• BMB Reports
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• v.48 no.8
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• pp.431-437
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• 2015

Notch signaling plays a pivotal role in cell fate determination, cellular development, cellular self-renewal, tumor progression, and has been linked to developmental disorders and carcinogenesis. Notch1 is activated through interactions with the ligands of neighboring cells, and acts as a transcriptional activator in the nucleus. The Notch1 intracellular domain (Notch1-IC) regulates the expression of target genes related to tumor development and progression. The Notch1 protein undergoes modification after translation by posttranslational modification enzymes. Phosphorylation modification is critical for enzymatic activation, complex formation, degradation, and subcellular localization. According to the nuclear cycle, Notch1-IC is degraded by E3 ligase, FBW7 in the nucleus via phosphorylation-dependent degradation. Here, we summarize the Notch signaling pathway, and resolve to understand the role of phosphorylation in the regulation of Notch signaling as well as to understand its relation to cancer. [BMB Reports 2015; 48(8): 431-437]

• Journal of Korean Society of Environmental Engineers
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• v.28 no.7
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• pp.713-720
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• 2006

To analyze atmospheric fate of PCBs in the Kanto region, Japan, an one-compartment box model was used and the relationship between behavior of each PCB homologue and air temperature was simulated. In addition, the emission rates and the deposition fluxes in the overall Kanto region were estimated by the model. The total emission rate and deposition flux was 3,320 kg/yr and 1,480 kg/yr, respectively. The contribution of advection was ranged from 22 to 38% among elimination processes(advection, dry & wet deposition and degradation) of PCBs from atmosphere. The rates of degradation(OH radical process) for PCBs in the Kanto region would be negligible. This study showed that one-compartment box model can be available to understand the overall atmospheric behavior of PCBs.

• Molecules and Cells
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• v.21 no.2
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• pp.218-223
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• 2006

The effect of poly(ADP-ribosyl)ation on the stability of p53 in SK-HEP1 cells treated with UV light was examined. Intracellular levels of p53 increased in cells treated with a low dose of UV light ($20J/m^2$), whereas they increased but then declined after a higher dose of UV ($100J/m^2$). Intracellular levels of p53 in the UV treated SK-HEP1 cells were dependent on the UV dose. Use of proteasome inhibitors revealed that p53 is degraded by proteasomal proteolysis after high doses of UV light. We present evidence that, at low doses, poly(ADP-ribose)polymerase (PARP) poly(ADP-ribosyl) ates p53 and protects it from proteasomal degradation before caspase-3 is activated, whereas at high doses the cells undergo UV induced apoptosis and PARP is cleaved by caspase-3 before it can protect p53 from degradation. Destabilization of p53 by cleavage of PARP may be important in cell fate decision favoring apoptosis.

• Development and Reproduction
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• v.15 no.3
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• pp.215-221
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• 2011

Endocytosis of the Notch ligand, DeltaD, by mind bomb1 is indispensable for activation of Notch in cell fate determination, proliferation, and differentiation during zebrafish neurogenesis. Loss of mind bomb1 activity as an E3 Ubiquitin ligase causes the accumulation of deltaD at the plasma membrane and results in the ectopic neurogenic phenotype by activation of Notch in early zebrafish embryogenesis. However, the regulatory mechanism of deltaD during neurogenesis is not identified yet. This study aims to analyze the pathway of mib1 and deltaD after endocytosis in vivo during zebrafish embryogenesis. Mind bomb1 and deltaD are co-localized into autophagosome and mutant form of mind bomb1 fails to cargo deltaD into autophagosomes. These findings suggest that mind bomb I mediates deltaD regulation by autophagy in an ubiquitin-dependent manner during zebrafish embryogenesis.

• Proceedings of the Korean Geotechical Society Conference
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• 2007.09a
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• pp.542-557
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• 2007

Contamination of subsurface results in degradation of geomaterials (i.e., soils and rock mass), in the long run. This is mainly due to the presence of chemical and/or radiological materials in undesirable concentrations and at elevated temperatures. However, as contaminant-geomaterial interaction is an extremely slow and complex process, which primarily depends on their physical, chemical and mineralogical properties, it is quite difficult to study this interaction under laboratory or in situ conditions. In such a situation, accelerated physical modeling, using a geotechnical centrifuge, and finite element/difference based numerical modeling techniques are found to be quite useful. This paper presents details of various modeling techniques developed by the researchers at the Indian Institute of Technology Bombay, Mumbai, India, for studying heat migration, flow and interaction (fate) of reactive and non-reactive contaminants in the geoenvironment, under saturated and unsaturated conditions. In addition, paper presents details of the technique that can be employed for determining susceptibility of a material to undergo physico-chemico-mineralogical alterations due to its interaction with contaminants.

