• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.510-515
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• 2018

Hwangmichal, a yellow waxy corn hybrid, is a single cross hybrid developed in 2014. This hybrid was created by crossing the seed parent KY30 and pollen parent KY9. Hwangmichal has yellow kernels with a conico-cylindrical ear shape. The days to silking of Hwangmichal were similar to that of Ilmichal, a check hybrid. The ear height ratio of Hwangmichal was 50%, which was lower than that of Ilmichal, and its number of tillers was less than that of Ilmichal. The ear size of Hwangmichal was smaller than that of Ilmichal and the kernel set ratio was 89%. Its sensory evaluation was better than that of Ilmichal. The carotenoid content of Hwangmichal was $32.2{\mu}g/g$, which was much higher than that of Ilmichal. Lodging resistance of Hwangmichal was better than that of Ilmichal. Although the number of fresh ears of Hwangmichal was more than that of Ilmichal, the weight of fresh ears was lower than that of Ilmichal. The flowering period of its parent lines was well matched and seed production was 3:1 at a planting density of parent lines of more than 2:1. It is adaptable to the whole country except for Jeju-do. The plant variety protection right of Hwangmichal was registered in June 2017, and its grant number is 6728.

• BMB Reports
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• v.39 no.3
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• pp.297-303
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• 2006

As lipolytic enzymes, GDSL lipases play an important role in plant growth and development. In order to identify their functions and roles, the full-length cDNA of a GDSL lipase gene, designated BnLIP2, was isolated from Brassica napus L. BnLIP2 was 1,300 bp long, with 1,122 bp open reading frame (ORF) encoding 373 amino acid residues. Sequence analysis indicated that BnLIP2 belonged to GDSL family. Southern blot analysis indicated that BnLIP2 belonged to a small gene family in rapeseed genome. RT-PCR analysis revealed that BnLIP2 was a tissue-specific expressing gene during reproductive growth and strongly expressed during seed germination. BnLIP2 expression could not be detected until three days after germination, and it subsequently became stronger. The transcript of this gene was deficient in root of seedlings growing at different stages. When juvenile seedlings were treated by methyl jasmonate (MeJ), salicylic acid (SA) and naphthalene acetic acid (NAA), BnLIP2 expression could not be induced in root. Our study implicates that BnLIP2 probably plays an important role in rapeseed germination, morphogenesis, flowering, but independent of root growth and development.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.78-78
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• 2022

Food productivity in North Korea is about 50% lower than in South Korea. In order to increase the productivity of major crops, it is necessary to develop early maturing, disease resistance, and high-yielding varieties and apply them early. Since the late 1990s, North Korea has been actively developing potatoes, rice and com as major food crops, and soybeans are considered important as a protein-supplying crop. Domestic cultivated varieties, which are expected to be most adaptable eco-climatologically, are mainly selected from soil with high nutrient soil. It is necessary to test separately for adaptability in low organic soil. So it is very necessary to apply technology to improve soil improvement through rotational crop selection in the middle and long-term. Therefore, this study was conducted to test the adaptability to low organic soils of domestic cultivars and to select varieties. In 2021 there are twenty two (22) varieties of soybeans were grown in low organic soil at the field of Chungbuk National University. This year twenty two (22) varieties of soybeans were also grown in low organic soil at the field of Chungbuk National University. Sowing was done on June 10, the planting distance was 70cm × 15cm, after opening the cotyledons fully, the soybeans were thinned and leaving two plants per hole. In addition, various types of growth characteristics and quantitative components were investigated to evaluate the adaptability to low organic soil of domestic varieties. This study was conducted to investigate the growth characteristics and quantitative components of soybean varieties grown in low organic soil. The flowering period of 22 varieties of soybeans was about 14 days from July 22 to August 4. The flowers of the beans were white, purple, light purple and the pubescence color was gray and brown where most of them were gray. The highest plant height was up to 130.4 cm and lowest was 20.3 cm, highest stem length was up to 119.5 cm and lowest was 15.3 cm. Highest first pod height (FPH) was up to 34.0 cm and lowest was 3.0 cm. Highest stem diameter was up to 15.76mm and lowest was 1.76 mm. Number of main stem nodes was up to 19 and at least 1. Number of branch was up to 10 and at least 0. The number of pod per plant was up to 121. Bacterial pustule has been spread in soybean field.

