• ALGAE
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• 제36권1호
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• pp.1-12
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• 2021

As the global demand for the carrageenophyte Kappaphycus is steadily increasing, its overall productivity, carrageenan quality, and disease resistance are gradually declining. In the face of this dilemma, wild Kappaphycus populations are viewed as sources of new cultivars that could potentially enhance production; therefore, assessment of their diversity is crucial. This study highlights the morphological and genetic diversity of wild Kappaphycus species obtained from two sites in the Philippines. Nucleotide alignments of available 5' region of the mitochondrial cytochrome c oxidase subunit I (COI-5P) and cox2-3 spacer sequences of Kappaphycus confirmed the presence of K. alvarezii in Guiuan, Eastern Samar and K. striatus in Bolinao, Pangasinan. Based on the concatenated sequences of the COI-5P and the cox2-3 spacer, nine novel haplotypes were observed along with other published haplotypes. However, there was no relationship between haplotype and morphology. These newly recognized haplotypes indicate a reservoir of unutilized wild genotypes in the Philippines, which could be taken advantage of in developing new cultivars with superior traits. DNA barcodes generated from this study effectively expand the existing databank of Kappaphycus sequences and can provide insights in elucidating the genetic diversity of Kappaphycus species in the country.

• 한국어류학회지
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• 제35권1호
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• pp.57-63
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• 2023

본 연구에서는 2018년 2~3월 제주도 남부해역에서 Bathylagidae에 속하는 Lipolagus ochotensis 자어 4개체(체장 13.4~21.3 mm)를 봉고네트로 채집하였다. L. ochotensis 자어는 몸이 길게 신장되었으며, 체고는 낮고, 눈이 돌출되며, 몸의 후반부에 흑색소포가 나 있고, 등지느러미가 몸 중앙에 위치하는 특징을 가진다. 미토콘드리아 DNA COI의 염기서열 625 bp를 분석한 결과, L. ochotensis 성어와 97.6%로 매우 가깝게 나타났다. 국내 처음 보고되는 본 종의 새로운 과명으로 "심해빙어과", 속명으로 "검은빙어속", 국명으로 "검은뺨빙어"를 각각 제안한다.

• 한국환경과학회지
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• 제33권1호
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• pp.87-95
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• 2024

The giant jellyfish, Nemopilema nomurai, is an endemic species found in Northeast Asian waters and their population structures, such as size and genetics, and their environmental characteristics were investigated. N. nomurai was obtained from the Yellow and Northern East China Seas during the summers of 2006, 2007, and 2009. In the northern Yellow Sea, small-sized jellyfish were found to be dominant and towards the southern seas, the size of the jellyfish increased. In the northern East China Sea, only one mode of jellyfish was found in May, and the number of modes increased up-to five in July. However, at the center of the Yellow Sea, one or two modes were found in July, 2007. Thus, different jellyfish populations were present in the northern East China Sea and the Yellow Sea. However, based on first appearance and a cohort analysis using the bell diameter, the jellyfish population in the northern Yellow Sea might be recognized as a distinct group that differed from those found in the northern East China Sea. Furthermore, mitochondrial DNA sequences (cytochrome c oxidase subunit I) of N. nomurai were, determined and compared with genetic structures obtained from jellyfish in the Yellow Sea. The genetic diversity of N. nomurai was highest in the regions around the northern East China Sea and at the center of the Yellow Sea and was the lowest around the northern Yellow Sea. Thus, N. nomurai populations in the Yellow Sea and northern East China Sea might be different concerning their seeding places.

• 한국어류학회지
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• 제35권4호
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• pp.244-252
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• 2023

본 연구는 한국산 병어 및 덕대의 분류학적 위치를 명확히 하기 위해 한국, 중국 및 일본산 병어속(Pampus) 어류의 형태 및 분자 특징을 비교하였다. 한국산 병어는 후두부의 파상 무늬가 가슴지느러미 너머로 뻗어 있고, 새개부 아래쪽 가장자리 홈이 없고, 척추골수가 34개인 점에서 Pampus punctatissimus로 동정되었다. 한국산 덕대는 후두부의 파상 무늬가 가슴지느러미 앞끝까지 도달하지 못하고, 새개부 아래쪽 가장자리 홈이 아래턱 배쪽까지 뻗어 있고, 척추골수가 38~42개인 점에서 Pampus argenteus로 동정되었다. 또한 한국산 병어는 일본산 P. punctatissimus와 평균 0.1% 차이를 보였고, 한국산 덕대는 중국산 P. argenteus와 평균 0.3% 차이를 보였다. 본 연구 결과는 한국산 병어를 P. punctatissimus로, 한국산 덕대를 P. argenteus로 사용하는 것이 타당함을 시사한다.

• 한국어류학회지
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• 제32권4호
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• pp.199-209
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• 2020

본 연구는 예전부터 혼란스러웠던 한국산 대주둥치속, Macroramphosus 어류의 분류학적 위치를 명확히 하기 위해, 한국산 18개체를 일본/대만산 35개체 및 지중해산 M. scolopax와 형태 및 분자 변이를 비교 분석하였다. 한국, 일본 및 대만산 대주둥치속 어류는 제1등지느러미 극조 길이(A-type은 22.8~32.1%, B-type은 15.6~21.4%), 제1등지느러미와 제2등지느러미 사이 길이(A-type은 6.4~9.7%, B-type은 8.6~13.3%), 체고(A-type은 20.0~28.0%, B-type은 17.3~22.6%)에서 두 type으로 명확히 구분되었으나, 유전적으로는 구분되지 않았다(CR에서 0.0~3.3%, cyt b에서 0.0~1.3%, COI에서 0.0~0.5%). 한편, 한국산 대주둥치는 지중해산 M. scolopax와 유전적으로 명확히 구분되어(CR에서 9.9~11.5%, cyt b에서 3.8~4.6%, COI에서 1.2~3.6%), 최근 사용하고 있는 학명 M. scolopax를 M. japonicus (및/또는 M. sagifue)로 변경해야 할 것이다. 그러나, 본 연구에서 두 type 간 형태변이와 분자변이 간 일치성을 찾지 못했으며, 이는 아마도 그들간에 분화가 상당히 최근에 일어났음을 시사한다. 두 type 간 유전자 교류 정도를 파악하려면 향후 microsatellite와 같은 보다 민감한 마커를 이용한 후속 연구가 필요할 것이다.

