• Journal of Chest Surgery
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• v.17 no.1
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• pp.157-166
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• 1984

These biologic test procedures are designed to test the suitability of P.V.C. made in Korea intended for parenteral preparation, which were based on the U.S. Pharmacopeia XIX "Biologic Test-Plastic Container", Official from July 1, 1975. Healthy adult human blood and rabbits weighing 2\ulcorner.2Kg were used for test materials. Sample P.V.C. were sampled from the medical equipments made in Korea randomly and Control P.V.C. were sampled from the standardized Cobe and Polystan P.V.C. tubes. P.V.C. extract was prepared from a homogeneous P.V.C. samples by incubating 60 square centimeters of the sample per 20 millimeters of sterile pyrogen-free saline at 70\ulcorner for 72 hours or autoclaving at 120\ulcorner for 1 hour. The Implantation Test was designed to evaluate the reaction of living tissue to the plastic by the method of the implantation of the Sample itself into animal tissue. The Systemic Injection Test, the Intracutaneous Test, and the remainders were designed to determine the biological response of animals to plastics by the single-dose injection of specific extracts prepared from a Sample. The results are as follows; 1.Implantation Test - No significant difference for reactions was noted between the Sample treated animal and the Control after 72 hours of implantation. 2.Systemic Toxicity Injection Test - No sign of toxicity and/or death immediately after injection and at 4, 24, 48 hours respectfully after injection. 3.Intracutaneous Test - None of the animals treated with the Sample showed a significantly greater reaction than the observed in the animals treated with Blank. 4.Pyrogen Assay-Only one animal treated with the Sample showed the maximal rise of rectal temperature about 0.2\ulcorner after 3 hours of injection, but remainders showed no change. 5.Hemolytic Index - The positive Control tube of distilled water exhibited complete hemolysis while the negative Control tube and P.V.C. extract were negative demonstrating no hemolysis. 6.Cell Morphology of Erythrocytes and Leukocytes on Stored, Heparinized Human Blood -- There was no significant difference in the morphology of either the Control or Sample extract. 7.Clotting Mechanism of Human Blood in vitro - After allowing to the P.V.C. extract at room temperature for 5 Hours and at 10\ulcorner for 24 hours, there was no appreciable difference in Prothrombin Time under these conditions. 8.Clotting Mechanism of Rabbit in vivo - At the termination of 5 days after intraperitoneal injection of the P.V.C. extract, no significant changes in Clotting Time were observed. According to the above results, it could be concluded that the P.V.C. made in Korea was acceptable for parenteral preparation, especially treated with physiologic saline and/or human blood.man blood.

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.297-302
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• 2013

Cryopreservation of epididymal spermatozoa offers a potential tool for rescuing genetic material from males of genetically elite populations. Castration, catastrophic injury, sudden death or any other event that makes semen collection or mating impossible may prematurely terminate a stallion reproduction. Stallion epididymal spermatozoa vary widely in the loss of progressive motility, acrosomal integrity, and viability during freezing and thawing. The objective of this work was to investigate the effect of (1) freezing package types on cryopreservation efficiency, (2) thawing temperatures (37, 56 or $70^{\circ}C$) on Computer Assisted Sperm Analysis (CASA) parameters and (3) post-thawing incubation time (0, 1, 2 or 4h) on castrated stallion epididymis. Post-thawed sperm motility ranged between 59.69% and 64.28% ($56^{\circ}C$ and $37^{\circ}C$) in various thawing temperatures. When stallion epididymis sperm was frozen, straw was better than freezing tube on VCL (Velocity of Curvilinear Line) and VAP (Velocity of Average Path) parameter. Higher percentage of motility was observed at $37^{\circ}C$ thawing temperature even though no significant difference was observed among various temperatures. The motility, VCL, ALH (Amplitude of Lateral Head displacement), VAP, BCF (Beat-Cross Frequency) and STR (Straightness index) parameter of post-thawed sperm were significantly decreased by increasing the incubation time for all thawing temperatures. The present study showed that type of freezing package (Straw vs. Freezing tube) was not significantly different on cryopreservation efficiency. Furthermore, stallion epididymal spermatozoa frozen-thawed at $37^{\circ}C$ for 1 min resulted the highest proportion of motility and velocity movement. In addition, motility and viability of frozen-thawed stallion epididymal spermatozoa were also decreased by incubation.

