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Development of Precast Hollow Concrete Columns with Non-Shrink Mortar Grouting Type Splice Sleeve (무수축 모르타르 충진형 슬리브를 사용한 중공 프리캐스트 교각 개발)

  • Cho, Jae-Young;Lee, Young-Ho;Kim, Do-Hak;Park, Jong-Heon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.31 no.3A
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    • pp.215-225
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    • 2011
  • In general, the precast columns can obtain its homogeneous quality as they are produced in a factory with a hollow concrete block type by using high strength concrete, so that they can generate the reduction of dead load. Such a method of precast hollow concrete columns is already implemented in USA and Japan and used for connecting between blocks which use PC tendons. However, it is inevitable to have uneconomical construction with excessive cost in early stage when PC tendons are used. This study aims to develop an economical precast column with high quality and constructability which consists of only splice sleeve and general reinforcing bar without using PC tendons in order to reduce the construction period and cost. To achieve this goal, this study tested the performance of total 5 minimized models in the experiment with the variables such as hollowness, diameter of main reinforcement bar and cross-sectional size for the cross section of precast column by using grouting type splice sleeve which is a new type joint rebar. And it also verified the performance of column in the experiment for a large-sized model in order to overview its applicability by excluding large scale effect.

Effects on Mammalian Tissues and Cells by Sulfur Containing Compounds (황함유 화합물이 동물의 조직과 세포에 미치는 영향)

  • 이기섭;이정채;나상록;정희영;임계택
    • Toxicological Research
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    • v.15 no.1
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    • pp.79-87
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    • 1999
  • To know the stress response and antioxidative effect of sulfur containing compounds, we observed the expression of the stress protein (heat shock protein; inducible protein) from mouse tissues and evaluated the protective effects to hydroxyl radical in mouse brain cell culture. Cysteine, methionine or sodium sulfide was fed by oral administration of 1 ml/per 6hr/three times with 1 mM, 2mM or 3mM to mouse, respectively. After that, the stress proteins were extracted from mouse tissues and analyzed the features of expression. The stress proteins by sulfur containing compounds were showed different aspects in the kinds and concentrations of their compounds, and in the tissues of mouse. In the liver, the stress proteins were appeared at different time on the concentration of sulfur containing compounds and had less than 20 KDa as small molecules. In general, the molecular weights of stress protein in liver, the stress proteins were appeared at different time on the concentration of sulfur containing compounds and had less than 20 KDa as small molecules. In general, the molecular weights of stress protein in the spleen were evaluated from 32KDa to 50KDA, and the induced times were relatively late at high concentration of cysteine, early at low concentration of methionine or sodium sulfide. The stress proteins in mouse muscle were detected mostly between 24hr after treatment of sulfur containing compounds. Their molecular weights were 15~24KDa. In the antioxidative effects of sulfur containing compounds to hydroxyl radical, cell viabilities were measured by 63.2% at 10 $\mu\textrm{M}$, 65.5% at 50 $\mu\textrm{M}$, 68.6% at 100 $\mu\textrm{M}$, 78.3% at 150 $\mu\textrm{M}$, or 83.0% at 200 $\mu\textrm{M}$ of cysteine, respectively. At addition of methionine, the cell viabilities were assessed as 58.1% at 10 $\mu\textrm{M}$, 62.8% at 50 $\mu\textrm{M}$, 75.7% at 100 $\mu\textrm{M}$, 78.6% at 150 $\mu\textrm{M}$, and 79.2% at 200 $\mu\textrm{M}$ after 4hrs exposure with 20mU/ml glucose oxidase (GO) system, while the numbers of live cells to hydroxyl radicals in treatment of sodium sulfide were showed 48.6% at 10 $\mu\textrm{M}$, 54.8% at 100 $\mu\textrm{M}$, 51.8% at 150 $\mu\textrm{M}$, and 51.6% at 200 $\mu\textrm{M}$ in the neuronal cells. In the inhibitory effects on the proliferation of tumor cells, percentages of dead cells of the CT-26 or HeLa cell were generally less than 30% even 48hr after addition of sulfur containing compounds. Conclusively, the results of these experiments indicate that stress protein by sulfur containing compounds can be used as physiological indicator for animal nutrition and for environment, and also that cysteine and methionine can play critical roles as an antioxidant.

