• 정보보호학회논문지
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• 제28권3호
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• pp.625-633
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• 2018

Realm은 모바일 기기에서 주로 사용되는 SQLite를 대체하기 위해 개발된 오픈 소스 데이터베이스이다. 데이터 베이스에 저장되는 데이터는 사용자의 행위를 파악하고 모바일 기기의 동작 여부를 확인하는 데 도움이 될 수 있어 모바일 기기를 대상으로 하는 디지털 포렌식 분석 과정에서 반드시 확인되어야 한다. 뿐만 아니라, 사용자가 의도적으로 데이터베이스에 저장된 데이터 삭제와 같은 안티 포렌식 기법을 사용할 수 있으므로 데이터베이스에서 삭제된 레코드를 복구하는 방법에 대한 연구가 필요하다. 본 논문은 Realm 데이터베이스 파일의 구조와 레코드의 저장 및 삭제 과정을 분석한 결과를 바탕으로 삭제된 후 덮어 쓰여지지 않은 레코드의 복구 기법을 제시하였다.

• The Plant Pathology Journal
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• 제30권4호
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• pp.367-374
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• 2014

Genomes contain a large number of unique genes which have not been found in other species. Although the origin of such "orphan" genes remains unclear, they are thought to be involved in species-specific adaptive processes. Here, we analyzed seven orphan genes (MoSPC1 to MoSPC7) prioritized based on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes formed during the evolutionary history of M. oryzae. Synteny analyses using closely related fungal species corroborated the notion that these genes evolved de novo in the M. oryzae genome. In this report, we discuss our inability to detect phenotypic changes in the four deletion mutants. Based on these results, the four orphan genes may be products of de novo gene birth processes, and their adaptive potential is in the course of being tested for retention or extinction through natural selection.

• The Plant Pathology Journal
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• 제36권4호
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• pp.314-322
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• 2020

Interplay between histone acetylation and deacetylation is one of the key components in epigenetic regulation of transcription. Here we report the requirement of MoHDA1-mediated histone deacetylation during asexual development and pathogenesis for the rice blast fungus, Magnaporthe oryzae. Structural similarity and phylogenetic analysis suggested that MoHDA1 is an ortholog of Saccharomyces cerevisiae Hda1, which is a representative member of class II histone deacetylases. Targeted deletion of MoHDA1 caused a little decrease in radial growth and large reduction in asexual sporulation. Comparison of acetylation levels for H3K9 and H3K14 showed that lack of MoHDA1 gene led to significant increase in H3K9 and H3K14 acetylation level, compared to the wild-type and complementation strain, confirming that it is a bona fide histone deacetylase. Expression analysis on some of the key genes involved in asexual reproduction under sporulation-promoting condition showed almost no differences among strains, except for MoCON6 gene, which was up-regulated more than 6-fold in the mutant than wild-type. Although the deletion mutant displayed little defects in germination and subsequent appressorium formation, the mutant was compromised in its ability to cause disease. Wound-inoculation showed that the mutant is impaired in invasive growth as well. We found that the mutant was defective in appressorium-mediated penetration of host, but did not lose the ability to grow on the media containing H₂O₂. Taken together, our data suggest that MoHDA1-dependent histone deacetylation is important for efficient asexual development and infection of host plants in M. oryzae.

• Journal of Microbiology
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• 제37권4호
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• pp.256-262
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• 1999