• 한국해양학회지
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• v.15 no.1
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• pp.49-57
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• 1980

Toxicity of Machete on the mortality of the three different life stages, namely, hatching period, prelarval stage, and juvenile stage of carp(Cyprinus carpio Linne) was observed. The effect of the various exposure times on the mortality of the prelarval stage, and the effect of different temperatures on the toxicity during juvenile stage were also analysed. Hatchability and duration time of the carp were shown to be related to the concentration of Machete. In general, hatchability and duration time were shown to be decreased when the concentration of Machete was increased. For prelarval stage, 24hr and 48hr LC 50, and 24hr and 48hr EC 50 were observed to be 0.86mg/l and 0.43mg/l, and 0.35mg/l and 0.20mg/l, respectively. For juvenile stage, 24hr, 48hr, 96hr, and Incipient LC 50 were found to be 0.625mg/l, 0.365mg/l, 0.180mg/l, and 0.135mg/l, respectively. Apparently it was appeared that eggs were most resistant, and prelarvae were most sensitive to Machete among the three life stages. Although fish were removed from the Machete solution(3.2mg/l, and 1.8mg/l) prior to their death, continuous mortality was observed. Below 1.mg/l range, however, when concentration of Machete was lowered, the toxic effect of Machete on carp was reduced considerably even to the longtime exposure(24hours) Toxicity of Machete appeared to be related to water temperature, and it was observed that higher temperature reflected more toxicity.

• Fire Science and Engineering
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• v.21 no.3
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• pp.15-23
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• 2007

In the events of a fire in the residential building, highly flammable polyurethane foam sofa produce toxic smokes. In this type of fire, the residents of the building can be gotten into the difficulties of evacuating from the fire places or may be to death due to a lot of hot toxic gases. In this study, CFD simulations were carried out to study the effects of the openings of stairwell on the fire characteristics of fire room and stairwell. Also, analysis of fire hazard based on the tenability limits of fire and FED(fractional effective dose) was performed to evaluate the life safety of the residents of the building. In the fire room, maximum temperature was about $290^{\circ}C$, maximum CO concentration was about 4,740 ppm, and the time to incapacitation of residents in fire room was about t=144 s. In the stairwell, temperature and CO concentration in the condition of openings to be open were even lower than those in it to be closed. Time to the tenability limit with respect to smoke visibility in the stairwell with openings, which was open, was shorter than that of it without openings to be open. It has been shown from this study that opening the stairwell openings is able to decrease the fire hazards to the life safety in the multi-story residential building fire.

• Korean journal of applied entomology
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• v.35 no.4
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• pp.297-301
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• 1996

This test has been carried out to evaluate the effect of temperature on the growth of the insecticide susceptible strain, URY-O nomal genotype and insecticide resistant strain, O-RT abnormal genotype, and ABURABI nomal genotype. The nymphal periods were not significantly different between URY-O and O-RY strains at $25^{\circ}C$. At $30^{\circ}C$, susceptible strain URY-O could give birth to offsprings almost nomally, while resistant strain O-RY could not produce any offspring for 20 days which results in nymphal death. The numbers of offsprings of strain URY-O and strain ABURABI were not different between $25^{\circ}C\;and\;28^{\circ}C$, but strain O-RY, when it was reared at $28^{\circ}C$, could produce offsprings only 10% of those at $25^{\circ}C$. Body weight of strain URY-O and strain ABURABI were 0.22mg/female and 0.27mg/female, respectively at $28^{\circ}C$, however that of O-RY was only 0.16mg/female, showing considerable difference between normal and abnormal genotype. Substrain O-RY(+) which has high esterase activity showed poor reproduction ability(0.8 progenies per $G_{1}$ individual than substrain O-RY(-) (3.4 progenies per $G_{1}$ individual) which has low esterase activity at $28^{\circ}C$

• Korean Journal of Food Science and Technology
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• v.22 no.1
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• pp.26-32
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• 1990

Attempts were made to define the characteristics of microbial community as an indicator of Kimchi fermentation. Determination of communities was carried out by simple Gram-stain, followed by direct microcopic counts. In room-temperature $(15^{\circ}C)$ fermentation, microbial succession was occurred in the order of communities of Gram-positive bacteria, yeasts and Gram-negative bacteria. It was characteristic that Gram-positive bacterial community was developed during the production of lactic acid, yeasts community was developed to cause rancidity, and Gram-negative bacterial community was relevant to maceration (or softening) as well as rancidity. The fluctuation of apparent Gram-negative reaction group might be used as a criterion of death or aging of Gram-positive bacterial populations. In low-temperature fermentation $(5^{\circ}C)$, however, it was found that yeasts and Gram-negative bacterial communities did not developed but only Gram-positive bacterial community did. It follows from these results mentioned above that maturity of Kimchi depends on the development of Cram-positive bacterial community. Thus, the size and occurrence of microbial community are avaiable for an indicator of Kimchi fermentation, and also determination of community could be a useful method to predict the maturity.

• Journal of the Korean Institute of Gas
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• v.11 no.4
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• pp.91-97
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• 2007

Recently, during the past five years, accidents of gas boiler using city gas have occurred 7.4 times more than those which use LP gas. The number of accidents has increased since the use of city gas boilers has increased. These boiler accidents resulted in 87% death from poisoning of CO, and casualty of the accidents was 4.3 times more than that of other types of accident. Hence this study makes the cause of accidents clear by separation the exhaust tube which is the cause of CO poisoning. Also, this study will establish the safety of heat-resistant silicon through testing the performance of heat-resistant silicon. The experiment showed that common silicon started hardening at $56^{\circ}C$ while the heat-resistant silicon did not begin carbonization until $606^{\circ}C$. Besides at the temperature of $150^{\circ}C$ which is the normal temperature of exhaust tube, common silicon leaked on the pneumatic test after deterioration, but the heat-resistant silicon maintained its original property. With these results, we judge that we can reduce the casualty by CO poisoning if we use the heat-resistant silicon to the connector of he exhaust tube.