• 한국발생생물학회지:발생과생식
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• 제23권4호
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• pp.367-375
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• 2019

Pufferfish (Takifugu spp.) are economically important edible marine fish. Mistakes in pufferfish classification can lead to poisoning; therefore, accurate species identification is critical. In this study, we used the mtDNA cytochrome c oxidase subunit I gene (COI) to design specific primers for six Takifugu species among the 21 domestic or imported pufferfish species legally sold for consumption in Korea. We rapidly and simultaneously identified these pufferfish species using a highly efficient, multiplex polymerase chain reaction (PCR) system with the six species-specific primers. The results showed that species-specific multiplex PCR (multiplex species-specific polymerase chain reaction; MSS-PCR) either specifically amplified PCR products of a unique size or failed. MSS-PCR yielded amplification fragment lengths of 897 bp for Takifugu pardalis, 822 bp for T. porphyreus, 667 bp for T. niphobles, 454 bp for T. poecilonotus, 366 bp for T. rubripes, and 230 bp for T. xanthpterus using the species-specific primers and a control primer (ca. 1,200 bp). We visualized the results using agarose gel electrophoresis to obtain accurate contrasts of the six Takifugu species. MSS-PCR analysis is easily performed and provides identification results within 6 h. This technique is a powerful tool for the discrimination of Takifugu species and will help prevent falsified labeling, protect consumer rights, and reduce the risk of pufferfish poisoning..

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.313-318
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• 2019

In recent years, the taeniasis has been rarely reported in the Republic of Korea (Korea). But in this study, we intend to report 4 taeniasis cases caused by Taenia saginata during a 5-month period (February to June 2018) at a unversity hospital in Gwangju, Korea. Worm samples (proglottids) discharged from all cases were identified by phenotypic and molecular diagnostics. Mitochondrial cytochrome c oxidase subunit I sequences showed 99.4-99.9% identity with T. saginata but, differed by 4% from T. asiatica and by 7% from T. multiceps, respectively. We found that tapeworms in 2 cases (Cases 2 and 3) yielded exactly the same sequences between them, which differed from those in Cases 1 and 4, suggesting intra-species variation in tapeworms. These taeniasis cases by T. saginata infection in this study, which occurred within a limited time period and region, suggest the possibility of a mini-outbreak. This study highlights the need for further epidemiological investigation of potentially overlooked cases of T. saginata infection in Korea.

• 한국어류학회지
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• 제36권2호
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• pp.188-192
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• 2024

동갈돔과에 속하는 Apogon erythrius 1개체(표준체장 44.3 mm)가 2009년 10월 제주도 사계 연안 통발에서 처음으로 채집되었다. 본 종은 등지느러미 앞(predorsal)의 비늘수가 5~6개, 새파수 7~9개이며, 제2등지느러미 극조를 눕혔을 때 제2 등지느러미 기저의 중간을 지나지 않는 점, 비늘의 말단이 진한 분홍색을 띠는 점 등의 특징을 갖는다. 형태 기반의 종 동정을 확인하기 위해 분자 동정을 한 결과, 채집된 개체는 기존에 보고된 Apogon erythrinus의 염기서열과 잘 일치하였다. 몸 크기에 비해 눈이 커서 이 종의 국명을 "큰눈얼게비늘"로 제안한다.

• 한국어류학회지
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• 제36권2호
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• pp.193-198
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• 2024

2023년 9월과 10월에 제주특별자치도 서귀포시 위미항에서 처음으로 동갈돔과에 속하는 1미기록종인 Ostorhinchus fleurieu (54.25 mm, 55.64 mm SL) 표본 2점이 채집되었다. O. fleurieu는 동속의 O. aureus와 매우 유사하지만, 새파수 (O. fleurieu는 19~23 vs O. aureus는 22~27), 꼬리자루 줄무늬의 형태(O. fleurieu는 가장자리가 불분명한 술통형 vs O. aureus는 가장자리가 뚜렷한 모래시계형) 등에서 잘 구분된다. 또한, 동갈돔과 2개체의 COI 염기서열 560 bp는 NCBI에 등록된 O. fleurieu의 염기서열(MT076481)과 100% 일치하였다. O. fleurieu의 새로운 국명으로 "꽃동갈돔"을 제안한다.

• Parasites, Hosts and Diseases
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• 제58권1호
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• pp.93-97
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• 2020

The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to T. hydatigena has large veterinary and economic drawbacks. Like other taeniids, e.g., Echinococcus, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of T. hydatigena, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) mitochondrial genes. The isolates were correctly identified as T. hydatigena with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the nad1 and cox1 genes, respectively. The results suggest the presence of unique T. hydatigena haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of T. hydatigena in Africa, this report represents the first insights into the genetic variation of T. hydatigena in Sudan and constitutes useful data.