• 제목/요약/키워드: crystal orientation

검색결과 569건 처리시간 0.026초

Use of Molecular Replacement to Determine the Phases of Crystal Structure of Taq DNA Polymerase

  • Kim, Young-Soo;Suh, Se-Won
    • BMB Reports
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    • 제29권1호
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    • pp.38-44
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    • 1996
  • Taq DNA polymerase from Thermus aquaticus has been shown to be very useful in the polymerase chain reaction method, which is being used for amplifying DNA. Not only does Taq DNA polymerase have high commercial value commercial value for the polymerase chain reaction application, but it is also important in studying DNA replication, because it is apparently an homologue to E. coli DNA polymerase I, which has long been used for DNA replication study (Lawyer et ai., 1993). The crystal structure determination of Taq DNA polymerase was initiated. An X-ray diffraction pattern breaks down a crystal structure into discrete sine waves in a Fourier series. The original shape of a crystal object in terms of electron density may be represented as the sum of those sine waves with varying amplitudes and phases in three dimensions. The molecular replacement method was initially employed to provide phase information for the structure of Taq DNA polymerase. The rotation search using the program MERLOT resulted in a solution peak with 5.4 r.m.s. PC-refinement of the X-PLOR program verified the result and also optimized the orientation angles. Next, the translation search using the X-PLOR program resulted in a unique solution peak with 7.35 r.m.s. In addition, the translation search indicated $P3_121$ to be the true space group out of two possible ones. The phase information from the molecular replacement was useful in the MIR phasing experiment.

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Transverse Wave Propagation in [ab0] Direction of Silicon Single Crystal

  • Yun, Sangjin;Kim, Hye-Jeong;Kwon, Seho;Kim, Young H.
    • 비파괴검사학회지
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    • 제35권6호
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    • pp.381-388
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    • 2015
  • The speed and oscillation directions of elastic waves propagating in the [ab0] direction of a silicon single crystal were obtained by solving Christoffel's equation. It was found that the quasi waves propagate in the off-principal axis, and hence, the directions of the phase and group velocities are not the same. The maximum deviation of the two directions was $7.2^{\circ}$. Two modes of the pure transverse waves propagate in the [110] direction with different speeds, and hence, two peaks were observed in the pulse echo signal. The amplitude ratio of the two peaks was dependent on the initial oscillating direction of the incident wave. The pure and quasi-transverse waves propagate in the [210] direction, and the oscillation directions of these waves are perpendicular to each other. The skewing angle of the quasi wave was calculated as $7.14^{\circ}$, and it was measured as $9.76^{\circ}$. The amplitude decomposition in the [210] direction was similar to that in the [110] direction, since the oscillation directions of these waves are perpendicular to each other. These results offer useful information in measuring the crystal orientation of the silicon single crystal.

속도의존성 결정소성 모델 기반의 유한요소해석을 통한 BCC 금속의 변형 집합조직 예측 (Prediction of Deformation Texture in BCC Metals based on Rate-dependent Crystal Plasticity Finite Element Analysis)

  • 김동규;김재민;박원웅;임용택;이용신
    • 소성∙가공
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    • 제23권4호
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    • pp.231-237
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    • 2014
  • In the current study, a rate-dependent crystal plasticity finite element method (CPFEM) was used to simulate flow stress behavior and texture evolution of a body-centered cubic (BCC) crystalline material during plastic deformation at room temperature. To account for crystallographic slip and rotation, a rate-dependent crystal constitutive law with a hardening model was incorporated into an in-house finite element program, CAMPform3D. Microstructural heterogeneity and anisotropy were handled by assigning a crystallographic orientation to each integration point of the element and determining the stiffness matrix of the individual crystal. Uniaxial tensile tests of single crystals with different crystallographic orientations were simulated to determine the material parameters in the hardening model. The texture evolution during four different deformation modes - uniaxial tension, uniaxial compression, channel die compression, and simple shear deformation - was investigated based on the comparison with experimental data available in the literature.

