• Journal of Korean Medicine Rehabilitation
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• v.33 no.2
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• pp.1-18
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• 2023

Objectives The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samul-tang-ga-dansam water extract (SD) in wound-induced mice. Methods The mice were divided into five groups (n=7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group. The normal group had no wounds and the other groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively. Antioxidant and anti-inflammatory protein levels were evaluated using western blot analysis. Skin tissue was analyzed by H&E, Masson's trichrome staining method. Results Oral administration of the SD significantly reduced the visible skin damage and decreased the reactive oxygen species and ONOO^- activity of the serum. It significantly increased heme oxygenase-1, superoxide dismutase, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor α, interleukin (IL)-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22^phox and p47^phox and increased α-smooth muscle actin and COL1A1 protein expression in fibroblasts involved in connective tissue repair. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing. Conclusions It is suggested that Samul-tang-ga-dansam has antioxidant and anti-inflammatory effects and promotes wound tissue repair.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.2
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• pp.105-116
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• 2023

The fatality rate of secondary accidents is seven times that of general traffic accidents. If limited to highways, one in four deaths are said to occur from secondary accidents. Unexpected situations which do not give drivers time to prepare are the cause of secondary accidents. This risk results in more fatalities on highways with high driving speeds. Existing studies have conducted research on traffic accidents and on secondary traffic accidents that occur after a primary traffic accident, without considering unexpected situations that may occur on the road. Therefore, to reduce damage and casualties caused by secondary accidents, there is a need to create a safe road environment by removing the possibility of causing accidents. This study analyzes whether the day of occurrence, time of occurrence, and radius of the curve of an unexpected situation are related to the occurrence of an unexpected situation. This study was based on data of accidents that occurred in 2022 on the Cheonan-Nonsan Expressway and the Seoul-Yangyang Expressway. The radius of the curve was calculated by dividing the section of the highway into straight, clothoid, and curved sections through cluster analysis. Results of the analysis indicate that the day and time of occurrence and the curve radius are associated with unexpected situations.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.5
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• pp.445-455
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• 2023

Sea level rise due to global warming is accelerating. According to the IPCC survey, the expected sea level rise in 2100 was analyzed to be 47cm in the low-carbon scenario (SSP 1-2.6) and 82cm in the high-carbon scenario (SSP 5-8.5). Sea level rise can cause serious damage to port infrastructure and reduce the safety of ships docked inside ports. In this study, Mokpo Port, which frequently suffers from flooding during high tide, was selected and the sensitivity of mooring evaluation factors was analyzed for actual berthing ships according to sea level rise scenarios. From the analysis, we found that the tension of mooring line, the load of bollard, vertical angle of mooring line, and ship's motion of 6-DOF, which are evaluation factors, generally increased when the sea level increased. The most sensitive evaluation factor was sway motion of 6-DOF. Also, we analyzed that the value of mooring evaluation factors decreased when the crown height was raised. This was beneficial in improving ship and pier safety. The results of this study can be used as basic data to secure measures to improve port and ship safety according to sea level rise in Mokpo Port.

• Journal of the Korean Geotechnical Society
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• v.22 no.11
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• pp.123-131
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• 2006

Liquefaction-induced lateral spreading has been the most extensive damage to pile foundations during earthquakes. Several cases of pile failures were reported despite the fact that a large margin of safety factor was employed in their design. In this study, 1-g shaking table tests were performed in order to analyze the failure behavior of piles embedded in liquefied soil deposits by buckling instability. As a result, it can be concluded that the pile subjected to excessive axial loads $(near\;P_{cr})$ can fail easily by buckling instability during liquefaction. When lateral spreading took place in sloping grounds, it was found that lateral loading due to lateral spreading increased lateral deflection of pile and reduced the buckling load. In addition, from the buckling shape of pile, difference between Euler's buckling and pile buckling vat observed. In the case of pile buckling, hinge formed at the middle point of the pile, not at the bottom. And in sloping grounds, location of hinge formation got lower compared with level ground because of the soil movements.