Latent membrane protein 1 (LMP1) of the Epstein-Barr virus (EBV) is an integral membrane protein with six transmembrane domains, which is essential for EBV-induced B cell transformation. LMP1 functions as a constitutively active tumor necrosis factor receptor (TNFR) like membrane receptor, whose signaling requires recruitment of TNFR-associated factors (TRAFs) and leads to NF-${\kappa}B$ activation. NF-${\kappa}B$ activation by LMP1 is critical for B cell transformation and has been linked to many phenotypic changes associated with EBV-induced B cell transformation. Deletion analysis has identified two NF-${\kappa}B$ activation regions in the carboxy terminal cytoplasmic domains of LMP1, termed CTAR1 (residues 194-232) and CTAR2 (351-386). The membrane proximal C-terminal domain was precisely mapped to a PXQXT motif (residues 204-208) involved in TRAF binding as well as NF-${\kappa}B$ activation. In this study, we dissected the CTAR2 region, which is the major NF-${\kappa}B$ signaling effector of LMP1, to determine a minimal functional sequence. A series of LMP1 mutant constructs systematically deleted for the CTAR2 region were prepared, and NF-${\kappa}B$ activation activity of these mutants were assessed by transiently expressing them in 293 cells and Jurkat T cells. The NF-${\kappa}B$ activation domain of CTAR2 appears to reside in a stretch of 6 amino acids (residues 379-384) at the end of the carboxy terminus.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제5권6호
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• pp.748-757
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• 1999

본 논문에서는 차량 영역의 추출을 이용한 효율적인 교통 혼잡도 측정 시스템을 설계하고 구현한다. 차량 영역 정보의 추출은 첫째 영역 분할, 둘째 작은 영역의 제거와 영역의 직사각형화, 셋째 영역의 병합 및 삭제의 단계로 나눌 수 있다. 영역 분할 단계에서는 획득한 도로 영상을 영역 기반 영역 분할에 의해 영역으로 분할한다. 그 다음 영역 분할 후의 영역 정보 중 차량 영역을 추출하는데 영향을 미치지 않는 작은 영역들을 제거하고, 남은 영역들을 직사각형화한다. 마지막으로 차선 별로 남은 영역들을 병합, 삭제함으로써 각 차선마다 차량 영역 정보를 추출할 수 있다. 이러한 방법은 배경 영상과 같은 부가적인 정보를 사용하지 않고 도로 자체 영상만으로 교통 혼잡도를 측정할 수 있으며, 그림자의 영향이 없을 경우 적용할 수 있는 기법이다.Abstract In this paper, we designed and implemented an efficient road congestion analysis system using regional information. To extract vehicle regions from a road image, the system process the image in five steps: segmentation, small region elimination, region rectangularization, region merging and region deletion. First, we segment road image by a threshold value. Then, we eliminate useless small regions to extract vehicle region, and perform region rectangularization. Finally, we extract vehicle region of each lane of the road by region merging and deletion. This method has the advantage of measuring road congestion without additional information such as background images. But this method must be applied to road images without shadow.

• Plant Breeding and Biotechnology
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• 제6권4호
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• pp.413-425
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• 2018

A previous work developed and identified a new omega-5 gliadin deficient wheat line named O-free by crossing Keumkang and Olgeuru, which is nutritionally quite meaningful in that omega-5 gliadin is one of the known wheat allergens. To verify the characteristics of the O-free, we performed RNA sequencing (RNAseq) analysis of the O-free and the two parent lines (Keumkang and Olgeuru). The results of the similarity analysis with the ESTs for gliadins and glutenins showed that the O-free ESTs had no similarity with the omega-5 gliadin sequences but had similarity to other gliadins and glutenins. Furthermore, mapping results between the raw RNAseq data from the O-free and the omega-5 gliadin sequence showed a clear deletion of the N-terminal sequences which are an important signature of omega-5 gliadin. We also designed specific PCR primers that could identify omega-5 gliadin in the genomic DNA. The results showed that no omega-5 gliadin fragments were detected in the O-free. According to these results, we confirmed that the deficiency of omega-5 gliadin in the O-free is not caused by post-transcriptional or post-translational regulations such as epigenetic phenomena but by a simple deletion in the chromosome. Furthermore, we showed that the low-molecular weight glutenin subunit (LMW-GS) gene in the O-free had a single nucleotide polymorphism (SNP) causing a premature stop codon, resulting in a truncated polypeptide. We expect that the O-free line may serve as an excellent source of wheat that could prevail in the hypo-allergen wheat market, which has recently gained interest world-wide.

