• Title/Summary/Keyword: cucumis melo

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Biological and Molecular Characterization of a Korean Isolate of Cucurbit aphidborne yellows virus Infecting Cucumis Species in Korea

  • Choi, Seung-Kook;Yoon, Ju-Yeon;Choi, Gug-Seoun
    • The Plant Pathology Journal
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    • v.31 no.4
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    • pp.371-378
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    • 2015
  • Surveys of yellowing viruses in plastic tunnels and in open field crops of melon (Cucumis melo cultivar catalupo), oriental melon (C. melo cultivar oriental melon), and cucumber (C. sativus) were carried out in two melon-growing areas in 2014, Korea. Severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. The symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thickening of older leaves. RT-PCR analysis using total RNA extracted from diseased leaves did not show any synthesized products for four cucurbit-infecting viruses; Beet pseudo-yellows virus, Cucumber mosaic virus, Cucurbit yellows stunting disorder virus, and Melon necrotic spot virus. Virus identification using RT-PCR showed Cucurbit aphid-borne yellows Virus (CABYV) was largely distributed in melon, oriental melon and cucumber. This result was verified by aphid (Aphis gossypii) transmission of CABYV. The complete coat protein (CP) gene amplified from melon was cloned and sequenced. The CP gene nucleotide and the deduced amino acid sequence comparisons as well as phylogenetic tree analysis of CABYV CPs showed that the CABYV isolates were undivided into subgroups. Although the low incidence of CABYV in infections to cucurbit crops in this survey, CABYV may become an important treat for cucurbit crops in many different regions in Korea, suggesting that CABYV should be taken into account in disease control of cucurbit crops in Korea.

The Effects of CaCl2 Foliar Application on Inhibition of Abnormally Fermented Fruits and Chemical Composition of Oriental Melon(Cucumis melo L. var. makuwa Mak.) (CaCl2의 엽면처리(葉面處理)가 참외의 이상발효과 발생억제 및 과실성분(果實成分)에 미치는 영향)

  • Chung, Hee-Don;Youn, Sun-Joo;Choi, Young-Jun
    • Horticultural Science & Technology
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    • v.16 no.2
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    • pp.215-218
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    • 1998
  • The effects of $CaCl_2$ foliar application on inhibition of the occurrence of abnormally fermented fruit and chemical composition of oriental melon (Cucumis melo L. var. makuwa Mak. cv. Gumssaragi-eunchun) were examined. $CaCl_2$, applied at 0.3~0.7%, had an apparent inhibitory effect on occurrence of abnormally fermented fruit when sprayed three times at five days intervals from 10 days after flowering. The effect of $CaCl_2$ was diminished when the field was irrigated during the ripening period. Sugar content in the fruits was not affected by the $CaCl_2$ treatment. The Ca content was higher in the normal fruit than in the fermented ones, but no difference was noted in the K and Mg levels. The occurrence of fermented fruit was increased when the applying nitrogen levels were less (10kg) or higher (25kg/10a) than normal supply. The nitrogen application had no effect on the calcium content in fruits.

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Marker-Assisted Selection for Monoecy in Chamoe (Cucumis melo L.) (성발현 연관 분자마커를 이용한 단성화 참외 선발)

  • Bang, Sun-Woong;Song, Kihwan;Sim, Sung Chur;Chung, Sang Min
    • Horticultural Science & Technology
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    • v.34 no.1
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    • pp.134-141
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    • 2016
  • The DNA marker T1ex, originally developed from melon (Cucumis melo L.) for monoecy, was employed in chamoe, which is referred to as oriental melon. This marker shows size variations in monoecious melon. However, in chamoe, no such detrimental size variation was found in monoecious chamoe, and 99% association between flower phenotypes and genotypes of the T1ex marker was observed in 106 lines of chamoe. To evaluate the efficacy of the T1ex marker for marker-assisted selection (MAS), a total of 240 plants of chamoe breeding lines were screened using the T1ex marker. Among these, 98 varieties were selected. Although the T1ex marker might not be useful for MAS in melon, we found 100% concordance between genotypes and phenotypes for sex expression in chamoe. These results suggest that the T1ex marker will be a useful resource for MAS for monoecy in chamoe.

