• The Plant Pathology Journal
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• 제36권4호
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• pp.305-313
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• 2020

Host shifting and host expansion of fungal plant pathogens increases the rate of emergence of new pathogens and the incidence of disease in various crops, which threaten global food security. Magnaporthe species cause serious disease in rice, namely rice blast disease, as well as in many alternative hosts, including wheat, barley, and millet. A severe outbreak of wheat blast due to Magnaporthe oryzae occurred recently in Bangladesh, after the fungus was introduced from South America, causing great loss of yield. This outbreak of wheat blast is of growing concern, because it might spread to adjacent wheat-producing areas. Therefore, it is important to understand the host range and population structure of M. oryzae and related species for determining the evolutionary relationships among Magnaporthe species and for managing blast disease in the field. Here, we collected isolates of M. oryzae and related species from various Poaceae species, including crops and weeds surrounding rice fields, in Korea and determined their phylogenetic relationships and host species specificity. Internal transcribed spacer-mediated phylogenetic analysis revealed that M. oryzae and related species are classified into four groups primarily including isolates from rice, crabgrass, millet and tall fescue. Based on pathogenicity assays, M. oryzae and related species can infect different Poaceae hosts and move among hosts, suggesting the potential for host shifting and host expansion in nature. These results provide important information on the diversification of M. oryzae and related species with a broad range of Poaceae as hosts in crop fields.

• 한국지역사회생활과학회지
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• 제19권4호
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• pp.659-672
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• 2008

There is a need to develop diverse cultivation species with reference to the favorites and farming experiences of allotment garden participants, in addition to the crops being cultivated in the gardens. In this study, therefore, a survey on the status of cultivation and the demand for herb crops for the garden participants was conducted. Current trend of the programmes related to allotment garden for city dwellers, executed by various relevant organizations was analyzed. A questionnaire was accomplished by adult participants experiencing farming in allotments. Likewise, interview with farm proprietors was conducted. The results showed that the number of crop species cultivated in allotments was about ten species. The purchase of seeds and nursery plants was mainly dependent on the farmland proprietors. The selection of the crops was made by both wife and husband. The status of herb utilization indicated that 5.7% of participants were cultivating or had cultivated herb plants. Sixty-two percent of participants were not cultivating herb plants because they did not know how. The purposes of using herbs were for medicine and beauty (22.0%), for spices (22.0%) and for enjoyment (29.0%). Thirty-four percent (34.0%) of participants were interested or would like to use the herbs in the future for medicine and beauty, 39.0% for spices and 31.7% for enjoyment. It is expected that the demand for herbs would increase. Also, 78.3% of participants had the intension or had taken into consideration to cultivate the herb plants, if the farmland proprietors would provide the plants. And, they had interest in planting, management and post harvest utilization and if a reference material would be provided as well. An approach to plant new crops might be possible to those who have more than four years of farming experience and possess relatively high level of cultivation techniques. In conclusion, the favorable crop cultivation strategy corresponding to the participants' abilities; and rental process were essential for the sustainable operation of allotment gardens and expanded number of participants. Moreover, there is a need to pay attention to the city dwellers who have a lot of farming experiences in allotment gardens to maintain agriculture and rural community. This is so, because they are interested in agriculture and rural community and most of them replied that they had intentions to move to rural areas in the future.

• Mycobiology
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• 제49권1호
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• pp.1-14
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• 2021

Pleurotus species are commercially essential mushrooms and widely cultivated throughout the world. The production of Pleurotus mushrooms alone accounts for around 25% of that total cultivated mushrooms globally. In America and Europe, Pleurotus species are considered specialty mushrooms, whereas, in Korea, their cultivation is economically profitable, and it is one of the highly consumed species. Pleurotus species are predominantly found in tropical forests and often grow on fallen branches, dead and decaying tree stumps, and wet logs. Biographical studies have shown that the Pleurotus genus is among the more conspicuous fungi that induce wood decay in terrestrial ecosystems worldwide due to its formidable lignin-modifying enzymes, including laccase and versatile peroxidases. Pleurotus species can be grown easily due to their fast colonization nature on diversified agro-substrates and their biological efficiency 100%. Pleurotus mushrooms are rich in proteins, dietary fiber, essential amino acids, carbohydrates, water-soluble vitamins, and minerals. These mushrooms are abundant in functional bioactive molecules, though to influence health. Pleurotus mushrooms are finding unique applications as flavoring, aroma, and excellent preservation quality. Apart from its unique applications, Pleurotus mushrooms have a unique status delicacy with high nutritional and medicinal values. The present review provides an insight into the cultivation of Pleurotus spp. using different agro-waste as growth substances paying attention to their effects on the growth and chemical composition.

