• The Journal of Korean Physical Therapy
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• 제20권4호
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• pp.51-59
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• 2008

Purpose: The aim of this study was to evaluate the correlation between the types of leisure activity and Korean form of geriatric depression score in the elderly. Methods: We surveyed elderly (>60 years old) people who participated in leisure activities using interviews in Bukgu district, Daegu city. Data collection was performed from 1 - 31 May, 2008. The data was analyzed with SPSS 12.0 program, which was used for descriptive statistics and Pearson correlation analysis. Results: Common leisure activities scored 'often' and 'very often' were family conversations, offspring visiting, eating out, plant cultivation, entertainment, conversation, walking or jogging, and TV or radio. It was investigated at the rate of 19.2% in the moderate depression and 8.3% in the severely depression. Depression positively associated with newspaper or magazine, family conversation, offspring visiting, social service, movie & theater, exercise and tour. Conclusion: Most elderly people surveyed indicated they were depressed, which should recommend significant leisure activities for elderly to reduce depression in Bukgu district, Daegu city.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.229-236
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• 1998

6-Substituted derivatives of 1-［(2-hydroxyethoxy)methyl］-6-(phenylthio)thymine (HEPT) were synthesized by introducing alkyl groups with the aid of chlorotrimethylsilane, and then purified ranging 40 to 81 % of yield. Because of their peculiar structures, we presumed that HEPT derivatives would contain extra biological activities other than their already known anti-human immunodeficiency viral (HIV -1) activities. In this study, we investigated the possible effects of the HEPT derivatives on bacterial growth and found their selective antibiotic activities against gram-positive strains. We could not observe the corresponding activity from a disc-zone test, but confirmed the activity by liquid cultivation. Since the growth rate of cells was easily recovered, the antibiotic function was suggested to be bacteriostatic. We also suggested that the intracellular fate of HEPT derivatives would be fast. A HEPT derivative f-3 was shown to synergize unidirectionally toward chloramphenicol (Chr). With 0.1 mM f-3, the Chr-directed growth-inhibitory curve appeared 4 hours earlier than found without the additive. Interestingly, from the data of SDS-polyacrylamide gel electrophoresis (PAGE), we found that a membrane-bound protein having a molecular weight of 70-kDa was overexpressed by f-3 in S. aureus.

• Journal of Microbiology and Biotechnology
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• 제2권3호
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• pp.209-214
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• 1992

Serratia marcescens co-cultured with various phytopathogenic fungi, including Rhizopus stolonifer, Helminthosporium allii, Pyricularia oryzae, Fusarium oxysporium and Collectothricom cassiicola, in an LB- agar medium containing 1.5% swollen chitin, significantly inhibitied fungal growth. Fungal hyphae grew rapidly outward from the culture dish center, but the hyphal extensions of the pathogenic fungi were significantly inhibited in a perimetric contact area with S. marcescens. This was especially evident in pathogenic fungi which have a high chitin content in their cell walls. The extracellular chitinase activities of S. marcescens were increased seven fold by the addition of 1.5% swollen chitin to the LB-broth, compared to chitinase activities in a culture medium without chitin. The type of induction was dependent on the various forms of chitin used. When the culture supernatant of S. marcescens or the chitinases of Streptomyces griceus purchased from Sigma Chemical Co., were incubated with the mycelium of F. oxysporium, the mycelium gradually burst as cultivation time progressed and completely lysed after incubation for 2 days. On the other hand, E. coli extract did not hydrolyze the F. oxysporium mycelium at all. These data showed that the chitinolytic activities of S. marcescens play important roles in the biochemical control of phytopathogenic fungi.

• 한국식품영양학회지
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• 제30권3호
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• pp.454-463
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• 2017

