• Asian Pacific Journal of Cancer Prevention
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• v.16 no.8
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• pp.3195-3201
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• 2015

Objectives: To investigate the effects of betaine on HeLa cell growth and apoptosis and molecular mechanisms. Materials and Methods: Concentrations of 0.1, 1.0, 5.0, 20.0, 100.0 mg/ml of betaine were used to evaluate the anticancer efficacy for HeLa cells respectively, and MCF-10A was also detected as a normal diploid cell control. Results: We found that proliferation of HeLa cells was inhibited significantly upon exposure to increasing betaine levels with the MTT test (p<0.05). The percentage of S phase cells in the low dose groups (<5mg/ml) were distinctly higher than in high dose groups, and the rates of Sub-G1 phase were the opposite (p<0.01); A high concentration of betaine (>5.0mg/ml) significantly promoted the apoptosis of HeLa cells (p<0.01). SOD activities of the low dose groups were slightly higher than the control group (p<0.05) and there were obvious synchronicity and correlation among the expression of promoting apoptosis genes Bax, P53, Caspase 3 and apoptosis suppression gene Bcl-2. In response to an apoptosis-inducing stimulus, p53 and cyclin D1 could be activated with blockage of the cell cycle at G1/S or S/G2 checkpoints. Conclusions: Our data showed that betaine could promote HeLa cells proliferation in vitro at low concentrations. In contrast, high concentrations could significantly inhibit cell growth and migration, and induce apoptosis of HeLa cells through caspase 3 signaling and further promoted necrosis. This might imply that betaine exhibits tumoricidal effects and acts as a biological response modifier in cancer treatment by inducing apoptosis and cell cycle arrest in a dose and time-dependent manner.

• Journal of Gifted/Talented Education
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• v.22 no.3
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• pp.679-702
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• 2012

Values can be regarded as an important factor which acts as an indicator showing each person's individual path of life. many researchers have suggested that values function as standards that guide thought and action. Values lead us to take particular positions on social issues and they predispose us to favor one perspective over another. Adolescence is an important period for the establishment of one's values. The values established during such a period become an important factor determining one's right personality and contents of life. In particular, it is important to take a study about the values possessed by gifted and talented children in Korea. There have been several researches regarding the cognitive and affective characteristics of gifted and talented learners in Korea. However, there has not been any research regarding their values in life. This study investigates the characteristics of values shown by 468 high-school students from two special schools for gifted students by using Schwartz's Value Survey Scale which is consisted of 57 value categories and 10 value types. According to the results of this study, the students from the special high-school for gifted children tend to promote such value types as 'autonomy' and 'stimulation' the most. Conversely, the same students tend to deny or ignore such value types as 'power' and 'tradition'. This study provides basic data regarding the values of gifted and talented students as the first research of its kind targeting gifted students in Korea. It has an important meaning for the research related to the characteristics shown by gifted students in Korea.

• International Journal of Oral Biology
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• v.34 no.1
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• pp.7-14
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• 2009

Nitric oxide (NO) acts as an intracellular messenger at the physiological level but can be cytotoxic at high concentrations. The cells within periodontal tissues, such as gingival and periodontal fibroblasts, contain nitric oxide syntheses and produce high concentrations of NO when exposed to bacterial lipopolysaccharides and cytokines. However, the cellular mechanisms underlying NO-induced cytotoxicity in periodontal tissues are unclear at present. In our current study, we examined the NO-induced cytotoxic mechanisms in human gingival fibroblasts (HGF). Cell viability and the levels of reactive oxygen species (ROS) were determined using a MTT assay and a fluorescent spectrometer, respectively. The morphological changes in the cells were examined by Diff-Quick staining. Expression of the Bcl-2 family and Fas was determined by RT-PCR or western blotting. The activity of caspase-3, -8 and -9 was assessed using a spectrophotometer. Sodium nitroprusside (SNP), a NO donor, decreased the cell viability of the HGF cells in a dose- and time-dependent manner. SNP enhanced the production of ROS, which was ameliorated by NAC, a free radical scavenger. ODQ, a soluble guanylate cyclase inhibitor, did not block the SNP-induced decrease in cell viability. SNP also caused apoptotic morphological changes, including cell shrinkage, chromatin condensation, and DNA fragmentation. The expression of Bax, a member of the proapoptotic Bcl-2 family, was upregulated in the SNP-treated HGF cells, whereas the expression of Bcl-2, a member of the anti-apoptotic Bcl-2 family, was downregulated. SNP augmented the release of cytochrome c from the mitochondria into the cytosol and enhanced the activity of caspase-8, -9, and -3. SNP also upregulated Fas, a component of the death receptor assembly. These results suggest that NO induces apoptosis in human gingival fibroblast via ROS and the Bcl-2 family through both mitochondrial- and death receptor-mediated pathways. Our data also indicate that the cyclic GMP pathway is not involved in NO-induced apoptosis.