• Research in Plant Disease
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• v.20 no.2
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• pp.79-86
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• 2014

In order to seek more eco-friend, economic and safer quarantine method than current methyl bromide fumigation, the convergent treatment with 200 Gy of gamma irradiation and several chemicals such as nano-siver particles (NSS), sodium dichloroisocyanurate (NaDCC) was tried on the cuttings of lily in the packing of catonnage box for export. With 6 independent experiments of gamma irradiation on the three lily cultivars, cvs. Siberia, Le reve and Sorbonne, incidence and severity of lily leaf blight was investigated on leaves and petals at 8-d after infection. 200 Gy of gamma irradiation decreased at 13-25% of severity on the leaf of Sorbonne, but it increased at 2-5% of severity on the leaf of Siberia and Le reve. Chemical substitutes such as NSS and NaDCC were not effective to control of lily blight on cuttings. By 200 Gy of gamma irradiation treatment, chlorophyll contents were statistically significantly decreased at 12-d after irradiation and the longevities vaselife of fully open flower of Siberia and Sorbonne were increased at 0.4 to 1.2 days. In addition, the relative fresh weights of the gamma irradiated cuttings were severely dried compared to the non-irradiated control. On the other hands, the symptoms of phyto-toxicity of high dose gamma irradiation at 1 or 2 kGy on cv. Siberia were to be blight at the tip of bloom, bent necks of flower, and delayed the process of flowering.

• Journal of Applied Biological Chemistry
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• v.55 no.4
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• pp.227-234
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• 2012

We observed that oil began to accumulate at 25 seed days after flowering (DAF) and reached the maximum potential at 35 seed DAF of oilseed rape, and the greatest weight of 100 seeds was obtained at 35 seed DAF. To survey a broad analysis of gene expression in developing embryos of Brassica napus, the Bn 300k microarray have been constructed. The Bn 300k Microarrary was designed from 80,696 unigenes clustered from 543,448 ESTs and 780 cDNA at NCBI. These arrays have been hybridized in a series of experiments with probes derived from seeds and leaf of B. napus. Approximately 8.5% of the 7,000 genes were expressed as ratios 2-fold higher in seed (25 DAF) than leaves and 0.4% at ratios 10. Also we observed that storage and cell differentiation-related genes were highly expressed at 10 DAF, whereas energy-related genes including fatty acid metabolism were increased up depending on seed maturation using Microarray, which was confirmed by reverse transcriptase polymerase chain reaction. These results suggest that B. napus arrays provide a very useful data set of seed-specific expression that can be further analyzed by examination of the promoter regions of these genes and help our understanding of the complex regulatory network in developing seeds.

• Journal of Life Science
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• v.30 no.11
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• pp.973-982
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• 2020

In this study, we examined inhibition of adipocyte differentiation associated with the administration of camphor, a substance identified in extracts of the flowering plant Chrysanthemum indicum L. (CI). No camphor-mediated cytotoxicity was observed over a period of 1-10 days in studies targeting cells of the 3T3-L1 adipocyte-like line. Experiments that featured siRNA-mediated suppression of the transmembrane proteins Patched (PTCH) and Smoothened (SMO) resulted in inhibition and activation of differentiation, respectively. Interestingly, inhibition of PTCH typically activates SMO protein targeting and serves to activate hedgehog (HH)-mediated signaling. The results of our study suggest that activation of HH-mediated signaling can inhibit adipocyte differentiation. Furthermore, expression of glioma-associated oncogene homologue 1 (Gli1) was detected by flow cytometry in 62.7±1.5% of cells in response to administration of Lactobacillus rhamnosus (KCTC 3237) and in 60.4±2.2% of cells in response to camphor; these levels are higher than those detected in undifferentiated controls (24.9±3.1%). No change in the state of fermented camphor was identified by gas chromatography-mass spectrometry (GC-MS), but a 15.41% quantitative increase was confirmed in KCTC 3237. Overall, we conclude that administration of camphor resulted in overexpression of SMO and modulated the differential expression of Gli1. Animal studies focused on the impact of camphor as an agent to counteract obesity might be considered in the future. Indeed, camphor and similar physiologically active compounds from fermented CI might be developed as new and effective treatments for obesity.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.6-11
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• 1998

