• Journal of Plant Biotechnology
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• 제50권
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• pp.267-274
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• 2023

All studies on date palm somatic embryogenesis have focused on germination in the presence of light while neglecting germination in darkness, which mimics the germination process of zygotic embryos within seeds. To improve the date palm micropropagation protocol, we investigated the effects of light and darkness incubation on the germination of indirect date palm somatic embryos and their subsequent conversion into plantlets. Darkness incubation emerged as a pivotal factor in the germination of indirect date palm somatic embryos and their successful conversion into plantlets. Darkness incubation significantly decreased the time required for the conversion of indirect somatic embryos into plantlets, halving the duration from 24 weeks to only 12 weeks. The micropropagation protocol was modified, consolidating the previous two distinct stages of germination and elongation under light incubation into a single stage under darkness incubation. These findings modified the protocol and significantly reduced the overall duration of the date palm micropropagation protocol.

• 비교문화연구
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• 제39권
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• pp.153-176
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• 2015

Poet, painter and publisher, Roland $Gigu{\grave{e}}re$ is one of Quebec's outstanding figures, inspired by both Surrealism and Quebec nationalism. He participated in contemporary artistic movement 'Phases' and influenced collective self-awareness and political ferment, 'Quiet Revolution'. In La Main au feu(1973), his poetry represent a landscape dominated by darkness in contrast with red color of fire from the volcanic crater. The world is immersed in darkness of despair which allude to the Great Darkness of Quebec society. Acts of violence assume many different forms: crows, black rain, dark flow, frenzy of knife blows. Both things and humans are in the state of absence or lack. Life falls into opacity of death. In the background of dark landscape, we discover Miror, a singular character. Similar to chain of mountains and to bare forest, he is a creature that shape the tragic inner world of poet. He is as like as seismograph that record the tremble of being. Finally, in order to fight the darkness of environment, the poet attempt to use the power of fire of volcanoes. The flow of magma become paintings of his dream and the flame of eruption, poetry of cry toward the sky. 'La main au feu' means the will to resist injustice and repression in the world. The tragic reality is replaced by a dream that become second reality out of reach of the force of hostile external circumstances.

• 농업과학연구
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• 제2권2호
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• pp.375-380
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• 1975

본 연구는 인공배지(Rice Polish Agar) 위에서 자라는 문고병균(C. sasakii)의 균사생장, 색소형성 및 균핵형성에 미치는 광선의 영향을 구명하기 위하여 배양적 성질이 다른 두균주(D-3, D-15)를 사용하여 실시하였던 바 다음과 같은 결과를 얻었다. 1) 두 균주 모두 연속관선조사구, 광암교호구 및 암처리구에서 균사 생장량에 차이가 없었다 그리고 균주 D-3는 균사 및 배양기가 갈색으로 착색되엇고 암처리구보다 관선조사구에서 더 많이 착색되었으나 균주 D-15에서는 갈색 색소형성이 없었다. 2) 균핵형성에 미치는 광선의 영향은 두 균주간에 차이가 있었다. 즉 균주 D-15는 각 처리간의 총 균핵형성량에는 유의차가 없었으나, 암처리구에서 광암교호구 및 연속 광선조사구 보다 대형균핵이 많이 형성되었다. 반면 균주 D-3는 연속광선조사구에서는 많은 균핵을 광암교호구에서는 중간정도, 암처리구에서는 적은량의 균핵을 형성하였다.

• 복식
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• 제64권2호
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• pp.98-112
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• 2014

Since 2010, Goth styles have risen rapidly in prominence as one of the major trends of fashion. This study intends to understand the Goth style shown from the modern fashion by examining its historically formative characteristics and internal meanings of the Goth style. With mystic attitude toward the medieval Gothic culture, Goth style refers to its reproduction over the cultural circles including music, literature and arts. From the result of the historical review, the formative characteristics of the Goth style may be divided into darkness, distortion, memento mori and bricolage. The historical review of the Goth style contains symbolic meaning of mystery, terror, escape and theatricality. After 2000, Goth style, in combination with factors such as postmodernism and human desire, has been established as the main trend of modern fashion, going with the popular culture. The formativeness of modern Goth fashion is the same as that of Goth style examined from the historic review. However, in modern Goth fashion, the symbolic meaning has the processes of the new: unconsciousness, kitsch, overthrow and popularity.