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Clinical and Histopathological Findings in Mice Heavily infected with Fibricola seoulensis (Fibricolu seoulensis 중감염 마우스의 임상적 및 조직병리학적 소견)

  • Sun Huh;Jong-Yil Chai;Sung-Tae HONG;Soon-Hyung Lee
    • Parasites, Hosts and Diseases
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    • v.26 no.1
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    • pp.45-54
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    • 1988
  • To observe the clinical course and intestinal histopathology in heavy infection of Fibricola seoulensis, an experimental study was performed in mice. Clinical, experimental infection with 1, 000 metacercariae. On the 11th day after infection, the mice began to die and all of the infected mice were dead by the 16th day. The infected mice showed gradual weight loss. Occult blood was detected after the 10th day. Diarrhea accurred after the 9th day and was recognized in all of the infected mice on the 12th day. Hemoglobin and mean corpuscular hemoglobin decreased significantily after the 12th day, and mean corpuscular hemoglobin concentration decreased in all infected mice. The histopathological changes were more marked in the duodenum than in the jejunum or ileum. Major changes were villous atrophy and crypt hyperplasia, with decreased villus/crypt ratio, inflammatory cell infiltration and stromal edema. The present results suggest that the cause of death of mice heavily infected with F. seoulensis should be malnutrition and severe fluid loss due to malabsorption, together with intestinal bleeding.

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Lichen Diseases on Prunus mume Caused by Colonization of Consortium of Several Crustose Lichens Including Parmotrema autrosinense (지의류 복합체에 의한 매실나무 지의류병)

  • Koh, Young-Jin;Kim, Gyoung-Hee;Jeong, In-Ho;Lim, Myoung-Taek;Park, Hyun-Su;Lee, Gyu-San;Jo, Kyoung-Youn;Hur, Jae-Seoun
    • Research in Plant Disease
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    • v.13 no.3
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    • pp.223-227
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    • 2007
  • Lichen is a symbiotic association which lichen-forming fungi associate with green algae or cyanobacteria capable of producing food by photosynthesis. Lichen is one of the most widely distributed eukaryotic organisms on every kind of surface of soils, rocks and trees. Lichen-forming fungi commonly colonize on the surface of barks of dead or dying trees, which they use merely as a means of support without parasitizing. Lichen consortium of Parmotrema autrosinense and several crustose lichens heavily colonized on the surface of P. mume in Suncheon, Jeonnam province in Korea. The lichen consortium on the surface of P. mume gave the affected trees a dull gray appearance and suppressed their normal growths. Severely affected twigs or branches resulted in the death when the lichen thalli were not rubbed off the barks of P. mume. Twigs or branches of P. mume were recovered from blight syndrome when the lichen thalli were rubbed off, The recovered twigs or branches became healthy and clean when $Tween^{(R)}$ 20 or thiophanate-methyl paste were treated on the barks of P. mume. This is the first report of lichen diseases on P. mume caused by colonization of consortium of several crustose lichens including P. autrosinense.

Effects of Fusarium circinatum on Disease Development and Gas Exchange in the Seedlings of Pinus spp. (푸사리움가지마름병균 Fusarium circinatum이 소나무류 묘목의 병 진전과 침엽의 가스교환에 미치는 영향)

  • Woo, Kwan-Soo;Yoon, Jun-Hyuck;Han, Sang-Urk;Woo, Su-Young
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.177-183
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    • 2011
  • Four-year-old seedlings of Pinus thunbergii, Pinus densiflora and Pinus rigida were inoculated with Fusarium circinatum isolate (FT-7), the pitch canker fungus, from P. thunbergii, to evaluate the effects of the pathogen on disease development and gas exchange rate. Needle dehydration was evident on 2 of 10 seedlings of P. thunbergii and P. rigida at 18 and 21 days after inoculation, respectively, while no symptoms were observed in P. densiflora seedlings throughout the experiment. Gas exchange stopped completely in 4 of 5 measured seedlings of P. thunbergii and 2 of 5 measured seedlings of P. rigida at 25 days after inoculation, and in the remaining 3 seedlings of P. rigida at 39 days after inoculation. Disease development in P. thunbergii seedlings was faster than that in P. rigida seedlings. By the time, the experiment was ended at 78 days after inoculation, 9 of 10 seedlings of P. rigida and 8 of 10 seedlings of P. thunbergii seedlings treated with FT-7 was almost dead, but all seedlings of P. densiflora were still healthy. We suggest that P. densiflora is resistant to F. circinatum in the current study, and gas exchange rate of the species after inoculation does not differ significantly compared to that of untreated control.

