• 제목/요약/키워드: darkness culture

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A Rhythmicity in the rate of Oxygen Consumption by the Manila clam, Ruditapes philippinarum

  • Kim, Wan-Soo;Rumohr, Heye;Schimid, Michael K.;Koh, Chul-Hwan
    • Journal of the korean society of oceanography
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    • v.31 no.3
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    • pp.117-122
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    • 1996
  • Oxygen consumption by the Manila clam, Ruditapes philippinarum (Bivalvia: Veneridae) maintained in a culture tank for 7-10 weeks was measured with an automatic intermittent-flow-respirometer (AIFR). There was evidence for an endogenous tidal rhythm of 12.4 h in oxygen consumption, at irregular intervals of between about 7 and 18 hours during experiments of between 22 h 5 min and 70 h 32 min (duration) recording for fasting animals in constant darkness at constant temperatures in the absence of tidal rhythms.

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Factors Affecting on Regeneration in 'WHANGKEUMBAE' Pear (Pyrus pyrifolia)

  • Lee, Gung-Pyo
    • Journal of agriculture & life science
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    • v.43 no.6
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    • pp.59-65
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    • 2009
  • Combinations of plant growth regulators, darkness treatments, and the order of expanding leaves for explants were evaluated for optimizing in vitro shoot regeneration rate of 'Whangkeumbae' pear. In a MS medium, supplemented with $8.88{\mu}M$ 6-benzylaminopurine (BA) and $0.49{\mu}M$ indole-3-butyric acid (IBA), green foci were observed on the surface of the callus 8 days after culture initiation. Some adventitious buds were later induced from those green foci, resulting in the appearance of normal shoots. In a medium containing $22.20{\mu}M\;BA$, the surface of the callus became compact and greenish, and many adventitious buds were formed over the entire area of the callus surface. When comparing BA concentration via histological observation, the section which had been treated with $22.20{\mu}M\;BA$ exhibited closer cell aggregation than those with $8.88{\mu}M\;BA$. The darkness treatment enhanced the formation of adventitious shoots for up to 3 weeks. The youngest two expanding leaves, proximal to the shoot apex, were proved to be the most regenerative, and yielded the highest shoot number per regenerating leaf. A fourth strength MS medium, which was supplemented with $0.54{\mu}M\;NAA$, yielded good quality plantlets, with regard to root number and root length.

Embryo Culture of Taxus wallichiana (Zucc.)

  • Datta Mukul Manjari;Jha Sumita
    • Journal of Plant Biotechnology
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    • v.6 no.4
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    • pp.213-219
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    • 2004
  • Zygotic embryos were excised from immature and mature seeds of the Himalayan yew, Taxus wallichiana. The embryos germinated precociously when kept in darkness for 5 weeks and developed into full seedlings within 10-12 weeks. The highest rate of embryo germination ($81\%$) was obtained in modified Lloyd & McCown' s woody plant medium containing macro and micronutrients at half strength supplemented with $1\%$ activated charcoal, which supported both the best embryonic growth ($43\%$) and seedling development ($32\%$). However, the supplementation of basal media with kinetin, thidiazuron, 6-benzyl aminopurine or $GA_3$ had no effect on the germination of the embryos. The embryos derived from immature seeds germinated but the frequency of embryonic growth was better in mature seeds. Stratification of seeds effected precocious germination of embryos. Seeds kept at $4^{\circ}C$ for 1 week germinated earlier and at a higher frequency irrespective of the stage of seed maturity, while the germination rate declined with prolonged cold treatment for 1 month at that same temperature. Analysis of taxanes in germinating seedlings revealed that root tissues contained high levels of taxol, 10-deacetyl-baccatin ill and baccatin ill as compared to shoots. Thus embryo culture technique appears to overcome the lengthy dormancy requirement of T. wallichiana seeds.

Sporulation or Cercospora canescens Ellis & Martin in culture (녹두 갈색무늬병균(Cercospora canescens Ellis & Martin)의 분생포자 형성에 관한 연구)

  • Kwon Shin Han;Oh Jeung Haing
    • Korean journal of applied entomology
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    • v.20 no.1 s.46
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    • pp.21-24
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    • 1981
  • This study was conducted to obtain a supply of conidia sufficient for screening mungbean mutant lines for a source of resistance to Cercospora leaf spot caused by Cercospora canescens Ellis and Martin. Abundant sporulation occurred in cultures on mungbean leaf decoction oatmeal agar(MOA) exposed to about 2,500 Lux of fluorescent light. but it did not occur in continuous darkness. The conditions that produced maximum number of conidia was not coincided with those for vegetative growth and pigmentation in culture medium. Removal of aerial mycelium in culture by brushing with sterile water so enhanced the conidial production that oatmeal agar medium(OA) could be useful for production of abundant conidia by the treatment.

