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http://dx.doi.org/10.5352/JLS.2015.25.7.810

Double-culture Method Enhances the in Vitro Inhibition of Atopy-inducing Factors by Lactococcus lactis  

Jo, Yu Ran (Department of Biological Engineering, Konkuk University)
Kang, Sang Mo (Department of Biological Engineering, Konkuk University)
Kim, Hyun Pyo (Department of Biomedical Science, Jungwon University)
Publication Information
Journal of Life Science / v.25, no.7, 2015 , pp. 810-818 More about this Journal
Abstract
We analyzed whether lactic acid bacteria could control the expression of IL-4 and IL-13 in activated mast cells and whether these bacteria could inhibit the activity of transcription factors such as GATA-1, GATA-2, NF-AT1, NF-AT2, and NF-κB p65. We previously described a technique for identification of lactic acid bacteria with anti-atopy functionality by confirming increased expression of CD4+/CD25+/foxp3+ in T cells. We also confirmed that a double-culture method increased the antibacterial activity of these lactic acid bacteria against Staphylococcus aureus (S. aureus). In the present study, we characterized the effect of lactic acid bacteria cultured by this double-culture method on inhibition of allergic inflammatory reactions of RBL-2H3 mast cells, a cellular model of atopic dermatitis. The strongest anti-allergic effects of the lactic acid bacteria were seen in the following order: Lactococcus lactis broth cultured with medium containing Lactobacillus plantarum culture supernatant > Lc. lactis > Lc. lactis broth cultured with medium containing Lb. plantarum culture supernatant > Lb. plantarum. Thus, Lc. lactis cultured in medium containing Lb. plantarum culture supernatant had the strongest inhibitory effect on the differentiation of mast cells during allergic reactions, which may be mediated through the selective regulation of expression of relevant genes.
Keywords
Anti-allergy; anti-atopy; lactic acid bacteria; Lactobacillus plantarum; Lactococcus lactis;
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