• Title/Summary/Keyword: decay fungi

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Occurrence and Biological Control of Postharvest Decay in Onion Caused by Fungi

  • Lee, Joon-Taek;Bae, Dong-Won;Park, Seun-Hee;Shim, Chang-Ki;Kwak, Youn-Sig;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.17 no.3
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    • pp.141-148
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    • 2001
  • Postharvest decay of onion bulbs was examined by inspecting the commercial packages in the market or in storage. Bulb rot incidence was unexpectedly high, and onion bulbs with 1st quality grade were rotten most severely by 51%, followed by 32% for 2nd and 21% for 3rd grades. This indicates that larger bulbs had higher incidences of bulb rots. Major pathogens associated with basal and neck rots were Fusarium oxysporum and Aspergillus sp. or Botrytis allii, respectively, of which basal rot was most prevalent and damaging during storage. Among the epiphytic microorgani는 from onion plants, several Bacillus and Paenibacillus spp. and previously selected Pseudomonas putida and Trichoderma harzianum had inhibitory efficacy against bulb rot pathogens. Among these B. amyloliquefaciens BL-3, Paenibacillus polymyxa BL-4, and P. putida Cha 94 were highly inhibitory to conidial germination of F. oxysporum and B. allii. P. putida Cha 94, B. amyloliquefaciens BL-3, P. polymyxa BL-4, and T. harzianum TM were applied in the rhizoplane of onion at transplanting. Initially antagonist populations decreased rapidly during the first one month. However, among these antagonists, rhizoplane population densities of BL-3, Cha 94, and TM were consistently high thereafter, maintaining about 10$^4$-10$^{5}$ cells or spores per gram of onion root up to harvest time. The other bacterial antagonist BL-4 survived only for two months. TM was the most effective biocontrol agent against basal rot, with the number of rotten bulbs recorded at 4%, while that of the control was 16%. Cha 94 was effective for the first 20 days, but basal rot increased thereafter and had about the same control efficacy as that of BL-3 and BL-4. When the antagonists were applied to the topping areas of onion bulbs at harvest, TM was the most effective in protecting the stored onion bulbs from neck rotting. The second effective antagonist was BL-3. TM and BL-3 completely suppressed the neck rot in another test, suggesting that biocontrol of postharvest decay of onion using these microorganisms either at the time of transplanting or at harvesting may be promising.

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Soil Environment and Soil-borne Plant Pathogen Causing Root Rot Disease of Ginseng (인삼 뿌리썩음병 발병에 미치는 토양전염성병원균과 토양환경요인)

  • Shin, Ji-Hoon;Yun, Byung-Dae;Kim, Hye-Jin;Kim, Si-Ju;Chung, Doug-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.3
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    • pp.370-376
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    • 2012
  • Disease is the major problem in ginseng cultivation from seed stratification, soil preparation prior to planting, right through to drying of the roots. There are many soil-borne disease pathogen in rhizosphere soil environment, furthermore occurrence of diseases by a diverse group of fungi and related organisms are closely related to various soil condition. Observable symptoms for soil-borne diseases include wilting, leaf death and leaf fall, death of branches and limbs and in severe cases death of the whole plant. The fungus Cylindrocarpon destructans is the cause of root rot characterized by a decay of the true root system in many ginseng production areas in Korea. Some pathogens are generally confined to the juvenile roots whilst others are capable of attacking older parts of the root system. However, the relation between the soil environmental characteristics and ginseng root rot by soil-borne disease pathogen is not clearly identified in ginseng field. In this paper, we reviewed soil-borne plant pathogen causing root rot disease of ginseng with respect to soil environment.

Evaluation of Fungicidal, Anti-sapstain and Termiticidal Efficacy of Dan-Chung Treated Blocks (단청처리재의 방부.방미.방의(흰개미)효력 평가)

  • 이명재;이동흡;손동원
    • Journal of Korea Foresty Energy
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    • v.22 no.2
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    • pp.36-43
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    • 2003
  • This study was carried out to evaluate the fungicidal, anti-sapstain and termiticidal efficacy of commercial Dan-Chung which are currently being used for painting to decorate and to provide durability with structures of temples. Two binders (Polysol and Gelatine), and eleven pigments (Iron Oxide Red, Lead Red, Toluidine Red, Chrome Yellow, Titanium Dioxide, Ultramarine Blue, Chrome Oxide Green, Permanent Black, Cyanine Green and Ho-bun(Chalk)) were applied for the evaluation. The fungicidal and anti-sapstain activity of each pigment were depended on the types of binders. When the Polysol was used as a binder, the fungicidal efficacy of Toluidine Red treated specimen was close to the standard value (above 80) with grater than 70 against to two wood decay fungi (T. palustris and T. versicolor). The anti-sapstain efficacy of Lead Red treated specimens was excellent against to three sapstain fungi (P. funiculosum, T. viride and R. nigricans), and that of Ultramarine Blue treated specimens was also excellent to T. viride. The other pigments had little efficacy to these micro-organisms. Although there were some different trends of the effectiveness depending on the types of binders, the anti-termite effectiveness of Chrome Oxide Green and Chrome Yellow was outstanding to termite tests.

