• Journal of Microbiology
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• 제45권2호
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• pp.122-127
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• 2007

Factors indicating culture status of two Spirulina platensis strains were monitored in a batch mode cultivation for 36 days. Changing mode in all factors showed a common turning point, indicating shift of cell or culture status. Mean biomass productivity was highly sustained until day 22, chlorophyll a concentration peaked on day 22, pH value was > 12 on day 22, coil number was abruptly shortened on day 22, and floating activity was sustained at greater than 79% after day 22, indicating that day 22 is a criterion reflecting phase-transfer in cell physiology in a batch culture system. Many of these changes may have been caused by increased pH, suggesting that pH control is essential for mass production of S. platensis. Fluctuations in floating activity were likely induced by the number of cellular gas vacuoles. Consequently, coil number per trichome and floating activity of S. platensis could readily act as simple indicators for determination of culture status or harvesting time of cells.

• 한국안전학회지
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• 제30권4호
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• pp.174-180
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• 2015

This study developed a Safety Culture Impact Assessment Model (SCIAM) which consists of a safety culture assessment methodology and a safety culture impact quantification methodology. The SCIAM uses safety culture impact index (SCII) to monitor the status of safety culture of the NPPs periodically and it uses relative core damage frequency (RCDF) to present the impact of safety culture on the safety of the NPPs. As a result of applying SCIAM to the reference plant (Kori 3), the standard for the healthy safety culture of the reference plant is suggested. SCIAM might contribute to improve the safety of the NPPs (Nuclear Power Plants) by monitoring the status of safety culture periodically and presenting the standard of healthy safety culture.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.980-987
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• 2008

The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The developmental rates beyond morula stage in the BOEC co-culture group was significantly higher than in the control group (p<0.05). In particular, blastocyst production in the BOEC co-culture group (28.3%) was dramatically increased compared with the control group (7.2%). In the in vitro development of bovine IVM/IVF embryos according to cell status, the developmental rates beyond morula stage in the primary culture cell (PCC) co-culture group were the highest of all experimental groups. Expression of genes related to growth (TGF-${\beta}$ EGF and IGFBP), apoptosis (Bax, Caspase-3 and p53) and antioxidation (CuZnSOD, MnSOD, Catalase and GPx) in different status cells of BOEC for embryo culture was detected by RT-PCR. While EGF gene was detected in isolated fresh cells (IFC) and PCC, TGF-${\beta}$ and IGFBP were found in IFC or PCC after use in the embryo culture, respectively. Caspase-3 and Bax genes were detected in all experimental groups regardless of whether the BOEC was used or not used in the embryo culture. However, p53 gene was found in IFC of both conditions for embryo culture and in frozen/thawed culture cells (FPCC) after use in the embryo culture. Although antioxidant genes examined were detected in all experimental groups before using for the embryo culture, these genes were not detected after use. This study indicated that the BOEC co-culture system used for in vitro culture of bovine IVF embryos can increase the developmental rates, and cell generations and status of BOEC might affect the in vitro development of bovine embryos. The BOEC monolayer used in the embryo culture did not express the growth factors (TGF-${\beta}$ and EGF) and enzymatic antioxidant genes, thereby improving embryo development in vitro.

• Clinical and Experimental Reproductive Medicine
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• 제21권1호
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• pp.77-81
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• 1994

To improve in vitro embryonic cell development, this study was desigend to culture in vitro fertilized early embryos of mouse in two different systems; conditioned medium alone and ampullary cells co-culture. Thirty two of 83 embryos(38.6%) were blocked in the 2 cell stage by co-culture, as compared to forty of 42 embryos(95.2%) in control group for 24hours culture. And all the embryonic cells cultured for conditioned medium alone were blocked for 48 hours culture. Twenty seven of 46 embryos (58.7 %) which overcome culture block in 2 cell stage by cocultured were developed morular and expanded blastocyst, and ninteen of 46 embryos(26.1 %) underwent hatching for 96 hours culture. The cellular fragmented rates for embryo were 26.2% in medium alone; 10 fragmented blastomere were graded mild status and 1 fragmented blastomere in severe status. On the other hand, the fragmented rate for 48 hours co-cultured were 15.7%03/83); 8 fragmented embryos were graded mild status, moderate status in 3 fragmented embryos and severe in 2 fragmented embryos respectively. In conclusion, the co-culture of embryos with ampullary cells is good to improve quality of embryos and overcome of culture block as well as development of cell cleavage.

• 복식문화연구
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• 제19권2호
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• pp.416-429
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• 2011

Most of precedent studies related to hip hop culture and fashion, which have been conducted till now were progressed under only theoretical background of hiphop, not the changed status of hiphop currently. Practical studies explaining the status of hip hop in popular culture, especially, the area of popular fashion are insufficient. Therefore, this study was conducted by focusing on cultural characteristics and musical characteristics by the stream of period, for which concept hip hop is accepted to people currently and which effect it makes on the area of fashion. Especially, the study was progressed by dividing periodical background into before and after the 2000s because there was the most noticeable change for the genre of hip hop in that period. Because of the appearance of 'Alternative hip hop' which is a sub-genre of hip hop music, hip hop music was changed to be more popular. Also, hip hop fashion also showed big changes. Hip hop fashion before 2000s had showed poor conditions, resistance, complex and alienation of them by their fashion. On the other hand, hip hop fashion after 2000s also showed popular characteristics because of high-level environment and social status. This study intended to understand and grasp hip hop as a kind of cultural situation coexisted by many-sided characteristics as well as redefine the changed concept and contents of new hip hop currently.