• Journal of Ecology and Environment
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• 제29권3호
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• pp.227-235
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• 2006

강원도 평창군 일대에서 화전 후 묵밭의 식생 천이에 따른 식생 구조의 발달 과정과 종다양성의 변화를 조사하였다. 방치 후 경과 년 수에 따라 최상층부의 키는 초기에 빠르게 증가하고 후기에 안정화하는 경향을 나타내었다. 10, 20, 50 및 80 년차 묵밭에서 최상층부 식생의 키는 각각 4, 8, 18 및 18 m이었다. 교목층, 아교목층, 관목층 및 초본층의 4층 구조를 갖춘 완전한 형태의 숲으로 발달하기까지는 방치 후 약 35년이 경과해야 하는 것으로 추정되었다. 방치 후 경과 년 수에 따라 식생치 Ivc는 로그 함수적으로 그리고 식생치 Hcl은 직선적으로 증가하는 경향이 있었다. 묵밭의 식생천이에서 종다양성은 천이 중기인 $10{\sim}20$년차에서 최고로 증가한 다음 이후 점차 감소하는 경향을 나타내었다. 본 조사지에서 종다양성은 균등도보다 종풍부도와 매우 밀접한 상관을 나타내었다. 대부분의 목본은 개망초-쑥 단계인 $2{\sim}6$년차에 정착이 이루어져 시간 경과에 따라 이들의 우점도가 변화함으로써 Egler (1954)의 초기 종조성 모델을 따르는 것으로 확인되었다.

• 한국식생활문화학회지
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• 제35권6호
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• pp.590-596
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• 2020

Buckwheat (Fagopyrum species) has long been an excellent functional food. Besides, buckwheat sprouts contain various functional substances. In this study, we investigated the antioxidant activity of buckwheat sprouts in the context of cultivars harvested after different cultivation periods (0, 3, 5, 7, 9, 13, and 15 days after planting). Buckwheat sprouts were cultivated at 25℃ for up to 15 days and then extracted with ethanol. Antioxidant components were then extracted from sprouts and leaves using a freeze dryer. The total polyphenolic content, flavonoid content, and antioxidant activity were then analyzed. The total polyphenol content increased from 32.26 mg GA eq/100 g for raw buckwheat to 114.75 mg GA eq/100 g after 7 days of cultivation. Also, the flavonoid content increased from 20.61 mg catechin eq/100 g (0 days) to 56.54 mg/g after 9 days of cultivation. The DPPH radical scavenging activity (concentration of extract at 0.25 mg/mL) increased from 7.89% at day 0 to 53.48% after 9 days of cultivation. Additionally, the ABTS radical scavenging activity increased from 10.26% at day 0 to 32.89% after 7 days of cultivation; of note, the activity decreased afterward. These results suggest that the best buckwheat sprouts with higher biological activities are those cultivated for 7-9 days. For a complete understanding of the potential of buckwheat sprouts as functional foods, we plan to further analyze their antioxidant activity in the future.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 춘계학술대회 및 임시총회
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• pp.13-13
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• 2014

