• Title/Summary/Keyword: culture-independent

Search Result 827, Processing Time 0.029 seconds

A Study on the Effect of Cultural Marketing on Fashion Brand Image Management (문화마케팅을 통한 패션업체의 브랜드 이미지 관리에 관한 연구 -패션브랜드와 문화예술 이미지 부합도를 중심으로-)

  • Hwang, Sun-Jin;Lee, Yun-Kyung
    • Journal of the Korean Society of Clothing and Textiles
    • /
    • v.32 no.2
    • /
    • pp.223-234
    • /
    • 2008
  • This Study is designed to identify the appropriate fields of culture and art supporting activities in fashion business and to propose an effective culture and art strategy on establishment of positive fashion brand attitude. Subjects of this study were 242 young male and female consumers interested in fashion and culture marketing activities. Data were analyzed by using SPSS 12.0 with Independent-Sample T-Test, ANOVA, Duncan test. Also, we suggested the FBFit(Fashion Brand-Mecenat Fit) model by using Prefmap3. The results are as follows: First, it was revealed that culture art marketing activities such as a film, a popular music, a musical of the art and culture fields were important for maintaining the positive fashion brand image. Second, the consumer with recognition of culture and art supporting activities had a positive attitude on culture and art marketing of fashion brand. Third, FBFit model was revealed that there was a significant difference an appropriate culture and art fields for each fashion brand image. Therefore, each fashion brand has to select appropriate fields of culture and art marketing activities by considering their fashion brand image with a long term perspective.

Diversity of Marine Microbes by PCR-DGGE (PCR-DGGE를 이용한 해양미생물의 다양성 조사)

  • Kim, Yeong-Jin;Cho, Hyo-Jin;Yu, Sun-Nyoung;Kim, Kwang-Youn;Kim, Hyeung-Rak;Ahn, Soon-Cheol
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.40 no.6
    • /
    • pp.356-361
    • /
    • 2007
  • Recently, the development of various culture-independent identification techniques for environmental microbes has greatly enhanced our knowledge of microbial diversity. In particular, denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, generated using the polymerase chain reaction (PCR) is frequently used to examine the diversity of environmental bacterial populations. This method consists of direct extraction of the environmental DNA, amplification of the 200-600 bp 16S rDNA fragments with universal primers, and separation of the fragments according to their melting point on a denaturing gradient gel. In this study, we investigated the seaside microbial community in coastal areas of Busan, Korea, using culture-independent techniques. First, marine genomic DNA was extracted from seawater samples collected at Songjeong, Gwangahn, and Songdo Beaches. Then, PCR was used to amplify the bacterial 16S rDNA using universal primers, and DGGE was used to separate the amplified 500 bp 16S rDNA fragments. Finally, the tested 16S rDNA genes were further analyzed by sequencing. Based on these experiments, we found that DGGE analysis clearly showed variation among the regional groups. It can be used to monitor rapid changes in the bacterial diversity of various environments. In addition, the sequence analysis indicated the existence of many unculturable bacteria, in addition to Arcobacter, Pseudoaltermonas, and Vibrio species.

Job Satisfaction of Employees in F&B Department: Domestic Independent Deluxe Hotel vs. International Chain Deluxe Hotel (국내 비체인 특급 호텔과 외국계 체인 특급 호텔 조리.식음료 종사원의 직무만족도 비교 분석)