• 한국학교ㆍ지역보건교육학회지
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• 제20권3호
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• pp.43-52
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• 2019

Objectives: This study aims to understand the effects of job stress and emotional labor on perceptive health status of dental hygienist's. And provide basic data by for establishing the plan for the effective management of job stress and emotional labor and the promotion of health. Methods: Self-evaluation questionnaires were surveyed for 441 dental hygienist's who agreed to participate in the survey, using questionnaires used in the preceding study were modified and complemented. We analyzed the data with frequency analysis, descriptive statistics, t-test, chi-square test와 ANOVA, Spearman correlation coefficient by using IBM SPSS Statistics 21.0 (IBM Co., Armonk, NY, USA). The significance level for significance was set at 0.05. Results: health status were job stress, emotional labor significant negative correlation. The factors having significant effects on health problem were physical health status, the job culture of job stress, lack of job autonomy, organizational instability, and workplace culture. In addition, social health status was affected by emotional expressionism, relationship conflict, organizational instability, organizational system, and workplace culture. Conclusion: Recognizing the importance of the management of job stress and emotional labor in the working environment to dental clinic and hospital managers and managerial dental hygienists, a dental hygienist health promotion intervention program is needed to mitigate and cope with job stress and emotional labor.

• 한국도서관정보학회지
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• 제45권1호
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• pp.297-317
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• 2014

본 연구의 목적은 국가산업단지의 하나인 경기도 시흥시에 위치한 시흥스마트허브단지내 근로자의 삶의 질 향상과 기업경쟁력을 강화하기 위해 독서환경, 독서문화 실태 및 요구사항을 조사, 분석하여 독서문화 개선 및 활성화를 위한 기초자료를 삼고자 하였다. 산업체 근로자를 대상으로 설문을 하여 그 결과를 분석한 결과 산업단지 내 독서환경이 미비하였으며, 근로자의 독서문화 실태도 열악한 상황이었다. 이를 개선하기 위해 요구사항을 분석하여 산업체 근로자의 독서환경 개선과 독서문화 활성화를 위한 방안을 제시하였다.

• 한국산림과학회지
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• 제95권2호
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• pp.194-202
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• 2006

The purpose of this study was to provide necessary requirements on accreditation for forest culture and recreational interpretive programs in order to set up the enforcement regulations of the Forest Culture and Recreation Act. The intention of this study was to contribute to raising the qualitative standards of forest interpreters through the adoption of accreditation procedures for forest culture and recreational education programs, to establish detailed standards regarding the accreditation of forest interpreter training courses, and to provide materials and resources useful in creating forestry policies regarding forest interpreters and forest culture and recreation. This study examined five topics: 1) To establish conceptual understanding of the accreditation procedures for forest culture and recreation interpretive programs, 2) To understanding the present status of training programs and courses related to accreditation procedures for forest culture and recreation interpretive programs, and examining the status of current forest interpreter training courses, 3) To propose an operational scheme for accreditation procedures for forest culture and recreation programs, and develop the goal of an operation of the accreditation procedures for forest culture and recreation programs, 4) To propose an operational scheme for accreditation procedures for forest culture and recreational education programs, and the operational scheme for forest interpreter training courses, and 5) To establishing a commission to evaluate the accreditation procedures for forest culture and recreation programs and operating guidelines.

• 한국도서관정보학회지
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• 제34권4호
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• pp.127-151
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• 2003

본 연구에서는 서울시 24개 공공도서관의 문화 프로그램과 독서 활동의 실태를 파악하고 활성화 방안을 제시하고자 한다. 선행된 연구를 통해 공공도서관의 문화 프로그램 및 독서 활동에 대한 이론적 근거를 살펴보고 본 연구의 대상이 되는 서울시 24개 공공도서관의 문화 프로그램과 독서 활동의 실태를 조사 분석한다. 또한 새로운 문화 패러다임의 모델로 노원어린이도서관 독서활동 특성화 프로젝트를 소개한다.

• 복식문화연구
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• 제10권4호
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• pp.441-460
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• 2002

Funeral culture, which came to being along with the death of human beings, has developed through many changes in the background, culture, religion and custome of the times, having variant cultures depending on each nation or era. This study is designed to historically and systematically classify funeral rituals and costumes which have constantly changed in a special funeral culture from ancient times to modern times so as to investigate the features of each age. The researcher worked on Western funeral cultures, focusing on Egypt and Rome of ancient times, Creek times, the Middle Ages, recent and modern times ages, referring to literature, precedent studies, domestic and international technical books, pictures and drawings in relation to death and funeral services. Western funeral rituals were designed for offering condolence to the dead, but also used to show off the status of the mourners and the position of the dead persons. The mourning dress were utilized to indicate mourning in accordance with the colors, materials and the ways of wearing them, serving as a vehicle for showing off one's own status.