• Biomolecules & Therapeutics
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• 제9권2호
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• pp.112-118
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• 2001

The effects of Malloti Cortex Water Extract (MCWE), bergenin (isolated as an active component from MCWE), and acetylbergenin (synthesized from acetylation of bergenin) on the liver fibrosis induced by bile duct ligation (BDL) in rats. We studied hydroxypro1ine (HYP) as a marker of collagen accumulation in the liver, alanine aminotransferase (s-ALT), aspartate aminotransferase (s-AST), and alkaline phosphatase (s-ALP) as serum markers of liver cell damage induced by BDL, MCWE, bergenin, and acetylbergenin decreased towards normal the accumulated levels of HYP in the liver and the elevated serum levels of s-ALT, s-AST and 5-ALP. The results indicate that MCWE, bergenin, and acetylbergenin ameliorated the liver damage induced by BDL in rats.

• Biomolecules & Therapeutics
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• 제27권2호
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• pp.145-151
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• 2019

Methamphetamine (METH) acts strongly on the nervous system and damages neurons and is known to cause neurodegenerative diseases such as Alzheimer's and Parkinson's. Flavonoids, polyphenolic compounds present in green tea, red wine and several fruits exhibit antioxidant properties that protect neurons from oxidative damage and promote neuronal survival. Especially, epicatechin (EC) is a powerful flavonoid with antibacterial, antiviral, antitumor and antimutagenic effects as well as antioxidant effects. We therefore investigated whether EC could prevent METH-induced neurotoxicity using HT22 hippocampal neuronal cells. EC reduced METH-induced cell death of HT22 cells. In addition, we observed that EC abrogated the activation of ERK, p38 and inhibited the expression of CHOP and DR4. EC also reduced METH-induced ROS accumulation and MMP. These results suggest that EC may protect HT22 hippocampal neurons against METH-induced cell death by reducing ER stress and mitochondrial damage.

• Animal cells and systems
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• 제6권3호
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• pp.263-269
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• 2002

The p53 tumor suppressor gene is among the most frequently mutated and studied genes in human cancer, but the mechanisms by which it sur presses tumor formation remain unclear. DNA damage regulates both the protein levels of p53 and its affinity for specific DNA sequences. Stabilization of p53 in response to DNA damage is caused by its dissociation from Mdm2, a downstream target gene of p53 and a protein that targets p53 for degradation in the proteosome. Recent studies have suggested that phosphorylation of human p53 at Ser20 is important for stabilizing p53 in response to DNA damage through disruption of the interaction between Mdm2 and p53. We generated mice with an allele encoding changes at Ser20, known to be essential for p53 accumulation following DNA damage, to enable analyses of p53 stabilization in vivo. Our data showed that the mutant p53 was clearly defective for full stabilization of p53 in response to DNA damage. We concluded that Ser20 phosphorylation is critical for modulating the negative regulation of p53 by Mdm2, probably through phosphorylation-dependent inhibition of p53-Mdm2 interaction in the physiological context.

• 대한기계학회논문집
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• 제9권2호
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• pp.140-149
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• 1985

본 연구는 오오스테나이트계 STS 316 스테인레스강에 대하여 온도 550.deg. C의 대 기중에서, 변형율제어에 의한 인장-압축에 크리이프 유지시간을 갖는 고온저사이클 피 로시험을 하여 변형율폭 및 크리이프 유지시간이 피로수명에 미치는 영향과 파단면을 주사형 전자현미경으로 관찰하여 크리이프-피로 상호작용이 피로파단면에 미치는 크리 이프 효과를 실험 고찰하였다.

• 비파괴검사학회지
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• 제21권4호
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• pp.425-433
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• 2001

복합재료-금속 접착접합부가 사용 중 반복 하중을 받을 때 발생하는 피로 손상도를 음향초음파(acousto-ultrasonics; AU)법과 음향방출(acoustic emission; AE)법을 이용하여 평가하였다. 피로시험에는 단일겹치기(single-lap) 시험편을 사용하였으며, AU법을 통해 취득한 신호로부터 음향초음파변수(acousto-ultrasonic parameters; AUP)와 피로손상과의 상관관계 곡선을 얻고, AE법에서는 누적 AE events를 통한 피로손상과의 상관관계 곡선을 얻어, AU법과 AE법의 결과를 비교하였다. 이 곡선들은 피로손상에 의한 고분자기지 복합재료의 강성 저하$(E/E_0)$를 나타내는 곡선과 매우 유사하며, 이를 바탕으로 피로 손상도의 예측과 잔여 수명의 예측이 가능하다. 또한 피로 하중의 초기 단계와 피로손상의 누적에 의해 급격한 변화가 나타나는 단계의 파형 주파수 성분을 비교하여, 피로 파괴의 마지막 단계에서 나타나는 신호 중에는 본격적인 피로손상에 의해 발생하는 AE 신호 성분이 포함되어 있음을 확인할 수 있었다.