  • Lee, So-Jung;Myung, Me-Sun;Yang, Il-Sun;Lee, Hae-Young
    • Journal of the Korean Society of Food Culture
    • /
    • v.21 no.1
    • /
    • pp.42-50
    • /
    • 2006
  • The purposes of this study were to measure the job satisfaction levels of F&B(Food & Beverage) personnel in hotel by using JDI(Job Descriptive Index), to investigate relationships between demographic variables or job type and job satisfaction, to determine the relative importance of the five facets and to identify the effects of job satisfaction on turnover intention by hotel. Questionnaire was developed and distributed to 660 employees who are currently working in food & beverage department of hotel A (Domestic independent deluxe hotel) and hotel B(international chain deluxe hotel). A total of 498 questionnaires were usable; resulting in 75.5% response rate. The survey was conducted between March 16 to 27, 2000. As a result of analysis on job satisfaction, total JDI score was 136.57 out of full mark 210. Personnel of Hotel A was more satisfied significantly with work itself (p<.01), supervision (p<.001), co-worker (p<.01), total JDI (p<.001) than those of hotel B. Total JDI and work itself score were higher on employees of front of the house than those of back of the house in both of hotels. Payroll was the most important facet on job satisfaction. Finally, work itself (p<.001) and payroll (p<.05) had a significant effect on turnover intention in hotel A and work itself (p<.001), supervision (p<.05) and promotion (p<.05) did in hotel B. The findings indicated the necessity of human resource management apt to the vision of enterprise and the organizational culture.

Analysis of Bacterial Diversity in Fermented Skate Using Culture-dependent and Culture-independent Approaches (배양 의존적 및 배양 비의존적 방법에 의한 홍어회 서식 미생물의 다양성 분석)

  • Lee, Eun-Jung;Kim, Tae-Hyung;Kim, Ha-Kun;Lee, Jung-Kee;Kwak, Hahn-Shik;Lee, Jong-Soo
    • Microbiology and Biotechnology Letters
    • /
    • v.38 no.3
    • /
    • pp.322-328
    • /
    • 2010
  • Fermented skate is a traditional Korean food popular in Southwestern area of Korea. It has a characteristic flavor and alkaline pH. In this study we tried to determine the microbial flora in fermented skate using two different approaches. In culture-independent method, we amplified V2 region of 16S rRNA gene by PCR and cloned them into pUC18 plasmid to construct 16S rDNA fragment library. BLAST searches for the sequences obtained from this library revealed that uncultured bacterium clone 054E11.b was the most dominant flora in this fermented fish. In culture-dependent method, we diluted suspension of skate and spreaded on MRS, PCA, and MacConkey plates. We identified colonies grown on those plates by using PCR amplification of V2 region of 16S rRNA and DNA sequencing. BLAST searches of those DNA sequences resulted in totally different species with the observations from the 16S rDNA library analysis. Discrepancies of results obtained from both approaches suggest that the agar plates used in culture-dependent method may be different from the real condition of fermented skate. Therefore, results from culture-independent approach using 16S rDNA fragment library analysis may reflect real microbial flora in fermented skate.

Evaluation of Riverine Microbial Diversity using the Culture-Independent Genetic Fingerprinting Technique (T-RFLP) (유전자지문분석법(T-RFLP)을 이용한 하천 미생물의 다양성 평가)

  • Jeong, Ju-Yong;Lee, Kyong-Hee
    • Journal of Korean Society on Water Environment
    • /
    • v.24 no.2
    • /
    • pp.195-200
    • /
    • 2008
  • To analyze the riverine microbial community structure, genetic fingerprints and ecological indexes such as species abundances, diversity, evenness, dominance of targeted rivers in Gyeonggi Province were acquired and evaluated using terminal restriction fragment length polymorphism (T-RFLP) technique. Genetic fingerprinting technique such as T-RFLP, which is able to show the microbial community clearly unlike traditional culture-dependent techniques, was thought to be useful to analyse the riverine microbial ecosystem under various factors. Riverine ecosystem evaluation using visible organisms would give biased results with time, targeted organism and researcher. But, T-RFLP, which can exclude the subjected biases such as culture condition and identification, would be an option to understand natural ecosystem by including the microorganisms that defy culture but perform important functions.

