• Title/Summary/Keyword: culture method

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A Study on Application for Dress Form Made from a Replica Method - A Comparison of the Satisfaction of Fit of Basic Dresses Using the Draping and Flat Pattern Methods

  • Shim Kue-Nam
    • The International Journal of Costume Culture
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    • v.8 no.2
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    • pp.97-104
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    • 2005
  • In this research, the fit of basic dresses was examined, by analyzing and comparing the satisfaction of fitness of basic dresses made by the draping method on a custom-made replica body form and that of a basic dress made by the flat pattern method. Basis dresses were produced by the draping method for each 52 subjects using custom-made body forms, and another kind basic dresses for each subjects were produced by the flat pattern method using measurements of body sizes. Both basic dresses received high scores for the satisfaction of fit, but the basic dresses produced by draping method received higher scores. Thus it is expected to produce a more successful fit by draping a basic dress on a custom-made body form. Future research using more subjects might confirm these results. A more concrete analysis through using additional satisfaction tests could be conducted. For example, each subject might also serve as an evaluator to rate her satisfaction of fit wearing each of the two custom-made dresses. Finally, examining various body forms is suggested.

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Wavelet Transform based Image Registration using MCDT Method for Multi-Image

  • Lee, Choel;Lee, Jungsuk;Jung, Kyedong;Lee, Jong-Yong
    • International Journal of Internet, Broadcasting and Communication
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    • v.7 no.1
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    • pp.36-41
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    • 2015
  • This paper is proposed a wavelet-based MCDT(Mask Coefficient Differential and Threshold) method of image registration of Multi-images contaminated with visible image and infrared image. The method for ensure reliability of the image registration is to the increase statistical corelation as getting the common feature points between two images. The method of threshold the wavelet coefficients using derivatives of the wavelet coefficients of the detail subbands was proposed to effectively registration images with distortion. And it can define that the edge map. Particularly, in order to increase statistical corelation the method of the normalized mutual information. as similarity measure common feature between two images was selected. The proposed method is totally verified by comparing with the several other multi-image and the proposed image registration.

Quality Characteristics of Frozen Soy Yogurt Prepared with Different Proteolytic Enzymes and Starter Culture (단백분해효소 전처리 및 starter culture의 종류에 따른 frozen soy yogurt의 품질특성)

  • Lee, Jung-Eun;Lee, Sook-Young
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.676-681
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    • 2001
  • This study was carried out to evaluate the quality characteristics of frozen soy yogurts prepared with different proteolytic enzymes and starter culture. The viable cell counts of lactic acid bacteria in frozen soy yogurts were measured $10^8$ CFU/g by the single culture method, while $10^9$ CFU/g by the mixed culture method except ${\alpha}-chymotrypsin$ treatment. The viable cell counts of lactic acid bacteria did not decrease after freezing for 30 min in ice cream maker. The lactic acid bacteria from the mixed culture showed better bile acid tolerance than those from the single culture. The lactic acid bacteria from the frozen soy yogurt prepared with ${\alpha}-chymotrypsin$ and mixed culture of Bifidobacterium bifidum and Lactobacillus bulgaricus showed better acid tolerance and bile acid tolerance. The highest(73.45%) overrun was observed in the frozen soy yogurt treated with ${\alpha}-chymotrypsin$ and mixed culture of B. bifidum and L. bulgaricus. The melt-down percent was higher in the single culture than the mixed culture. In sensory test, the frozen soy yogurt prepared with ${\alpha}-chymotrypsin$ and mixed culture of B. bifidum and L. bulgaricus was the most desirable, the highest scores in sourness, bitterness and mouthfeel.

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Double-culture Method Enhances the in Vitro Inhibition of Atopy-inducing Factors by Lactococcus lactis (이중배양법에 따른 Lactococcus lactis의 아토피 유발인자 억제 효과 증대)

  • Jo, Yu Ran;Kang, Sang Mo;Kim, Hyun Pyo
    • Journal of Life Science
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    • v.25 no.7
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    • pp.810-818
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    • 2015
  • We analyzed whether lactic acid bacteria could control the expression of IL-4 and IL-13 in activated mast cells and whether these bacteria could inhibit the activity of transcription factors such as GATA-1, GATA-2, NF-AT1, NF-AT2, and NF-κB p65. We previously described a technique for identification of lactic acid bacteria with anti-atopy functionality by confirming increased expression of CD4+/CD25+/foxp3+ in T cells. We also confirmed that a double-culture method increased the antibacterial activity of these lactic acid bacteria against Staphylococcus aureus (S. aureus). In the present study, we characterized the effect of lactic acid bacteria cultured by this double-culture method on inhibition of allergic inflammatory reactions of RBL-2H3 mast cells, a cellular model of atopic dermatitis. The strongest anti-allergic effects of the lactic acid bacteria were seen in the following order: Lactococcus lactis broth cultured with medium containing Lactobacillus plantarum culture supernatant > Lc. lactis > Lc. lactis broth cultured with medium containing Lb. plantarum culture supernatant > Lb. plantarum. Thus, Lc. lactis cultured in medium containing Lb. plantarum culture supernatant had the strongest inhibitory effect on the differentiation of mast cells during allergic reactions, which may be mediated through the selective regulation of expression of relevant genes.