• The Journal of the Convergence on Culture Technology
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• v.8 no.3
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• pp.449-455
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• 2022

The Colony of Selaginella involvens (Sw.) Spring in Sanyang-myeon, Mungyeong-si, can be divided into five types, and in the past, it was said that Selaginella involvens (Sw.) Spring formed colonies throughout the colony and grew naturally, but now it remains only in some sections. It was found that the Selaginella involvens (Sw.) Spring colony was damaged by artificial and natural factors, and as an artificial factor, the Selaginella involvens (Sw.) Spring was proven to be effective for medicinal and cremation, and many people were damaging the Selaginella involvens (Sw.) Spring colony without permission. Naturally, vines thrive and Selaginella involvens (Sw.) Spring die, and the reality is that the entire colony of Selaginella involvens (Sw.) Spring is in danger of being damaged if maintenance is not performed. On the other hand, there are sections that reproduce with symbiosis with some herbaceous plants, so it is necessary to plan and implement conservation strategies. In order to preserve the Selaginella involvens (Sw.) Spring colony, CCTV is needed to prevent artificial damage, and on the contrary, install facilities such as fences can easily burn up and create worse scenery, so it can preserve the good environment, restore recoverable areas, and install supplementary buffer zones.

• Molecules and Cells
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• v.45 no.3
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• pp.134-147
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• 2022

The anti-oxidant enzyme heme oxygenase-1 (HO-1) is known to exert anti-inflammatory effects. From a library of pyrazolo[3,4-d]pyrimidines, we identified a novel compound KKC080096 that upregulated HO-1 at the mRNA and protein levels in microglial BV-2 cells. KKC080096 exhibited anti-inflammatory effects via suppressing nitric oxide, interleukin1β (IL-1β), and iNOS production in lipopolysaccharide (LPS)-challenged cells. It inhibited the phosphorylation of IKK and MAP kinases (p38, JNK, ERK), which trigger inflammatory signaling, and whose activities are inhibited by HO-1. Further, KKC080096 upregulated anti-inflammatory marker (Arg1, YM1, CD206, IL-10, transforming growth factor-β [TGF-β]) expression. In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridinetreated mice, KKC080096 lowered microglial activation, protected the nigral dopaminergic neurons, and nigral damage-associated motor deficits. Next, we elucidated the mechanisms by which KKC080096 upregulated HO-1. KKC080096 induced the phosphorylation of AMPK and its known upstream kinases LKB1 and CaMKKbeta, and pharmacological inhibition of AMPK activity reduced the effects of KKC080096 on HO-1 expression and LPS-induced NO generation, suggesting that KKC080096-induced HO-1 upregulation involves LKB1/AMPK and CaMKKbeta/AMPK pathway activation. Further, KKC080096 caused an increase in cellular Nrf2 level, bound to Keap1 (Nrf2 inhibitor protein) with high affinity, and blocked Keap1-Nrf2 interaction. This Nrf2 activation resulted in concurrent induction of HO-1 and other Nrf2-targeted antioxidant enzymes in BV-2 and in dopaminergic CATH.a cells. These results indicate that KKC080096 is a potential therapeutic for oxidative stress-and inflammation-related neurodegenerative disorders such as Parkinson's disease.

• Journal of Veterinary Science
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• v.23 no.1
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• pp.8.1-8.15
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• 2022