• 복식문화연구
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• 제14권6호
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• pp.1004-1014
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• 2006

The purposes of this study is to investigate the present condition of nurses' uniform design and to categorize the image of nurses' uniform design. To investigate the nurses' uniform design, total 241 data were collected from the pictures of nurses who work for private hospital and semi-general hospital in Deajeon and Seoul city and Chungnam province and the pictures of uniform for nurses in general hospital presented at nurses' uniform company web sites. This data was evaluated by 3 major garment characteristics, yin/yang, casual/professional, and lightness/ darkness-were used as criteria for categorizing the image of nurses' uniform design. Focus group consisting of 10 experts majored in Clothing and Textiles were evaluated by 7 point Likert type scales. Nurses' uniform design at the present time showed equal distribution in terms of yin/yang and casual/professional. However, in terms of lightness/darkness, nurses' uniform design were partial to light image. Therefore, dark image, specially dark and masculine image in nurses' uniform design were very rare. Nurses' uniform design had some differences by type of hospital, department, and geographical area. The most important meaning from the result through this study was that the nurse uniforms were classified into groups according to the present condition of nurses' uniform design.

• 한국식생활문화학회지
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• 제23권1호
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• pp.121-129
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• 2008

To produce buckwheat sprouts, buckwheats were germinated at $23{\pm}2^{\circ}C$ up to 8 days in total darkness. In proximate analysis, moisture content increased from 13.87% of buckwheat groats to 93.75% of buckwheat sprouts on the 8th day of germination. On dry weight basis, lipid and ash contents increased like as protein content increased from 13.45% to 21.82% while carbohydrate content decreased due to enzyme hydrolysis. Amino acids were rich in glutamic acid, aspartic acids, and lysine, and also the ratio of the essential amino acids to total amino acids increased from 26.84% to 36.84%. Vitamin C did not exist in buckwheat groats but its content has continued to increase as far as 99.56 mg/100 g buckwheat sprouts. Rutin continued to increase by HPLC analysis from 4.71 mg/100 g buckwheat groats to 1,524 mg/100 g buckwheat sprouts. In analysis of organic acids, oxalic, maleic, and citric acids were commonly found in buckwheat sprouts. Fagopyrin was found almost none in buckwheat sprouts. In conclusion, the conversion of buckwheat seeds into sprouts through germination in darkness results in physically different final product with nutritional changes such as higher content of rutin, generation of vitamin C, abundance of the essential amino acids, and the existence of fagopyrin on very little.

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.171-177
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• 2017

우리나라에서 멸종위기 해안식물로 지정된 갯봄맞이의 캘루스배양을 통한 재분화 프로토콜을 보고한다. 마디배양을 통해 육성한 기내 유식물체의 절편은 유전자형, 절편의 종류, 광조건 및 2,4-D 첨가농도에 따라 캘루스 형성에 상이한 반응을 보였다. 특히, 다른 처리와는 무관하게 연속 암배양이 절편으로부터 캘루스 유도에 결정적인 영향을 미쳤다. 2,4-D 0.1-3.0mg/L 농도 범위의 배지에서 유도된 캘루스를 동일한 조성의 배지로 이식하여 암배양했을 때왕성하게 캘루스가 증식하였다. 연속 암조건에서 zeatin 1.0mg/L 첨가배지에 'Pistachio' 유전자형의 캘루스를 배양했을 때만 유일하게 정상적인 신초가 재분화 되었고, 이 때 신초는 캘루스로부터 형성된 마디구조에서 발달하였으며 신초의 재분화율은 24.4%였다. 재분화 신초를 식물호르몬 무첨가 배지로 이식하여 명배양 했을 때 지상부 녹화와 더불어 뿌리가 발생하여 유식물체로 발달하였다. 기내 유식물체를 기 보고된 마디배양 프로토콜(Bae et al. 2016)에 따라 배양했을 때 효과적으로 유식물체를 증식시킬 수 있었다. 기외 순화시킨 유식물체는 내륙 자연 환경하에서 성숙식물체로 생육하였으며 이듬 해 4월에 개화하였다. 본 연구의 결과는 Bae et al. (2016)이 제안한 마디배양 프로토콜과 통합하여 운용될 수 있고, 통합된 프로토콜은 갯봄맞이의 기내 번식 시스템으로서 갯봄맞이 자생지 복원에 기여할 수 있을 것이다.