• 대한토목학회논문집
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• 제43권4호
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• pp.511-523
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• 2023

철근콘크리트 손상 감지를 위한 무인항공기와 딥러닝 연계에 대한 연구가 활발히 진행 중이다. 컨볼루션 신경망은 객체 분류, 검출, 분할 모델의 백본으로 모델 성능에 높은 영향을 준다. 사전학습 컨볼루션 신경망인 모바일넷은 적은 연산량으로 충분한 정확도가 확보 될 수 있어 무인항공기 기반 실시간 손상 감지 백본으로 효율적이다. 바닐라 컨볼루션 신경망과 모바일넷을 분석 한 결과 모바일넷이 바닐라 컨볼루션 신경망의 15.9~22.9% 수준의 낮은 연산량으로도 6.0~9.0% 높은 검증 정확도를 가지는 것으로 평가되었다. 모바일넷V2, 모바일넷V3Large, 모바일넷 V3Small은 거의 동일한 최대 검증 정확도를 가지는 것으로 나타났으며 모바일넷의 철근콘트리트 손상 이미지 특성 추출 최적 조건은 옵티마이저 RMSprop, 드롭아웃 미적용, 평균풀링인 것으로 분석되었다. 본 연구에서 도출된 모바일넷V2 기반 7가지 손상 감지 최대 검증 정확도 75.49%는 이미지 축적과 지속적 학습으로 향상 될 수 있다.

• Journal of Microbiology and Biotechnology
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• 제19권10호
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• pp.1122-1126
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• 2009

The exogenously added cadaverine is effective in protecting Vibrio vulnificus from methyl viologen (MV)-induced superoxide stress at pH 8.5. Such a protective effect by cadaverine was not observed at pH 7.5. Consistently, the accumulated level of intracellular cadaverine at pH 8.5 is approximately four times as much as that of the control cell at pH 7.5. Cadaverine accumulation is not affected by MV. The protection of V. vulnificus by cadaverine from superoxide stress was abolished when cadB coding for the lysine-cadaverine antiporter was interrupted. However, the cadaverine-mediated protection was complemented with cadB DNA. Therefore, CadB of V. vulnificus not only acts as a lysine-cadaverine antiporter at acid pH to neutralize the external medium, but also mediates cadaverine uptake at alkaline pH to result in cell protection from superoxide stress.

• 대한토목학회논문집
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• 제14권5호
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• pp.1097-1104
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• 1994

구조물은 그 수명기간동안 여러 하중을 받게 되고 이로 인해 손상을 입을 수 있으며 때로는 극심한 외부하중에 대해 구조물이 제기능을 상실하거나 파괴에 도달하게 되므로 구조물 설계과정에서 이에 대한 안전성확보가 중요한 요건이 된다. 지진하중을 받는 철근 콘크리트 구조물은 비선형 이력거동을 하게 되며 심한손상을 받을 수 있다. 본 연구에서는 지진하중을 받는 구조물의 추계적 응답특성을 이용하여 손상에 대한 확률적 누적방법을 제안하고 지진하중에 대한 구조물의 손상상수준을 손상지수로서 표현하여 안전성평가에 이용하고자 하였다.

• Advances in concrete construction
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• 제9권5호
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• pp.469-478
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• 2020

This study aims to establish a new methodological framework for the evaluation of the evolution of the reliability of plain concrete for pavement vs number of cycles under flexural fatigue loading. According to the framework, a new method calculating the reliability was proposed through probability simulation in order to describe a random accumulation of fatigue damage, which combines reliability theory, one-to-one probability density functions transformation technique, cumulative fatigue damage theory and Weibull distribution theory. Then the statistical analysis of flexural fatigue performance of cement concrete tested was carried out utilizing Weibull distribution. Ultimately, the reliability for the tested cement concrete was obtained by the proposed method. Results indicate that the stochastic evolution behavior of concrete materials under fatigue loading can be captured by the established framework. The flexural fatigue life data of concrete at different stress levels is well described utilizing the two-parameter Weibull distribution. The evolution of reliability for concrete materials tested in this study develops by three stages and may corresponds to develop stages of cracking. The proposed method may also be available for the analysis of degradation behaviors under non-fatigue conditions.

• Wind and Structures
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• 제31권3호
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• pp.217-227
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• 2020

Based on translation models, both Gaussian and non-Gaussian wind fields are generated using spectral representation method for investigating the influence of non-Gaussian characteristics and directivity effect of wind load on fatigue damage of wind turbine. Using the blade aerodynamic model and multi-body dynamics, dynamic responses are calculated. Using linear damage accumulation theory and linear crack propagation theory, crack initiation life and crack propagation life are discussed with consideration of the joint probability density distribution of the wind direction and mean wind speed in detail. The result shows that non-Gaussian characteristics of wind load have less influence on fatigue life of wind turbine in the area with smaller annual mean wind speeds. Whereas, the influence becomes significant with the increase of the annual mean wind speed. When the annual mean wind speeds are 7 m/s and 9 m/s at hub height of 90 m, the crack initiation lives under softening non-Gaussian wind decrease by 10% compared with Gaussian wind fields or at higher hub height. The study indicates that the consideration of the influence of softening non-Gaussian characteristics of wind inflows can significantly decrease the fatigue life, and, if neglected, it can result in non-conservative fatigue life estimates for the areas with higher annual mean wind speeds.