Background: Brucella infection induces brucellosis, a zoonotic disease. The intracellular circulation process and virulence of Brucella mainly depend on its type IV secretion system (T4SS) expressing secretory effectors. Secreted protein BspJ is a nucleomodulin of Brucella that invades the host cell nucleus. BspJ mediates host energy synthesis and apoptosis through interaction with proteins. However, the mechanism of BspJ as it affects the intracellular survival of Brucella remains to be clarified. Objectives: To verify the functions of nucleomodulin BspJ in Brucella's intracellular infection cycles. Methods: Constructed Brucella abortus BspJ gene deletion strain (B. abortus ∆BspJ) and complement strain (B. abortus pBspJ) and studied their roles in the proliferation of Brucella both in vivo and in vitro. Results: BspJ gene deletion reduced the survival and intracellular proliferation of Brucella at the replicating Brucella-containing vacuoles (rBCV) stage. Compared with the parent strain, the colonization ability of the bacteria in mice was significantly reduced, causing less inflammatory infiltration and pathological damage. We also found that the knockout of BspJ altered the secretion of cytokines (interleukin [IL]-6, IL-1β, IL-10, tumor necrosis factor-α, interferon-γ) in host cells and in mice to affect the intracellular survival of Brucella. Conclusions: BspJ is extremely important for the circulatory proliferation of Brucella in the host, and it may be involved in a previously unknown mechanism of Brucella's intracellular survival.

• Journal of the Korean Institute of Gas
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• v.27 no.2
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• pp.7-15
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• 2023

The electric submersible pump (ESP) has been operating in production wells around the world because of its high applicability and operational efficiency among artificial lift techniques. When operating an ESP in a reservoir, variables such as temperature, pressure, gas/oil ratio, and flow rate are factors that affect ESP performance. In particular, free gas in the production fluid is a major factor that reduces the life and operational efficiency of ESP. This study presents the flow loop system which can implement the performance and damage tests of ESP considering field operating conditions to quantitatively analyze the variables that affect ESP performance. The developed apparatus in an integrated system that can diagnose the failure and causes of ESP, and detect leak of tubing by linking ESP and tubing as one system. In this study, the flow conditions for stable operation of ESP were identified through single phase and two phase flow experiments related to evaluation for the performance of ESP. The results provide the basic data to develop the failure prediction and diagnosis program of ESP, and are expected to be used for real-time monitoring for optimal operating conditions and failure diagnosis for ESP operation.

• Journal of Korea Water Resources Association
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• v.55 no.4
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• pp.257-266
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• 2022

Inundation damage is increasing every year due to localized heavy rain and an increase of rainfall exceeding the design frequency. Accordingly, the importance of hydraulic structures for flood control and defense is also increasing. The hydraulic structures are designed according to its purpose and performance, and the amount of flood is an important calculation factor. However, in Korea, design rainfall is used as input data for hydrological analysis for the design of hydraulic structures due to the lack of sufficient data and the lack of reliability of observation data. Accurate probability rainfall and its temporal distribution are important factors to estimate the design rainfall. In practice, the regression equation of temporal distribution for the design rainfall is calculated using the cumulative rainfall percentage of Huff's quartile method. In addition, the 6^th order polynomial regression equation which shows high overall accuracy, is uniformly used. In this study, the optimized regression equation of temporal distribution is derived using the variable selection method according to the principle of parsimony in statistical modeling. The derived regression equation of temporal distribution is verified through the significance test. As a result of this study, it is most appropriate to derive the regression equation of temporal distribution using the stepwise selection method, which has the advantages of both forward selection and backward elimination.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.4D
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• pp.513-521
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• 2008

The objective of this study is the evaluation of the impact on the construction condition due to historical observation data and IPCC SRES A2 climate change scenario. For this purpose, daily precipitation and daily mean temperature data which have been observed over the past 30 years by Korea Meteorological Administration are collected and applied. Also, A2 scenarios during 2011~2040 and 2051~2080 are used for this analysis. According to the results of trend analyses on annual precipitation and annual mean temperature, they are on the increase mostly. The available working day and the day occurred an extreme event are used as correlation indices between climate factor and construction condition. For the past observation data, linear regression and Mann-Kendall test are used to analyze the trend on the correlation index. As a result, both working day and extreme event occurrence day are increased. Likewise, for the future, variation analysis showed the similar result to that of the past and the occurrence frequency of extreme events is increased obviously. Therefore, we can project to increase flood damage potential on the construction site by climate change.