• Korea journal of population studies
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• v.32 no.1
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• pp.93-111
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• 2009

This study aims to find out changes of marital status and average marital life expectancy in Korea according to educational attainment. The study produces Korean marriage life table to accomplish the goal of study by introducing Wolfbein-Wool style working life table. Specific data utilized in this study are collected from the Population & Housing Census 2005 and Death Census 2005. Educational attainment is divided into four categories to accommodate to this study: elementary school course and lower, middle school course, high school course, college course and higher. Marriage rate, divorce rate, widowhood rate and death rate according to educational attainment are used as basicdata to analyze marital life expectancy. The results of this study are as follows: 1. As subjects age is younger, the average marital life expectancy of the highly educated tends to be higher. The disparity of average marital life expectancy according to educational attainment is apt to become narrow as subjects age is older. However, the gap between people who graduated from middle school and high school in older age group does not distinctive. 2. Males marital life expectancy is higher than females controlling for their educational attainment. 3. Males live with their wife for most of their lives but females live alone more than 10 years in every single educational categories. Based on the above, this study concludes that the average marital life expectancy is differentiated among age and sex according to educational attainment. Marital rate tends to be higher as educational level is higher. Divorce rate is lower in the highlyeducated group as their age is younger but this is apt to reverse as age is older. Furthermore, bereavement rate shows division according to educational attainmentsince one tends to marry other who has similar level of education with him or her. Therefore, educational attainment acts as an significant factor in Korean average life expectancy.

• The Journal of the Acoustical Society of Korea
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• v.40 no.5
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• pp.479-487
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• 2021

In this paper, we propose an Sound Event Detection (SED) model using self-training based on a noisy student model. The proposed SED model consists of two stages. In the first stage, a mean-teacher model based on an Residual Convolutional Recurrent Neural Network (RCRNN) is constructed to provide target labels regarding weakly labeled or unlabeled data. In the second stage, a self-training-based noisy student model is constructed by applying different noise types. That is, feature noises, such as time-frequency shift, mixup, SpecAugment, and dropout-based model noise are used here. In addition, a semi-supervised loss function is applied to train the noisy student model, which acts as label noise injection. The performance of the proposed SED model is evaluated on the validation set of the Detection and Classification of Acoustic Scenes and Events (DCASE) 2020 Challenge Task 4. The experiments show that the single model and ensemble model of the proposed SED based on the noisy student model improve F1-score by 4.6 % and 3.4 % compared to the top-ranked model in DCASE 2020 challenge Task 4, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.6
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• pp.228-238
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• 2019

The purpose of this study is to investigate the relationship between transaction justice and customer performance. In this study, data were gathered from questionnaires from customers and suppliers of manufacturing outsourcing service, and reliability, validity, and confirmatory factor analysis (CFA) were conducted and the hypothesis was verified through the structural equation modeling (SEM) analysis. The results of this study are as follows: 1)Transaction justice has a significant positive effect on customer's business performance. Distribution and Interactional justice have a small effect on business performance, but Procedural justice has a significant positive effect. 2)Transaction justice has a positive effect on partnerships. 3)The partnership between the customer and the supplier has a positive effect on the customer's business performance. 4)Partnership has positive effect on customer's management performance through mediating justice. This study is based on the fact that justice acts as a independent variable in the transactional relationship between the customer and the supplier in the manufacturing outsourcing and this is the first case that empirically investigated the effect of the justice on the customer's management performance. In order to effectively cope with a rapidly changing business environment and effectively improve management performance, it is necessary to make efforts to improve justice, especially procedural justice, by utilizing outsourcing effectively in a global competition. In this respect, this study provides important implications for the both academically and practically.

• Journal of Life Science
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• v.28 no.11
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• pp.1268-1276
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• 2018

The cytidine analog decitabine (DEC) acts as a nucleic acid synthesis inhibitor, whereas ammonium pyrrolidine dithiocarbamate (PDTC) is an inhibitor of nuclear factor-${\kappa}B$. The aim of this study was to investigate the possible synergistic inhibitory effect of these two inhibitors on proliferation of human gastric cancer AGS cells. The inhibitory effect of PDTC on AGS cell proliferation was significantly increased by DEC in a concentration-dependent manner, and this inhibition was associated with cell cycle arrest at the G2/M phase and the induction of apoptosis. This induction of apoptosis by the co-treatment with PDTC and DEC was related to the induction of DNA damage, as assessed by H2AX phosphorylation. Further studies demonstrated that co-treatment with PDTC and DEC induced the disruption of mitochondrial membrane potential, increased the generation of intracellular reactive oxygen species (ROS) and the expression of pro-apoptotic Bax, and down-regulated the expression of anti-apoptotic Bcl-2, ultimately resulting in the release of cytochrome c from the mitochondria into the cytoplasm. Co-treatment with PDTC and DEC also activated caspase-8 and caspase-9, which are representative caspases of the extrinsic and intrinsic apoptosis pathways. Co-treatment also activated caspase-3, which was accompanied by proteolytic degradation of poly (ADP-ribose) polymerase. Taken together, these data clearly indicated that co-treatment with PDTC and DEC suppressed the proliferation of AGS cells by increasing DNA damage and activating the ROS-mediated extrinsic and intrinsic apoptosis pathways.