Control of the Root-Knot Nematode (Meloidogyne spp.) on Cucumber by a Liquid Bio-Formulation Containing Chitinolytic Bacteria, Chitin and Their Products (키틴분해세균, 키틴 및 그들의 산물이 함유된 미생물제에 의한 오이의 뿌리혹선충(Meloidogyne spp.) 방제)

  • Ha, Woo Jong;Kim, Young Cheol;Jung, Hyuncha;Park, Seur Kee
    • Research in Plant Disease
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    • v.20 no.2
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    • pp.112-118
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    • 2014
  • A liquid bio-formulation containing chitinolytic bacteria, chitin and their products was assessed for its potential biological control against root-knot nematodes on cucumber. The bio-formulation was prepared by cultures of three chitinolytic bacteria, Chromobacterium sp. strain C-61, Lysobacter engymogenes and Serratia plymuthica in minimal medium supplemented with chitin. Under pot conditions, the bio-formulation showed better growth of cucumber plants, and less root galls and population density of Meloidogyne spp. than control media without the bio-formulation. In a greenhouse, 75-fold diluted bio-formulations were treated instead of water around cucumber plants through hoses for drip irrigation six times at 5-day intervals from the transplanting date. After 30 and 60 days, the treatment provided about 7% and 10% enhancement in the plant height and about 78% and 69% reduction in population density of Meloidogyne spp. in the rhizosphere, respectively. In addition, the experiments showed that the control effects occurred only in the soils contacted with the bio-formulation. Undiluted bio-formulations were drenched three times at 10-day intervals around cucumber plants severely infested with Meloidogyne spp. The treatment showed about 37% plant enhancement without dead plants compared with 37% death in the untreated control, and about 82% nematode reduction. These results suggest that the bio-formulation can be practically used to control the root-knot nematode on cucumber.

The Effect of Plant Coverage on the Constructed Wetlands Performance and Development and Management of Macrophyte Communities (식생피도가 인공습지의 질소 및 인 처리효율에 미치는 영향과 습지식물의 조성 및 관리)

  • Ham, Jong-Hwa;Kim, Hyung-Chul;Koo, Won-Seok;Shin, Hyun-Bhum;Yun, Chun-Gyeong
    • Korean Journal of Ecology and Environment
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    • v.38 no.3 s.113
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    • pp.393-402
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    • 2005
  • The field scale experiment was performed to examine the effect of plant coverage on the constructed wetland performance and recommend the optimum development and management of macrophyte communities. Four sets (each set of 0.88 ha) of wetland (0.8 ha) and pond (0.08 ha) systems were used. Water flowing into the Seokmoon estuarine reservoir from the Dangjin stream was pumped into wetland system. Water depth was maintained at 0.3 ${\sim}$ 0.5 m and hydraulic retention time was managed to about 2 ${\sim}$ 5 days; emergent plants were allowed to grow in the wetlands. After three growing seasons of the construction of wetlands, plant coverage was about 90%, even with no plantation, from bare soil surfaces at the initial stage. During the start up period of constructed wetlands, lower water levels should be maintained to avoid flooding newly plants, if wetland plants are to be started from germinating seeds. Effluent T-N concentration in low plant coverage wetland was higher in winter than high plant coverage wetland, whereas no T-P effluent concentration and removal efficiency difference was observed within 15% plant coverage. Dead vegetation affected nitrogen removal during winter because it is a source of organic carbon which is an essential parameter in denitrification. Biomass harvesting is not a realistic management option for most constructed wetland systems because it could only slightly increase the removal rate and provide a minor nitrogen removal pathway due to lack of organic carbon.

Estimation of Accumulated Degree Days Required for the Development of $Cloeon$ $dipterum$ (Ephemeroptera: Baetidae) in an Experimental Tub Under Field Conditions. (야외 실험 수조에서 추정한 연못하루살이($Cloeon$ $dipterum$)(Ephemeroptera: Baetidae)의 발육 유효적산온도)

  • Lee, Cha-Young;Kim, Dong-Gun;Choe, Lak-Jung;Baek, Min-Jeong;Yoon, Tae-Joong;Bae, Yeon-Jae
    • Korean Journal of Ecology and Environment
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    • v.45 no.1
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    • pp.123-128
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    • 2012
  • We aimed to estimate the accumulated degree days required for the development of $Cloeon$ $dipterum$ (Ephemeroptera: Baetidae) in an experimental tub under field conditions from August 7 to October 10 in 2011. After 4 days in a tub setting, a dead female adult was found on the water surface at which oviposition had presumably occurred. Adults emerged from 39 days to 61 days after the oviposition date. The total number of emerged adults was 229, and the sex ratio (number of females : total population) was 0.45, although not statistically significant ($p$ >0.05). The males generally emerged earlier than females. Assuming that the basal temperature ($T_b$) value is $0^{\circ}C$, we estimated the number of accumulated degree days required for the development of $C.$ $dipterum$ to be $1,221.8{\pm}116.0$ DD (mean${\pm}$SD). Since the annual number of accumulated degree days in the reference wetland was measured to be 5,650.0 DD, we estimated that $C.$ $dipterum$ is multivoltine, with a maximum of 4 cohorts in 1 year in natural habitats.

