitial deletion. He was born by elective cesarean section with a birth weight of 3,120 g at 41.3-week gestation. He presented with hypotonia, sensory and neural hearing loss, dysmorphism with frontal bossing, flat nasal bridge, microretrognathia with normal palate and uvula, thin upper lip in an inverted V-shape, a midline sacral dimple, severe calcanovalgus at admission, and severe global developmental delay at 18 months of age. Fluorescence in situ hybridization findings confirmed that the deleted regions contained at least 15q22. The chromosome analysis revealed a karyotype of 46,XY,del(15) (q22q23). Parental chromosome analysis was performed and results were normal. After reviewing the limited literature on interstitial 15q deletions, we believe that the presented case is the first description of mapping of an interstitial deletion involving the chromosome 15q22q23 segment in Korea. This report adds to the knowledge of the clinical phenotype associated with the 15q22q23 deletion.

• 한국동물학회지
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• 제24권3호
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• pp.163-171
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• 1981

CHO 세포를 재료로 ENU와 MNU를 여러농도로 처리한후 시간 경과에 따른 염색체 이상율을 조사하여 다음과 같은 결과를 얻었다. (1) ENU와 MNU에 의한 염색체 이상 빈도는 처리 후 시간의 경과와 농도에 따라 상이한 현상을 나타냈다. (2) ENU 처리군에서는 염색체 이상형은 염색분체 절단이 대부분이었으나 고농도군 ($10^-3 M$)에서는 처리 후 24시간에 염색분체 교환이 염색분체 절단을 능가하였다. (3) MNU 처리군에서는 염색체 이상형은 염색분체 절단이 대부분이었으나 $10^-4$ M 이상에서는 처리후 12시간부터 염색분체 교환이 염색분체 절단을 능가하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권12호
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• pp.4915-4920
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• 2015

Background: : Familial adenomatous polyposis (FAP) is an autosomal dominant inherited disease mainly caused by mutations of the adenomatous polyposis coli (APC) gene with almost complete penetrance. These colorectal polyps are precancerous lesions that will inevitable develop into colorectal cancer at the median age of 40-year old if total proctocolectomy is not performed. So identification of APC germline mutations has great implications for genetic counseling and management of FAP patients. In this study, we screened APC germline mutations in Chinese FAP patients, in order to find novel mutations and the APC gene germline mutation characteristics of Chinese FAP patients. Materials and Methods: The FAP patients were diagnosed by clinical manifestations, family histories, endoscope and biopsy. Then patients peripheral blood samples were collected, afterwards, genomic DNA was extracted. The mutation analysis of the APC gene was conducted by direct polymerase chain reaction (PCR) sequencing for micromutations and multiplex ligation-dependent probe amplification (MLPA) for large duplications and/or deletions. Results: We found 6 micromutations out of 14 FAP pedigrees, while there were no large duplications and/or deletions found. These germline mutations are c.5432C>T(p. Ser1811Leu), two c.3926_3930delAAAAG (p.Glu1309AspfsX4), c.3921_3924delAAAA (p.Ile1307MetfsX13), c3184_3187delCAAA(p.Gln1061AspfsX59) and c4127_4126delAT (p.Tyr1376LysfsX9), respectively, and all deletion mutations resulted in a premature stop codon. At the same time, we found c.3921_3924delAAAA and two c.3926_3930delAAAAG are located in AAAAG short tandem repeats, c3184_3187delCAAA is located in the CAAA interrupted direct repeats, and c4127_4128 del AT is located in the 5'-CCTGAACA-3', 3'-ACAAGTCC-5 palindromes (inverted repeats) of the APC gene. Furthermore, deletion mutations are mostly located at condon 1309. Conclusions: Though there were no novel mutations found as the pathogenic gene of FAP in this study, we found nucleotide sequence containing short tandem repeats and palindromes (inverted repeats), especially the 5 bp base deletion at codon 1309, are mutations in high incidence area in APC gene,.

• 미생물학회지
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• 제28권1호
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• pp.13-18
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• 1990

adr아형 B형 간염바이러스의 preS2유전자 부위를lacZ 유전자의 5말단에 연결하여 preS2-$\beta$-galactosidase 융합단백질을 생성하는 플라스미드, pTSZ를 건설하였다. 갈본된 preS2 유전자의 3' 및 5발단을 결손시켜 얻은 재조합 플라스미드를 발현시킨 후 결손된 preS2를 포함하는 융합단백질의 항원성을 단일클론항체 H8을 사용하여 비교하며 보았다. 양말단에서 일정부위까지의 결손은 항원성에 영향을 미치지 않았지만 그 이상의 결손에 의하여는 항윈성이 소실됨을 볼 수 있었다. 이상의 항원성 전한부위를 DNA 염기서열 분석에 의하여 결정할 수 있었다. 그 결과 항원결정인자의 양말단은 preS2 서열 중 아미노산 전기 130-132와 140→142 사이에 각각 존재함을 알 수 있었고, 아미노산 143번의 결손은 항원성의 부분적인 감소를 초래하는 것으로 보아 항원성 결정에 보충적 역할을 한다고 생각된다. 한편 adr과 adw2아형 간의 아미노산서열의 차이가 항원결정부위 중 130, 132 및 141번 위치에 존재하며 단일를론항체 H8이 adr아형에만 특이하게 결합하는 것으로 부터, 세 잔기 중 하나 혹은 그 이상이 아형특이성에 관여한다고 추정된다.

• 대한음성학회:학술대회논문집
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• 대한음성학회 2002년도 11월 학술대회지
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• pp.161-164
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• 2002

This study is to investigate the phonetic and phonological characteristics in medieval galician portuguese. It is necessary to consider the phonetic and phonological changes from latin to galician portuguese to understand the phonetic and phonological characteristics in contemporary portuguese. This study considered the palatalization, the phonetic changes in consonant clusters, intervocalic consonant deletions, vowel diphthongizations and vowel nasalizations, which were major phonetic and phonological characteristics in medieval galician portuguese.