• Korean Journal of Remote Sensing
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• v.34 no.6_2
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• pp.1229-1238
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• 2018

We examined if a state of sea-ice in Bering Sea acts as a prelude of variation in that of Chukchi Sea by using satellites-based Arctic sea-ice concentration time series. Datasets consist of monthly values of sea-ice concentration during 36 years (1982-2017). Time series analysis armed with Transfer entropy is performed to describe how sea-ice data in Chukchi Sea is affected by that in Bering Sea, and to explain the relationship. The transfer entropy is a measure which identifies a nonlinear coupling between two random variables or signals and estimates causality using modification of time delay. We verified this measure checked a nonlinear coupling for simulated signals. With sea-ice concentration datasets, we found that sea-ice in Bering Sea is influenced by that in Chukchi Sea 3, 5, 6 months ago through the transfer entropy measure suitable for nonlinear system. Particularly, when a sea-ice concentration of Bering Sea has a local minimum, sea ice concentration around Chukchi Sea tends to decline 5 months later with about 70% chance. This finding is considered to be a process that inflow of Pacific water through Bering strait reduces sea-ice in Chukchi Sea after lowering the concentration of sea-ice in Bering Sea. This approach based on information theory will continue to investigate a timing and time scale of interesting patterns, and thus, a coupling inherent in sea-ice concentration of two remote areas will be verified by studying ocean-atmosphere patterns or events in the period.

• Korean Security Journal
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• no.13
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• pp.471-485
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• 2007

This study is performed to find out what type instructions are produced to players by coaches and what effects are resulted in cooperative spirit of the concerned group. Furthermore the study has its aims at advancing instructors' skills by using finding of it. The study used 'SPSS 11.0 FOR WINDOW - Statistical Package' to analyze the collected samples and dealt with data of 174 individuals. Statistical analysis of the research for hypothesis verification was about frequency, trust level, mutual relationship, variables, and T-verification. The meaningful level for any result was ranged within 95%(p< .05), 99%(p<.01). The finding are as follows. Effects on pleasure, one of elements of team spirits taken by instructor's training style are analyzed as follows. It was proved to be meaningful in relation with a series of activities like training, democratic, social, compensatory aspects and showed also considerable relation with power based behaviors. That says, players are found to enjoy high pleasure when social and bureaucratic behaviors of instructors are very energetic. In addition to that, training, democratic, and compensatory activities didn't show any meaningful effect. Team spirit was found to play a main role between instructor's behaviors and training, democratic, social rewarding activities. Democratic and social acts influence on team spirit. Looking into the detailed aspects, team spirit was resulted very high in the individuals with low democratic mind and was shown high group spirit by groups with high sociable activities. Teamworks was found to be affected by relation between instructor's acts and training, democratic, social and compensatory aspects and it showed meaningful relations with training, social, bureaucratic behaviors. Low degree of training and bureaucratic activities are found to prefer for power team spirit, and high social activities led a strong teamworks. Group binding spirit was influenced by training, democratic, social compensatory, bureaucratic behaviors and it showed to give effects on democratic, social, and bureaucratic activities of instructors. Low degree of democratic and bureaucratic behaviors are found to produce strong team spirit. In contrast with that, strong social activities was found to be motive of powerful team spirit. Value of team spirit was found to play a main role between instructor's behaviors and training, democratic, social, rewarding activities. It didn't show any meaningful effect on behavior of instructors.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.6
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• pp.140-146
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• 2016

A survey was conducted of the total of 57 adults with disabilities from 60 years old to more than 20 years old residing in Jeju Island in which they were interviewed three times in May 2015. Supplementary data was provided by one of the male adults with disability who acts as a lecturer and helped to improve our awareness of the disabilities. The following conclusions were drawn from this research: First, as regards the effect of the economic activity of the disabled on their self-efficacy, their economic activity does not have any significant effect on their self-regulating efficacy and task difficulty preference, but does have an effect on their self-confidence. Third, according to the $Scheff{\acute{e}}$ verification on the differences in the self-efficacy of the disabled depending on their economic activity, employment (M=23.48, p<.04) and lecturer activities (M=22.06, p<.04) (had a greater effect?) than unemployment (M=18.93, p=<.04) but no difference between their employment and lecturer activities was found.