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Biochemical Characterization of a Putative Calcium Influx Factor as a Diffusible Messenger in Jurkat Cells, Xenopis Oocytes, and Yeast

  • Kim, Hak-Yong
    • Animal cells and systems
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    • v.7 no.1
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    • pp.75-79
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    • 2003
  • Highly purified high performance thin layer chromatography (HPTLC) fractions containing a putative calcium influx factor (CIF) were prepared from the Jurkat cells and Xenopus oocytes in which $Ca^{2+}$ stores were depleted by thapsigargin treatment and from the yeast in which intracellular $Ca^{2+}$ stores were also depleted by genetic means. Microinjection of the fractions has been shown to elicit $Ca^{2+}$ dependent currents in Xenopus oocytes. The nature of the membrane currents evoked by the putative CIF appeared to be carried by chloride ions since the current was blocked by the selective chloride channel blocker 1 mM niflumic acid and its reversal potential was about -24 mV. Injection of the calcium chelator 1,2-bis(2-aminophenoxy)ethane-N, N, N',N'-tetraacetic acid (BAPTA) eradicated the current activities, suggesting the current responses are entirely $Ca^2$-dependent. Moreover, the currents were sensitive to the removal of extracellular calcium, indicating the dependence on calcium entry through the plasma membrane calcium entry channels. CIF activities were insensitive to protease, heat, and acid treatments and to Dische-reaction whereas the activities were sensitive to nucleotide pyrophosphatase and hydrazynolysis. The fraction might have a sugar because it was sensitive to Molisch test and Seliwaniff's resorcinol reaction. From the above results, CIF as a small and stable molecule seems to have pyrimidine, pyrophosphate, and a sugar moiety.oiety.

Integrated Microdisk Gold Electrode Modified with Metal-porphyrin and Metal-phthalocyanines for Nitric Oxide Determination in Biological Media

  • Kim, Il-Kwang;Bae, Hyun-Ok;Oh, Gi-Soo;Chung, Hun-Taeg;Kim, Young-Jin;Chun, Hyun-Ja
    • Bulletin of the Korean Chemical Society
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    • v.24 no.11
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    • pp.1579-1584
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    • 2003
  • An integrated gold microdisk electrode was constructed and modified with metal-porphyrin or metal-phthalocyanines for NO determination in biological media. Microanalysis of NO using square wave anodic stripping voltammetry in $1\;{\times}\;10^{-2}$ M $HClO_4$ was optimal when the accumulation potential was 0.1 V, frequency 100 Hz, and the scan rate was 200 mV/s. When the electrode was modified with metal-porphyrin or metal-phthalocyanines, the anodic peak currents of NO increased due to the catalytic oxidation of NO. In case of Fe(II)-phthalocyanine modified electrode, the peak currents remarkably increased and the sensitivity was high. The calibration curve had good linearity in the range from $3.6\;{\times}\;10^{-5}$ M to $7.2\;{\times}\;10^{-7}$ M, and the detection limit was $5.7\;{\times}\;10^{-7}$ M. For the structural stability and increased sensitivity, Fe(II)-phthalocyanine modified gold microdisk electrode coated with Nafion was applied to determination of NO released from cultured macrophase.

The Stress Dependence of Trap Density in Silicon Oxide

  • Kang, C. S.
    • Journal of the Institute of Electronics Engineers of Korea TE
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    • v.37 no.2
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    • pp.17-24
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    • 2000
  • In this paper, the stress and transient currents associated with the on and off time of applied voltage were used to measure the density and distribution of high voltage stress induced traps in thin silicon oxide films. The transient currents were due to the discharging of traps generated by high stress voltage in the silicon oxides. The trap distributions were relatively uniform new both cathode and anode interface. The trap densities were dependent on the stress polarity. The stress generated trap distributions were relatively uniform the order of 1011~1021[states/eV/cm2] after a stress voltage. It appear that the stress and transient current that flowed when the stress voltage were applied to the oxide was caused by carriers tunneling through the silicon oxide by the high voltage stress generated traps.