• Journal of Environmental Science International
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• v.17 no.3
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• pp.343-350
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• 2008

The fate of two cyclic ethers, THF(Tetrahydrofuran) and 1,4-Dioxane, in conventional biological wastewater treatment plants was investigated using sequential activated sludge process. Removal efficiency of THF were about 86% in average, which was greater than that of 1,4-Dioxane, 30%. However, it was not clear whether the removal of cyclic ethers in biological system was caused by microbial activity or not. Thus treatability tests were conducted by batch experiments. The effects of mixing, aeration and the addition of activated sludge on the removal of cyclic ethers were investigated in batch experiments. THF was totally removed by mixing and aeration in 24 hours while removal ratio of 1,4-Dioxane was at most 30% for the same period. This results could be ascribed to the differences in Henry's law constants between the two chemicals. In addition, biological degradation including biosorption was not obviously observed in these batch tests.

• BMB Reports
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• v.42 no.3
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• pp.131-135
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• 2009

MicroRNAs control gene expression by inhibiting translation or promoting degradation of their target mRNAs. Since the discovery of the first microRNAs, lin-4 and let-7, in C. elegans, hundreds of microRNAs have been identified as key regulators of cell fate determination, lifespan, and cancer in species ranging from plants to humans. However, while microRNAs have been shown to be particularly abundant in the brain, their role in the development and activity of the nervous system is still largely unknown. In this review, we describe recent advances in our understanding of microRNA function at synapses, the specialized structures required for communication between neurons and their targets. We also propose how these advances might inform the molecular model of memory.

• Journal of Pharmaceutical Investigation
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• v.22 no.1
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• pp.1-10
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• 1992

The use of erythrocyte as drug carrier has been reviewed, Carrier erythrocytes have proven to offer many advantages for delivery of therapeutic agents, especially in the treatment of inherited enzyme deficiency and cancer. Carrier erythrocytes are biodegradable and nonimmunogenic. Encapsulated drugs may be protected from premature degradation, inactivation and excretion. Carrier erythrocytes may be used as a slow-release system. Targeting of encapsulated drugs directly to a site of action is another possibility. Methods for encapsulating drugs into erythrocytes, the fate of carrier erythrocytes in vivo, the strategies of targeting carrier erythrocytes to special organs and in vivo applications of erythrocytes have been discussed. The encapsulation of drugs in erythrocytes has shown attractive possibilites in future use.

• Bulletin of the Korean Chemical Society
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• v.23 no.3
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• pp.432-436
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• 2002

Benomyl, one of the known endocrine disrupting chemicals, was analyzed to understand the fate in the nature. Water, sediment and biota samples are acidified to hydrolyze benomyl into carbendazim then followed by extraction and concentration. The concentrations of carbendazim in the samples were determined by liquid chromatography/time-of-flight mass spectrometer. Analysis data showed that certain amount of carbendazim was accumulated in sediment. On the contrary, no sign of accumulation in biota was observed probably due to the increased degradation rate in vivo. It is, however, that no one can claim carbendazim is not harmful to biota, since carbendazim may give a negative effect against organisms at the point of intaking.

• Korean Journal of Environmental Agriculture
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• v.26 no.4
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• pp.351-363
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• 2007

The scientific articles using radio labelled insecticides performed by Korean researchers were reviewed. The research works were divided into 4 categories such as soil, plant, animal and insect. All researches used $^{14}C$-labelled chemicals, and the $^{14}C$-carbofuran was widely used among them. Fate of insecticides, bound-residues and metabolic process were staple concerning area in soil study. And the uptake and translocation, metabolism and metabolites also a major interests in plant study. As well as the degradation, metabolic pathway and metabolites, and distribution of chemicals in animal tissue were another point of consideration in animal study. And finally, the penetration ratio into body and resistant mechanism were the major concerning views of study with insects.