Browse > Article
http://dx.doi.org/10.4014/jmb.0903.03012

Cadaverine is Transported into Vibrio vulnificus Through its CadB in Alkaline Environment  

Kang, In-Hye (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University)
Kim, Eui-Jin (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University)
Lee, Jeong-K. (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.10, 2009 , pp. 1122-1126 More about this Journal
Abstract
The exogenously added cadaverine is effective in protecting Vibrio vulnificus from methyl viologen (MV)-induced superoxide stress at pH 8.5. Such a protective effect by cadaverine was not observed at pH 7.5. Consistently, the accumulated level of intracellular cadaverine at pH 8.5 is approximately four times as much as that of the control cell at pH 7.5. Cadaverine accumulation is not affected by MV. The protection of V. vulnificus by cadaverine from superoxide stress was abolished when cadB coding for the lysine-cadaverine antiporter was interrupted. However, the cadaverine-mediated protection was complemented with cadB DNA. Therefore, CadB of V. vulnificus not only acts as a lysine-cadaverine antiporter at acid pH to neutralize the external medium, but also mediates cadaverine uptake at alkaline pH to result in cell protection from superoxide stress.
Keywords
Vibrio vulnificus; CadB; cadaverine uptake; alkaline pH; superoxide stress;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Kang, I.-H., J.-S. Kim, E.-J. Kim, and J. K. Lee. 2007. Cadaverine protects Vibrio vulnificus from superoxide stress. J. Microbiol. Biotechnol. 17: 176-179   PUBMED   ScienceOn
2 Lemonnier, M. and L. David. 1998. Expression of the second lysine decarboxylase gene of Escherichia coli. Microbiology 144: 751-760   DOI   PUBMED   ScienceOn
3 Milton, D. L., R. O'Toole, P. Horstedt, and H. Wolf-Watz. 1996. Flagellin A is essential for the virulence of Vibrio anguillarum. J. Bacteriol. 178: 1310-1319
4 Rhee, J. E., H.-M. Ju, U. Park, B. C. Park, and S. H. Choi. 2004. Identification of the Vibrio vulnificus cadC and evaluation of its role in acid tolerance. J. Microbiol. Biotechnol. 14: 1093-1098   ScienceOn
5 Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y
6 Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmid for DNA cloning in Gramnegative bacteria. Gene 70: 191-197   DOI   ScienceOn
7 Kim, J.-S., M.-H. Sung, D.-H. Kho, and J. K. Lee. 2005. Induction of manganese-containing superoxide dismutase in acidic environment is required for the acid tolerance of Vibrio vulnificus. J. Bacteriol. 187: 5984-5995   DOI   ScienceOn
8 Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580   DOI   PUBMED
9 Rhee, J. E., K. S. Kim, and S. H. Choi. 2005. CadC activates pH-dependent expression of the Vibrio vulnificus cadBA operon at a distance through direct binding to an upstream region. J. Bacteriol. 187: 7870-7875   DOI   ScienceOn
10 Ha, H. C., D. J. P. Yager, P. A. Woster, and R. A. Casero Jr. 1998. Structural specificity of polyamines and polyamine analogues in the protection of DNA from strand breaks induced by reactive oxygen species. Biochem. Biophys. Res. Commun. 244: 298-303   DOI   ScienceOn
11 Simon, R., U. Priefer, and A. Puhler. 1983. A broad host mobilization system for in vivo genetic engineering: Transposon mutagenesis in Gram-negative bacteria. Bio/Technology 1: 37-45
12 Chattopadhyay, M. K., C. W. Tabor, and H. Tabor. 2003. Polyamines protect Escherichia coli cells from the toxic effect of oxygen. Proc. Natl. Acad. Sci. U.S.A. 100: 2261-2265   DOI   ScienceOn
13 Markwell, M. A., S. M. Haas, L. L. Bieber, and N. E. Tolbert. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87: 206-210   DOI   ScienceOn
14 Neely, M. N. and E. R. Olson. 1996. Kinetics of expression of the Escherichia coli cad operon as a function of pH and lysine. J. Bacteriol. 178: 5522-5228
15 Kim, J.-S., S. H. Choi, and J. K. Lee. 2006. Lysine decarboxylase expression of Vibrio vulnificus is induced by SoxR in response to superoxide stress. J. Bacteriol. 188: 8586-8592   DOI   ScienceOn
16 Lee, H.-J., K.-J. Park, A. Y. Lee, S. G. Park, B. C. Park, K.-H. Lee, and S.-J. Park. 2003. Regulation of fur expression by RpoS and Fur in Vibrio vulnificus. J. Bacteriol. 185: 5891-5896   DOI   ScienceOn
17 Merrell, D. S. and A. Camilli. 1999. The cadA gene of Vibrio cholerae is induced during infection and plays a role in acid tolerance. Mol. Microbiol. 34: 836-849   DOI   ScienceOn
18 Merrell, D. S. and A. Camilli. 2000. Regulation of Vibrio cholerae genes required for acid tolerance by a member of the "ToxR-like" family of transcriptional regulators. J. Bacteriol. 182: 5342-5350   DOI   ScienceOn
19 Soksawatmaekhin, W., A. Kuraishi, K. Sakata, K. Kashiwagi, and K. Igarashi. 2004. Excretion and uptake of cadaverine by CadB and its physiological functions in Escherichia coli. Mol. Microbiol. 51: 1401-1412   DOI   ScienceOn
20 Minton, K. W., H. Tabor, and C. W. Tabor. 1990. Paraquat toxicity is increased in Escherichia coli defective in the synthesis of polyamines. Proc. Natl. Acad. Sci. U.S.A. 87:2851-2855   DOI   ScienceOn
21 Tkachenko, A., L. Nesterova, and M. Pshenichnov. 2001. The role of the natural polyamine putrescine in defense against oxidative stress in Escherichia coli. Arch. Microbiol. 176: 155-157   DOI   ScienceOn
22 Rhee, J. E., J. H. Rhee, P. Y. Ryu, and S. H. Choi. 2002. Identification of the cadBA operon from Vibrio vulnificus and its influence on survival to acid stress. FEMS Microbiol. Lett. 208: 245-251   DOI   ScienceOn
23 Soksawatmaekhin, W., T. Uemura, N. Fukiwake, K. Kashiwagi, and K. Igarashi. 2006. Identification of the cadaverine recognition site on the cadaverine-lysine antiporter CadB. J. Biol. Chem. 281: 29213-29220   DOI   ScienceOn