• Title/Summary/Keyword: culture time

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Outdoor Table Design Study of Applying the Structure of Soban (소반의 구조를 적용한 Outdoor Table 디자인 연구)

  • Go, Young-Lok;Kim, Chung Ho
    • Journal of the Korea Furniture Society
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    • v.25 no.4
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    • pp.288-296
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    • 2014
  • The approach of using the structure of the Joseon Dynasty Zen tradition we design a piece of table for outdoor camping is a necessity in modern culture as continuing to develop this research. Analyzing outdoor and camping equipment according to the time change, we focus on the study table for outdoor camping can apply the structure of the elbow. Outdoor supply is also overlaid with the change, while changing in the picnic and camping camp sensibility that was taking advantage of the individuality of each of the general camp. However, it is a surface camp that Korean culture is not to build up the camp culture that has been fixed camp culture of the world in the only development, following the camp trend of European and American. In an attempt to adhere to the trend of Korea currently camped, camping outdoor products that apply the beauty of Korea with a combination of new modern representation tradition and our culture has designed research. I have designed the camp outdoor table based on the contents mentioned above. For the design of this time, it is now possible to use appropriately in accordance with the outdoor environment conditions that are designed to upright seat sharing table in the market tables for camping and to be divided into the seat and upright.

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Proteome Analysis of Waito-c Rice Seedlings Treated with Culture Fluid of Gibberellin-producing Fungus, Fusarium proliferatum KGL0401

  • Rim, Soon-Ok;Lee, Jin-Hyung;Hwang, Seon-Kap;Suh, Seok-Jong;Lee, Jin-Man;Rhee, In-Koo;Kim, Jong-Guk
    • Journal of Microbiology and Biotechnology
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    • v.16 no.12
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    • pp.1990-1994
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    • 2006
  • Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited a high GA productivity. A gas chromatography-mass spectrometry (GC-MS) analysis of extracts of the culture fluid of F proliferatum KGL0401 also revealed the presence of $GA_1$, $GA_3$, $GA_4$, $GA_7$, $GA_{20}$, and $GA_{24}$. Therefore, the present study conducted a proteome analysis of waito-c rice treated with the culture fluid of the isolated F proliferatum KGL0401 to identify the protein expression triggered by the GA-containing culture fluid. The results revealed the overexpression of 180 protein spots in the sample treated with the culture fluid. Among them, 75 induced proteins were selected and analyzed by MALDI-TOF (matrix-assisted laser desorption-iorrization time-of-flight) mass spectrometry, followed by database searching, and 51 proteins were identified.

Ancient Sino-Korea Sea Communication and Ship-cultural Exchange (고대중조해상교왕일\ulcorner반문화교류)

  • ou, Xin-Yuan
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2000.06a
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    • pp.171-183
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    • 2000
  • Regarding the ancient Sino-Kroea sea communication as the main theme, the propagation of the China-culture in Koran Peninsula and he influence of Chinese ancient shipbuilding technique for Korea are described in the paper. Frequent sea trade resulted in the flourishing of Chinese and Korean harbours on both side of Yellow Sea. Sino-Koran sea communication is of long-standing. Korean Peninsula (KP) is just as the large arm extended to the great sea from the North-east China. It is the Chinese mainland plate-lump's extended part in the Yellow Sea. In the ancient time, the land communication between China and Korea was often not unimpeded, the contact between China and KP, such as the South-Korea, depended on the sea communication mainly, Although the friendly relationship between China and Korea rose one after another in the various historical period, but considering from the great background of people's historical development, just as the modern people benefited fro the Europe-American culture, the people of ancient time also benefited from the China-culture. All China, KP and Japan obtained the development by propagating the ancient China-culture. The seagoing ships which carried out the Sino-Korea sea communication and trade, as the friendly envoys, finished the mission linked u and developed the Sino-Korea culture contact.