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Induction and Propagation of Protocom-Like Bodies from Shoot Tip Culture in the Pansy Orchid (Miltonia spp.) (경정배양에 의한 밀토니아의 PLB 유기와 기내 증식)

  • Kim, Mi-Seon;Hwang, Sun-Ja;Kim, Jae-Yeong;Choi, I-Jin
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.1
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    • pp.9-14
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    • 2009
  • This study was conducted to find out the commercial in vitro propagation methods through protocom-like bodies (PLB) induced from shoot tip culture of Miltonia spp. Among several culture media for induction PLB from shoot tip in Miltonia spp., MS basal medium was better than Hyponex, Vacin & Went basal medium and other media supplemented with natural additives. PLB's proliferation and differentiation in Hyponex medium including $20g{\cdot}L^{-1}$ banana + $20g{\cdot}L^{-1}$ sucrose(pH 5.2) was better than in MS medium. It was tendency that solid media showed higher PLB fresh weight than liquid medium or other cotton bridge culture. The dark culture for 1~2 weeks and adding $10{\sim}20g{\cdot}L^{-1}$ sucrose on hyponex basal medium was the most effective to increase the PLBs growth and shoot number.

The effect of irradiation and pH on sporulation and growth of Piricularia oryzae CAV. on tomato juice media (Tomato 즙배지의 pH와 조사광선이 도열병균 분생포자생성 및 균사생장에 미치는 영향)

  • Oh S. H.;Cho Y. S.;Lee S. C.
    • Korean journal of applied entomology
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    • v.4
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    • pp.19-24
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    • 1965
  • In an attempt to find a satisfactory environmental factors which facilitate abundant conidial production of Piriculariaoryzae Cav. on tomato juice media, various environmental factors were studied for their effect on sporulation and mycelial growth of the fungus. Those factors were conditions of irradiation, color of light, age of culture and pH of the media. l) Continuous exposure to fluorescent light (Mitsubish FL-20-35 W) produced more conidia and much mycelial growth than did intermittent photoperiods and darkness. 2) Of 3 cellophane filters and direct exposure to fluorescent light used, conidia were produced best under the direct exposure to the light. Conidial production in color filter conditions sequently decreased with red, yellow and blue. Growth of mycelium was not significantly different within colors. 3) Periodic irradiation of 12-hour unit brought about zones on mycelial growth no matter what the color filter was used. 4) Older cultures responding to the light were more stimulated by light than were the younger one in the conidia production, but maximum production of conidia was 48 hours of age in this case. 5) Color of the mycelial mat and the aerial mycelium seemed to have a close relation to the production of conidia. The more darkness of the mycelial mat was produced the more conidia and the much aerial mycelium was produced the least conidia. The color of mycelium was more dark under the continuous irradiation than continuous darkness, while the periodic irradiation showed intermediate effect. 6) The concentration of hydrogen ion for growth and sporulation of the fungus was investigated the ranges between 5 and 9. The best pH for the fungus was also noted at 7. whereas the below of pH 4 was not occurred any mycelial growth and sporulation.

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Sensory Characteristics of Chilled Buckwheat Noodle Soup (mulnaengmyun) (묘사분석을 활용한 시판 물냉면의 평가 기법 확립 및 감각적 특성 분석)

  • Kim, Hyun-Jee;Chung, Seo-Jin;Kim, Miran;Hong, Jaehee
    • Journal of the Korean Society of Food Culture
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    • v.31 no.5
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    • pp.506-514
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    • 2016
  • The objective of this study was to investigate the effect of sample presenting types on the sensory characteristics of chilled buckwheat noodle soup (Naeng-myeon). Generic descriptive analysis was performed for evaluating only stock (system 1), only noodle (system 2) and stock with noodle (system 3). Eight kinds of commercially available Naeng-myeon were samples of interest. Ten female descriptive panelists participated. ANOVA and regression analysis were used for data analysis. In the training sessions, ten sensory properties were developed for system 1, four were additionally established for noodle. In each system, the 8 products showed significantly different intensities in almost all sensory attributes like darkness of stock, overall flavor, sweetness, saltiness, sourness. When integrating the two systems, sample presenting types showed significant difference for the seven sensory attributes, especially saltiness, sourness, beef flavor tended to be rated more strongly in system 1 than system 3.

