• International Journal of Concrete Structures and Materials
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• 제10권2호
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• pp.149-161
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• 2016

This paper presents an experimental work to study the flexural strength of reinforced concrete (RC) beams strengthened by partially de-bonded near surface-mounted (NSM) fiber reinforced polymer (FRP) strip with various de-bonded length. Especially, considering high anchorage capacity at end of a FRP strip, the effect of de-bonded region at a central part was investigated. In order to check the improvement of strength or deformation capacity when the bonded surface area only increased without changing the FRP area, single and triple lines of FRP were planned. In addition, the flexural strength of the RC member strengthened by a partially de-bonded NSM FRP strip was evaluated by using the existing researchers' strength equation to predict the flexural strength after retrofit. From the study, it was found that where de-bonded region exists in the central part of a flexural member, the deformation capacity of the member is expected to be improved, because FRP strain is not to be concentrated on the center but to be extended uniformly in the de-bonded region. Where NSM FRP strips are distributed in triple lines, a relatively high strength can be exerted due to the increase of bond strength in the anchorage.

• Animal cells and systems
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• 제15권1호
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• pp.29-36
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• 2011

Insect lysozymes are basic, cationic proteins synthesized in fat body and hemocytes in response to bacterial infections and depolymerize the bacterial cell wall. The c-type lysozyme of the insect Spodoptera litura (SLLyz) is a single polypeptide chain of 121 residues with four disulfide bridges and 17 rare codons and is approximately 15 kDa. The full-length SLLyz cDNA is 1039 bp long with a poly(A) tail, and contains an open reading frame of 426 bp long (including the termination codon), flanked by a 54 bp long 5' UTR and a 559 bp long 3' UTR. As a host for the production of high-level recombinant proteins, E. coli is used most commonly because of its low cost and short generation time. However, the soluble expression of heterologous proteins in E. coli is not trivial, especially for disulfide-bonded proteins. In order to prevent inclusion body formation, GST was selected as a fusion partner to enhance the solubility of recombinant protein, and fused to the amplified products encoding mature SLLyz. The expression vector pGEX-4T-1/rSLLyz was then transformed into E. coli BL21(DE3)pLysS for soluble expression of rSLLyz, and the soluble fusion protein was purified successfully. Inhibition zone assay demonstrated that rSLLyz showed antibacterial activity against B. megaterium. These results demonstrate that the GST fusion expression system in E. coli described in this study is efficient and inexpensive in producing a disulfide-bonded rSLLyz in soluble, active form, and suggest that the insect lysozyme is an interesting system for future structural and functional studies.

• 한국터널지하공간학회 논문집
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• 제10권2호
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• pp.105-118
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• 2008

숏크리트는 터널에서 사용되는 중요한 지보재이다. 숏크리트와 암반의 접착상태는 터널의 안정성 및 사용성에 큰 영향을 끼치는 중요한 평가 요소이다. NATM공법을 이용한 터널 굴착시 굴착면 빛 벤치부에서 발파에 의해 숏크리트가 부착력을 잃고 암반으로부터 탈락되거나 공동이 형성되는 경우 숏크리트 자체의 파괴뿐만 아니라 터널의 전체적인 안정에도 악영향을 미친다. 숏크리트의 접착상태는 완전 접착, 접착력 상실, 그리고 공동으로 분류할 수 있다. 본 연구에서는 비파괴 시험인 충격반향기법(Impact-Echo)을 이용하여 숏크기트와 암반의 접착상태를 평가하고자 하였다. 범용 유한요소 해석 프로그램인 ABAQUS를 이용하여 충격반향시험에 대한 수치해석을 수행하였다. 수치해석으로부터 획득된 신호를 시간영역, 주파수 영역 및 시간-주파수 영역에서 각각 해석하여 숏크리트와 암반의 접착상태에 따른 신호특성을 분석하였다. 분석결과 능동적 신호 처리 기법인 Short-Time Fourier Transform(STFT)을 이용하여 숏크리트 배면의 접착상태를 효과적으로 예측할 수 있었다. 숏크리트 배면의 접착상태가 불량할수록 시간영역 신호의 최대 진폭 이후 첫 진폭이 커지며, 주파수 영역에서 최대 에너지가 커진다. 또한 뚜렷한 공진 주파수가 나타나므로 숏크리트의 두께의 역산이 가능해진다. 시간-주파수 영역에서 윤곽선은 시간축에 평행한 형상을 나타낸다. 또한 완전 접착조건에서 지반 종류에 따른 신호특성도 분석하였다. 지반조건이 불량할수록 시간-주파수 영역에서 시간축과 평행한 윤곽선의 길이가 길어지며 그 주파수 대역은 10 kHz 이하의 저주파수 영역에서 나타난다.

• Nuclear Engineering and Technology
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• 제50권8호
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• pp.1330-1338
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• 2018

High-density UMo/Zr monolithic nuclear fuel plates have a promising application prospect in high flux research and test reactors. The solid state welding method called co-rolling is used for their fabrication. Hot co-rolling simulations for the composite blanks of UMo/Zr monolithic nuclear fuel plates are performed. The effects of coupon sizes and mechanical property parameters on the contact pressures between the to-be-bonded surfaces are investigated and analyzed. The numerical simulation results indicate that 1) the maximum contact pressures between the fuel coupon and the Zircaloy cover exist near the central line along the plate length direction; as a whole the contact pressures decrease toward the edges in the plate width direction; and lower contact pressures appear at a large zone near the coupon corner, where de-bonding is easy to take place in the in-pile irradiation environments; 2) the maximum contact pressures between the fuel coupon and the Zircaloy parts increase with the initial coupon thickness; after reaching a certain thickness value, the contact pressures hardly change, which was mainly induced by the complex deformation mechanism and special mechanical constitutive relation of fuel coupon; 3) softer fuel coupon will result in lower contact pressures and form interfaces being more out-of-flatness.