• Journal of Plant Biology
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• 제2권1호
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• pp.1-12
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• 1959

1) The comparative studies of the quantitative measurement of growth characteristics and utilization of substrates by Euglena gracilis var. bacilla 10616 in the light and in darkness have been carried out. Eodogenous respiration, effect of respiratory inhibitors and responses to the added substrates for the exogenous respiration are also investigated. 2) All cultures are grown in the open air under the continuous illumination of fluorescent light of 3500 lux at room termperature, the growth rate of the culture in the basal medium added 0.5% lactate is found to be the highest. The growth rate decreases successively for the cultures of 0.5% sucinate, 0.5% Na-acetate, 0.5% malate, and control. There is no growth in the basal meidum added 0.5% butyrate and 0.5% hydroquinone. The similar results are obtained for the mentioned cultures in the darkness. However, the growth rate in basal medium added 0.5% glucose and 0.5% sucrose does seem to increase in the darkness unlike the illumination. 3) The endogenous rate of respiration for the organism cultured photosynthetically is about 12.94ul 02/mg/hr, in basal medium and the respiratory quotient is about 0.84. The rate is decreased by starvations to 6.5ul 02/mg/hr, about to a half, but the respiratory quotient does net change. 4) The oxygen consomption during initial 2 hours in suspending solution ranging from pH 4.5 to pH 9.3 is highest at pH 4.5 in which the algae had grown, at pH 5.5 and at pH 6.9. 5) Endogenous respiration of the cells is strongly inhibited by 0.1M of potassium cyanide, malomic acid, sodium fluoride and iodo-acetic acid. It is also strongly inhibited by 0.01M of potassium cyanide. 6) The respiratory response to added substrates for the exogenous respiration in the organism is coincided with the rate in the basal medium added the substrate in light and in darkness, whether the cells are fed or starved. 7) According to the results of this study, there seems to be the flexibility of the interconversion between photosynthesis and chemosynthesis, heterotropic mode of metabolism, in Euglena gracilis var. bacillaris, and that this organism utilizes the lactate most. It also may be suggested that the enayme systems linked in the each steps of Embden-Myerhof-Parnas path way and TCA cycle seem to exist in this organism.

• Journal of Plant Biology
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• 제31권1호
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• pp.41-49
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• 1988

Several cultivars of rice were examined for induction of embryogenic callus on a medium containing MS salts, vitamins and 2, 4-D under darkness. Embryogenic callus was obtained from cultivar Cheonma with high ratio and embryo-like structures were formed from the callus on a medium with or without reduced 2, 4-D. Somatic embryoids with a plumule and radicle axis surrounded by a scutellum were observed. These embryoids germinated and produced plantlets in 30 days on the same medium. Protoplasts isolated from an embryogenic cell suspension culture derived from embryogenic callus were cultured either in liquid or in agar medium and protoplast derived cell colonies were obtained in 3-4 weeks.

• 비교문화연구
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• 제45권
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• pp.479-496
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• 2016

본 논문은 죠셉 콘래드의 "어둠의 속"에서 중심인물인 말로우가 자신의 아프리카 탐험을 바탕으로 한 서사를 통해서 스스로를 서구 계몽주의의 합리적 이성주체 그리고 진리주체로 드러내고자 하는 시도를 하고 있음을 고찰하고자 한다. 작품에서 제국주의 이데올로기와 성 이데올로기는 서구 계몽주의 합리적 이성주체를 구성하는 데 작동하고 있다. 말로우는 아프리카에서 커츠를 만나러 가는 여정과 그와의 만남을 통해 제국과 성의 이데올로기들이 외부 현실세계에서 작용하고 있다는 사실을 알게 되었다고 자부한다. 그러나 말로우는 자신의 현실인식 역시 타자를 억압하고 타자의 정체성을 소멸시키는 서구의 전체주의적 이데올로기 담론에 의해 침윤되어있다는 사실을 모르는 주체이다. 말로우는 서구 계몽주의 주체인 동시에 계몽주의의 이분법적 위계서열을 가치판단의 기준으로 삼는 이성에 의해 지배당하는 식민주체이다. 말로우는 자신이 제국주의의 본질이란 정치적 폭력과 경제적 수탈로 점철되는 현실이고 이것을 깨달은 진정한 계몽주체라고 자랑한다. 그러나 그의 서사담론은 제국주의에서 발견되는 배타적이며 자기중심적 이데올로기와 성 이데올로기로 원주민을 비인간적 존재로 판단하고 여성을 이상화된 세계에 매몰된 존재로 파악하는 자기모순의 오류를 범하고 있다.