• Journal of Animal Science and Technology
• /
• 제58권5호
• /
• pp.21.1-21.8
• /
• 2016

Background: The aim of the present study was to determine the effects of feeding trace mineralsfortified mixed microbial culture (TMC) on ruminal fermentation, nutrient digestibility, blood electrolyte status, nitrogen balance, and trace mineral balance in sheep. Methods: Mixed microbes [0.6 % (v/w) of Enterobacter sp., Bacillus sp., Lactobacillus sp., and Saccharomyces sp.] were cultured with 99 % feedstuffs and 0.4 % trace minerals including zinc and copper for ensiling. Six sheep (a mean body weight of $46.5{\pm}1.2kg$) were fed two diets: a control diet (concentrate mix and rye straw) and an experimental diet (a control diet + 3.1 % TMC). Results: TMC feeding did not induce negative effects on ruminal fermentation, nutrient digestibility, blood electrolytes, and nitrogen balance in sheep. Feeding with TMC increased the intake of trace minerals (p < 0.05) and did not affect absorption of trace minerals in the whole digestive tract. Feeding with TMC increased fecal excretion and absorbable intake, and retention of zinc and copper (p < 0.05) by 71 % and 77 %, respectively. Conclusion: Feeding with TMC resulted in higher zinc and copper bioavailability and retention without any adverse effects on sheep performance.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권4호
• /
• pp.428-433
• /
• 1998

The effects of chromium picolinate supplementation in pig diet were evaluated by measuring the in vitro lipogenic and lipolytic activities in adipose tissue and the protein synthetic activity in liver acinar cell in culture. Thirty-two male and thirty-two female pigs were randomly assigned to one of four dietary groups: Control, 100 ppb, 200 ppb, and 400 ppb of Cr in the form of picolinate. The chromium picolinate supplementation (p < 0.01) increased the in vitro lipolytic activity in adipose tissue of pig, but had no effects on lipogenesis. The chromium picolinate effect was greater in female pigs than in male pigs on lipolytic activity. The results from the studies with the liver acinar cells in culture indicated that chromium picolinate supplementation increased protein synthetic activity (p < 0.05). It was observed through this experiment that chromium picolinate functions not only on fat degradation but also on retained protein synthesis.

• 한국수정란이식학회:학술대회논문집
• /
• 한국수정란이식학회 2000년도 추계학술대회 및 정기총회
• /
• pp.66-67
• /
• 2000

• 복식문화학회:학술대회논문집
• /
• 복식문화학회 2000년도 International Costume Culture Conference
• /
• pp.71-71
• /
• 2000

• International Journal of Advanced Culture Technology
• /
• 제8권1호
• /
• pp.141-151
• /
• 2020

The purpose of this study is to investigate the positive and negative effects of college student part-time experience on college life. The subjects were 335 college students in Chungcheongnam - do. The data were collected by the self - study questionnaire on general characteristics, part - time experience, university life. There is more positive viewpoints in the Area of Public Health than in other majors and in the third grade and it shows that the higher the grade it is, the more positive viewpoints there is. The longer the period of doing part-time work it is, the more negative viewpoints about the life in the university there is, and it needs to be paid attention to. The factors which affects to the positive viewpoints about the life in the university which needs to be paid attention to was the grade and marks, and the negative viewpoints about the life in the university which needs to be paid attention to was marks, economic status, and the period of doing the part-time work. The factors which affects to the positive viewpoints and the negative viewpoints, there needs to be the clear and detailed education, counselling, and welfare, and based on the conclusion of this research, there needs to be a practical solution to improve the life of the university of the students who are experiencing part-time work.

• Clinical and Experimental Reproductive Medicine
• /
• 제41권1호
• /
• pp.1-8
• /
• 2014

Objective: Estrogen related receptor ${\beta}$ (Esrrb) is a member of the orphan nuclear receptors and may regulate the expression of pluripotencyrelated genes, such as Oct4 and Nanog. Therefore, in the present study, we have developed a method for delivering exogenous ESRRB recombinant protein into embryos by using cell-penetrating peptide (CPP) conjugation and have analyzed their effect on embryonic development. Methods: Mouse oocytes and embryos were obtained from superovulated mice. The expression of Oct4 mRNA and the cell number of inner cell mass (ICM) in the in vitro-derived and in vivo-derived blastocysts were first analyzed by real time-reverse transcription-polymerase chain reaction and differential staining. Then 8-cell embryos were cultured in KSOM media with or without $2{\mu}g/mL$ CPP-ESRRB protein for 24 to 48 hours, followed by checking their integration into embryos during in vitro culture by Western blot and immunocytochemistry. Results: Expression of Oct4 and the cell number of ICM were lower in the in vitro-derived blastocysts than in the in vivo-derived ones (p<0.05). In the blastocysts derived from the CPP-ESRRB-treated group, expression of Oct4 was greater than in the non-treated groups (p<0.05). Although no difference in embryonic development was observed between the treated and non-treated groups, the cell number of ICM was greater in the CPP-ESRRB-treated group. Conclusion: Treatment of CPP-ESRRB during cultivation could increase embryos' expression of Oct4 and the formation rate of the ICM in the blastocyst. Additionally, an exogenous delivery system of CPP-conjugated protein would be a useful tool for improving embryo culture systems.