• Journal of Genetic Medicine
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• 제15권2호
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• pp.64-71
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• 2018

Purpose: Overgrowth syndromes are conditions that involve generalized or localized areas of excess growth. In this study, the clinical, molecular, and genetic characteristics of Korean patients with overgrowth syndrome were analyzed. Materials and Methods: We recruited 13 patients who presented with overgrowth syndrome. All patients fulfilled inclusion criteria of overgrowth syndrome. Analysis of the clinical and molecular investigations of patients with overgrowth syndrome was performed retrospectively. Results: Among the 13 patients with overgrowth syndrome, 9 patients (69.2%) were found to have molecular and genetic causes. Among the seven patients with Sotos syndrome (SS), two had a 5q35microdeletion that was confirmed by fluorescent in situ hybridization. In two patients with SS, intragenic mutations including a novel mutation, c.5993T>A (p.M1998L), were found by Sanger sequencing. One patient had one copy deletion of NDS1 gene which was confirmed by multiplex ligation-dependent probe amplification. Among five patients with Beckwith-Wiedemann syndrome, three had aberrant imprinting control regions; 2 hypermethylation of the differentially methylated region of H19, 1 hypomethylation of the differentially methylated region of Kv. In one patient displaying overlapping clinical features of SS, a de novo heterozygous deletion in the chromosomal region 7q22.1-22.3 was found by single nucleotide polymorphism-based microarray. Conclusion: Considering high detection rate of molecular and genetic abnormalities in this study, rigorous investigations of overgrowth syndrome may be an important tool for the early diagnosis and genetic counseling. A detailed molecular analysis of the rearranged regions may supply the clues for the identification of genes involved in growth regulation.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2006년도 정기 학술대회 논문집
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• pp.514-519
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• 2006

This paper presents a new nonlinear analysis algorithm which uses the equivalent nodal load for the element stiffness. The equivalent nodal load represents the influence of the stiffness change such as the addition of elements, the deletion of elements, and/or the partial change of element stiffness. The nonlinear analysis of structures using the equivalent load improves the efficiency very much because the inverse of the structural stiffness matrix, which needs a large amount of computation to calculate, is reused in each loading step. In this paper, the concept of nonlinear analysis using the equivalent load for the element stiffness is described and some numerical examples are provided to verify it.

• 한국공간구조학회:학술대회논문집
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• 한국공간구조학회 2006년도 춘계 학술발표회 논문집 제3권1호(통권3호)
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• pp.170-174
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• 2006

This paper presents a new nonlinear analysis algorithm which uses the equivalent nodal load for the element stiffness. The equivalent nodal load represents the influence of the stiffness change such as the addition of elements, the deletion of elements, and/or the partial change of element stiffness. The nonlinear analysis of structures using the equivalent load improves the efficiency very much because the inverse of the structural stiffness matrix, which needs a large amount of computation to calculate, is reused in each loading step. In this paper, the concept of nonlinear analysis using the equivalent load for the element stiffness is described and some numerical examples are provided to verify it.

• 한국정보시스템학회지:정보시스템연구
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• 제23권3호
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• pp.127-142
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• 2014

Plagiarism occurs when the content is copied without permission or citation, and the problem of plagiarism has rapidly increased because of the digital era of resources available on the World Wide Web. An important task in plagiarism detection is measuring and determining similar text portions between a given pair of documents. One of the main difficulties of this task is that not all similar text fragments are examples of plagiarism, since thematic coincidences also tend to produce portions of similar text. In order to handle this problem, this paper proposed association analysis in data mining to detect plagiarism. This method is able to detect common actions performed by plagiarists such as word deletion, insertion and transposition, allowing to obtain plausible portions of plagiarized text. Experimental results employing an unsupervised document classification strategy showed that the proposed method outperformed traditionally used approaches.