Effect of Mulching Material on Growth and Yield of Cucumis melo L. cv. Reticulatus (멀칭재료가 멜론 생육 및 수량에 미치는 영향)

  • Kyu Hoi Lee;Min-Jeong Lee;Ju Hyoung Kim;Young Ho Kim
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.67-67
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    • 2020
  • 멜론(Cucumis melo L. cv. Reticulatus)은 다른 과채류에 비해 재배기간이 짧고 수확 시 노동력이 덜 필요로 하는 작물로 고품질 멜론 생산 시 판로 및 가격이 높게 형성되어 농가소득 향상에 적합한 작물이다. 멜론의 국내 시장규모는 1,000억원 정도이며, 일본의 멜론 시장은 4조원으로 40배 정도 큰 시장을 형성하고 있으므로, 수출을 위한 고품질 재배기술 개발이 필요하다. 따라서, 본 연구는 충청북도농업기술원 시험연구포장에서 고온기 근권 환경 최적화를 위한 멀칭 방법을 구명하기 위해 수행하였다. 멜론은 얼스마운틴 PMR 품종을 4월 29일에 120 × 50 cm 로 정식하였으며, 멀칭 처리는 녹색비닐, 검정부직포, 종이멀칭지를 난괴법 3반복으로 각각 피복하였다. 멀칭자재별 생육특성 중 초장은 녹색비닐과 종이멀칭 피복처리에서 각각 148.1 cm, 144.8cm 로 우수하였으며, 엽폭 및 엽장은 유의성 있는 차이가 나타나지 않았다. 생체중은 종이멀칭지로 피복한 처리에서 716.7 g plant-1 로 가장 우수하게 나타났다. 과특성 중 과직경은 녹색비닐과 종이멀칭 피복처리에서 각각 16.1 cm로 우수하였으며, 과중은 종이멀칭지로 피복한 처리에서 2,235 g plant-1 로 가장 우수하게 나타났다. 이처럼 멀칭재료에 따른 생육특성 차이를 확인하였으며, 이러한 적정 멀칭자재 선발을 통해 여름철 고온기 근권 환경 최적화를 통한 멜론 수량 및 품질향상에 기여할 것으로 판단된다.

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Growth and Quality of Muskmelon (Cucumis melo L.) as Affected by Fruiting Node Order, Pinching Node Order and Harvest Time in Hydroponics Using Coir Substrate (코이어 배지를 이용한 멜론(Cucumis melo L.) 수경재배 시 착과 절위, 적심 절위 및 과실 수확시기에 따른 멜론의 생육 및 품질 특성)

  • Lim, Mi Young;Choi, Su Hyun;Choi, Gyeong Lee;Kim, So Hui;Jeong, Ho Jeong
    • Journal of Bio-Environment Control
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    • v.29 no.4
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    • pp.406-413
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    • 2020
  • This study was conducted to find out optimum fruiting node order, pinching node order, and harvesting time in hydroponics using coir substrates to produce high quality melon (Cucumis melo L.) fruit. Three plants per coir slab (100 × 20 × 10 cm) were planted for each treatment. Yamazaki standard nutrient solutions for melon were supplied with 1.8, 2.0, and 2.3 dS·m-1 at the early, middle (fruit enlargement step), and late growth stages, respectively. Two cultivars of 'PMR Dalgona' and 'Earl's Aibi' were used for fruiting node order and pinching node order experiments. Fruiting node treatments were conducted three replications (8-10 th, 11-13 th, and 14-15 th nodes) and pinching node treatments treated with three replications (18 th, 21 th, and 24 th nodes). Two cultivars of 'PMR Dalgona' and 'Earl's Crown' were used for fruit harvesting time experiment and treated with in four replications (45, 50, 55, and 60 days after fruiting). In growth characteristics, the leaf width and leaf area of 'PMR Dalgona' were the greatest 28.2 cm and 10,845 ㎠. Respectively, 11-13 th fruiting nodes or more. The node length of 'Earl's Aibi' was the longest by 147.6 cm at 11-13 th fruiting nodes. For fruit quality characteristics, the fruit weight of 'Earl's Aibi' at 11-13 th fruiting node fruiting was the greatest by 2.0 kg. The soluble solids content (SSC) of 'PMR Dalgona' was the highest by 14.5 °Brix at 8-10 th nodes in fruiting node orders and 14.5 °Brix at the 24 th pinching node order, respectively with significant difference. The SSC tends to increase in the same for both cultivars of 'PMR Dalgona' and 'Earl's Aibi' as the position of fruiting node was lower. The SSC and fruit weight of melon harvested at 55-60 days after fruiting was the best. From the results of this study, most of SSC tends to increase in the lower position of fruiting node order and the higher pinching node order, whereas the fruit weight shows a tendency of increasing with higher fruiting node. In addition, the SSC of fruit increased as the number of days after fruiting increased, and further research is needed for more various cultivars. In melon hydroponics using coir substrates, it is needed to figure out the characteristics of each cultivar to determine optimum fruiting node order, pinching node order, and fruit harvest time.