Bean sprout is one of the traditional vegetable in Korea and produced by sprouting soybean (Glycine max L.) cultivars. This experiments was conducted to investigate the ecological characteristics in collected small grain soybean lines from Chungnam province region. Thirty varieties were used in this study. Seed coat color of collected small soybean lines showed eight different types. Major seed coat color type was yellow as 8 lines of total 35 lines. The stem height, number of nodes and number of branches averaged 58.5 cm, 16.5 nodes and 11.8 branches, respectively. The average number of main stem pods and number of branching pod were appeared 43.1 and 62.7 respectively. One hundred seed weight averaged 9.9g which was lower than 10~12g of sprouting soybean cultivars as breeding goal of Korea. The average of node width showed 13.2cm and long stem open types and short stem close types were classified 1 line and 14 lines. The flower dates of collecting small soybean lines were between 8 Aug. and 13 Aug. and flowering duration was 74~85 days. The date of maturity were between 12 Sep. and 12 Oct., and maturity duration showed great variation among cultivated soybean lines.

• The Korean Journal of Mycology
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• v.45 no.1
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• pp.74-82
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• 2017

Lisianthus (Eustoma grandiflorum) is a flowering ornamental plant used widely in Korea. In 2015, wilting, damping-off, stunting, and root rot symptoms were observed in lisianthus plants of Yeoju and Gimhae, Korea. Affected seedlings appeared yellow and showed poor development of root systems in the field and in nursery boxes. Furthermore, affected plants were yellow, stunted, and died at approximately 2-3 months after transplanting. Fusarium species were consistently isolated from the basal stems of diseased plants. Nine isolates were identified as Fusarium solani based on morphological characteristics. Macroconidia of isolates were relatively wide, straight-to-slightly curved, and microconidia formed in false heads on long monophialides. Abundant chlamydospores were produced at the middle or tips of hyphae. To confirm this identification, a molecular analysis of the translation elongation factor 1 alpha (TEF) and RNA polymerase II subunit (RPB2) genes was conducted. The sequences of TEF and RPB2 showed 99.2-99.9% and 98.0-98.1% similarity, respectively, to those of reference F. solani strains in NCBI GenBank. Pathogenicity was tested using root dipping inoculation of healthy lisianthus seedlings. Symptoms were observed within 7 days of inoculation only in inoculated plants. This is the first report of F. solani causing Fusarium root rot on lisianthus in Korea.

• Journal of agriculture & life science
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• v.44 no.5
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• pp.9-13
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• 2010

This study was conducted to identify the possibility of utilization of coconut substrates for nutrition culture of cut-chrysanthemum. The materials of substrate were composed of dust, fiber, and chip from coco-nut fruit. Dust was used in dust (100%), and dust+chip and +fiber were used in the ratio of 7:3 (v:v), respectively, as coconut mixture substrate. Perlite was used as control in this experiment. Water content in the perlite medium was lower than in dust substrate. The pH of all coconut substrates ranged from 6.5 to 5.8, whereas perlite substrate ranged from 7.3 to 6.7. While, EC of dust substrates shown to be highest but perlite substrate was lowest. The growth of chrysanthemum such as stem length, leaf area, and dry matter showed better results in coconut substrates than that of perlite and dust. However, there was no differences days to in flowering among treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.403-410
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• 2021

The present study investigated the quality characteristics of Perilla frutescens Britt. seed compounds according to the cultivar and sowing dates. In the present experiment, five Perilla cultivars, namely 'Dayu', 'Deulsaem', 'Deulhyang', 'Baekjin', 'Sodam', were planted on three different dates, including May 30, June 20, and July 10. The contents of key compounds and phenols varied depending on the cultivar and sowing date. With the delayed sowing time, the days to flowering and maturity of the culitvar decreased, while the seed weight and hardness increased. Crude protein content increased but crude lipid content decreased with the delay in the sowing time. However, there was no interaction of different sowing dates with the fatty acid content, which remained stable compared with the other values. Overall, the total polyphenol content was higher in Perilla seeds sown on May 30.