• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.138-144
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• 2008

The production of the entomopathogenic and phytotoxic cyclic depsipeptide beauvericin (BEA) was studied in submerged cultures of Fusarium oxysporum KFCC 11363P isolated in Korea. The influences of various factors on mycelia growth and BEA production were examined in both complete and chemically defined culture media. The mycelia growth and BEA production were highest in Fusarium defined medium. The optimal carbon and nitrogen sources for maximizing BEA production were glucose and $NaNO_3$, respectively. The carbon/nitrogen ratio for maximal production of BEA was investigated using response surface methodology (RSM). Equations derived by differentiation of the RSM model revealed that the production of BEA was maximal when using 108 mM glucose and 25 mM $NaNO_3$.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.8
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• pp.1450-1454
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• 2007

This paper presents a microfabricated Clark-type sensor which exactly can measure dissolved oxygen in the cell containing solution. We designed, fabricated, and characterized a microfabircated Clark-type oxygen sensor for measuring dissolved oxygen. The microfabricated oxygen sensor consists of 3-electrodes on a glass substrate, a FEP (Fluorinated ethylene propylene) oxygen-permeable membrane, and PDMS (Polydimethylsiloxane) reservoir for storing sample solution. Thin-film Ag/AgCl was employed as a reference electrode and its durability was verified by obtaining a stable open circuit potential for 2 hours against a commercial Ag/AgCl electrode and a stable cyclic voltammetry curve. Selectivity, response time, and linearity of the fabricated oxygen sensor were also verified well by cyclic voltammetry and amperometry depending. The fabricated oxygen sensor showed a 90% response time of 40sec and an excellent linearity with a correlation coefficient of 0.994.

• Applied Chemistry for Engineering
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• v.27 no.2
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• pp.185-189
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• 2016

Among many kinds of bioaffinity sensors, the avidin-biotin system has been widely used in a variety of biological applications due to the specific and high affinity interaction of the system. In this work, gold nanorods with high surface area were explored as electrodes in order to amplify the signal response from the avidin-biotin interaction which can be further utilized for avidin-biotin biosensors. Electrochemical performance of electrodes modified with nanorods and functionalized with avidin in response to interactions with biotin at various concentrations using $[Fe(CN)_6]^{3-/4-}$ couple as the redox probe were investigated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). A very low biotin concentration of less than 1 ng/mL could be detected using the electrodes modified with nanorods.

• Earthquakes and Structures
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• v.9 no.2
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• pp.281-303
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• 2015

The effects of infill panels on the response of r.c. frames subjected to seismic action are widely recognized. Numerous experimental investigations were effected and several analytical models were developed on this subject. This work, which is part of a larger project dealing with specific materials and structures commonly used in Italy, discusses experimental tests on masonry and samples of bare and infilled portals. The experimental activity includes tests on elemental materials, and 12 wall samples. Finally, three one-bay one-story reinforced concrete frames, designed according to the outdated Italian technical code D.M. 1996 without seismic details, were tested (bare and infilled) under constant vertical and cyclic lateral load. The first cracks observed on the framed walls occurred at a drift of about 0.3%, reaching its maximum capacity at a drift of 0.5% while retaining its capacity up to a drift of 0.6%. Infill contributed to both the stiffness and strength of the bare reinforced concrete frame at small drifts thus improving overall system behavior. In addition to the experimental activities, previously mentioned, the recalibration of a model proposed by Comberscue (1996) was evaluated. The accuracy of an OpenSees non linear fiber based model of the prototype tested, including a strut element was verified through a comparison with the final experimental results. This work has been partially supported by research grant DPC-ReLUIS 2014.

• Structural Engineering and Mechanics
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• v.29 no.3
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• pp.259-278
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• 2008

This paper presents a nonlinear finite element procedure accounting for the effects of geometric as well as material nonlinearities for reinforced concrete bridge piers supported by laminated rubber bearings. Reinforced concrete bridge piers supported by laminated rubber bearings and carrying a cyclic load were analyzed by using a special purpose, nonlinear finite element program, RCAHEST. For reinforced concrete, the proposed robust nonlinear material model captures the salient response characteristics of the bridge piers under cyclic loading conditions and addresses with the influence of geometric nonlinearity on post-peak response of the bridge piers by transformations between local and global systems. Seismic isolator element to predict the behaviors of laminated rubber bearings is also developed. The seismic performance of reinforced concrete bridge piers supported by laminated rubber bearings is assessed analytically. The results show good correlation between the experimental findings and numerical predictions, and demonstrate the reliability and robustness of the proposed analytical model. Additionally, the studies and discussions presented in this investigation provide an insight into the key behavioral aspects of reinforced concrete bridge piers supported by laminated rubber bearings.