Ganoderma lucidum has been traditionally used as a medicine for treatment of bronchitis, arthritis, and high blood pressure, and it has been reported to display many biological activities including anticancer and immune activities. Since mushroom mycelium is known to have excellent biological activities together with mushroom fruiting body, studies on biological activities of mushroom mycelium have been actively conducted. Thus, the present study compared the biological activities before and after the cultivation of Ganoderma lucidum mycelium on Atractylodes rhizoma. When the radical scavenging activity was assessed by the DPPH assay, ARGL (ethanol extract of Atractylodes rhizoma mycelium fermented with Ganoderma lucidum) showed radical scavenging activity of 5.58~82.56% at concentrations of $10{\sim}500{\mu}g/assay$, while AR (ethanol extract of Atractylodes rhizoma) showed radical scavenging activity of 5.27~72.08% at the same concentrations. When measured by using the ABTS assay, ARGL showed higher radical scavenging activity than AR, which was consistent with the result obtained by the DPPH assay. In the MTT assay, the cytotoxicity of ARGL against all cell lines was higher than that of AR. In particular, the cytotoxicities of AR and ARGL against Hep3B at a concentration of $400{\mu}g/assay$ were 71.81% and 86.40%, respectively. In addition, the result obtained by the SRB assay was consistent with the result obtained by the MTT assay. According to the results mentioned above, there is a high probability that medicinal herb cultures using mycelium can be used as sources of functional foods since the cytotoxicities against cancer cells and antioxidant activities increased when the mycelium was fermented with Atractylodes rhizoma.

• 한국토양비료학회지
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• 제37권2호
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• pp.109-115
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• 2004

가축분 퇴비가 토양의 생화학적 특성에 미치는 영향을 조사하고자, 농업과학기술원 시험포장에 시용량 ($8Mg\;ha^{-1}$, CM-8구: $29Mg\;ha^{-1}$, CM-29구: $57Mg\;ha^{-1}$, CM-57구)을 달리하여 돈분퇴비를 시비하고 봄배추를 재배하면서 생육시기별로 토양을 채취하여 biomass, 효소활성도의 변화를 조사하였으며, 그 결과를 요약하면 다음과 같다. 토양효소 활성은 NPK구의 경우 대조구에 비하여 낮았으나 퇴비구에서는 증가하여 CM-57구에서 protease, Phosphomonoesterase 그리고 dehydrogenase의 활성이 각각 2.3, 1.6 및 2.4배 증가하였다. Protease와 phosphomonoesterase의 경우 재배기간중 모든 처리구에서 생육중기에 가장 높은 활성을 나타내었다. Biomass는 퇴비의 시용랑에 따라 증가하여 biomass C의 함량은 CM-57구에서 $466mg\;kg^{-1}$으로 대조구에 비하여 4.3배, biomass N과 P는 각각 3.4배, 2.8배 증가하였다. 한편 NPK구에서는 biomass C와 N은 증가하였으나 biomass P는 낮았다. 퇴비구에 있어서 biomass N과 P는 배추의 생육중기에 최고치에 달하였으나 biomass C의 함량은 생육초기에 증가하여 수확기까지도 높은 함량을 유지하였다. 평균 biomass C:N:P 비율은 11:2:1이었으며, 토양의 유기 탄소 및 질소함량에 대한 biomass C와 N의 비율은 각각 1.1 및 3.6%이었다. 재배토양의 protease와 dehydrogenase는 biomass C 및 P와 고도로 유의한 상관관계를 나타내었다.

• 생명과학회지
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• 제32권2호
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• pp.155-160
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• 2022

세계 4대 섬유작물중의 하나로 알려진 케나프(Hibiscus cannabinus L.)는 탄소 저감용 및 친환경 생활소재용으로 각광받고 있으며, 2013년 국내 채종 가능한 '장대' 품종이 확립되면서 전국적으로 재배되고 있다. 그러나, 케나프의 부위별 생리활성, 특히 '장대'의 부위별 생리활성 평가는 미미한 실정이다. 본 연구에서는 케나프 '장대'의 종실(seed), 종자 꼬투리(seedpod), 잎(leaf), 줄기(stem), 뿌리(root) 에탄올 추출물을 조제하여 이의 항혈전 및 항산화 활성을 평가하였다. 그 결과 종실 추출물에서 강력한 혈액응고인자 저해활성을, 종자 꼬투리 추출물에서 프로트롬빈 저해 활성을, 줄기 추출물에서 트롬빈 저해활성을 확인하였다. 또한 종자 꼬투리 추출물에서는 항혈전 활성과 연관된 DPPH 음이온 소거능, ABTS 양이온 소거능, 환원력 및 nitrite 소거능이 여타의 부위 추출물보다 유의적으로 강력한 활성을 나타냄을 확인하였다. 본 연구결과는 국내 케나프 친환경 섬유산업의 부산물로 얻어지는 종자 꼬투리, 뿌리 및 줄기를 이용한 고부가가치 생물소재 개발이 가능함을 제시하고 있다.