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Preservatory effect of stored 'Setoka'(Citrus sp.) using the noncontacted low temperature atmospheric pressure surface discharged plasma (비접촉식 저온 대기압 면방전 플라즈마를 이용한 저장 '세토카' 감귤의 선도유지 효과)

  • An, Hyun Joo;Park, Kyung Jin;Kim, Sang Suk
    • Food Science and Preservation
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    • v.23 no.6
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    • pp.772-777
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    • 2016
  • Activity of the noncontacted low temperature atmospheric pressure surface discharged plasma (LASDP) converts stable gas to ionized gas known as discharge or plasma. This ionized gas exhibits the antimicrobial activity. We examined the effects of 3 different storage treatments for 80 days on 'Setoka' : ambient storage (AS), low-tempperature storage (LTS), and low-temperature atmospheric pressure plasma+low-tempperature storage (PLTS). Total soluble solids showed no the significant differences between the 3 treatments. Acidity gradually decreased, and was 0.5% under AS after 30 days of storage. Fruit firmness increased by a few percent until 40 days of storage. Weight loss in AS was higher than for other treatments. After 80 days of storage, the decay ratio was significantly low in PLTS treatment: (AS, 50.5%; LTS, 5.6%; PLTS, 1.9%). In AS treatment, 73% of the rotten fruits were infected particularly with green and blue mold; however, only 1% of the rotten fruits were infected in case of PLTS treatment. In conclusion, LASDP treatment can prevent postharvest decay caused by fungi and is an efficacious alternative extending the shelf-life of citrus fruits.

Efficacy of Wood Preservatives Formulated with Okara and Its Microscopic Analysis (두부비지 방부제의 방부효능 및 현미경적 분석)

  • Kim, Ho-Yong;Choi, In-Gyu;Ahn, Sye Hee;Oh, Sei Chang;Youn, Young Ho;Yang, In
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.3
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    • pp.245-254
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    • 2009
  • As a substitute for CCA, which is inhibited due to its environmental pollution and human harmfulness, and CuAz and ACQ with a high cost, okara-based wood preservatives were formulated with okara hydrolyzates using copper sulfate and/or borax as a metal salt. The efficacy of the preservatives and X-ray microanalysis of wood specimens treated with the preservatives were examined to confirm the potential of the okara-based wood preservatives. Most of the preservatives showed excellent decay resistance against brown-rot fungi, Postia placenta and Gloeophyllum trabeum. The efficacy was improved when the acid concentration and temperature used for the hydrolysis of okara increased. In addition, when borax was added into copper sulfate/okara hydrolyzates preservative formulations, any decay was not found in the specimens. From the microscopic observation of the specimens treated with okara-based wood preservatives, it seems that okara is contributed to the fixing of metal salts in wood blocks. Therefore, it is speculated that okara-based wood preservatives can effectively protect wood against fungal attack as CuAz, and that the preservatives are sufficient to use as an alternative wood preservative of CCA, ACQ and CuAz.

Penetration of ACQ Treatment and its Effect of Degradation on Wood Tissues (Structure) (ACQ 약제의 목재 내 침투 및 부후 특성에 미치는 목재 조직의 영향)

  • Lee, Hyun-Mi;Lee, Dong-Heub;Hwang, Won-Joung
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.6
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    • pp.576-582
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    • 2013
  • To obtain the basic information on wood preservative process using copper, the penetration ACQ-2 preservative agent into Douglas fir wood and its degradational characteristics were investigated. Douglas fir is known to be difficult for preservative agent injection. To find the hindrance factor of the preservatives movement, the ray height, ray density, and length of tracheid were measured and observed by a stereoscopic microscope and scanning electron microscope. The results were compared in the well-penetrated parts of preservatives and insufficient parts. There were no significant differences in height, length, and density between both parts, except for the pits of the wood elements observed in SEM micrograph. More declosed pits were found in the well-penetrated parts of preservatives. Decay characteristics of wood specimen treated with various ACQ concentrations by brown-rot fungi was observed by an optical microscope. The decayed properties of tracheid, rays, and resin canals was found in the non-treated wood specimens and it was also found that some extent of preservatives concentration is necessary to protect the decay.