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CYTOTOXICITY OF D-GALACTOSAMINE ON PRIMARY CULTURES OF ADULT RAT HEPATOCYTES

  • Yang, K.H.;Park, Kwan-Ha;Kim, Byung-Sam
    • Toxicological Research
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    • v.3 no.2
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    • pp.73-80
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    • 1987
  • Primary cultures of adult rat hepatocytes were used to study the cytotoxicity of D-galactosamine. Hepatocytes were isolated by a collagenase perfusion technique and maintained as monolayers in serum-free medium on collagen-coated culture dishes. Treatment of galactosamine to the culture markedly inhibited the uptake of ${\alpha}$-aminoisobutyric acid (AIB) inducible with glucagon and dexamethasone. At0.1 mM of galactosamine, AIB uptake was inhibited significantly when treated for 12 hr. At higher doses (0.25, 0.5 and 1.0mM), a significant inhibition was noticed after 1 hr exposure. Generally the magnitude of the inhibition was related to the dose and treatment time of galactosamine. Treatment of galactosamine also produced a dose- and treatment time-related suppression of the tyrosine aminotransferase (TAT) induction caused by dexamethasone. Meanwhile, uptake of ouabain was not affected by the treatment of galactosamine. The viability of the hepatocytes was decreased only slightly by the treatment of galactosamine; more than 87% of the cells excluded tryphane blue when treated 1 mM galactosamine for 12 hr. Galactosamine induced depressions of AIB uptake and TAT activity were prevented by the simultaneous addition of uridine to the culture. D-Galactosamine, cytotoxicity, hepatocytes culture, ${\alpha}$-aminoisobutyric acid uptake, tyrosine aminotransferase.

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A Study of "Americanization" expressed in Korean Clothing Culture -Through the Magazines from 1920s to 1930s- (한국 근대 복식문화에 나타난 아메리카나이제이션(Americanization)에 관한 연구 -1920년대부터 1930년대까지의 잡지를 중심으로-)

  • 안선경;양숙희
    • Journal of the Korean Society of Clothing and Textiles
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    • v.25 no.1
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    • pp.50-60
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    • 2001
  • This study is to clarify the concept of \"Americanization\" among korean modern clothing culture by investigating magazines from 1920s to 1930s. The Americanization of modern clothing culture can be divided to New fashion, Sports-wear, and Cloth improvement. 1. New fashion, Western culture was the main stream of new fashion, and modern boys and girls could bear cultural privileged consciousness. And the spread of modern culture was so fast that exaggerated fashional preference provoked extravagancy and loss of individuality. 2. Sports-wear. Under the rule of Japanese imperialism, initial sports activity was encouraged to regulate colony by principle and order. Through the sports-wear, practicality of clothing, exposure of female body, and the concept of T.P.O.(Time, place, occasion) were introduced. 3. Cloth improvement. The capitalism and modern concept of \"time\" were introduced and promoted maximum productivity. And to increase productivity, imperialist educated conveniency of western clothes and inconveniency of traditional Korean clothes. These could affect Koreans to think themselves a sense of inferiority(uncivilized complex).ncivilized complex).

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An Empirical Study on the Challenge of Maintaining Knowledge Pieces in KMS(Knowledge Management System) (KMS(Knowledge Management System)내 지식에 대한 유지보수 요청 의향에 관한 실증적 연구)

  • Lee, Ook;Ahn, Jong-Chang
    • Journal of Information Technology Services
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    • v.8 no.1
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    • pp.143-163
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    • 2009
  • The study investigates the challenge of knowledge maintenance in the KMSs. Knowledge pieces are the embodiment of structures in an organization and need to be modified tuned to environmental change over time. Since the change of knowledge in the KMS is not made automatically, it requires user's active participation which is called maintenance action. This study shows that users are not voluntary in taking maintenance action with empirical data based upon knowledge pieces that are already established in the KMS. This article shows that the intention of maintaining KMS is negatively influenced by KM-related culture, organizational culture and the authority of knowledge piece rather than the organizational demography. An organizational culture has an influence directly upon the intention of maintaining knowledge but influence upon KM-related culture or the authority of knowledge piece, the influence indirectly related to the intention of maintaining knowledge. It can be argued that the organizational demography have only meager influence upon the intention of maintaining knowledge only by KM-related culture. This research has the implication that what factors are to be considered in maintaining knowledge pieces over time for the organization managers.