Luminescent Properties of BaSi2O5:Eu2+ Phosphor Film Fabricated by Spin-Coating of Ba-Eu Precursor on SiO2 Glass

  • Park, Je Hong;Kim, Jong Su;Kim, Jong Tae
    • Journal of the Optical Society of Korea
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    • 제18권1호
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    • pp.45-49
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    • 2014
  • Well-crystallized $BaSi_2O_5:Eu^{2+}$ phosphor films were synthesized by heat treatment of spin-coated BaO:Eu on $SiO_2$ glass. We investigated luminescence-structure properties of these phosphor films as a function of heat-treatment temperature. From x-ray diffraction patterns, our $BaSi_2O_5:Eu^{2+}$ phosphor films revealed that (111)- and (204)-crystal planes of $BaSi_2O_5$ crystal were dominantly increased with an increase of heat-treatment temperature. Photoluminescence intensities of $BaSi_2O_5:Eu^{2+}$ phosphor films were increased with amount of these crystal planes. It can be explained that $Eu^{2+}$ ions were stably occupied at specific crystal orientation of $BaSi_2O_5$ crystal, enhancing the luminescent intensities of $BaSi_2O_5:Eu^{2+}$ phosphor films. In addition, our $BaSi_2O_5:Eu^{2+}$ phosphor films had transmittance of 70% at 510 nm,.due to the dense morphology and specific crystallinity of $BaSi_2O_5:Eu^{2+}$ phosphor films.

부유대용융법에 의한 YMnO$_3$단결정 성장 (The growth YMnO$_3$ single crystals using a floating zone method)

  • 권달회;강승구;김응수;김유택;심광보
    • 한국결정성장학회지
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    • 제10권4호
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    • pp.279-285
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    • 2000
  • 부유대용융법에 의해 비휘발성 기억소자용 강유전성물질인 $YMnO_3$단결정을 육성하였다. 결정성장전 YMnO$_3$분말의 최적합성조건은 $1200^{\circ}C$에서 10시간, 최적원료봉 소결조건으로 $1500^{\circ}C$에서 10시간이었다. 초기 Seed가 없는 상태에서의 실험에서 성장된 단결정의 우선성장방위는 X-ray Laue분석을 통하여 [1010] 임을 알수 있었고 이 결정을 seed로 사용하여 c-축에 수직한 방향으로 $YMnO_3$단결정을 성장하였다 성장된 단결정은 직경 5mm, 길이 50 mm로 양질의 흑청색 결정이었다.

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데칸술폰이 삽입된 니켈 화합물의 층상 구조의 열적 성질 (Thermal behavior of the layered structure of decanesulfonate intercalated into the hydrated nickel compound)

  • 허영덕;전태현;박용준
    • 한국결정성장학회지
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    • 제9권6호
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    • pp.580-584
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    • 1999
  • 수화된 니켈에 데칸술폰이 층간 삽입된 화합물을 합성한 후 성질을 확인하였다. 화합물의 층상구조는 고온 X-선 회절 데이터로 확인하였다. 온도가 증가하면 층간 삽입된 니켈화합물의 구조가 변하는 것과 동시에 화합물의 층 거리가 24.7 $\AA$에서 30.5 $\AA$가지 증가하였다. X-선 회절 데이터와 데칸술폰의 크기로부터 니켈 층에 결합된 데칸술폰의 공간배열을 결정하였다. 이중 층 구조를 가진 데칸술폰의 분자 축은 니켈 층에 경사진 각도로 수직하게 배열되어있다.

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알킬술폰이 삽입된 층상구조의 염기성 초산구리의 합성과 구조 (The structure and synthesis of intercalation compounds between layered basic copper acetate and alkyl sulfonates)

  • 조영식;허영덕
    • 한국결정성장학회지
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    • 제8권3호
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    • pp.431-434
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    • 1998
  • 층상구조를 가진 염기성 초산구리, $Cu(OH)(CH_3COO){cdot}H_2O$를 합성하였다. 또한 음이온 치환반응을 통해서 alkyl sulfonate를 염기성 초산구리에 삽입시켰다. X-선 회절 데이터와 alkyl sulfonate의 분자의 크기를 비교하여 층간 삽입된 초산구리의 공간배열을 확인하였다.

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