• Title/Summary/Keyword: damage starch

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Inhibition Effects of Persicaria amphibia (L.) Delarbre on Oxidative DNA Damage via ATM/Chk2/p53 pathway

  • So-Yeon Han;Hye-Jeong Park;Jeong-Yong Park;Seo-Hyun Yun;Mi-Ji Noh;Soo-Yeon Kim;Tae-Won Jang;Jae-Ho Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.52-52
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    • 2021
  • Persicaria amphibia as an England native plant, is a rhizomatous perennial, one of the rather amphibious plants. Its aquatic form contains water-soluble sugars, starch, and protein. P. amphibia have up to 18% tannins in stems and rhizomes. Previous studies have confirmed the anti-inflammatory activity of live bacteria roots, but no studies on bioactivity are known. DNA damage responses (DDRs) pathways are considered a crucial factor affecting the alleviation of cellular damage. The ataxia-telangiectasia mutated and Rad3 related (ATM) and checkpoint kinase 2 (Chk2) pathways are the main pathways of DNA damage response. Also, p53 is a key integrator of cellular response to oxidative DNA damage, contributing repair, or leading transcription including apoptosis. In the present study, we conducted an investigation into the inhibitory effects of P. amphibia on oxidative DNA damage for confirming potential to complementary medicine and therapies. In conclusion, P. amphibia can provide protective effects against double-stranded DNA break (DSB) caused by oxidative DNA damage.

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Physicochemical characteristics of rice variety for dry-milled flour

  • Yoon, Mi-Ra;Kwak, Jieun;Lee, Jeom-Sig;Won, Yong-Jae;Kim, Mi-Jung;Choi, Induck;Jeon, Yong-Hee;Kim, Sun Lim
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.294-294
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    • 2017
  • Rice (Oryza sativa L.) is one of the main agricultural crops in Asian countries, including Korea, and is considered as the most important staple food in the world. Rice is also processed into flour, which is consumed through various foods such as cake, noodle, bread, and confectionary. Rice flour quality is highly dependent on variety and milling conditions. Producing rice flour with fine particles is more difficult than wheat flour because of its grain hardness. The Korean rice varieties representing different amylose contents were selected for this study. The relationship between the morphological and starch characteristics of rice kernels and the appropriate varieties for producing good-quality, dry-milled rice flour were examined. The hardness of the rice kernels was determined by measuring the pressure at the grain breakage point. The damaged starch content of the rice flour was determined using a Megazyme starch damage assay kit. The particle-size distribution of the rice flour was measured as the volume-base distribution using a laser-diffraction particle size analyzer. The mean particle-size distribution of the dry-milled flour obtained was between $65.3{\sim}105.1{\mu}m$ among the rice varieties. The opaque, non-glutinous, Seolgaeng rice demonstrated a narrow peak at the fine size, whereas the entire particle-distribution range for other varieties was wide. Seolgaeng exhibited significantly lower damaged starch content of dry-milled flour than the other varieties (p < 0.05). Seolgaeng showed lowest in energy consumption on rice flour production with 200 mesh particle size. Accordingly, it is possible to produce dry-milled rice flour which is similar to wheat flour that would considerably reduce milling costs.

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Effect of particle size of naked oat flours on physicochemical and antioxidant property (쌀귀리 가루의 입도별 이화학적 특성 및 항산화 활성)

  • Jun, Hyun-Il;Yoo, Sun-Hee;Song, Geun-Seoup;Kim, Young-Soo
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.965-974
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    • 2017
  • This study was carried out to investigate the effect of particle size of naked oat flour (NOF) on physicochemical property and antioxidant activity. The NOF was passed through $250{\mu}m$ and $160{\mu}m$ size sieves to obtain three fractions (fraction A: $250{\mu}m$ or more, fraction B: $160-250{\mu}m$, and fraction C: $160{\mu}m$ or less). Moisture, crude protein, crude fat, and crude ash contents of NOF were 8.4, 15.7, 10.0, and 1.8%, respectively, and these contents were increased as the particle size of NOF decreased. The mineral and free amino acid contents of NOF also had a similar tendency. The contents of total starch, amylose, starch damage, total dietary fiber, ${\beta}$-glucan, total phenolics, and flavonoids in NOF were 56.4%, 21.4%, 11.7%, 11.0%, 4.7%, $237.8{\mu}g/g$ and $90.9{\mu}g/g$, respectively. As the particle size of NOF decreased, total starch, amylose, and starch damage contents increased, whereas total dietary fiber, ${\beta}$-glucan, total phenolic and flavonoid contents decreased. Also, three antioxidant activities of NOF were closely correlated with their total phenolics and flavonoids contents, showing high correlation coefficient values ($R^2=0.87$ and 0.81, respectively).