Evaluation of Nutrients during Rack and Bag Culture or Suspended Culture of Pacific Oyster Crassostrea gigas (부유망식과 수하식 양성방법에 따른 참굴(Crassostrea gigas)의 영양상태)

  • Choi, Yong-Jun;Tri, Nguyen Thanh;Lee, Jeong-Mee;Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.50 no.3
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    • pp.263-269
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    • 2017
  • The mineral contents, and free amino acid, and fatty acid composition of rack and bag or suspended cultured oysters Crassostrea gigas were compared. The moisture content did not change much from 82.8% to 86.3% in the different culture methods. The glycogen level was low 4.7% during rack and bag culture but high 7.2% during suspended culture. The sodium level was the highest during suspended culture, while copper and zinc level were highest during rack and bag culture. The taurine level was 1,385-1,375 mg/100 g in oyster cultured using the rack and bag method, but that in the market sample was lowest at 1,015.8 mg/100 g. Hydroxyproline, glutamic acid, proline, glycine, and alanine were detected at high levels in the experimental oysters. The polyunsaturated fatty acid content was similar among samples. The docosahexaenoic acid level was 14% with rack and bag culture and 12% with suspended culture, whereas the eicosapentaenoic acid level was 15% with rack and bag culture and 20% with suspended culture. The atherogenic index (AI), thrombogenicity index (TI), and hypocholesterolemic/ hypercholesterolemic (h/H) ratio were important factors in the nutritional evaluation. The AI and TI values were 0.5-0.2, and the h/H ratio were 2.0 and 2.4 with the rack and bag and suspended culture, respectively.

The Effect of Organizational Culture of Large Construction Companies on Emotional Commitment through Organizational Trust (건설대기업의 조직문화가 정서적 몰입에 미치는 영향 : 조직신뢰의 매개효과를 중심으로)

  • Kim, Hae-jin;Hwang, Chan-kyu
    • Journal of Venture Innovation
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    • v.6 no.2
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    • pp.83-99
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    • 2023
  • The purpose of this study is to research the impact of the organizational culture of large construction companies on emotional commitment through organizational trust targeting the headquarters and field workers of domestics. Applied the significance sampling method, and the number of samples used in the final analysis was 201, and statistical analysis used the SPSS 20.0 program for the input data processing. As a result of the analysis, first, in the group of the sub-factors of organizational culture recognized by members of large construction companies, rational culture, development culture, and consensus culture had a significant positive (+) effect on emotional commitment, but hierarchical culture was not significant. Second, development culture, consensus culture, and hierarchical culture were found a significant positive (+) effect on organizational trust, but rational culture was not found to be significant. Third, organizational trust was found a significant positive (+) effect on emotional commitment. Fourth, organizational trust has been shown to mediate the relationship between organizational culture and emotional commitment. This suggests that organizational culture that aiming flexibility & change value rather than stability & control value for organizational members can have a positive effect on emotional commitment.

The Tendency Analysis of Korean Traditional Textile Studies (한국 전통 직물 연구의 경향 분석)

  • Choi, Seung-Yeun
    • The Research Journal of the Costume Culture
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    • v.19 no.1
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    • pp.19-30
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    • 2011
  • The purpose of this study is to analyze the tendency of Korean traditional textile studies to review the studies critically and to suggest the new directions. To do this, this study included 164 articles of Korean traditional textile studies published in five Korean major journals from the first issue to the 2009. The identified 164 articles were categorized and analyzed by five areas : study subject, study content, study method, the tendency through times and journals publishing Korea traditional textile articles. The results were as follows. First, in the study subject, the ratio of traditional motifs study was the highest in any other study subject and an actual textile pattern study and society culture historic study were also favored. And, the ratio of foreign textile study was the lowest in any other study subject. Second, in the fiber type of study content, the ratio of silk fiber was the highest in any other fiber type. Hemp, ramie and cotton fiber were followed by silk fiber. Also, the ratio of wool fiber was lowest in any other fiber type. In the weaving type, ratio of plain weave was the highest in any other weaving type. In the times of study, the ratio of the Joseon Dynasty period was the highest in any other times of study. Third, in the study method, the ratio of an actual proof study method was higher than that of literature study method. In the tendency of times, there were society culture historic studies from the time publishing the first edition in each journal, the ratio of an actual textile pattern study, traditional motif study and preservation science study has increased since the 2000. Fourth, in the characteristics of journals, Journal of Korean Society of Costume has published the most articles in any other journal publishing Korea traditional textile study.