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A New Low Cost Hybrid Power Filter for Thyristor-Controlled Rectifier Load (싸이리스터 정류부하를 위한 새로운 저원가 복합형 전력필터)

  • 한성룡;김수근;석원엽;조정구;송의호;전희종
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.53 no.1
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    • pp.24-29
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    • 2004
  • A new low cost hybrid active filter for thyristor-controlled rectifier load is presented to overcome the high cost problem of the active or the other hybrid active filters. The proposed hybrid active filter which consists of tuned (5th and 7th harmonics) LC passive filters, power factor improvement(PFI) capacitor bank, and active filter compensates power factor as well as harmonic currents. Since most of harmonic currents are filtered by the passive filter and most of reactive power is compensated by the PFI capacitor bank, the power rating of active filter can be minimized, resulting in cost minimization of the proposed hybrid active filter. A 300kVA hybrid active filter system is implemented and tested using 1MVA thyristor rectifier load to verify the operation and performance.

Analysis of Power System Dynamic Security Due to Operation of 345kv CLR (345kv한류리액터 운전에 따른 전력계통 동적안전도 해석)

  • Lee Baek Seok;Kang Dae Eon;Lee Kang Wan
    • Proceedings of the KIEE Conference
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    • summer
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    • pp.24-26
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    • 2004
  • The growing demand for electricity has result in a corresponding increase in power generated and transmitted. As the increase in installed capacity of power system, the fault currents of 345kv system will be increased beyond the interrupting duty of the circuit breakers. The current limiting reactors are used to limit the fault current. The paper presents the result of the transient stability and voltage stability due to install 345kV current limiting reactor to suppress the fault currents.

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A Study on the Residual Current in the Cheju Strait (제주해협의 해수유동특성에 관한 연구)

  • KIM Sang Hyun;RHO Hong Kil
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.5
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    • pp.759-770
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    • 1997
  • The general flow patterns in the Cheju Strait have been investicated by analyzing the current observations measured in $1986\~1989$ by current meter mooring in 3 north-south sections in the Cheju Strait and at 4 observation points around Cheju Harbour, and measured in $1981\~1987$ by drogue tracking. 1. In the Cheju Strait, there are eastward or northeastward residual currents, which implies that sea waters flow into through the whole western section and flow out through the whole eastern section in the Cheju Strait. The velocity of residual currents are $5.2\~30\;cm/sec$ in 10 m layer and $1.3\~24cm/sec$ in mid-bottom layer. Generally, the flow is strong along the deepest through and the northern part, and weak in the shallow areas near Chuja Islands and Bogil Island. 2. In the western entrance of the Cheju Strait, the observed mean residual velocity is 6.93 cm/sec and the volume transport is 0.384 Sv. There are a big discrepancy between the observed residual currents and the geostrophic currents. 3. Near the frontal areas northwest to Chuja Islands, warm and saline offshore waters, flow northward about 5 miles into the southern coastal areas of the Korean Peninsula in flood, and flow back rather eastward or southeastward than southward in ebb. So, warm and saline waters flow along coastal areas, being mixed with coastal waters. As a result, the northwestern area of Chuja Islands plays a role of the entrance of influx of warm and saline offshore water to the southwestern coastal areas of the Korean Peninsula. It should be stressed that this flow pattern is not due to the residual flows, but to the temporal (tidal) flows.

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Impact of the coastal structures on the water circulation near Gusipo coast, Yellow Sea, Korea (서해 구시포 해안에서 해수유동에 미치는 구조물의 영향)