A REVIEW OF THE MICROBIAL DIGESTION OF FEED PARTICLES IN THE RUMEN

  • McAllister, T.A.;Bae, H.D.;Yanke, L.J.;Cheng, K.J.;Ha, J.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.7 no.3
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    • pp.303-316
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    • 1994
  • Microbial digestion of feed in the rumen involves a sequential attack culminating in the formation of fermentation products and microbial cells that can be utilized by the host animal. Most feeds are protected by a cuticular layer which is in effect a microbial barrier that must be penetrated or circumvented for digestion to proceed. Microorganisms gain access to digestible inner plant tissues through damage to the cuticle, or via natural cell openings (e.g., stomata) and commence digestion from within the feed particles. Primary colonizing bacteria adhere to specific substrates, divide to form sister cells and the resultant microcolonies release soluble substrates which attract additional microorganisms to the digestion site. These newly attracted microorganisms associate with primary colonizers to form complex multi-species consortia. Within the consortia, microorganisms combine their metabolic activities to produce the diversity of enzymes required to digest complex substrates (e.g., cellulose, starch, protein) which comprise plant tissues. Feed characteristics that inhibit the microbial processes of penetration, colonization and consortia formation can have a profound effect on the rate and extent of feed digestion in the rumen. Strategies such as feed processing or plant breeding which are aimed at manipulating feed digestion must be based on an understanding of these basic microbial processes and their concerted roles in feed digestion in the rumen.

A New α-Amylase from Reticulitermes speratus KMT1

  • Park, Han-Saem;Ham, Youngseok;Ahn, Hee-Hoon;Shin, Keum;Kim, Yeong-Suk;Kim, Tae-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.42 no.2
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    • pp.149-156
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    • 2014
  • Termites are wood pests that cause vast economic damage every year. They digest both cellulose and starch, but the enzymes for starch digestion have not been well characterized. We obtained complete amino acid sequence information on the KME1 ${\alpha}$-amylase from Reticulitermes speratus KMT1 through analysis of total mRNA sequences. The KME1 enzyme has two ${\alpha}$-amylase domains and is 68% identical to the ${\alpha}$-amylase from Blattellager manica, its closest relative in the GenBank database. Some unique features of its conserved region and its distant evolutionary relationship to other insect ${\alpha}$-amylases suggest that KME1 is a new type of ${\alpha}$-amylase.

Change in Physicochemical and Storage Characteristics of Jeungpyun by Addition of Pectin and Alginate powder (펙틴, 알긴산가루를 첨가한 증편의 이화학적 특성과 저장 중 변화)

  • Park, Mie-Ja
    • Korean journal of food and cookery science
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    • v.21 no.6 s.90
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    • pp.782-793
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    • 2005
  • This study investigated the effect of $2\%$ addition of pectin and alginate on the Physicochemical and retrogradation Properties of Jeungpyun, a Korean traditional fermented rice cake. The volume of batters with alginate and Pectin was significantly larger than that of control. Jeungpyun samples with Pectin and alginate had a larger volume with uniform and smaller cell size. All samples showed largest foaming capability after second fermentation. Foaming capability of the control ($0\%$ addition of pectin and alginate) was significantly larger than that of the treated samples. The amount of reducing sugar tended to increase during fermentation but decreased after steaming, which was due to the increase in hydrolysis of starch. On the contrary, the content of free sugar was slightly decreased during fermentation but slightly increased after steaming. The control contained the largest amount of free sugar after steaming. The microstructure of starch particles after fermentation showed completely dispersed starch granules with air bubbles. After steaming, the structure was sponge-like in all samples. Samples with added alginate and pectin had significantly higher water binding capacity than those of the control. All samples showed noticeably increased solubility and swelling power at $70^{circ}C$ with the control being significantly lower than the treated samples. Retrogradation was measured with $\alpha$-amylase and the retrogradation process of the sample with added alginate and pectin proceeded slower than that of the control. The relative crystallinity was observed through X-ray diffraction method and samples with added alginate and pectin had smaller crystallinity and delayed retrogradation compared to the control. Thus, Jeungpyun with the addition of alginate and Pectin demonstrated improved functionality and dietary fiber addition effect. The storage period of was extended as the retrogradation rate was delayed by the addition of dietary fibers.

