• Title/Summary/Keyword: degree of branching

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Physicochemical Properties and Gel Forming Properties of Mungbean and Buckwheat Crude Starches (녹두와 메밀 조전분의 이화학적 특성 및 겔 형성)

  • 주난영;이혜수
    • Korean journal of food and cookery science
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    • v.5 no.2
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    • pp.1-8
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    • 1989
  • The physicochemical properties and gel forming properties of mungbean and buckwheat crude starches were investigated. The results were as follows; 1. The granule size and shape of mungbean crude starch were $11~32\mu\textrm{m}$ and oval, and those of buckwheat crude starch were $3~10 \mu\textrm{m}$ and polygonal. 2. The amylose conteut of mungbean crude starch and buckwheat crude starch were 78.0% and 26.4% respectively. 3. The blue value of mungbean crude starch and buckwheat crude starch were 1.030 and 0.409, respectively. 4. Periodate oxidation of mungbean crude starch resulted that amylose had the average molecular weight of 95, 648, degree of polymerization of 590 and amylopectin had the degree of branching of 5.4, glucose unit per segment of 18.6, and periodate oxidation of buckwheat crude starch resulted that amylose had the average molecular weight of 133, 690, degree of polymerization of 825 and amylopectin had the degree of branching of 5.2, glucose unit per segment of 19.2 5. Water binding capacity of mungbean crude starch was 185.1% and that of buckwheat was 209.9% 6. The pattern of change in swelling power of mungbean crude starch for increasing temperature started to increase at $60^{\circ}C$ and increased rapidly from $70^{\circ}C$, and that of buckwheat increased slowly from $60^{\circ}C$ to $90^{\circ}C$ without rapid increase. 7 The ranges of gelatinization temp. of mungbean and buckwheat crude starches were 63. 9-$109^{\circ}C$ and 52.5-84.$2^{\circ}C$, respectively. 8. The gelatinization patterns for 6% munbean crude starch and 8% buckwheat crude starch were investigated by Brabender amglograph. Mungbean crude starch showed the initial pasting temperature of 77.6$^{\circ}C$ without peak height, and buckwheat crude starch showed that of $62.5^{\circ}C$ without peak height. In addition, sensory evaluation for sample starch gels (mungbean, buckwheat, cowpea) was done. 1. The difference of sensory characteristics for each starch gel was significant. 2. The sample starch gels were regarded as 'Mook' by pannels. 3. 74.44% of the degree of Mooklike was explained by hardness.

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Functional Haplotypes and Evolutionary Analyses of SBE1 in Collected Rice Germplasm

  • Thant Zin Maung;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.216-216
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    • 2022
  • The starch-branching enzymes (BEs) are responsible for synthesizing the amylopectin, which plays an important role in determining the structural and physical properties of starch granules. BE has two differently functioning isoforms (BEI and BEIIa/b) based on their difference in the chain-length pattern by the degree of polymerization (DP), which mainly contributes to the amylopectin chain length distribution in starch biosynthesis. In this study, we investigated functional haplotypes and evolutionary analyses of SBE1 in 374 rice accessions (320 Korean bred and 54 wild). The analyses were performed based on the classified subpopulations. Haplotype analysis generates a total of 8 haplotypes, of which only four haplotypes were functional carrying four functional SNPs in four different exons of SBE1 on chromosome 6. Nucleotide diversity analysis showed a highest pi-value in aromatic group (0.0029), while the lowest diversity value was in temperate japonica (0.0002), indicating the signal of this gene evolution origin. Different directional selections could be estimated by negative Tajima's D value of temperate japonica (-1.1285) and positive Tajima's D value of tropical japonica (0.9456), where the selective sweeps were undergone by both positive purifying and balancing selections. Phylogenetic analysis indicates a closer relationship of the wild with most of the cultivated subgroups indicating a common ancestor for SBE1 gene. FST-values indicate distant genetic relationships of temperate japonica from all other classified groups. PCA and population structure analysis show an admixed structure of wild and cultivated subpopulations in some proportions.

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A Review of Ocean Circulation of the East/Japan Sea (한국 동해 해수순환의 개략적 고찰)

  • 김종규
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2001.10a
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    • pp.103-107
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    • 2001
  • The major studies of an ocean circulation of the East/Japan Sea related to evaluate the feasibility and utilization of deep ocean water are reviewed. The major feature of surface current system of the East/Japan Sea is an inflow of the Tsushima Warm Current through the Korea/Tsushima Strait and the outflow through the Tsugaru and Soya Straits. The Tsushima Warm Current has been known to split into two or three branches in the southern region of the East/Japan Sea. In the cold water region of the East/Japan Sea, the North Korean Cold Current turns to the east near 39$^{\circ}$N after meeting the East Korean Warm Current, then flows eastward. The degree of penetration depends on the strength of the positive wind stress curl, according to the ventilation theory. Various current meter moorings indicate strong and oscillatory deep currents in various parts of the basin. According to some numerical experiments, these currents may be induced by pressure-topography or eddy-topography interaction. However, more investigations are needed to explain clearly the presence of these strong bottom currents. This study concludes the importance of topographical coupling, isopycnal outcropping, different wind forcing and the branching of the Tsushima Warm Current on the circulation of the East/Japan Sea.