Comparison Study on Traditional Perceived Meaning of Color and Clothing Color of Korea and Japan (한국.일본의 전통 색채관과 복색에 관한 비교연구)

  • Eum Jung-Sun;Chae Keum-Seok
    • Journal of the Korean Society of Costume
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    • v.56 no.6 s.105
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    • pp.16-32
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    • 2006
  • Perceived meaning of color uniquely forms and is being highlighted as an element of creative design in the modern design industry as well as traditional culture. It is necessary to compare the perceived meanings of color and clothing color of Korea and Japan in order to find out the model of Korea's original color. The purpose of this study Is to draw the results of examining the perceived meanings of color revealed in the culture, arts and clothing color of the both countries and comparing them depending on contemporary times. The scope of study is limited from the ancient times to modern times (about BC.IC-early20C). In the methodologies, the literature and the empirical study focus on both counturies' literature, including art history, ethnology, and the history of clothing; and their paintings and relics, which are all related to clothes. The perceived meaning of color of Korea was prominent with the beauty of nature and gorgeousness throughout the history. The colors were mostly white colors, light colors, and single colors such as obangsaek, which are high pure degree colors by which color is changed depending on darkness and lightness, while that of Japan featured clothing colors combining various colors and middle colors.

Mating Types and Optimum Culture Conditions for Sexual StateFormation of Fusarium fujikuroi Isolates

  • Choi, Hyo-Won;Kim, Jung-Mi;Hong, Sung-Kee;Kim, Wan-Gyu;Chun, Se-Chul;Yu, Seung-Hun
    • Mycobiology
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    • v.37 no.4
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    • pp.247-250
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    • 2009
  • Twenty-five isolates of Fusarium fujikuroi acquired from rice seeds and rice plants evidencing symptoms of Bakanae disease were evaluated to determine their mating types and characterize the formation of their sexual state. The mating types of the isolates were evaluated via multiplex PCR with the diagnostic primers of the mating-type (MAT) region: GFmat1a, GFmat1b, GFmat2c, and GFmat2d. Among the 25 isolates, 11 were identified as MAT-1 (male), and 14 as MAT-2 (female). Four MAT-1 isolates and three MAT-2 isolates were mated and cultured to evaluate the optimal culture conditions for the production of their sexual states. Among four tested media, 10% V8 juice agar proved optimal for the perithecial production of the isolates. The isolates also generated the largest numbers of perithecia when incubated at 23oC in alternating cycles of 12 hr fluorescent light and NUV fluorescent light and 12 hr darkness.

Quality Characteristics of Madeleines Made with the Addition of Roasted Black Soybean Flour (볶음 검정콩가루를 첨가한 마들렌의 품질 특성)

  • Jae-Eun, Jeon;In-Seon, Lee
    • Journal of the Korean Society of Food Culture
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    • v.37 no.6
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    • pp.529-539
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    • 2022
  • This study investigated the quality characteristics of madeleines prepared using varying amounts of roasted black soybean flour (RBF). The RBF was used to substitute 0% (control group), 20% (RBF-20 group), 40% (RBF-40 group) and 60% (RBF-60 group) of weak flour (WF) in the manufacture of madeleine. The substitution of WF with RBF showed decreased the pH but increased the sugar concentration of the batter (p<0.01). Low lightness (L) and low yellowness (b) were observed in the experimental groups at high ratios of RBF substitution (p<0.05). The experimental groups of madeleines showed higher hardness and chewiness than the control group (p<0.001). The principal component analysis of the RBF-60 experimental group, which had the highest proportion of RBF, showed that it had relatively strong characteristics with respect to "darkness", "soybean odor", "sesame odor", "grains odor", "savory flavor", "sweetness", "black soybean taste", and "moistness". The acceptance test results, showed that the RBF-20 experimental group was similar to the control group with respect to "odor acceptance", "taste acceptance", and "texture acceptance". Thus, this study confirmed the possibility of using RBF for the preparation of madeleines.