Expression Profiling of MLO Family Genes under Podosphaera xanthii Infection and Exogenous Application of Phytohormones in Cucumis melo L. (멜론 흰가루병균 및 식물 호르몬 처리하에서 MLO 유전자군의 발현검정)

  • Howlader, Jewel;Kim, Hoy-Taek;Park, Jong-In;Ahmed, Nasar Uddin;Robin, Arif Hasan Khan;Jung, Hee-Jeong;Nou, III-Sup
    • Journal of Life Science
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    • v.26 no.4
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    • pp.419-430
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    • 2016
  • Powdery mildew disease caused by Podosphaera xanthii is a major concern for Cucumis melo production worldwide. Knowledge on genetic behavior of the related genes and their modulating phytohormones often offer the most efficient approach to develop resistance against different diseases. Mildew Resistance Locus O (MLO) genes encode proteins with seven transmembrane domains that have significant function in plant resistance to powdery mildew fungus. We collected 14 MLO genes from ‘Melonomics’ database. Multiple sequence analysis of MLO proteins revealed the existence of both evolutionary conserved cysteine and proline residues. Moreover, natural genetic variation in conserved amino acids and their replacement by other amino acids are also observed. Real-time quantitative PCR expression analysis was conducted for the leaf samples of P. xanthii infected and phyto-hormones (methyl jasmonate and salicylic acid) treated plants in melon ‘SCNU1154’ line. Upon P. xanthii infection using 7 different races, the melon line showed variable disease reactions with respect to spread of infection symptoms and disease severity. Three out of 14 CmMLO genes were up-regulated and 7 were down-regulated in leaf samples in response to all races. The up- or down-regulation of the other 4 CmMLO genes was race-specific. The expression of 14 CmMLO genes under methyl jasmonate and salicylic acid application was also variable. Eleven CmMLO genes were up-regulated under salicylic acid treatment, and 7 were up-regulated under methyl jasmonate treatments in C. melo L. Taken together, these stress-responsive CmMLO genes might be useful resources for the development of powdery mildew disease resistant C. melo L.

Host Plant and Damage Symptom of Fungus Gnats, Bradysia spp. (Diptera: Sciaridae) in Korea (Fungus gnats, Bradysia spp.의 기주 및 피해증상)

  • 이흥수;김태성;신현열;김형환;김규진
    • Korean journal of applied entomology
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    • v.40 no.2
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    • pp.149-153
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    • 2001
  • We surveyed on the host plants of Fungus gnat, Bradysia app. and found 21 species in the greenhouse and field. These are as follows: Cucumis sativus L., Cucumis melo L., Citrullus lanatus T., Cucurbita moschata F., Lycopersicon esculentum M., Capsicum annuum L (Pepper), Capsicum annuum L (Paprika), Lillium longiflorum T., Dianthus caryophyllus L., Rosa hybrida H., Gerbera jamesonii B., Chrysanthemum morifolium R, Phalaenopsis schilleriane R., Gladiolus grandiflours H., Zingiber officinale R., Cnidium officinale M., Canavalia gladiata DC., Angelica utilis M., Polygonatum odoratum D., Pinus densiflora S., and Pinus thunbergii P. Fungus gnat larvae cause damages to the root and promote decay and wilt by feeding on the roots and burrowing in plant tissue.