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Physico Chemical Properties of Korean Ginseng (Panax ginseng C.A. Meyer) Root Starch II. Chemical Properties of the Starch (고려인삼 ( Panax ginseng. C.A. Meyer)전분의 이화학적 특성에 관한 연구 제 2보 전분의 화학적 특성)

  • Kim, Hae-Jung;Jo, Jae-Seon;Yu, Yeong-Jin
    • Journal of Ginseng Research
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    • v.8 no.2
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    • pp.124-134
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    • 1984
  • Ginseng root starch, prepared by conventional method, contained crude lipid of 0.5%, crude protein of 0.4%, crude mineral of 0.17% and phosphorous of 12.5mg% as noncarbohydrate constituents. The amylose content of ginseng root starch picked in Summer (May to August) and Winter (November to March) was 32-35% and 15-20%, respectively, and it was decreased with a growing preiod of ginseng. The blue value, alkali number and ferricyanide number of the starch were 0.14-0.17, 8.50 and 0.781, respectively. The molecular weight of amylose in the starch was estimated to be 1.27-7.95${\times}$105 by means of periodate oxidation, and the degree of branching and glucose unit per segment of amylopectin were 3.50-3.53% and 28.3-28.5, respectively, The starch content of ginseng root was decreased when dried under sunlight and stored at 5$^{\circ}C$ for twenty days. In contrast, sucrose content in the root was increased from 3.8% in fresh state to 11.5% during storage at the above condition. In the other hand, starch was converted to maltose by heating at temperature above 70$^{\circ}C$.

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Modeling of Rheological Properties of Pectins by Side Branches (펙틴의 곁사슬에 의한 유변학적 성질에 대한 모델)

  • Shin, Hae-Hun;Hwang, Jae-Kwan
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.583-589
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    • 2002
  • The rheological properties of apple pectins and tomato pectins with different degrees of side branches (sample I and sample II) were investigated with wide range of shear rate by theological modeling. Among the Power law model, Cross model and Carreau model, the Carreau model was the best fitted to the experimental data. Increasing in branching of apple pectins resulted in higher zero-shear viscosity $({\eta}_0)$. But, tomato pectins that have a low degree of side branches were shown litte difference between sample I and sample II. It is concluded that side branches of pectins can result in significant difference in rheological properties. And, this is predicted by the Carreau model.

Immune Stimulating Efficacy of Insoluble $\beta$-l, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP (Agrobacterium sp. R259 KCTC 10197BP로부터 생산된 $\beta$-1, 3-glucan의 면역 활성 효능)

  • 심정현;최원아;상병찬;윤도영
    • YAKHAK HOEJI
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    • v.46 no.6
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    • pp.459-465
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    • 2002
  • $\beta$-l, 3-glucans are well known to enhance the immune reactions, resulting in antitumor, antibacterial, antiviral, anticoagulatory and wound healing activities. $\beta$-1, 3-glucans have various activities depending on molecular weight, degree of branching, conformation, water-solubility and intermolecular association. However, the $\beta$-1, 3-glucans linked backbone structure is essential and $\beta$-D-glucopyranosyl units are required for immunopotentiating activities. In this study, we tested the immunophamacological activities of insoluble $\beta$-1, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP and confirmed the following activities: (1) IFN-${\gamma}$ production in PBMCs in the presence or in the absence of PHA, LPS, IL-18, and IL-12; (2) the induction of various cytokines in the spleen and thymus; (3) the adjuvant effect on the antibody production; (4) the cytotoxic and antitumor effects on cell lines and ICR mice. These results strongly suggest that $\beta$-1, 3-glucan from Agrobacterium sp. R259 KCTC 10197BP possesses various immunopharmacologica1 activities.

Ceramium riosmenae sp. nov. (Ceramiaceae, Rhodophyta): a new complete corticated species on Gracilaria from Baja California Sur, Mexico

  • Won, Boo-Yeon;Cho, Tae-Oh
    • ALGAE
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    • v.26 no.4
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    • pp.289-297
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    • 2011
  • Ceramium riosmenae sp. nov. is described from Baja California Sur, Mexico based on morphological and molecular data. The new species is characterized by erect thalli only, penetrating rhizoids on Gracilaria, 7-8 periaxial cells, five cortical initials per periaxial cell, complete cortication throughout, an average of 11-12 segments between branching points, rare adventitious branchlets, and cruciate tetrasporangia. Although C. riosmenae sp. nov. is similar to C. interruptum, C. sinicola, and C. codicola reported from Baja California Sur, Mexico in size and habit, it differs from these species in the number of cortical initials, habit, degree of cortication, host, and the shape of rhizoidal tips. C. riosmenae is separated from C. interruptum with interrupted cortication and four cortical initials from C. sinicola with spins near the apex and incomplete cortication near the base and from C. codicola with bulbous rhizoids on Codium. Our rbcL sequences reveal sufficient sequence divergence (2.4-3.9%) between C. riosmenae and C. interruptum, C. sinicola, and C. codicola to warrant species recognition and to separate C. riosmenae from these species on a phylogenetic tree.

