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http://dx.doi.org/10.3347/kjp.2020.58.1.93

Molecular Identification of Taenia hydatigena from Sheep in Khartoum, Sudan  

Muku, Rosline James (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Yan, Hong-Bin (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Ohiolei, John Asekhaen (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Saaid, Abubakar Ahmed (Central Veterinary Research Laboratory, Animal Resources Research Corporation)
Ahmed, Sara (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Jia, Wan-Zhong (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Fu, Bao-Quan (State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Publication Information
Parasites, Hosts and Diseases / v.58, no.1, 2020 , pp. 93-97 More about this Journal
Abstract
The cestode Taenia hydatigena uses canids, primarily dogs, as definitive hosts, while the metacestode larval stage cysticercus infects a range of intermediate hosts, including domestic animals such as goats, sheep, and pigs. Cysticercosis due to T. hydatigena has large veterinary and economic drawbacks. Like other taeniids, e.g., Echinococcus, intraspecific variation is found among the members of the genus Taenia. In Africa, few studies are available on the epidemiology and distribution of T. hydatigena, and even fewer studies are available on its genetic variation. In this study, we molecularly identified 11 cysticerci from sheep in Sudan and demonstrated the genetic variation based on the NADH dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) mitochondrial genes. The isolates were correctly identified as T. hydatigena with more than 99% similarity to those in the GenBank database. Low diversity indices and insignificant neutrality indices were observed, with 3 and 2 haplotypes for the nad1 and cox1 genes, respectively. The results suggest the presence of unique T. hydatigena haplotypes in Sudan, as haplotypes with 100% similarity were not found in the GenBank database. With few available studies on the genetic variation of T. hydatigena in Africa, this report represents the first insights into the genetic variation of T. hydatigena in Sudan and constitutes useful data.
Keywords
Taenia hydatigena; genetic variation; cysticercus; Khartoum;
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1 Kusiluka L, Kambarage D, Harrison L, Daborn C, Matthewman R. Causes of morbidity and mortality in goats in Morogoro district, Tanzania: The influence of management. Small Rumin Res 1998; 29: 167-172.   DOI
2 Christodoulopoulos G, Theodoropoulos G, Petrakos G. Epidemiological survey of cestode-larva disease in Greek sheep flocks. Vet Parasitol 2008; 153: 368-373.   DOI
3 Saulawa M, Magaji A, Faleke O, Mohammed A, Kudi A, Musawa A, Sada A, Ugboma A, Akawu B, Sidi S. Prevalence of Cysticercus tenuicollis cysts in sheep slaughtered at Sokoto abattoir, Sokoto state, Nigeria. Sokoto J Vet Sci 2011; 9: 24-27.
4 Braae UC, Kabululu M, Normark ME, Nejsum P, Ngowi HA, Johansen MV. Taenia hydatigena cysticercosis in slaughtered pigs, goats, and sheep in Tanzania. Trop Anim Health Prod 2015; 47: 1523-1530.   DOI
5 Fakae B. The epidemiology of helminthosis in small ruminants under the traditional husbandry system in eastern Nigeria. Vet Res Commun 1990; 14: 381-391.   DOI
6 Sissay MM, Uggla A, Waller PJ. Prevalence and seasonal incidence of larval and adult cestode infections of sheep and goats in eastern Ethiopia. Trop Anim Health Prod 2008; 40: 387-394.   DOI
7 Belem A, Kabore A, Bessin R. Gastrointestinal helminthes of sheep in the central, eastern and northern part of Burkina Faso. Bull Anim Health Prod Afr 2005; 53: 13-23.
8 Attindehou S, Salifou S. Epidemiology of cestodes infections in sheep and goats in Benin. Vet Res 2012; 5: 59-62.
9 El Badawi KS, El Gezuli AY, Eisa AM, Slepnev NK. Incidence of Cysticercus tenuicollis in animals slaughtered for human consumption in Sudan. Sudan J Vet Sci Anim Husb 1978; 19: 87-91.