• Earthquakes and Structures
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• v.19 no.6
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• pp.485-498
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• 2020

Non-ductile detailing of Reinforced Concrete (RC) frames may lead to structural failure when the structure is subjected to earthquake response. These designs are generally encountered in older RC frames constructed prior to the introduction of the ductility aspect. The failure observed in the beam-column joints (BCJs) and accompanied by excessive column damage. This work examines the seismic performance and failure mode of non-ductile designed RC columns and exterior BCJs. The design was based on the actual building in Tainan City, Taiwan, that collapsed due to the 2016 Meinong earthquake. Hence, an experimental investigation using cyclic testing was performed on two columns and two BCJ specimens scaled down to 50%. The experiment resulted in a poor response in both specimens. Excessive cracks and their propagation due to the incursion of the lateral loads could be observed close to the top and bottom of the specimens. Joint shear failure appeared in the joints. The ductility of the member was below the desired value of 4. This is the minimum number required to survive an earthquake with a similar magnitude to that of El Centro. The evidence provides an understanding of the seismic failure of poorly detailed RC frame structures.

• Coupled systems mechanics
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• v.12 no.5
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• pp.409-418
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• 2023

A coupled algorithm is proposed which first considers the creation of porous structure of the material and then the simulations of response of mechanical components with porous structure to a variable load history. The simulations are carried out by the Prandtl operator approach in the finite element method (FEM) which enables structural simulations of mechanical components subjected to variable thermomechanical loads. Temperature-dependent material properties and multilinear kinematic hardening of the material can be taken into account by this approach. Several simulations are then performed for a tensile-compressive specimen made of a generic porous structure and mechanical properties of Aluminium alloy AlSi9Cu3. Variable mechanical load history has been applied to the specimens under constant temperature conditions. Comparison of the simulation results shows a considerable elastoplastic stress-strain response in the vicinity of pores whilst the surface of the gauge-length of the specimen remains in the elastic region of the material. Moreover, the distribution of the pore sizes seems more influential to the stress-strain field during the loading than their radial position in the gauge-length.

• Computational Structural Engineering
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• v.5 no.1
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• pp.119-132
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• 1992

The objective of this study is to perform extensive parametric studies of the lateral-torsional buckling of short 1-beams under repeated loadings, and to gain a further insight into the lateral-torsional beam buckling problem. A one-dimensional geometrically (fully) nonlinear beam model is used, which includes superposed infinitesimal transverse warping deformation in addition to finite torsional warping deformation. A multiaxial cyclic plasticity model is also implemented to better represent cyclic metal plasticity in conjunction with a consistent return mapping algorithm. The general response for the lateral-torsional buckling of short I-beams under repeated loadings is examined through several parametric studies around the standard case : the material yield strength, the yield plateau, the strain hardening, the kinematic hardening, the residual stresses, the load eccentricity with respect to the shear center, the height of the load with respect to the cross-section of the beam, the location of the load along the length of the beam, the dimensions of the cross-section of the beam and the fixity of the supported end remote from the load.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.1
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• pp.176-182
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• 2008

Scolopendra was known to cure erectile dysfunction. This study was aimed to investigate the effects of Scolopendra on the nitric oxide synthase activity, nitrite level, cyclic-GMP and erectile responses in rat's corpus cavernosum penis. The crushed Scolopendra was extracted 3 times, each time with 3 volumes of methyl alcohol at $60^{\circ}C$ for 24 h. The extract was filtered and evaporated under a reduced pressure using a rotary evaporator to yield 14.2 g. Scolopendra extract was oral-administered 50 mg per 1 kg of body weight for 30 days. First, samples were treated with Scolopendra, and then ethanol-treated rats and L-N-Nitroarginine methyl ester (L-NAME) treated rats were fed with the samples. The level of urethral nitrite and nitric oxide synthase activity in the ethanol-Scolopendra double administered rats were as high as in the normal group, while the one in the ethanol-treated group was decreased. The level of urethral cyclic-GMP and guanylate cyclase actiyity in the ethanol-Scolopendra double administered rats were as high as in the normal group, while the one in the ethanol-treated group was decreased. The level of urethral phosphodiesterase activity in the ethanol-Scolopendra double administered rats was as low as in the normal group, while the one in the ethanol-treated group was increased. The erectile response to a cavernous nerve stimulation in L-NAME $(10^{-4})-treated$ rats was restored by the Scolopendra to the similar level seen in the normal group. The electile response to cavernous nerve stimulation in the ethanol-Scolopendra double administered rats were increased as high as in the normal group while the one in the ethanol-treated group was decreased. Scolopendra was effective in restoring the ethanol-induced or L-NAME-induced erectile dysfunction in rats.

• Journal of the Korean Geotechnical Society
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• v.22 no.10
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• pp.173-181
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• 2006

A Two Mobilized-Plane Model is proposed for monotonic and cyclic soil response including liquefaction. This model is based on two mobilized planes: a plane of maximum shear stress, which rotates, and a horizontal plane which is spatially fixed. By controlling two mobilized planes, the model can simulate the principal stress rotation effect associated with simple shear from different $K_0$ states. The proposed model gives a similar skeleton behaviour for soils having the same mean stress, regardless of $K_0$ conditions as observed in laboratory tests. The soil skeleton behaviour observed in cyclic drained simple shear tests, including compaction during unloading and dilation at large strain is captured in the model. Undrained monotonic and cyclic response is predicted by imposing the volumetric constraint of the water on the drained or skeleton behaviour. This constitutive model is incorporated into the dynamic coupled stress-flow finite difference program of FLAC (Fast Lagrangian Analysis of Continua). The model was first calibrated with drained simple shear tests on Fraser River sand, and verified by comparing predicted and measured undrained behaviour of Fraser River sand using the same input parameters.