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Pathogenicity of Didymella bryoniae on the Seedlings of Cucurbits (오이류 유묘에 대한 덩굴마름병균의 병원성)

  • Lee Du Hyung
    • Korean Journal Plant Pathology
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    • v.1 no.3
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    • pp.173-177
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    • 1985
  • The objective of the study is to determine differences between cucurbits in the pathogenicity of Didymella bryoniae isolated from the naturally infected seeds of cucumber and pumpkin. Primary seedling infection of cucumber(Cucumis sativus L.), oriental melon(Cucumis melo var. makuwa Makino), pumpkin(Cucurbita pepo L.) and watermelon (Citrullus vulgaris Shrad.) occurred on the radicle, hypocotyl and cotyledons and symptoms on each crop were very similar. Infection of the radicle generally caused pre-emergence rot, while infection on the hypocotyl and cotyledons provided further inoculum for infection of the first true leaves and the stem. In cross inoculation tests, all isolates of D. bryoniae could infect cucumber, oriental melon, pumpkin and watermelon at different growth stages and there were not much differences in pathogenicity or susceptibility between isolates of the pathogen and crops tested. The susceptibility of cucumber and pumpkin was markedly influenced by prevailing humid conditions.

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Occurrence of Root-knot Nematodes on Fruit Vegetables Under Greenhouse Conditions in Korea (과채류 시설재배지의 뿌리혹선충 문제)

  • 김동근
    • Research in Plant Disease
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    • v.7 no.2
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    • pp.69-79
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    • 2001
  • Meloidogyne arenaria race 2 (59%) is widely distributed, followed by M. incognita race 1 (23%), and an unknown race of M. incognita (18 %) in greenhouses in southern Korea. The key character to distinguish between M. arenaria and M. incognita is excretory pore in female head. When oriental melon, Cucumis melo L., grafted on Shintozoa (Cucurbit maxima x Cu. moschata) is transplanted in February in a plastic tunnel inside a greenhouse infested with M. arenaria, nematodes produced egg masses on roots at 40 days after transplanting and the soil juveniles (J2) population reach maximum in July to 3,817/100 ㎤. Juveniles are distributed relatively uniform over the 180-cm-wide row horizontally and the highest density occurs at 0-25 cm soil depth. For the control of root-knot nematodes, rice rotation, solarization, and soil addition treatments are the most effective (P=0.05); treatments reduce number of J2 over 90% and increase yield two times. Corn retation, fosthiazate, and soil drying treatment are moderately effective, while sesame and green onion rotations are not effective. The relationship between M. arenaria and yield of oriental melon is adequately described by a linear regression model. In the test with wild Cucumis genetic sources introduced from U.S.Dept. of Agriculture (USDA), one of C.heptadactylus, two of C.anguria, two of C. anguria var. longaculeatus, nine of C. metuliferus are resistant to both species of root-knot nematodes.

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Inhibition of Preharvest Drops by AVG Foliar Application in Staking Cultivation of Oriental Melon (Cucumis melo L. var. makuwa Mak.) Plants (AVG 처리(處理)에 의한 지주재배(支柱栽培) 참외의 낙과억제(落果抑制))

  • Chung, Hee-Don;Choi, Young-Jun;Ju, Sung-Don
    • Horticultural Science & Technology
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    • v.18 no.6
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    • pp.792-796
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    • 2000
  • The staking cultivation (SC) of oriental melon (Cucumis mela L. var. makuwa Mak.) produced more fruit yield than that of the conventional (creeping) growing. In the SC, preharvest fruit drop rate was high as 47.6% compared to 13.0% of the conventional cultivation. However, the foliar application of aminoethoxyvinylglycine (AVG, $100mg{\cdot}L^{-1}$) at 2 weeks after flowering (20 days before harvest) greatly reduced fruit drops up to 10.9% without causing any effects on ripening and quality of fruits . The AVG application markedly suppressed ethylene production in harvested fruits.

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