Starch and Pasting Characteristics of Various Rice Flour Collected from Markets (시중 유통되는 쌀가루의 전분 및 호화 특성)

  • Lee, Na-Young
    • Food Science and Preservation
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    • v.19 no.2
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    • pp.257-262
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    • 2012
  • pasting characteristics. Their water contents, water holding capacities, solubilities, swelling power, amylose contents, damaged starches, particle sizes and pasting characteristics were measured. The water content and water holding capacities of various types of rice flours collected in the market were 7.64-14.97% and 95.22-232.94%, respectively. Their solubilities and swelling power were 1.97-37.40% and 1.33-5.76, respectively. Various types of rice flour had 32.11-305.67 ${\mu}m$ particle sizes. Among the various types of rice flour collected in the market, the lowest starch damaged content was 3.17%. The peak viscosities of the samples were 17.74-295.95 RVA. Among the rice flours that were collected in the market, the lowest pasting temperature was $69.72^{\circ}C$. As a result, it was shown that rice flour types with high starch damage contents tend to have higher expressed water holding capacities. Moreover, the samples with lower particle sizes tended to have final viscosities, and pasting temperatures.

Physicochemical Characteristic of Korean Wheat Semolina (우리밀 Semolina의 이화학적 특성)

  • Kim, Yeon-Ju;Kim, Rae-Young;Park, Jae-Hee;Ju, Jong-Chan;Kim, Won-Tae;Chun, Soon-Sil
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.6
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    • pp.837-842
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    • 2010
  • The physicochemical properties of coarse semolina (CS), medium semolina (MS) and fine semolina (FS) were investigated to research method applied in noodles processing of Korean wheat semolina. Large particle (>250 ${\mu}m$) was over 75% in all semolinas, except for FS, and the particle distribution of MS and durum semolina (DS) was similar. Crude protein and crude ash were the highest in DS followed by CS>MS>FS. Crude lipid of DS was the lowest among samples and CS, MS and FS were not significantly different. L value was high in semolina with small particle distribution and starch damage was the lowest in DS followed by FS>MS. Amylose content was high in DS (29.80%) and FS (29.08%) with small particle distribution. Water binding capacity was the highest in DS, and FS showed the highest water binding capacity among Korean wheat samples. Solubility and swelling power were noticeably high in FS with low starch damage and small particle distribution. In scanning electron microscope (SEM), FS and MS showed distribution of separated fine particles of flours. From these results, the physicochemical properties of semolina showed many differences by grinding methods. FS should be applied in noodles processing through additional examination about characteristic of noodle making.

Indentation Damage of Porous Alumina Ceramice

  • Ha, Jang-Hoon;Lee, Chul-Seung;Kim, Jong-Ho;Kim, Do-Kyung
    • Journal of the Korean Ceramic Society
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    • v.41 no.1
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    • pp.19-23
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    • 2004
  • The Hertzian indentation contact damage behavior of porous alumina with controlled pore shape was investigated by experiments. Porous alumina ceramics containing well-defined pore shape, size and distribution were prepared by incorporation of fugitive spherical starch. Porous alumina with isolated pore structure was prepared with porosity range up to 30%. The indentation stress-strain curves of porous alumina were constructed. Elastic modulus and yield stress can be obtained from the stress-strain relationship. Impulse excitation method for the measurement of elastic modulus was also conducted as well as Hertzian indentation and was confirmed as a useful tool to evaluate the elasticity of highly porous ceramics. Elastic modulus of the inter-connected pore structure is more sensitive to porosity than that of the isolated pore structure. When the specimen had isolated pore structure, higher yield point was obtained than it had inter-connected pore structure. This study proposed that the elastic modiulus of porous ceramics is strongly related to not only porosity, but also the structure of pore.

The Destruction of Bacterial Spores Upon Compressional Pressure (타정 압력에 의한 세균포자의 파괴현상)

  • Lee, Cherl-Ho;Kim, Young-Man;Lee, Jung-Chi;Jung, Pil-Keun
    • Korean Journal of Food Science and Technology
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    • v.12 no.4
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    • pp.272-277
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    • 1980
  • The tolerance of useful bacterial spores to the conditions of tablet making, specifically, the destruction of bacterial spores upon compressional pressure was investigated. The damage of bacterial spores occurred mainly during the tabletting. The bacterial spores obeyed a logarithmic destruction rate upon compressional pressure. The spore destruction rate was dependent upon the strains of microorganism. The Decimal Reduction Pressure, designated as P-value, were $2.9\;ton/cm^2$, $2.6\;ton/cm^2$ and $2.1\;ton/cm^2$ for the spores of Bacillus subtilis, Bacilus coagulans and Clostridium butyricum, respectively, and $1.7\;ton/cm^2$ for the vegetative cell of Streptococcus faecalis. The spore destruction upon compressional pressure was influenced by the type of filler. The P-value of the spore of B. coagulans was $2.8\;ton/cm^2$ in the lactose filler, but $2.0\;ton/cm^2$ in the starch filler. The number of viable spores was inversely proportional to the hardness and density of tablet, in case that the same type of filler was used. The starch filler, which resulted in the lower hardness and lower density of tablet, caused higher spore destruction rate compared with the lactose filler.

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