  • Kim, Cha-Kyum;Park, Il Heum
    • Journal of Korea Water Resources Association
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    • v.55 no.11
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    • pp.865-875
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    • 2022
  • Field measurements and numerical simulations using EFDC model were performed to quantify the changes of water circulation near Gusipo coast located in the Yellow Sea of Korea to estimate the impact of the construction of the coastal structures (jetty, groin, Gusipo port and bridge). The model predicted tide and tidal currents agreed reasonably well with the measurements. The maximum currents during spring tide near the Gusipo Beach (GB) have the range of 20~40 cm/sec whereas those off the GB range from 60 to 80 cm/sec. The typical patterns of tidal current show parallel with the local isobath. Tidal currents flow northeastward during the flood tide whereas the currents during the ebb tide flow southwestward. The current speeds at shielded waters after the construction of coastal structures strongly decreased as compared with those before the construction. The tidal volume due to the construction of coastal structures was estimated using the depth averaged velocity for 24 hours of spring tide. Tidal volume after construction of coastal structures was compared with initial state (before construction). Tidal volume at present state (after construction of jetty, groin, Gusipo port and bridge) decreased by 28.4% as compared with that of the initial state. The volume after construction of jetty and groin decreased by 21.3%, and the volume after construction of Gusipo port and bridge decreased by 9.8%.

Ginsenosides Inhibit N-, p-, arid Q-types but not L-type of $Ca^{2+}$ Channel in Bovine Chromaffin cells

  • Seok Chol;Jung, Se-Yeon;Kim, Hyun-Oh;Kim, Hack-Seang;Hyewhon Rhim;Kim, Seok-Chang;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • v.24 no.1
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    • pp.18-22
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    • 2000
  • In previous reports we have shown that ginsenosides inhibit high threshold voltage-dependent $Ca^{2+}$ channels in neuronal cells. However, these studies did not show whether ginsenosides-induced inhibition of $Ca^{2+}$ currents discriminates among the various $Ca^{2+}$ channel subtypes, although it is known that there are at least five different $Ca^{2+}$ channel subtypes in neuronal cells. In this study we investigated the effect of ginsenosides on high threshold voltage-dependent $Ca^{2+}$ channel subtypes using their selective $Ca^{2+}$ channel blockers nimodipine (L-type), $\omega$-conotoxin GVIA (N-type), or $\omega$-agatoxin IVA (P-type) in bovine chromaffin cells. We could observe that ginsenosides inhibited high threshold voltage-dependent $Ca^{2+}$ currents in a dose-dependent manner. The $IC_{50}$/ was about 120 $\mu$g/ml. Nimodipine had no effect on ginsenosides response. However, the effect of ginsenosides on $Ca^{2+}$ currents was reduced by $\omega$-conotoxin GVIA, $\omega$-agatoxin IVA, and mixture of nimodipine, $\omega$-contoxin GVIA, and $\omega$-agatoxin IVA. These data suggest that ginsenosides are negatively coupled to three types of calcium channels in bovine chromaffin cell, including an $\omega$-conotoxin GVIA-sensitive (N-type) channel, an $\omega$-agatoxin IVA-sensitive (P-type) channel and nimodipine/$\omega$-conotoxin GVIA/$\omega$-agatoxin IVA-resistant (presumptive Q-type) channel.Q-type) channel.

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Finite Element Analysis of the Transient Characteristics of a Superconducting A.C. Generator (유한요소법에 의한 초전도교류 발전기의 과도 특성 해석)

  • 한성진;배동진
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.40 no.1
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    • pp.24-30
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    • 1991
  • This paper deals with the analysis of the transient characteristics of a superconducting a.c. generator(SCG) using Finite Element Method. Since the magnetic field induced by the field current and the armature currents are not sinusoidally distributed in a generator, the conventional equivalent circuit method, in general, uses the fundamental component only and is done in frequency domain. But the finite element analysis makes it possible to analyze the transient magnetic field distribution and the electrical characteristics of the double shields of SCG in time domain.

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A new design of the SCR type speed controller for the battery powered electric vehicles (SCR을 이용한 전기자동차의 속도제어장치의 설계와 시작)

  • 황희융
    • 전기의세계
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    • v.24 no.6
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    • pp.85-91
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    • 1975
  • A new design method for the SCR-Chopper type speed controller of the battery-powered vehicles was developed. The conventional speed-controllers were either inductance type or contact type using resistance. In the new method, a photocoupler was used in sensing the control currents and a noncontacting type photo-conductive device (CDS) was used in mading acceleration. Experiments using these new devices showed encouraging results.

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