• The Korean Journal of Malacology
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• v.29 no.3
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• pp.207-216
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• 2013

Changes in density of manila clam, Ruditapes philippinarum to environment, catch and recruitment were studied in a few stations (Seonjae, Seongam, Hwangdo and Padori) in the West coast area of Korea from January, 2007 to December, 2009. Water temperature, salinity, dissolved oxygen and pH in the study stations were 0.8 to $31.2^{\circ}C$, 22.1 to 33.7 psu, 5.0 to 12.0 mg/L and 7.39 to 8.99, respectively. The concentrations of dissolved inorganic nitrogen, phosphate and silicate were 0.016 to 1.281 mg/L, 0.004 to 0.093 mg/L and 0.016 to 1.617 mg/L, respectively. Chlorophyll-a ranged from 0.2 to 12.1 ${\mu}g/L$, respectively. Substrata were mainly composed of muddy sand and very poorly sorted in Padori, muddy sand and well sorted in Seonjae and Hwangdo, gravelly muddy sand and poorly sorted in Seongam. Density was high in Seonjae and Seongam, but low in Hwangdo and Padori. In clam culture station, in which spat was naturally produced without sowing seedlings, the living density was decreased by increasing of death and a catch of shellfish, and recruitment was changed. Also, Density affected condition factor and shape of clam. Condition factor was the highest in Hwangdo, in which temperature in the winter and chlorophyll-a were high, and was the lowest in Padori. In the shape of clam, the shape in Seongam was a elongated form, but in Padori was a stunted form.

• Food Engineering Progress
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• v.15 no.3
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• pp.230-234
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• 2011

The objective of this study was to optimize the thermal condition and determine the shelf life of heated Chunbokjang product. The optimum thermal condition of heated Chunbok-jang product was determined by sensory test, and heat penetration curve was obtained by Thermal Microprocessor. Sterilization time was a 21~23 min until $F_0$ value reached 9 min, depending upon the number of abalone. As solid content was reduced and temperature of sterilization was increased, the thermal death time was decreased. The score of sensory test indicated that there was no significant difference in flavor of heated Chunbok-jang product made at different sterilization temperatures (110, 121.1, 125, and $130^{\circ}C$). Heated Chunbok-jang product, however, sterilized at $125^{\circ}C$ showed the highest score in texture and taste values. Salinity and pH were not changed during seven month storages, but texture became firmer, and any microorganism had not been detected from the heated chunbok-jang product during these periods. As a result of storage experiment, the shelf-life of heated Chunbok-jang product was 3-month at room temperature.

• Development and Reproduction
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• v.4 no.2
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• pp.153-160
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• 2000

In this study, genetic variation in the 5 strains of 2N-cont, meiotic-G2N, mitotic-G2N and two types of clones with different genetic backgrounds was investigated by developing their tolerance to water temperature and salinity, which is a physiological trait, into a quantitative trait. The temperature was set at 19$^{\circ}C$, 22.5$^{\circ}C$, 25$^{\circ}C$ and 30$^{\circ}C$, each of which was combined with 0$\textperthousand$, 15$\textperthousand$ and 30$\textperthousand$ of salinity respectively, making 12 groups in all. In the mean survival time (MST), samples with 15$\textperthousand$ of salinity showed the longest survival time at all temperatures. The 2N-cont had the longest 126.16 h followed by clone-11 and clone-15 surviving for 113.22 h and 91.05 h respectively. Gynogenetic diploids showed the shortest 87,32 h and 36.56 h. At 22.5 and 25$^{\circ}C$, MST of each strain was significantly short, showing similar results to those of the groups at 19$^{\circ}C$. The 2N-cont had the longest MST while clones had a longer MST than gynogenetic diploids. This could be due to gynogenesis which causes homozygosis among malignant harmful genes, leading to its appearance in populations and resulting in early death in individuals with such genes. On the other hand, MST of clones was longer than that of gynogenetic fish. This could be because the 1st gynogenetic generation, which is a parental population, has already had its malignant genes removed, while the clones of the 2nd gynogenetic generation have had their superior genes fixed as well as their tolerance and survival improved. When temperature was raised to 22.5$^{\circ}C$ and 25$^{\circ}C$, increase in variation was observed in gynogenetic diploids and decrease in clones in 15$\textperthousand$ of salinity. This shows that such a trait is genetic to a certain extent. Consequently, if this character is developed into a quantitative trait and applied to selective breeding, it could be a useful character to secure superior strains and individuals, and also it would be possible to improve populations genetically through selection.