Two kinds of mushrooms, Gumji (金芝; Ganoderma) and Soji, were described in old book of Samguksagi (History of the three kingdoms; B.C 57~A.D 668; written by Bu Sik Kim in 1145) in Korea-dynasty. Many kinds of mushrooms were also described in more than 17 kinds of old books during Chosun-dynasty (1392~1910) in Korea. Nowadays, mushroom cultivation has been increased through out the world last decade years. Production of mushrooms has also been increased 10-20% and many varieties have been cultivated. Similar trends were also observed in Korea. Approximately two hundred commercial strains of 37 species in mushrooms were developed and distributed to cultivators. Somatic hybrid variety of oyster mushroom 'Wonhyeong-neutari' were developed by protoplast fusion, and distributed to grower in 1989. The fruiting body yield index of somatic hybrids of Pleurotus ranged between 27 and 155 compared to parental values of 100 and 138. In addition, more diverse mushroom varieties such as Phellinus baumi, Auricularia spp., Pleurotus ferulae, Hericium erinaceus, Hypsizigus marmoreus, Grifola frondosa, Agrocybe aegerita and Pleurotus cornucopiae have been attempted to cultivate in small scale cultivation. Production of mushrooms as food was 190,111 metric tons valued at 800 billion Korean Won (one trillion won if include mushroom factory products; 1dollar = 1,040 Won) in 2011. Major cultivated species are Pleurotus ostreatus, Pleurotus eryngii, Flammulina velutipes, Lentinula edodes, Agaricus bisporus, and Ganoderma lucidum, which cover 90% of total production. Since mushroom export was initiated from 1960 to 1980, the export and import of mushrooms have been increased in Korea. Technology developed for liquid spawn production and automatic cultivation systems lead to the reduction of the production cost resulting in the increasement of mushroom export. However some species were imported because of high production cost for these mushrooms requiring the effective cultivation methods. Developing of effective post-harvest system will be also directly related to mushroom export. In academic area, RDA scientists have been conducting mushroom genome projects. One of the main results is the whole genome sequencing of Flammulina velutipes for molecular breeding. An electrophoretic karyotype of of F. velutipes was obtained using CHEF with 7 chromosomes, with a total genome size of approximately 26.7 Mb. The mususcript of the genome of F. velutipes was published in PLOS ONE this year. For medicinal mushrooms, we have been conducting the genome research on Cordyceps and its related species for developing functional foods using this mushroom. In 2013, Korea Food and Drug Administraion (KFDA) approved Cordyceps mushroom for its value as an immune booster.

• The Plant Pathology Journal
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• 제36권4호
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• pp.346-354
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• 2020

Pectobacterium, which causes soft rot disease, is divided into 18 species based on the current classification. A total of 225 Pectobacterium strains were isolated from 10 main cultivation regions of potato (Solanum tuberosum), napa cabbage (Brassica rapa subsp. pekinensis), and radish (Raphanus sativus) in South Korea; 202 isolates (90%) were from potato, 18 from napa cabbage, and five from radish. Strains were identified using the Biolog test and phylogenetic analysis. The pathogenicity and swimming motility were tested at four different temperatures. Pectolytic activity and plant cell-wall degrading enzyme (PCWDE) activity were evaluated for six species (P. carotovorum subsp. carotovorum, Pcc; P. odoriferum, Pod; P. brasiliense, Pbr; P. versatile, Pve; P. polaris, Ppo; P. parmentieri, Ppa). Pod, Pcc, Pbr, and Pve were the most prevalent species. Although P. atrosepticum is a widespread pathogen in other countries, it was not found here. This is the first report of Ppo, Ppa, and Pve in South Korea. Pectobacterium species showed stronger activity at 28℃ and 32℃ than at 24℃, and showed weak activity at 37℃. Pectolytic activity decreased with increasing temperature. Activity of pectate lyase was not significantly affected by temperature. Activity of protease, cellulase, and polygalacturonase decreased with increasing temperature. The inability of isolated Pectobacterium to soften host tissues at 37℃ may be a consequence of decreased motility and PCWDE activity. These data suggest that future increases in temperature as a result of climate change may affect the population dynamics of Pectobacterium.