Synthesis of High Molecular Weight Poly(vinyl alcohol) by Low Temperature Polymerization of Vinyl Acetate in Tertiary Butyl Alcohol and the Following Saponification (아세트산비닐의 삼차부틸알코올계 저온 중합 및 비누화에 의한 고분자량 폴리비닐알코올의 합성)

  • 류원석;한성수;최진현;유상우;홍성일
    • Polymer(Korea)
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    • v.24 no.5
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    • pp.610-620
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    • 2000
  • Vinyl acetate (VAc) was polymerized at 30, 40, and 5$0^{\circ}C$ using 2,2'-azobis (2,4-dimethylvaleronitrile) (ADMVN) and tertiary butyl alcohol (TBA) as the initiator and the solvent, respectively. High molecular weight (HMW) atactic poly(vinyl alcohol) (PVA) was prepared by saponifying the poly(vinyl acetate) (PVAc) synthesized. The effect of polymerization conditions were investigated in terms of conversion, degree of branching for acetyl group of PVAc, and molecular weight of both PVAc and PVA. The polymerization rate of VAc in TBA was proportional to the 0.49th power of ADMVN concentration in good accordance with the theoretical value of 0.5. HMW-PVA with high yield could be obtained successfully, probably due to lower polymerization temperature and decreased chain transfer reaction rate which was achieved by adopting ADMVN and TBA. PYAc having average degree of polymerization (P$_{n}$) of 10000~13000 was obtained at the conversion of 35~70%. Saponification of so prepared PVAc yielded PVA having P$_{n}$ of 2400~6100. The syndiotactic diad content increased with decreasing polymerization temperature and increasing VAc concentration due to a steric hindrance effect of TBA during polymerization.

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Growth Habit and Morphological Characteristics of Fasciated Soybean, var. Buchai-kong (Glycine max L. Merrill) (대화형대두 부채콩의 생육 및 형태적 특성)

  • ;Jong-Kyu Hwang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.167-172
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    • 1986
  • This experiment was carried out to obtain some information about the growth habit and morphological and anatomical characteristics of fasciated soybean, var. Buchai-kong. The results obtained are summarized as follows: 1. Main stern of fasciated soybean, var. Buchai-kong was gradually broadened and became flat, on which leaves emerged irregularly compared with normal soybean, var. Danyeop-kong. The number of leaves on main stern was more in Buchai-kong, but the total number of leaves per plant was not considerably different each other. 2. The degree of fasciation (max./min. in diameter at the broadened part) in main stern of Buchai-kong was about 3.1, and was related to the amount of growth of main stern and branches of Danyeop-kong. Stern fasciation types found in Buchai-kong were linear (the most common) and bifurcated with regard to branching of main stern tip. 3. The number of branches per plant was much less in Buchai-kong than in Danyeop-kong. There were fas-ciated branches and fasciated petiole in Buchai-kong. 4. About 75 percent of pods of Buchai-kong were concentrated on the top of stern, which showed strong pod clustering. 5. Seed yield per plant of Buchai-kong was about 60 percent of that of Danyeop-kong, and the decrease in seed yield was mostly affected by the decrease in seed weight and seeds per pod. 6. The number of vascular bundles of transection of mature stern was more in Buchai-kong, but Danyeop-kong developed the better vascular bundle.

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High-Level Production of Low-Branched Levan from Pseudomonas aurantiaca S-4380 for the Production of $di-\beta-D-Fructofuranose$ Dianhydride IV

  • JANG KI-HYO;JANG EUN-KYUNG;KIM SEUNG-HWAN;KIM IN-HWAN;KANG SOON AH;KOH ISSAC;PARK YOUNG-IL;KIM YOUNG-JUN;HA SANG-DO;KIM CHUL HO
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.102-108
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    • 2006
  • The IscA gene, encoding a levansucrase of 424 amino acids (aa) residues, was cloned from the genomic DNA of Pseudomonas aurantiaca S-4380, and overexpressed in Escherichia coli. The recombinant levansucrase overexpressed in E. coli was then used to produce levan from sucrose. Levan crystals with 98% purity could be obtained from the reaction mixture with $62\%$ yield using an alcohol precipitation method. The molecular weight of the levan was $7\times10^5$ daltons. Methylation studies showed that the levan was branched: main linkage C-2,6; branched linkage C-2,1; and degree of branching $6\%$. Three bacterial levans from different strains were incubated with levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032, which produced $di-\beta-D-fructofuranose$ dianhydride IV (DFA IV); final conversion yields from the levans to DFA IV were $39\%$ in Zymomonas mobilis, $53\%$ in Serratia levanicum, and $59\%$ in P. aurantiaca S-4380 levansucrase. The levan from P. aurantiaca S-4380 levansucrase gave the highest conversion yield of levan to DFAIV so far reported.