A Study on Anti-Design and Italian Radical Fashion (안티디자인과 이탈리아 급진주의 패션에 관한 연구)

  • 이현미
    • The Research Journal of the Costume Culture
    • /
    • v.9 no.1
    • /
    • pp.154-168
    • /
    • 2001
  • This study is intended to understand an implication and significant of anti-design and Italian radical fashion. Anti-design and radicalism is the main stream or origin and essence of post-modernism which hs been swaying our society at large through modern culture, economy, art, fashion etc, from the latter part of 1960s. In this paper, the author classified the italian radical fashion with the Archizoom, the Alchymia, the memphis fashion. In post-modernism fashion, radical fashion design of the Archizoom, Alchymia and Memphis group is in part a menifesto and in part a noncanonical history of the most progressive and heretical experiments of the world of fashion. Anti-designers of Italy call for a theory an practive in which the old methods and instruments and the old commendments of modernism are banished. They looked on fashion as material culture, a creative field with its own independent foundation and endowed with its own strong artistic intuition. IF radical design is dead, the energy it has stirred up is still alive nd kicking. One of the most progressive and well-informed culture milieus in Milian, seved as a point of reference for the vanguard of fashion.

  • PDF

A Causality between Cultural Satisfaction and Social Trust in Cities (도시인의 문화환경 만족과 사회적 신뢰의 인과성)

  • Kim, Dong-Yoon
    • Journal of The Korean Digital Architecture Interior Association
    • /
    • v.12 no.4
    • /
    • pp.49-57
    • /
    • 2012
  • With regard to the culture in cities this study aims to essential understanding and systematic approach to the culture. The "2011 Seoul Survey"report has been used to find out causality among the related variables. In the first place 'satisfaction of cultural condition' was operationally selected as a dependent variable for regression. For the purpose of controlling confounding factors for ceteris paribus effect correlation analysis was done between the dependent variable and all other variables respectively, which resulted in two groups of variables: group (1) - 6 variables of very significant correlations(p-value<0.01) and (2) - the other 6 variables of significant correlations(p-value<0.05). Then hierarchical regression was adopted to these 2 groups to analyse $R^2$ increment, statistical significance of independent variables, and multicollinearity(VIF; variance inflation factor). At last a regression model specified by group (1) as independent variables(they are 'social trust', 'satisfaction of walking condition', 'happiness index', 'preparation against old age', 'satisfaction of traffic condition' and 'hours for internet') shows that only 'social trust' variable has statistically significant and substantially strong effect on 'satisfaction of cultural condition.' This finding should be accepted on the following understanding; (1) urban culture has a collective attribute formed between people and society, (2) culture is somewhat telling and hearing stories and the confidence between tellers and hearers is essential in the mutual response and (3) stimulus is received by relationship in company with sense, emotion, thinking and action. In spite of restrictive external validity this finding can be used as a direction for promoting culture and a basis for related policy choice in cities.

Penicillium Diversity from Intertidal Zone in Korea

  • Park, Myung Soo;Lee, Seobihn;Oh, Seung-Yoon;Lim, Young Woon
    • 한국균학회소식:학술대회논문집
    • /
    • 2016.05a
    • /
    • pp.11-11
    • /
    • 2016
  • Penicillium species are commonly isolated from various outdoor and indoor environments, including marine environments such as sponges, algae and sand. Penicillium is especially important because numerous bioactive compounds have been isolated. Penicillium was the most common species in intertidal zone in Korea, however the diversity and ecological roles of Penicillium in intertidal zone are not clarified. We explored diversity and ecological roles of marine-derived Penicillium from tidal flat and sea sand in Korea. The diversity of marine-derived Penicillium from Korea was investigated using both culture-dependent and culture-independent approach by ${\beta}$-tubulin sequence. In addition, we evaluated optimal temperature, halo-tolerance, and enzyme activity of Penicillium strains, such as extracellular alginase, endoglucanase, ${\beta}$-glucosidase, and protease. For culture-dependent approach, a total of 182 strains of 62 Penicillium species were isolated, with 53 species being identified. The most common species was Penicillium oxalicum, followed by P. crustosum, P. brasilianum, P. koreense, and P. griseofulvum. Species richness and composition were not significantly different by season, substrates, and seaside. For culture-independent approach using Illumina sequencing, 73 OTUSs were detected. The most frequently observed species was P. antarcticum, followed by P. koreense, P. crustosum, and P. brevicompactum. Diversity of Penicillium was higher during winter season than during summer season and in western sea than in southern sea, respectively. Community structure was significantly different by season and sea side. 52 species were detected by both methods. Unique species were isolated from each of methods - 10 from culture methods and 21 from Illumina sequencing. Furthermore, salinity adaption of the Penicillium varied depending on species. Many Penicillium species showed endoglucanase, ${\beta}$-glucosidase, and protease activity. Some species including P. paneum and P. javanicum degraded the polycyclic aromatic hydrocarbons. Thus, our results demonstrate that intertidal zone in Korea harbors diverse Penicillium community and marine-derived Penicillium play important ecological roles as decomposers of organic material in marine environments.