• 한국잡초학회지
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• 제13권2호
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• pp.159-163
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• 1993

건답직파(乾畓直播)를 연속(繼續)함에 따라서 야기되는 잡초군락(雜草群落)의 변이양상(變異樣相)을 밝혀 효과적(效果的)인 제초체계(除草體系)를 확립(確立)하고자 수행(遂行)한 결과(結果)는 다음과 같다. 1. 벼 건답직파재배(乾畓直播栽培)를 4년간(年間) 연속재배(連續栽培)한 경우 화본과잡초(禾本科雜草)는 감소(減少)한 반면 광엽잡초(廣葉雜草)는 증가(增加)하였으며 생활형(生活型)으로 보면 1년생잡초(年生雜章)는 증가(增加)하였으나 다년생잡조(多年生雜草)는 감소(滅少)하였다. 2. 기계이앙시(機械移秧時) 우점잡초(優占雜草)는 올방개-피-올챙이고랭이-물달개비 등의 수생잡초(水生雜草)가 우점(優占)하였으나 건답직파재배(乾畓直播栽培)를 연속(繼續)할 경우 피-바랭이-가막사리-조개풀-자귀풀 등의 습생잡초(濕生雜草)가 우점(優占)하였다. 3. 건답직파(乾畓直播)에 따른 초종(草種)의 다양화(多樣化) 정도(程度)(Simpson Index) 및 군락우점도(群落優占度)(Community dominance)를 보면 기계이앙(機械移秧)의 경우 1-2 초종(草種)이 우점화(優占化)되는 경향(傾向)이었으나 건답직파(乾畓直播)의 경우 여러 초종(草種)이 다양(多樣)하게 분포(分布)되어 있었다. 4. 직파재배(直播栽培)에 따른 연도별(年度別) 유이성(類似性) 계수(係數)(Similarity coefficient)를 보면 직파재배(直播栽培)의 첫해는 41%의 유이성(類似性) 계수(係數)를 나타내 기계이앙(機械移秧)과 상당히 달랐으나 4년간(年間) 연속직파재배(連續直播栽培)를 할 경우 67%로 높아졌다. 5. 건답직파(乾畓直播) 첫해인 1979년도(年度) 잡초방임구(雜草放任區)의 잡초(雜草) 건물중(乾物重)은 $m^2$당 61.3g에 벼 수량(收量)은 290kg/10a이었으나 3년후(年後)인 1992년(年)에는 잡초건물중(雜草乾物重)이 293g/$m^2$으로 1979년에 비해 4.7배가 증가(增加)하였으며 벼 수량(收量)은 전무(全無)였다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.131.2-132
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• 2003

White rot on garlic caused by Sclerotium cepivorum firstly occurred at Goheoung, Jeonnam in 1998. Thereafter, the disease rapidly spread throughout the country except Gangwon and became a major limiting factor for the cultivation of various Allium species such as garlic, onion, and welsh onion. The disease that has not been reported on a wild garlic(Allium monanthum) previously occurred severely at Seosan, Choongnam in 2003. Among cultivation areas in the region, 10.7％ were infected by the disease and the ratio of diseased plant reached up to 55.0％ in some heavily infected fields. Two species of Sclerotium were consistently isolated from infected samples and identified as S. cepivorum or another Sclerotium sp. Averaged size of sclerotium of the former was 455.0x562.2 urn, while the later was 374.4${\times}$347.2$\mu\textrm{m}$. Patogenicity to Allium species and mycological characteristics such as sclerotium size, growth temperature, and microconidia of the fungi were similar to those reported on other Allium species previously. Consequently, the wild garlic is a newly reported host of the two pathogenic fungi in Korea.

• Journal of Species Research
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• 제13권3호
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• pp.288-292
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• 2024

In the course of our survey of microbial diversity, a total of seven basidiomycetous yeast strains were isolated from flowers sampled across the four provinces of the Republic of Korea, of which two strains were identified as an unrecorded species. Comparative analysis was conducted for identification of strains using the D1/D2 region of the large subunit(LSU) rRNA gene sequences, observation of cell morphologies, and carbon assimilation tests. Seven strains were classified within the phylum Basidiomycota. Two unrecorded strains, JRP1-10 (KACC 410703) and KC-1 (KACC 410704), were identified as Vishniacozyma pseudocarnescens, belonging to the family Bulleribasidiaceae, order Tremellales, class Tremellomycetes. Two strains had ellipsoidal-shaped and monopolar budding cells and smooth and cream-colored colonies after cultivation at 25℃ for 3 days. Here, we present the morphological and biochemical characteristics of the unrecorded yeast species, Vishniacozyma pseudocarnescens, in the Republic of Korea.