• Title/Summary/Keyword: cytotoxic activities

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Cytotoxic Activity of Medicinal Plant Extracts against Human Tumor Cell Lines

  • Jeong, In-Hong;Lee, Jeong-Ock;Kim, Choul-Soo;Kim, Soo-Un;Ahn, Young-Joon
    • Journal of Applied Biological Chemistry
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    • v.43 no.1
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    • pp.59-61
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    • 2000
  • The cytotoxic activities of the methanol extracts of 44 plant species in 31 families against five human solid A549 (lung), SK-OV-2 (ovarian), SK-MEL-2 (melanoma), XF-498 (central nervous system), and HCT-15 (colon) tumor cell lines were examined using the sulforhodamine B (SRB) assay. Responses varied with both cell line and plant species used. Potent cytotoxic activities ($ED_{50}$, <$40{\mu}g/ml$) against all model tumor cell lines were produced from the extracts of Rhus chinensis gall (Galla rhois), Betula platyphylla var. japonica bark, Inula helenium root, Cinnamomum cassia bark, Cinnamomum sieboldii root bark, Lysimachia davurica whole plant, and Evodia rutaecarpa fruit. These plants may be useful for developing new types of naturally occurring anti-tumor agents.

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A New Cytotoxic Compound from Methanol Extract of Koordersiodendron pinnatum Merr. Leaves

  • Fajriah, Sofa;Megawati, Megawati;Darmawan, Akhmad;Lotulung, Puspa Dewi N.;Salahuddin, Salahuddin;Hanafi, Muhammad
    • Natural Product Sciences
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    • v.26 no.4
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    • pp.279-282
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    • 2020
  • Chemical investigation of the methanol extract of Koordersiodendron pinnatum Merr. leaves resulted a new naphthalene derivative, (Z)-4-(tetradec-3-enyl)naphthalene-1,2,7-triol (1), together with three known compounds, ��-sitosterol (2), 20-epibryonolic acid (3), and scopoletin (4). The structure of the new compound was elucidated based on spectroscopic evidence. The isolated compounds (1-4) were tested their cytotoxic activities against the P-388 murine leukemia cell line and compound 1 has highest activity with IC50 1.94 μM.

Antioxidant and cytotoxic activities of curcumin and its analogs: An exploration of structure-activity relationships (Curcumin과 관련 성분들의 산화방지활성과 세포독성 분석 및 구조와 활성 연관성 조사)

  • Lee, Bo-Hyun;Kim, Hee Jeong;Hong, Jungil
    • Korean Journal of Food Science and Technology
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    • v.53 no.4
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    • pp.463-469
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    • 2021
  • Bioactivities of curcumin, a major pigment of Curcuma longa L., have been widely investigated. In this study, the antioxidant and cytotoxic properties of curcumin and its analogs including ferulic acid, dibenzoylmethane (DBM), and tetrahydrocurcumin (THC), and their structure-activity relationships were assessed. Ferulic acid, THC, and curcumin showed strong scavenging activities against several radicals and exhibited considerable lipid peroxidation inhibitory activity. Curcumin showed the strongest cytotoxic activities against HeLa, HCT-116, IEC-6 and INT 407 cells, whereas ferulic acid did not show any cytotoxic effect up to 100 µM against these cell lines. Cytotoxicity of curcumin and THC was significantly enhanced by superoxide dismutase and diminished by N-acetylcysteine. The combination treatment of curcumin and ferulic acid enhanced the cytotoxicity, whereas the combination of curcumin and DBM offset their toxicity. These results suggest that methoxy phenolic and β-diketon moieties are crucial for the antioxidant- and cytototoxic activities of curcumin, respectively.

Biological Evaluation of Korean Medicinal Plants II

  • Woo, Won-Sick;Lee, Eun-Bang;Chang, Il-moo
    • YAKHAK HOEJI
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    • v.21 no.4
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    • pp.177-183
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    • 1977
  • Extracts of seventy-two Korean plants were evaluated for their biological activities such as antitumor activities against sarcoma 180, leukemia SN and Ehrlich ascites carcinoma, cytotoxic activities, antimicrobial activities and behavoral observations, and the results are tabulated.

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Lupane Derivatives-I: Synthesis and Cytotoxic Activity (루판 유도체의 합성 및 세포독성-I)

  • You, Young-Jae;Kim, Yong;Ahn, Byung-Zun
    • YAKHAK HOEJI
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    • v.46 no.5
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    • pp.295-300
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    • 2002
  • To observe the structure-activity relationship of lupane derivatives both on cytotoxic and antiangiogenic activity, twelve lupane derivatives were prepared; their antiangiogenic and cytotoxic activities were evaluated. Among them, four compounds were more cytotoxic than betulinic acid. Carboxylic acid at C28 seemed to be essential for cytotoxic activity. But, a selective cytotoxicity toward SK-MEL-2 was not observed. As for antiangiogenic activity, none of the compounds except lupeol showed antiangiogenic activity at 30 $\mu\textrm{g}$/mL.