Evaluation of the Selective Enrichment Culture to Recover Clostridium difficile

  • An, Byoungrak;Kim, Heejung;Lee, Kyungwon
    • Korean Journal of Clinical Laboratory Science
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    • v.46 no.4
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    • pp.140-142
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    • 2014
  • To evaluate the recovery rates to increase toxigenic C. difficile, the selective enrichment broth culture methods were compared with commonly used cytotoxin assays and toxigenic culture. First, the enrichment culture, using the selective medium broth for 2 to 5 days, was performed and then, toxigenic C. difficile was confirmed by C. difficile toxin gene-specific PCR after being cultured on C. difficile selective agar. The sensitivity of C. difficile from the enrichment culture (100%) was higher than that of C. difficile selective agar culture (93.8%), while positive predictive values (PPV) were low; 72.7% (16/22) and 88.2% (15/17). PPV of the enrichment culture are not high. Recently, combinations of C. difficile selective agar culture, C. difficile A & B assays, glutamate dehydrogenase, and nucleic acid amplification method are widely used. The enrichment culture was disadvantageous in PPV, turn-around time, and cost. So, what we performed is not considered as a common method of diagnosis of C. difficile-associated diarrhea.

Influence of time pressure on the purchase decision making process in apparel shopping

  • Moon, Ji-Young;Lee, Kyu-Hye
    • The Research Journal of the Costume Culture
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    • v.21 no.1
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    • pp.117-128
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    • 2013
  • Studies show that time pressure affects the purchase decision-making process of consumers. However, in the case of apparel shopping, few studies have looked into the influence of time pressure on the purchase decision-making process. This study aims to determine how perceived time pressure in apparel shopping, time pressure situations, and product type and the interactions between these variables influence the clothing purchase process. An empirical study was conducted among males and females in their 20s and 30s. Perceived time pressure in apparel shopping was measured using four items. Time pressure situations and product types were given in the form of scenarios. A $2{\times}2{\times}2$ experimental design was used, and perceived time pressure in apparel shopping, time pressure situations, and product type were used to create eight different situations. The dependent variables included decision-making speed, anticipated regret, and anticipated purchase satisfaction. Data from 512 subjects were collected through an online data collection. Results showed that the high perceived time pressure group and the shopping situation with time pressure involved a significantly high level of decision-making time, anticipated regret, and anticipated purchase satisfaction. Marketers must understand the real-time pressure situations of consumers.

Comparison of cytokine genes related with immune responses in canine macrophages using different culture models after infection with Brucella canis

  • Park, Woo Bin;Kim, Suji;Shim, Soojin;Yoo, Han Sang
    • Journal of Preventive Veterinary Medicine
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    • v.43 no.4
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    • pp.214-220
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    • 2019
  • Although canine brucellosis has been known to be an important re-emerging zoonosis, the pathophysiological mechanisms of Brucella canis infection remains clues to be solved. Different culture models, single and co-culture models, were constructed with canine epithelial cells, D17 and macrophage, DH82 to investigate the induction of immune responses in in vivo B. canis infection. Expression of genes related with induction of immune responses, Th1, Th2 and Th17, was compared in the two different models after the bacterial infection. In this study, expression of cytokine genes, IL-1β, IL-5, IL-6, IL-10, IL-23, and TNF-α was quantified in the DH82 at different time points using RT-qPCR in the two different culture systems after the infection. Cytokine genes related with Th1, IL-1β and TNF-α and Th17, IL-6 and IL-23 were expressed with time-dependent manners in the both systems (p<0.05). However, increase of Th2-related cytokine genes expression was not detectable in the both systems by comparison with control. The expression of Th1 and Th17 related cytokine genes was earlier in single cell culture than those in co-culture model (p<0.05). In general, amounts of the expressed genes were shown higher in single cell model than those in co-culture models. This study indicate that Th1 and Th17-associated immune responses are central to B. canis infection in dogs. In addition, it suggests a specific role of epithelial cells in the B. canis infection in vivo, which should resolved in the further study.

Real Time Electrical Energy Computing Tool

  • Kumpanya, Danupon;Thaiparnat, Sattarpoom
    • International Journal of Advanced Culture Technology
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    • v.3 no.1
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    • pp.113-119
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    • 2015
  • This paper presents a design and implementation of real time electrical energy computing tool to measure and record the electrical energy based on type of detection devices, Hall Effect current sensor and Microcontroller. The tool was installed on the system power supply of the room and compared with kWh meter. Finally, we found that the energy record has error of average power calculating results is 0.077%.