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http://dx.doi.org/10.4489/KJM.20170009

Characterization of Fusarium solani Causing Fusarium Root Rot of Lisianthus in Korea  

Choi, Hyo-Won (Crop Protection Division, National Institute of Agricultural Sciences)
Hong, Sung Kee (Microbial Safety Team, National Institute of Agricultural Sciences)
Lee, Young Kee (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Jeomsoon (Crop Protection Division, National Institute of Agricultural Sciences)
Lee, Jae Guem (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Hyo Won (Crop Protection Division, National Institute of Agricultural Sciences)
Kang, Eun Hye (Crop Protection Division, National Institute of Agricultural Sciences)
Lee, Eun Hyeong (Crop Protection Division, National Institute of Agricultural Sciences)
Publication Information
The Korean Journal of Mycology / v.45, no.1, 2017 , pp. 74-82 More about this Journal
Abstract
Lisianthus (Eustoma grandiflorum) is a flowering ornamental plant used widely in Korea. In 2015, wilting, damping-off, stunting, and root rot symptoms were observed in lisianthus plants of Yeoju and Gimhae, Korea. Affected seedlings appeared yellow and showed poor development of root systems in the field and in nursery boxes. Furthermore, affected plants were yellow, stunted, and died at approximately 2-3 months after transplanting. Fusarium species were consistently isolated from the basal stems of diseased plants. Nine isolates were identified as Fusarium solani based on morphological characteristics. Macroconidia of isolates were relatively wide, straight-to-slightly curved, and microconidia formed in false heads on long monophialides. Abundant chlamydospores were produced at the middle or tips of hyphae. To confirm this identification, a molecular analysis of the translation elongation factor 1 alpha (TEF) and RNA polymerase II subunit (RPB2) genes was conducted. The sequences of TEF and RPB2 showed 99.2-99.9% and 98.0-98.1% similarity, respectively, to those of reference F. solani strains in NCBI GenBank. Pathogenicity was tested using root dipping inoculation of healthy lisianthus seedlings. Symptoms were observed within 7 days of inoculation only in inoculated plants. This is the first report of F. solani causing Fusarium root rot on lisianthus in Korea.
Keywords
Fusarium root rot; Fusarium solani; Lisianthus;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
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1 Hahm YI. Occurrence of Fusarium wilt on lisianthus (Eustoma grandiflorum) caused by Fusarium oxysporum f. sp. eustomae. Kor J Plant Pathol 1998;14:188-90.
2 Lee JW, Kim TJ, Kim JH, Lee HD, Kim SD, Yun T, Paek KY. Effects of plant growth regulators on the growth and cut flower quality of Eustoma grandiflorum cv. plantina violet. J Kor Soc Hortic Sci 2000;41:419-22.
3 Ministry of Agriculture Food and Rural Affairs. Annual report of floriculture cultivation statistics 2012. Sejong: Ministry of Agriculture Food and Rural Affairs; 2013.
4 The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
5 Tomioka K, Hirooka Y, Takezaki A, Aoki T, Sato T. Fusarium root rot of prairie gentian caused by a species belonging to the Fusarium solani species complex. J Gen Plant Pathol 2011;77:132-5.   DOI
6 Wolcan S, Lori G, Ronco L. First report of Fusarium solani causing stunt on lisianthus. Plant Dis 2001;85:443.
7 Truter M, Wehner FC. Crown and root infection of lisianthus caused by Fusarium solani in South Africa. Plant Dis 2004;88:573.
8 Gerlach W, Nirenberg H. The genus Fusarium: a pictorial atlas. Berlin: Kommissionsverlag P. Parey; 1982.
9 McGovern RJ, Harbaugh BK, Polston JE. Severe outbreaks of Fusarium crown and stem rot of lisianthus in Florida. Phytopathology 1997;87:S64.
10 Pecchia S. Lisianthus crown and stem rot caused by Fusarium avenaceum in Central Italy. Plant Dis 1999;83:304.
11 O'Donnell K, Humber RA, Geiser DM, Kang S, Park B, Robert VA, Crous PW, Johnston PR, Aoki T, Rooney AP, et al. Phylogenetic diversity of insecticolous fusaria inferred from multilocus DNA sequence data and their molecular identification via FUSARIUM-ID and Fusarium MLST. Mycologia 2012;104:427-45.   DOI
12 O'Donnell K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia 2000;92:919-38.   DOI
13 O'Donnell K, Sutton DA, Rinaldi MG, Sarver BA, Balajee SA, Schroers HJ, Summerbell RC, Robert VA, Crous PW, Zhang N, et al. Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 2010;48:3708-18.   DOI
14 Geiser DM, Del Mar Jimenez-Gasco M, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O'Donnell K. FUSARIUM-ID v. 1.0: A DNA sequence database for identifying Fusarium. Eur J Plant Pathol 2004;110:473-9.   DOI
15 Choi HW, Kim JM, Hong SK, Kim WG, Chun SC, Yu SH. Mating types and optimum culture conditions for sexual state formation of Fusarium fujikuroi isolates. Mycobiology 2009;37:247-50.   DOI
16 O'Donnell K, Kistler HC, Cigelnik E, Ploetz RC. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc Natl Acad Sci USA 1998;95:2044-9.   DOI