Cytotoxicity of Daucus Carota L. on Various Cancer Cells (인체 암세포에 대한 당근 추출 성분의 세포독성효과)

  • 배송자;한은주;노승배
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.29 no.1
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    • pp.153-160
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    • 2000
  • We investigated the cytotoxic effects of roots and seeds Daucus carota L. on HepG2, HeLa, MCF7, SW626, C6 and NB41A3 cell lines by the MTT assay. Among extracts, the ethylacetate partition layer (DCMEA) of root of Daucus carota L. showed the strongest cytotoxic effects on HepG2, HeLa, C6 and NB41A3 cell lines. On the other hand, methanol(DCM), n-ethylacetate(DCMEA) and n-butanol(DCMB) extract of the seeds of Daucus carota L. also showed significant cytotoxic activities for all six cell lines. $\beta$-carotene, a well-known main component of Daucus carota L. was also tested for its cytotoxic effect. However, in all six cell lines, $\beta$-carotene falied to show significant cytotoxicity. Therefore, the anticancer effect of DCMEA of root fo Daucus carota L. and DCM, DCMH, DCMEA and DCMB extracts of seeds may be caused by components other than $\beta$-carotene. Futher studies are under way to isolate the compounds responsible for the significant cytotoxic activity.

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Cytotoxic Lactones from the Pericarps of Litsea japonica

  • Ngo, Quynh-Mai Thi;Cao, Thao Quyen;Woo, Mi Hee;Min, Byung Sun
    • Natural Product Sciences
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    • v.25 no.1
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    • pp.23-27
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    • 2019
  • From the pericarps of Litsea japonica (Thunb.) Jussieu, eighteen butanolide derivatives (1 - 18) were evaluated for their cytotoxic activity against HeLa, HL-60, and MCF-7 cells. Compounds 1-9 with 2-alkylidene-3-hydroxy-4-methylbutanolides structure exhibited cytotoxic activities against cancer-cell lines. Among them, compound 8 (litsenolide $D_2$) exhibited the most potent cytotoxicity against the tested cell lines, including HeLa, HL-60, and MCF-7, with $IC_{50}$ values of $17.6{\pm}1.3$, $4.2{\pm}0.2$, and $12.8{\pm}0.0{\mu}M$, respectively. Compound 8 induced apoptosis in a dose-dependent manner. Annexin V/Propidium Iodide (PI) double staining confirmed that 8 effectively induced apoptosis in MCF-7 cells. To the best of our knowledge, we have reported cytotoxic activity of butanolides from L. japonica against these cancer-cell lines for the first time.

Antinociceptive, antidiarrhoeal and cytotoxic activity of Aegiceras corniculatum

  • Ahmed, F;Mamun, AH AI;Shahid, IZ;Rahman, AA;Sadhu, SK
    • Advances in Traditional Medicine
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    • v.7 no.2
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    • pp.191-196
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    • 2007
  • The ethanol extract of leaves of the mangrove Aegiceras corniculatum Blanco (Myrsinaceae) was screened for its antinociceptive, antidiarrhoeal and cytotoxic activities. The extract produced significant writhing inhibition in acetic acid-induced writhing in mice at the oral dose of 250 and 500 mg/kg body weight (P < 0.001), which was comparable to the standard drug diclofenac sodium at the dose of 25 mg/kg of body weight. When tested for its antidiarrhoeal effects on castor oil induced diarrhoea in mice, it increased mean latent period and decreased the frequency of defecation significantly at the oral dose of 500 mg/kg body weight (P<0.05; P<0.01) comparable to the standard drug loperamide at the dose of 50 mg/kg of body weight. Moreover, when tested for toxicity using brine shrimp, the extract showed potent activity against the brine shrimp Artemia salina ($LC_{50}$ 10 mg/ml). The overall results tend to suggest the antinociceptive, antidiarrhoeal and cytotoxic activities of the extract.

Pharmacological profiles of Holarhena antidysenterica (L.) Wall bark

  • Rahman, Shafiur;Ali, Eunus;Haque, Rubyat
    • Advances in Traditional Medicine
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    • v.7 no.2
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    • pp.157-161
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    • 2007
  • We undertook the present study to evaluate different pharmacological as antioxidant, antibacterial and cytotoxic activities of the crude ethanolic extract of the stem bark of Holarhena antidysenterica (Family: Apocynaceae). The antioxidant property of the extract was assessed by 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay. The extract showed antioxidant activity ($IC_{50}$ about ${\sim}08$ ${\mu}g/ml$), which was comparable to standard drug ascorbic acid ($IC_{50}$ about ${\sim}10$ ${\mu}g/ml$). The extract showed a broad spectrum of antibacterial activity against all tested gram positive and gram negative bacteria most prominent against Enterococci, Staphylococcus pyogenase and Shigella sonnie. And the zones of inhibitions were ranging from 10 - 21 mm for all the tested bacteria. Its cytotoxic property was evaluated by brine shrimp lethality bioassay. The extract showed significant lethality and the $LC_{50}$, $LC_{90}$ values were 80 ${\mu}g/ml$, 320 ${\mu}g/ml$ respectively. The results tend to suggest that the extract might possess chemical constituent(s) that are responsible for antioxidant, antibacterial and cytotoxic activities.

Screening of Cytotoxicity and Antimicrobial Effects of Hexane Extracts from Cornis fructus (산수유 헥산 추출물의 항균효과 및 세포독성)

  • Chun Hyun Ja;Choi Won Hyung;Lee Jeong Ho;Yang Hyun Ok;Baek Seung Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.17 no.2
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    • pp.476-480
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    • 2003
  • Cornis fructus was extracted by successive extraction and then fractionated with hexane extract to get active fractions. This study was performed to determine the cytotoxic effect of hexane extract from Cornis fructus on NIH 3T3 fibroblasts and cancer cell lines using MTT assay. Hexane extract showed cytotoxic effect against A549, B16 melanoma and MDA-MB-231. Futher fractionation with hexane extract was performed to obtain effective fraction, fraction 3 showed the cytotoxic effect against A549 and MDA-MB-231. In antimicrobial test of each fraction of hexane extract, fraction 5 showed antimicrobial activities against P. putida and P. aeruginosa.