Cell Co-culture Method by Patterned Gratt of Thermo-Responsive Polymer (온도응답성 고분자의 패턴상 그래프트를 이용한 공배양법)

  • Bae Jin Suk;Ahn Chang Hyun;Yoon Kwan Han;Kwon Oh Hyeong;Kang Inn-Kyu;Yamato Masayuki;Kikuchi Akihiko;Okano Teruo
    • Polymer(Korea)
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    • v.29 no.3
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    • pp.294-299
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    • 2005
  • Thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) was covalently patterned by masked el electron beam irradiation. Introduction of PIPAAm on tissue culture polystyrene dish was confirmed by ATR-FTIR and ESCA measurements. Hepatocytes were cultured at $37^{circ}C$ on these surfaces. Cells adhered on PIPAAm-grafted domains were detached by reducing culture temperature to $20^{circ}C$. Endothelial cells were then seeded and cultured on the same surfaces. Seeded endothelial cells were selectively attached on hepatocytes detached and PIPAAm-grafted domains and could be co-cultured with hepatocytes on the same culture dishes with clear pattern. This co-culture method enabled long-term co-culture of hepatocytes with endothelial cells.

Morphological Changes in the Mammary Organ Culture of the Rat Treated with 7,12-Dimethylbenz[$\alpha$]anthracene and N-methyl-N-nitrosourea (7,12-Dimethylbenz[$\alpha$anthracene 및 N-methyl-N-nitrosourea를 투여한 랫드 유선 조직 배양에 대한 형태학적 변화)

  • 문지영;정자영;김옥희;이형환
    • Toxicological Research
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    • v.16 no.4
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    • pp.275-284
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    • 2000
  • The organ culture model of the whole mammary gland has many advantages for the study of branching morphogenesis and biological characteristics, including tumorigenesis. Prior to whole gland organ culture, rats were treated with 7,12-dimethylbenz[a]anthracene (DMBA) or N-methyl-N-nitrosourea (MNU) for one week. The tramdorming effect and the morphological changes were assessed by the whole mount preparations and histopathological examination in terminal end buds (TEB), terminal ducts (TD), alveolar buds (AB), alveolar lobules (AL) and hyperplastic alveolar nodules (HAN) of the mammary gland. Grossfindings of the mammary glands at dissection were higher branching morphogenesis and larger volume in carcinogen-treated groups than in carcinogen-non-treated groups. Results of the whole mount method were coincided with those of the histopathological observations. Circular TEB, normally maintained AB, AL, and high cellular density were more frequently observed in carcinogen-treated groups than in carcinogen-nan-treated groups. Histopathologically, as a preneoplastic marker, HAN was maintained only in mammary organ culture of the carcinogen-treated groups. These findings suggest that in vivo trans-formation effects by carcinogens persisted during the mammary organ culture. These results were more characteristic in DMBA than in MNU-treated group. Ducts and terminal ducts appeared to have lost morphology during their growths in case of without diethylstilbestrol (DES). The fact that in vitro organ culture without DES was resulted in abnormal ductular morphogenesis confirms that DES is a physiological regulator of ductular epithelial cell growth.

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The Rapid Drug Susceptibility Testing of Mycobacterium tuberculosis by GenoType$^{(R)}$ MTBDRplus in Contaminated Specimen

  • Heo, Reun;Kim, Yoon-Sik
    • Biomedical Science Letters
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    • v.19 no.4
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    • pp.330-337
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    • 2013
  • There are several methods currently being used to diagnose tuberculosis in patients, such as smear, PCR, tuberculosis culture and X-ray. For a proper medical treatment, antimicrobial susceptibility test and rapid drug susceptibility testing have been operated. Tuberculosis bacilli usually need 3~8 weeks of culture period because of delay in RNA synthesis and require 15~22 hours for generation. After a germ raises in culture, we initiated antimicrobial susceptibility test for a proper treatment. It has some difficulties to give a proper prescription for a tuberculosis patient because antimicrobial susceptibility test requires 4 weeks. To supplement this, we are practicing drug susceptibility testing which allow us to know the sensibility of RMP and INH after 2 or 3 days. But this is only possible when more than 2 positive germ. Therefore, we should practice rapid drug susceptibility testing with culture test. But if media is contaminated by other germs except Mycobacterium tuberculosis, it's hard to interpret result about culture test and to practice antimicrobial susceptibility test and rapid drug susceptibility testing. Because we have to practice again smear, culture test after extracting specimen from the patient, time is consumed and proper patient treatment is postponed. To address these problems and quick patient treatment, rapid drug susceptibility testing is practiced by using GenoType$^{(R)}$ MTDRplus method. As a result of this method we detected sensibility 10 and 7 cases and resistance 0 and 3 cases using RIM and INH respectively with other 1 case toward medicals out of the total 11 test. In conclusion rapid drug susceptibility testing can be used from the contaminated specimen after elimination of contaminated source from culture and proved that it can be practiced for rapid examination of a tuberculosis patient.