The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Characterization of Fusarium solani Causing Fusarium Root Rot of Lisianthus in Korea

꽃도라지 뿌리썩음병을 일으키는 Fusarium solani 의 특성

  • Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Hong, Sung Kee (Microbial Safety Team, National Institute of Agricultural Sciences) ;
  • Lee, Young Kee (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Jeomsoon (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Lee, Jae Guem (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kim, Hyo Won (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Kang, Eun Hye (Crop Protection Division, National Institute of Agricultural Sciences) ;
  • Lee, Eun Hyeong (Crop Protection Division, National Institute of Agricultural Sciences)
  • 최효원 (국립농업과학원 작물보호과) ;
  • 홍성기 (국립농업과학원 유해생물팀) ;
  • 이영기 (국립농업과학원 작물보호과) ;
  • 김점순 (국립농업과학원 작물보호과) ;
  • 이재금 (국립농업과학원 작물보호과) ;
  • 김효원 (국립농업과학원 작물보호과) ;
  • 강은혜 (국립농업과학원 작물보호과) ;
  • 이은형 (국립농업과학원 작물보호과)
  • Received : 2016.10.14
  • Accepted : 2017.02.02
  • Published : 2017.03.01
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Abstract

Lisianthus (Eustoma grandiflorum) is a flowering ornamental plant used widely in Korea. In 2015, wilting, damping-off, stunting, and root rot symptoms were observed in lisianthus plants of Yeoju and Gimhae, Korea. Affected seedlings appeared yellow and showed poor development of root systems in the field and in nursery boxes. Furthermore, affected plants were yellow, stunted, and died at approximately 2-3 months after transplanting. Fusarium species were consistently isolated from the basal stems of diseased plants. Nine isolates were identified as Fusarium solani based on morphological characteristics. Macroconidia of isolates were relatively wide, straight-to-slightly curved, and microconidia formed in false heads on long monophialides. Abundant chlamydospores were produced at the middle or tips of hyphae. To confirm this identification, a molecular analysis of the translation elongation factor 1 alpha (TEF) and RNA polymerase II subunit (RPB2) genes was conducted. The sequences of TEF and RPB2 showed 99.2-99.9% and 98.0-98.1% similarity, respectively, to those of reference F. solani strains in NCBI GenBank. Pathogenicity was tested using root dipping inoculation of healthy lisianthus seedlings. Symptoms were observed within 7 days of inoculation only in inoculated plants. This is the first report of F. solani causing Fusarium root rot on lisianthus in Korea.

꽃도라지(Eustoma grandiflorum)는 국내에서 장식용 화훼류로 널리 이용되는 절화류이다. 2015년 경기도 여주와 경남 김해 지역에서 꽃도라지가 시들고, 지제부가 잘록해지면서 위축되고, 뿌리가 썩는 증상이 나타났다. 병든 식물체는 포장이나 육묘상자에서 황화되고, 뿌리 발육이 저해되었고, 진전되면 전체적으로 황화되면서 시들고, 위축되며, 결국 2~3개월 안에 완전히 말라 죽는다. 병든 식물체 지제부에서 Fusarium균이 분리되었고, 9개 균주를 단포자 분리하여 형태적 특성을 조사한 결과, Fusarium solani로 동정되었다. 대형포자는 통통하고, 직선형이거나 약간 굽은 초승달 모양이고, 소형분생포자는 긴mono형태의 분생포자원세포에서 false head상으로 형성되었다. 후벽포자는 균사 중간 혹은 끝부분에 풍부하게 형성되었다. 이와 같은 동정 결과는 translation elongation factor 1 alpha (TEF)와 RNA polymerase II subunit (RPB2) 유전자의 염기서열 분석으로 재확인되었다. 그 결과, 분리 균주는 NCBI GenBank에 등록된 F. solani와 TEF 유전자는 99.2~99.9%, RPB2 유전자는 98.0~98.1%의 상동성을 나타내었다. 건전한 꽃도라지 유묘의 뿌리를 포자현탁액에 침지 접종하여 병원성 검정을 수행한 결과, 접종 7일 이내에 접종한 식물체에서만 병징이 관찰되었다. 따라서 이 병을 F. solani에 의한 꽃도라지 뿌리썩음병으로 명명하며, 병의 발생을 국내에서 처음으로 보고한다.

Keywords

References

  1. Hahm YI. Occurrence of Fusarium wilt on lisianthus (Eustoma grandiflorum) caused by Fusarium oxysporum f. sp. eustomae. Kor J Plant Pathol 1998;14:188-90.
  2. Lee JW, Kim TJ, Kim JH, Lee HD, Kim SD, Yun T, Paek KY. Effects of plant growth regulators on the growth and cut flower quality of Eustoma grandiflorum cv. plantina violet. J Kor Soc Hortic Sci 2000;41:419-22.
  3. Ministry of Agriculture Food and Rural Affairs. Annual report of floriculture cultivation statistics 2012. Sejong: Ministry of Agriculture Food and Rural Affairs; 2013.
  4. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  5. Tomioka K, Hirooka Y, Takezaki A, Aoki T, Sato T. Fusarium root rot of prairie gentian caused by a species belonging to the Fusarium solani species complex. J Gen Plant Pathol 2011;77:132-5. https://doi.org/10.1007/s10327-011-0295-0
  6. Wolcan S, Lori G, Ronco L. First report of Fusarium solani causing stunt on lisianthus. Plant Dis 2001;85:443.
  7. Truter M, Wehner FC. Crown and root infection of lisianthus caused by Fusarium solani in South Africa. Plant Dis 2004;88:573.
  8. McGovern RJ, Harbaugh BK, Polston JE. Severe outbreaks of Fusarium crown and stem rot of lisianthus in Florida. Phytopathology 1997;87:S64.
  9. Pecchia S. Lisianthus crown and stem rot caused by Fusarium avenaceum in Central Italy. Plant Dis 1999;83:304.
  10. Gerlach W, Nirenberg H. The genus Fusarium: a pictorial atlas. Berlin: Kommissionsverlag P. Parey; 1982.
  11. O'Donnell K, Humber RA, Geiser DM, Kang S, Park B, Robert VA, Crous PW, Johnston PR, Aoki T, Rooney AP, et al. Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycologia 2012;104:427-45. https://doi.org/10.3852/11-179
  12. O'Donnell K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia 2000;92:919-38. https://doi.org/10.2307/3761588
  13. Geiser DM, Del Mar Jimenez-Gasco M, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O'Donnell K. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. Eur J Plant Pathol 2004;110:473-9. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0
  14. Choi HW, Kim JM, Hong SK, Kim WG, Chun SC, Yu SH. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology 2009;37:247-50. https://doi.org/10.4489/MYCO.2009.37.4.247
  15. O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998;95:2044-9. https://doi.org/10.1073/pnas.95.5.2044
  16. O'Donnell K, Sutton DA, Rinaldi MG, Sarver BA, Balajee SA, Schroers HJ, Summerbell RC, Robert VA, Crous PW, Zhang N, et al. Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 2010;48:3708-18. https://doi.org/10.1128/JCM.00989-10