  • PDF

Design as a Factor in the Formation of Worldview Paradigms in Culture

  • Lopukhova, Natalia;Dementovich, Tetyana;Misheniuk, Anastasiia;Peresunko, Olena;Ternopolska, Yana
    • International Journal of Computer Science & Network Security
    • /
    • v.21 no.12spc
    • /
    • pp.437-442
    • /
    • 2021
  • This article highlights the importance of design in the formation of worldview paradigms in global culture. The research aims to investigate the design development as a part of the culture and its significance in the social development of society and worldview standards. The article uses general scientific methods of cognition, showing the transformation of design in the social and cultural sphere based on scientific and business literature. The research results show the chronology of design development as a factor determining the development of society and its worldview. In particular, the epoch of modernism, classical functionalism, postmodernism, post-industrialism, and digital design show their own particularities of design development. Furthermore, the article reveals the peculiarities of design development and its requirements depending on the development of society, progress, and civilization in general. The question of current and future trends in the development of design as a factor of worldview paradigms in the culture is based on digitalization, globalization, and the formation of design as an independent entity that determines man's place in the world and society.

The Effects of Online Home Learning in Connection with Extracurricular Activities for Lifelong Education for the Disabled at University on Cafeterias Cooking Assistance Skills of Job Search Persons with Developmental Disabilities

  • Kim, Young-Jun;Park, Jae-Kook;Kwon, Ryang-Hee
    • International Journal of Advanced Culture Technology
    • /
    • v.9 no.3
    • /
    • pp.188-201
    • /
    • 2021
  • The purpose of this study is to analyze the effects of online home learning in connection with extracurricular activities for lifelong education for the disabled in university on the cooking aids skills of cafeterias for the job search persons with developmental disabilities. Three people with job search developmental disabilities who have been in a state of unemployment for three years after graduating from a special high school course participated in the experiment. In order to verify the meaningful functional relationship between independent variables and dependent variables, multiple probe design across subjects, one of the main techniques of a single object study, was used. The experimental conditions according to the research design consisted of the steps of baseline, intervention, maintenance, and generalization. The dependent variable of this study is the restaurant cooking aid skills in the cafeteria, and three subskills such as side dish arrangement, sink arrangement, and dish washing were combined by task analysis. And the independent variable of this study was composed of procedures and methods to teach the environment, tools and materials related to the performance of dependent variables to the developmental disabled people at home by using real-time image technique through zoom service, and the contents of the performance by stages of task analysis. In addition, independent variables were applied to the subjects in the course of the extracurricular activities with the theme and contents of lifelong education for the disabled at university. Students who completed the above extracurricular activities practiced the intervention scene of the researcher through the screen sharing of zoom service. As a result, the subjects with developmental disabilities effectively acquired and maintained the positive response performance of dependent variables through independent variables. The subjects also showed high positive responses to generalization tests conducted in kitchens in cafeterias located elsewhere in the same university.