10 Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 1992; 54: 165-174.   DOI
11 Lavikainen A, Lehtinen MJ, Meri T, Hirvela-Koski V, Meri S. Molecular genetic characterization of the Fennoscandian cervid strain, a new genotypic group (G10) of Echinococcus granulosus. Parasitology 2003; 127: 207-215.   DOI
12 Jia WZ, Yan HB, Guo AJ, Zhu XQ, Wang YC, Shi WG, Chen HT, Zhan F, Zhang SH, Fu BQ, Littlewood DT, Cai XP. Complete mitochondrial genomes of Taenia multiceps, T. hydatigena and T. pisiformis: additional molecular markers for a tapeworm genus of human and animal health significance. BMC Genomics 2010; 11: 447.   DOI
13 Nakao M, Li T, Han X, Ma X, Xiao N, Qiu J, Wang H, Yanagida T, Mamuti W, Wen H, Moro PL, Giraudoux P, Craig PS, Ito A. Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences. Int J Parasitol 2010; 40: 379-385.   DOI
14 Ito A, Yamasaki H, Nakao M, Sako Y, Okamoto M, Sato MO, Nakaya K, Margono SS, Ikejima T, Kassuku AA, Afonso SMS, Ortiz WB, Plancarte A, Zoli A, Geerts S, Craig PS. Multiple genotypes of Taenia solium ramifications for diagnosis, treatment and control. Acta Trop 2003; 87: 95-101.   DOI
15 Zhang Y, Zhao W, Yang D, Tian Y, Zhang W, Liu A. Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived Coenurus cerebralis and Cysticercus tenuicollis isolates in Inner Mongolia, China. Parasite 2018; 25: 1.   DOI
16 Maravilla P, Gonzalez-Guzman R, Zuniga G, Peniche A, Dominguez-Alpizar JL, Reyes-Montes R, Flisser A. Genetic polymorphism in Taenia solium cysticerci recovered from experimental infections in pigs. Infect Genet Evol 2008; 8: 213-216.   DOI
17 Vega R, Pinero D, Ramanankandrasana B, Dumas M, Bouteille B, Fleury A, Sciutto E, Larralde C, Fragoso G. Population genetic structure of Taenia solium from Madagascar and Mexico: implications for clinical profile diversity and immunological technology. Int J Parasitol 2003; 33: 1479-1485.   DOI
18 Omar MA, Elmajdoub LO, Al-Aboody MS, Elsify AM, Elkhtam AO, Hussien AA. Molecular characterization of Cysticercus tenuicollis of slaughtered livestock in Upper Egypt governorates. Asian Pac J Trop Biomed 2016; 6: 706-708.   DOI
19 Ohiolei JA, Luka J, Zhu GQ, Yan HB, Li L, Magaji AA, Alvi MA, Wu YT, Li JQ, Fu BQ, Jia WJ. First molecular description, phylogeny, and genetic variation of Taenia hydatigena from Nigerian sheep and goats based on three mitochondrial genes. Parasit Vectors 2019; 12: 520.   DOI
20 Wu Y, Li L, Zhu G, Li W, Zhang N, Li S, Yao G, Tian W, Fu B, Yin H, Zhu X, Yan H, Jia W. Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau. Parasit Vectors. 2018; 11: 166.   DOI
21 Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 1999; 41: 95-98.
22 Rozas J, Ferrer-Mata A, Sanchez-DelBarrio JC, Guirao-Librado P, Remos-Onsins SE, Sanchez-Gracia A. DnaSP 6: DNA sequence polymorphism analysis of large data set. Mol Biol Evol 2017; 34: 3299-3302.   DOI
23 Liu GH, Lin RQ, Li MW, Liu W, Liu Y, Yuan ZG, Song HQ, Zhao GH, Zhang KX, Zhu XQ. The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Mol Biol Rep 2011; 38: 2249-2256.   DOI
24 Nakao M, Sako Y, Ito A. The Mitochondrial Genome of the Tapeworm Taenia solium: a finding of the abbreviated stop codon U. J Parasitol 2003; 89: 633-635.   DOI
25 Jeon HK, Kim KH, Eom KS. Complete sequence of the mitochondrial genome of Taenia saginata: comparison with T. solium and T. asiatica. Parasitol Int 2007; 56: 243-246.   DOI
26 Jeon HK, Eom KS. Taenia asiatica and Taenia saginata: genetic divergence estimated from their mitochondrial genomes. Exp Parasitol 2006; 113: 58-61.   DOI
27 Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A. Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): Proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria. Int J Parasitol 2013; 43: 427-437.   DOI
28 Le TH, Pearson MS, Blair D, Dai N, Zhang LH, McManus DP. Complete mitochondrial genomes confirm the distinctiveness of the horse-dog and sheep-dog strains of Echinococcus granulosus. Parasitology 2002; 124: 97-112.   DOI
29 Boufana B, Scala A, Lahmar S, Pointing S, Craig PS, Dessi G, Zidda A, Pipia AP, Varcasia A. A preliminary investigation into the genetic variation and population structure of Taenia hydatigena from Sardinia, Italy. Vet Parasitol 2015; 214: 67-74.   DOI