Possibility of Control of Turfgrass Insect Pest, Popillia quadriguttata(Coleoptera : Rutelidae) Using Pheromone Trap in Golf Course (페로몬 트랩을 이용한 골프장 잔디해충 녹색콩풍뎅이의 방제가능성)

  • Kim, Jae-Ho;Lee, Jae-Pil;Ham, Suon-Kyu;Kim, Doo-Hwan;Yeom, Ju-Rip;Lee, Dong-Woon
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.45-60
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    • 2009
  • Occurrence ecology and possibility of mass trapping of Popillia quadriguttata, one of the most serious turfgrass insect pest in domestic golf courses was investigated using pheromone traps in Gimpo and Jinhae. Peak period of adult occurrence of P. quadriguttata was late June and early July in Gimpo Seaside Country Club from 2006 to 2008. It was early and middle July in Youngwon Country Club in 2006. The first catched day of P. quadriguttata adult was 20 June, while the last day was 10 September in Gimpo Seaside Country Club, 2006. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap per day in peak period of occurrence were 18.2, 25.7, 29.0, and 15.7 at 2005, 2006, 2007 and 2008 respectively. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap were more in fairways or roughs than tees or greens. Possibility of control of scarab beetle using pheromone trap with pesticide treatment was investigated against oriental beetle, Blitopertha orientalis in Dongrae Benest Golf Club. Mean number of dead oriental beetle adult were more higher(2.9 folds) in fenitrothion EC treatment than fenitrothion EC treatment after set oriental beetle pheromone trap.

Presentation of Retinoblastoma Patients in Malaysia

  • Subramaniam, Shridevi;Rahmat, Jamalia;Rahman, Norazah Abdul;Ramasamy, Sunder;Bhoo-Pathy, Nirmala;Pin, Goh Pik;Alagaratnam, Joseph
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.18
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    • pp.7863-7867
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    • 2014
  • Background: Retinoblastoma is a rare type of cancer that usually develops in early childhood. If left untreated it can cause blindness and even death. The aim of this study is to determine sociodemographic and clinical features of retinoblastoma patients and also to determine the treatment pattern and outcome in Malaysia. Materials and Methods: Data for this study were retrieved from the Retinoblastoma Registry of the National Eye Database (NED) in Malaysia. Hospital Kuala Lumpur, Hospital Umum Kuching, Sarawak and Hospital Queen Elizabeth, Kota Kinabalu were the major source data providers for this study. Data collected in the registry cover demography, clinical presentation, modes of treatment, outcomes and complications. Results: The study group consisted of 119 patients (162 eyes) diagnosed with retinoblastoma between 2004 and 2012. There were 68 male (57.1%) and 51 (42.9%) female. The median age at presentation was 22 months. A majority of patients were Malays (54.6%), followed by Chinese (18, 5%), Indians (8.4%), and indigenous races (15.9%). Seventy six (63.8%) patients had unilateral involvement whereas 43 patients had bilateral disease (36.1%). It was found that most children presented with leukocoria (110 patients), followed by strabismus (19), and protopsis (12). Among the 76 with unilateral involvement (76 eyes), enucleation was performed for a majority (79%). More than half of these patients had extraocular extension. Of the 40 who received chemotherapy, 95% were given drugs systemically. Furthermore, in 43 patients with bilateral involvement (86 eyes), 35 (41%) eyes were enucleated and 17 (49%) showed extraocular extension. Seventy-two percent of these patients received systemic chemotherapy. The patients were followed up 1 year after diagnosis, whereby 66 were found to be alive and 4 dead. Sixteen patients defaulted treatment and were lost to follow-up, whereas follow-up data were not available in 33 patients. Conclusions: Patients with retinoblastoma in this middle-income Asian setting are presenting at late stages. As a result, a high proportion of patients warrant aggressive management such as enucleation. We also showed that a high number of patients default follow-up. Therefore, reduction in refusal or delay to initial treatment, and follow-up should be emphasized in order to improve the survival rates of retinoblastoma in this part of the world.