Consideration of Daldinia childiae as a New Record in Korea, Based on Morphological Characteristics of Korea Collections (한국에서 발견된 국내 미기록종 Daldinia childiae의 미세형태적 고찰)

  • Velmurugan, Natarajan;Han, Sang-Sub;Sa, Dong-Min;Lee, Yang-Soo
    • Applied Microscopy
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    • v.37 no.4
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    • pp.289-295
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    • 2007
  • The genus Daldinia is a member of the Xylariaceae that has brown to dark brown and phaseoliform single cell ascospores with a conspicuous full germ slit. The isolate of D. childiae collected from Mt. Deuk-yu in Korea were compared with similar taxa, D. eschscholzii and D. concentrica.. Ascospores were $11{\sim}13{\mu}m{\times}5.5{\sim}6{\mu}m$ in size, light brown to brown, unicellular, ellipsoid-inequilateral, with dull round ends. Ascospore showed very faint ornamentation at ${\times}7.0k$ magnification. It is one of the main morphological characteristic Korean collection of D. childiae under SEM level and a main reason of reinterpretation of D. concentrica in Korea. Using KOH-extractable pigment color of stroma, D. concentrica that also has supported the SEM level investigation. Daldinia concentrica, having those characteristic recorded in Korea, should be renamed as D. childiae. However, the isolate will be the first record as D. childiae, having precise morphological description in Korea.

Germ Tube Formation of Ascospores of Two Terrestrial Higher Ascomycetes, Hypoxylon mammatum and H. truncatum

  • Lee, Yang-Soo;Han, Sang-Sub;Shin, Jong-Ho;Lee, Young-Mi;Song, Bong-Keun
    • Journal of the Korean Wood Science and Technology
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    • v.28 no.4
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    • pp.10-16
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    • 2000
  • Two wood decay ascomycetes fungi identified as Hypoxylon mammatum and H. truncatum were isolated from backyard of Korea Research Institute of Chemical Technology (KRICT) in Korea. Hypoxylon truncatum is newly recorded as a wood degrader in Korea. Unusual germination mechanisms of ascospores in H. mammatum and H. truncatum are described and illustrated. The differences between two species were noticed on the process of germ tube formation. In the process of germ tube formation, the fast movement to pigmented ascospores activated from their perispores was termed as spore eclosion that was only found in H. mammatum. This sophisticated recognition mechanism indicated the existence of specific eclosion and germ tube formation due to the composition of cell wall layers and their preferable host derive, based on examined two species under a genus. The observation on present study postulates different composition of wall layers of ascospore and different nutrient composition for germination.

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evelopment of biological methods for improving the storage qualities of sweet persimmon harvested in Gyeongsangnam-do (생물학적 처리방법에 의한 경남산 단감의 저장성 향상을 위한 기술개발)

  • 조성환;김영록
    • Journal of Food Hygiene and Safety
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    • v.10 no.4
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    • pp.263-270
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    • 1995
  • In order to promote the prevention of microbial and enzymatic spoilage and to retain the freshiness, sweet persimmons harvested in Gyeongsangnam-do were treated with graperfruit seed extract(GFSE)-CaCO3 mixture and stored in the proper packaging conditions. A low concentration of GFSE showed effective growth inhibition of plant pathological bacteria and fungi, Enterobacter pyrinus and Fusarium sp., which were involved in the decay of fruits and vegetables. GFSE was stable to heat treatment; its antimicrobial activity was not changed by heat treatment upto 10$0^{\circ}C$. However, when the temperature was raised to 12$0^{\circ}C$, about 90% of total activity was retained within 30 min. GFSE was also highly stable to broad pH changes; its activity was not changed in the range of pH 2.0 to pH 12.0. The physiological function of cell membrane in the spores of Bacillus cereus and the hyphae of Fusarium sp. was destroyed by treating with GFSE. It was observed that treating sweet persimmons with GFSE minxture and storing them in strech-wrapped packages could prolong the greshness of sweet persimmons and reduce quality deterioration.

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Two Unrecorded Wood Decay Fungi (Polyporales) in Dadohaehaesang National Park, Korea

  • Park, Myung Soo;Kim, Nam Kyu;Kim, Min-Ji;Park, Jae Young;Kim, Taeheon;Park, Jung-Won;Lim, Young Woon
    • The Korean Journal of Mycology
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    • v.46 no.1
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    • pp.43-50
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    • 2018
  • Dadohaehaesang National Park is a well-known habitat for a variety of indigenous animals and plants; however, there are no studies on the diversity of macrofungi. To undertake a basic survey of the macrofungi diversity in an unexploited area, we focused on surveying the macrofungi in Dadohaehaesang National Park, Korea, during 2017 and discovered two unrecorded species belonging to the order Polyporales. The two species were identified as Cinereomyces lindbladii and Steccherinum bourdotii based on a combination of morphological characteristics and sequence analysis of the internal transcribed spacer region. We provide macro- and micro-morphological descriptions for these two unrecorded species in Korea.