Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Nucleomodulin BspJ as an effector promotes the colonization of Brucella abortus in the host

  • Ma, Zhongchen (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Yu, Shuifa (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Cheng, Kejian (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Miao, Yuhe (Fujian Sunvet Biological Technology Co., Ltd) ;
  • Xu, Yimei (Xinjiang Center for Disease Control and Prevention) ;
  • Hu, Ruirui (College of Life Sciences, Shihezi University) ;
  • Zheng, Wei (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Yi, Jihai (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Zhang, Huan (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Li, Ruirui (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Li, Zhiqiang (College of Biology and Food, Shangqiu Normal University) ;
  • Wang, Yong (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University) ;
  • Chen, Chuangfu (International Research Center for Animal Health Breeding, College of Animal Science and Technology, Shihezi University)
  • Received : 2021.08.09
  • Accepted : 2021.10.04
  • Published : 2022.01.31
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Abstract

Background: Brucella infection induces brucellosis, a zoonotic disease. The intracellular circulation process and virulence of Brucella mainly depend on its type IV secretion system (T4SS) expressing secretory effectors. Secreted protein BspJ is a nucleomodulin of Brucella that invades the host cell nucleus. BspJ mediates host energy synthesis and apoptosis through interaction with proteins. However, the mechanism of BspJ as it affects the intracellular survival of Brucella remains to be clarified. Objectives: To verify the functions of nucleomodulin BspJ in Brucella's intracellular infection cycles. Methods: Constructed Brucella abortus BspJ gene deletion strain (B. abortus ∆BspJ) and complement strain (B. abortus pBspJ) and studied their roles in the proliferation of Brucella both in vivo and in vitro. Results: BspJ gene deletion reduced the survival and intracellular proliferation of Brucella at the replicating Brucella-containing vacuoles (rBCV) stage. Compared with the parent strain, the colonization ability of the bacteria in mice was significantly reduced, causing less inflammatory infiltration and pathological damage. We also found that the knockout of BspJ altered the secretion of cytokines (interleukin [IL]-6, IL-1β, IL-10, tumor necrosis factor-α, interferon-γ) in host cells and in mice to affect the intracellular survival of Brucella. Conclusions: BspJ is extremely important for the circulatory proliferation of Brucella in the host, and it may be involved in a previously unknown mechanism of Brucella's intracellular survival.

Keywords

Acknowledgement

This research was supported by the National Natural Science Foundation of China (32060789, U1803236), Key Scientific Research Foundation of the Colleges and Universities of Henan Province (21A230015), and Key Development and Popularization Foundation of Henan Province (212102310746).

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