• 문화기술의 융합
• /
• 제9권1호
• /
• pp.557-564
• /
• 2023

본 연구는 간호대학생을 대상으로 지각된 스트레스, 대인관계능력, 대학생활적응 간의 관계를 파악하고, 지각된 스트레스와 대학생활적응과의 관계에서 대인관계능력의 매개효과를 확인하기 위해 시도되었다. 연구참여자는 B시의 간호대학생 281명을 대상으로 하였다. 자료분석은 SPSS 23.0 프로그램을 이용하여 실수, 백분율, 평균, 표준편차, t-test, ANOVA, 피어슨 상관계수, 다중회귀분석, sobel test로 분석하였다. 대상자의 지각된 스트레스 평균 2.62±0.62점, 대인관계능력 평균 3.74±0.62점, 대학생활적응 평균 3.38±0.58점이었다. 대상자의 대학생활적응은 지각된 스트레스(r=-.478, p<.001)와 부적상관관계, 대인관계능력(r=.617, p<.001)과 정적상관관계를 나타냈다. 대상자의 지각된 스트레스와 대학생활적응과의 관계에서 대인관계능력이 부분매개효과(β=.629, p<.001)를 가지는 것으로 확인되었다. 따라서 간호대학생의 대학생활적응을 높이기 위하여 지각된 스트레스를 줄이고 대인관계능력을 높일 수 있는 교육 및 프로그램의 개발이 필요하다.

• 한국환경과학회지
• /
• 제23권5호
• /
• pp.895-902
• /
• 2014

Bacterial cellulose (BC) has played important role as new functional material for food industry and industrial products based on its unique properties. The interest in BC from static cultures has increased steadily in recent years because of its potential for use in medicine and cosmetics. In this study, we investigated culture condition for BC production by Acetobacter sp. F15 in static culture. The strain F15, which was isolated from decayed fruit, was selected on the basis of BC thickness. The optimal medium compositions for BC production were glucose 7%, soytone 12%, $K_2HPO_4$ 0.2%, $NaH_2PO_4{\cdot}_2H_2O$ 0.2%, lactic acid 0.05% and ethanol 0.3%, respectively. The strain F15 was able to produce BC at $26^{\circ}C-36^{\circ}C$ with a maximum at $32^{\circ}C$. BC production occurred at pH 4.5-8 with a maximum at pH 6.5. Under these conditions, a maximum BC thickness of 12.15 mm was achieved after 9 days of cultivation; this value was about 2.3-fold higher than the thickness in basic medium. Scanning electron micrographs showed that BC from the optimal medium was more compact than plant cellulose and was reticulated structure consisting of ultrafine cellulose fibrils. BC from the optimal medium was found to be of cellulose type I, the same as typical native cellulose.

• Journal of Microbiology and Biotechnology
• /
• 제16권3호
• /
• pp.374-380
• /
• 2006

In this study, glucose, sucrose, and dextrin were found to be better carbon sources for the production of pullulan by Aureobasidium pullulans HP-2001. Maximal production of pullulan with 200 g/l sucrose as a carbon source was 54.2 g/l. The highest yield of pullulan from sucrose was 0.40, when the sugar concentration was 100 g/1. Optimal conditions for the continuous production of pullulan by A. pullulans HP-2001 in a 7-1 bioreactor were determined by studying the effects of composition of feed solution, dilution rate, and concentration of sucrose in the feed solution. Pullulan concentration and productivity with 100 g/l glucose and 2.5 g/l yeast extract were 38.1 g/l and 0.53 g/l h for 72 h, respectively, in a batch culture of A. pullulans HP-2001. When the substituted medium contained 100 g/l sucrose, 2.5 g/l yeast extract, and mineral salts, which is the same composition as the medium for the production of pullulan, the pullulan concentration and productivity were 74.9 g/l and 0.55 g/l h for 120 h, respectively. The production of pullulan at the steady state increased with a dilution rate up to 0.015/h, and its concentration was 78.4 g/l with a weight average molecular weight ($M_w$) of $4.0{\times}10^5$. Unlike a batch culture, however, the decline of the $M_w$ and the number average molecular weight ($M_n$) of pullulan was not found in the continuous culture of A. pullulans HP-2001. When the concentration of sucrose in the feed solution was 200 g/l, 113.5 g/l of pullulan was obtained at the steady state. The steady state was maintained longer in the continuous culture fed with the feed solution containing 200 g/l sucrose than when fed with the feed solutions containing either 100 or 150 g/l sucrose.

• 한국약용작물학회지
• /
• 제5권3호
• /
• pp.186-190
• /
• 1997

This study was carried out to investigate the effect of polyamines, salt strength. sucrose and gelling agents on the regeneration of plantlets by meristem culture of Aloe arborescens Mill. and Aloe vera L.. Shoot multiplication was more effective when 10mg/ l spermine in Aloe arborescens and 1mg/ l spermidine in Aloe vera added into MS medium than when other polyamines were treated into media. A quarter strength of MS medium was effective for rooting of shoots regenerated. Higher concentration of sucrose (45g/ l) was more effective for shoot regeneration. Addition of 4g/ l gelrite into the medium was effective for induction of multiple shoots from Aloe than that of agar or other concentrations of gelrite. When plantlets regenerated from meristem culture were transferred to pot. survival rate of plantlets was 80% on perlite and was 95% on vermiculite. respectively.