• 한국토양비료학회지
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• 제45권1호
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• pp.25-29
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• 2012

Agriculture activities account for 58% of total anthropogenic emissions of nitrous oxide ($N_2O$) with global warming potential of 298 times as compared to carbon dioxide ($CO_2$) on molecule to molecule basis. Quantifying $N_2O$ from managed soil is essential to develop national inventories of greenhouse gas (GHG) emissions. The objective of the study was to compare $N_2O$ emission from livestock compost applied arable land with that for fertilizer treatment. The study was conducted for two years by cultivating Chinese cabbage (Brassica campestris L.) in Chuncheon, Gangwon-do. Accumulated $N_2O$ emission during cultivation of Chinese cabbage after applying livestock compost was slightly greater than that for chemical fertilizer. Slightly greater $N_2O$ emission factor for livestock compost was observed than that for chemical fertilizer possibly due to lump application of livestock compost before crop cultivation compared with split application of chemical fertilizers and enhanced denitrification activity through increased carbon availability by organic matter in livestock compost.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.232-237
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• 2005

The cellulloytic enzymes, such as FPase and amylase, production by Trichoderma inhamatum KSJ1 using 10L air circulation bioreactor were tried. Physical properties of the employed bioreactor, mixing time, $k_{L}$ a were examined in airflow conditions of enzyme production cultures. Mixing time of distilled water at 0.6, 1.2, 1.8 vvm were 25, 21, 9 sec, respectively, and mandel's medium using rice straw and pulp as carbon sources were 31, 25, 15 sec. Mixing time decreased when airflow rate increased. $k_L$ a of distilled water at 0.6, 1.2, 1.8 vvm were measured -22.52, -29.31, -39.62, respectively, and the mandel's medium were -47.8, -18.5, -21.4. The $k_L$ a values was increased linearly as the air-flow rates increased. However the values in mandel's medium showed different results, especially at 0.6 vvm. Amylase and FPase activity at the 1.2 vvm was 3.39U/mL, 0.72U/mL in 5 days cultivation. and the activities at 0.6 vvm were 2.01U/mL, 0.39U/mL, respectively. The enzyme productivity at 1.2 vvm was higher than at 0.6 vvm, indicating that high oxygen tension is requested for the efficient enzyme production culture of T. inhamatum KSJ1.

• 한국자원식물학회지
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• 제24권3호
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• pp.330-336
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• 2011

The recent disaster of earthquake and tsunami on March 11, 2011 severely attacked East Japanese cities and people, and in addition, the accident of nuclear power station will inevitably damage the agricultural activities there. The economical depression influences on the horticulture and floriculture industry as well, through the reluctant consumption all over the country. Such a situation reflects a conventional perception that the garden plants or ornamental plants have been regarded as a symbol of capitalism, representing the success, luxury, beauty or other metaphors indicating the winners of business war. But as the word "culture" means "cultivation" originally, horti "culture" or flori "culture" should have played some roles in cultivating lands as well as cultivation of human minds, leading to develop a modern "culture" which may lay emphases on personality, originality, partnership, cooperation, diversity and so forth. In this article, a brief history of garden plants in Japan, as well as some current movements in Japanese horticulture and floriculture, is reviewed with some commodities which possess messages on creating a new humane culture.

• 한국미생물·생명공학회지
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• 제18권4호
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• pp.383-389
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• 1990

Penicillium verruculosum 섬유소 분해효소의 유도물질인 KC-flock 및 cellobiose octaacetate(COA)를 유일한 탄소원으로 포함하는 배지에서 21일간 배양하면서 배양여액 중의 섬유소 분해효소 각 성분의 활성도와 전기영동상의 단백질 양상을 검토하였다. 배양여액의 중의 총단백질 생성 및 섬유소 분해활성의 유도 효과는 COA 배지가 다른 두 배지의 3배 이상이었다. 배양기간의 증가에 따라 배양여액 중의 총단백질량이 증가되었는데 CMC 분해활성이나 $\beta$-glucosidase 활성의 증가보다는 avicel 분해활성도의 증가가 총단백질량의 증가와 유사하다.