Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
권호 표지

Cell Co-culture Method by Patterned Gratt of Thermo-Responsive Polymer

온도응답성 고분자의 패턴상 그래프트를 이용한 공배양법

  • Bae Jin Suk (Department of Polymer Science and Engineering, Kumoh National Institute of Technology) ;
  • Ahn Chang Hyun (Department of Polymer Science and Engineering, Kumoh National Institute of Technology) ;
  • Yoon Kwan Han (Department of Polymer Science and Engineering, Kumoh National Institute of Technology) ;
  • Kwon Oh Hyeong (Department of Polymer Science and Engineering, Kumoh National Institute of Technology) ;
  • Kang Inn-Kyu (Department of Polymer Science, Kyungpook National University) ;
  • Yamato Masayuki (Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University) ;
  • Kikuchi Akihiko (Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University) ;
  • Okano Teruo (Institute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University)
  • 배진숙 (금오공과대학교 고분자공학과) ;
  • 안창현 (금오공과대학교 고분자공학과) ;
  • 윤관한 (금오공과대학교 고분자공학과) ;
  • 권오형 (금오공과대학교 고분자공학과) ;
  • 강인규 (경북대학교 고분자공학과) ;
  • ;
  • ;
  • Published : 2005.05.01
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Abstract

Thermo-responsive poly(N-isopropylacrylamide) (PIPAAm) was covalently patterned by masked el electron beam irradiation. Introduction of PIPAAm on tissue culture polystyrene dish was confirmed by ATR-FTIR and ESCA measurements. Hepatocytes were cultured at $37^{circ}C$ on these surfaces. Cells adhered on PIPAAm-grafted domains were detached by reducing culture temperature to $20^{circ}C$. Endothelial cells were then seeded and cultured on the same surfaces. Seeded endothelial cells were selectively attached on hepatocytes detached and PIPAAm-grafted domains and could be co-cultured with hepatocytes on the same culture dishes with clear pattern. This co-culture method enabled long-term co-culture of hepatocytes with endothelial cells.

온도응답성 고분자인 PIPAAm을 포토마스크를 사용하여 전자빔조사에 의해 패턴상으로 세포배양용 폴리스티렌 접시표면에 그래프트하였다. 폴리스티렌 표면에의 PIPAAm의 그래프트는 AIR-FTIR과 ESCA에 의한 표면분석을 통해 확인하였다. 이러한 표면에 간실질세포를 $37^{circ}C$에서 배양하였고, 균일하게 간세포가 배양된 배양접시를 PIPAAm의 LCST 이하인 $20^{circ}C$로 배양온도를 낮추어 PIPAAm이 그래프트된 도메인에 접착된 간실질세포를 탈착시키고 배양접시를 다시 $37^{circ}C$로 올린 후 두 번째 세포인 혈관내피세포를 파종하여 PIPAAm이 그래프트된 도메인에만 선택적으로 접착시킴으로써 같은 평면상에서 간실질세포와 혈관내피세포를 공배양할 수 있게 되었다. 이러한 방법으로 생체외에서 간실질세포와 혈관내피세포를 장기간에 걸쳐 공배양할 수 있었다.

Keywords

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