• 한국초지조사료학회지
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• 제18권4호
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• pp.345-350
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• 1998

본 시험은 우리나라의 조사료자원 확대 방안을 모색하는 노력의 일환으로 대부분의 축산농가에서 조사료원으로 이용하고 있는 볏짚의 생육기별에 따른 사료가치를 구명하기 위하여 1998년 4월부터 동년 11월까지 우석대학교 부속 시험포장에서 수행하였으며 그 결과는 다음과 같다. 1. 볏짚의 생육기별 섬유소와 조회분 함량은 벼의 생육이 진행됨에 따라 증가하였으며, 조단백질과 조회분 함량은 감소하였다. NDF 함량은 큰 변화가 없었다. 황숙기 이후 hemicellulose 함량은 크게 감소하였고, cellulose 및 lignin 함량은 증가하였다. 2. 볏짚의 성숙기별 NDF의 반추위 분해율은 출수기때 가장 빨랐고, 유숙기부터는 비슷한 경향을 보였다. ADF의 반추위 분해율은 성숙기별에 따라 큰 변화를 나타내지 않았다. 따라서 볏짚의 성숙에 따른 화학적 조성분의 변화와 반추위 분해율을 종합해 볼 때, 출수기까지의 볏짚은 아직 목질화가 완성되지 않아 섬유질의 반추위 분해속도도 빨랐고, 그 이후의 볏짚은 사료적 가치가 급속히 낮아진다고 할 수 있다.

• Mycobiology
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• 제33권3호
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• pp.147-149
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• 2005

The investigation was undertaken to enhance the decomposition process by pre-treatment of rice stubble, having higher concentration of lignin. Air-dried rice stubble was treated with 1.8 liter of 1% NaOH and autoclaved. Six cellulolytic fungi, Trichoderma harzianum, Penicillium citrinum, Curvularia lunata, Aspergillus flavus and Alternaria alternata were grown in basal synthetic medium along with delignified rice-residue as carbon source for production of soluble crude protein. Though the loss of cellulose has been observed by all of them but having a considerable status in the presence of T. harzianum and T. harzianum yielded highest percentage of crude protein (27.99%) with biomass of 375 mg, whereas the lowest protein value (17.91%) was recorded in case of A. niger with biomass of 422 mg. Among the imperfect fungi, T. harzianum was the most potent. Effects of incubation period and nitrogen sources on soluble crude protein production by T. harzianum were also undertaken in this study. Fifth day of incubation period and potassium nitrate as nitrogen source among other nitrogen sources was found most appropriate for soluble crude protein production by the mentioned organism.

• Asian-Australasian Journal of Animal Sciences
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• 제4권2호
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• pp.177-182
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• 1991

Nutrient evaluation of sago fibre showed that the fibre has potential and could be utilized as feed for ruminants. However, as a source of nutrients, it has limitations arising from low intake, digestibility, crude protein and minerals content. The present study showed that the sago fibre is low in crude protein (3.3%) and high in neutral detergent fibre (72.5%) and acid detergent lignin (25.8%) contents. Treatment of sago fibre with urea increased the crude protein content from 3.3 to 16.7%. Both urea and sodium hydroxide treatment decreased the neutral detergent fibre level from 72.5 to 59 and 56.5%, respectively. Rumen degradation of sago fibre by nylon bag showed that both urea and sodium hydroxide treatments increased dry matter and organic matter disappearance of the fibre significantly. In vivo digestibility of 2% urea treated sago fibre was 47.5% and intake of the fibre was 1.57% of body weight of the lamb.

• 한국작물학회지
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• 제48권3호
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• pp.276-280
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• 2003

남부지역에 적응한 사초용 유채의 춘파 품종을 선발하기 위하여 3월 25일에 파종한 그 결과는 다음과 같다. 1. 다수성이며 조단백질 함량과 in vitro dry matter digestibility(IVDMD)가 높고 NDF, ADF, cellulose 및 lignin 함량이 낮아서 영양가가 우수한 춘파 용 사초 유채는 Velox 품종이었다. 2. Velox품종은 초장이 길고 경태가 두꺼웠으며 주경의 잎도 많았고 생체중 과 총건물수량이 가장 많았다. 3. 모든 형질에 대한 유전력은 대부분의 형질에서 높았으나 hemicellulose에서만이 낮게 평가되었다.

• 한국초지조사료학회지
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• 제25권3호
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• pp.169-176
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• 2005

본 연구에서는 국내에서 반추가축용 조사료 원으로 많이 사용하고 있는 화본과 수입조사료 6종(bermuda grass hay, klein grass hay, oat hay, orchard grass straw, tall fescue straw and timothy hay)에 대하여 최근 NRC의 nutrient requirements of daily cattle(7th revised edition, 2001)에서 권장하는 사료의 영양성분 분석방법에 따라 일반조성분과 함께 heat-stable $\alpha-amylase$ treated neutral detergent fiber (aNDF), acid detergent fiber (ADF), acid detergent lignin(ADL), neutral detergent insoluble crude protein(NDICP)과 acid detergent insoluble crude protein(ADICP) 등의 함량을 분석하고 영양성분간의 Pearson의 상관계수를 산출하였다. 조사료에 따라 다소 차이는 있으나 oat hay는 CP와 NDICP(p<0.05), CP와 ADICP(p<0.01)의 상관관계가 높게 나타났고, orchard grass straw는 CP와 NDICP의 상관관계가 높게 나타났다(p<0.05). Tall fescue straw도 CP와 NDICP의 상관관계가 높게 나타났으며(p<0.01), timothy hay는 CP와 ADICP의 상관관계가 높게 나타났다(p<0.05). Klein grass hay는 CF와 aNDF (p<0.01), oat hay는 CF와 ADF(p<0.05), orchard grass straw는 CF와 ADL(p<0.05), tall fescue straw는 CF와 aNDF(p<0.01), CF와 ADF(p<0.01), timothy hay는 CF와 aNDF(p<0.01), CF와ADF(p<0.05), CF와 ADL(p<0.01)에서 높은 상관관계를 나타내었다. 이렇게 영양성분 간에 상관관계가 높은 성분들 간에는 앞으로 조사료의 영양성분에 영향을 미치는 제 요인들을 고정하여 상관관계를 조사하고, 분석점수를 많이 하여 회귀식을 만들 수 있다면 수입조사료 평가에 실질적인 적용이 가능할 것으로 생각된다.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1098-1113
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• 2021

Maturation process of alfalfa (Medicago sativa L.) could be prevented by the reduction of lignin content in terms of conventional breeding or transgenic technology. Alfalfa could exhibit higher leaf/stem ratio, with a concern of yield loss. The objective of this study was to compare forage yield and nutritive value of low lignin alfalfa and two reference varieties subjecting to two harvest intervals and three seeding rates. The experimental design was a randomized complete block in a split-split plot arrangement with four replicates, where harvest intervals (28-day and 35-day) were assigned to whole plots, seeding rates were subplots, and varieties were sub-subplots. The weighted mean nutritive value was applied to two production years of 2016 and 2017. Hi-Gest 360 (low lignin alfalfa) provided similar yield potential and increased nutritive value compared to two reference varieties. Over a two-year production period, alfalfa harvested at every 28-day interval provided more economic returns than those at 35-day interval. For the seeding year and first production year, five cuts made by the 28-day interval produced more yield than four cuts by the 35-day interval. Due to limited rainfall in May 2017, a sharp drop of the first cutting overturned the advantage of the five-cut system. Shorter intervals between harvests generally increased crude protein (CP) concentrations. The differences of relative feed value (RFV) between two harvest intervals tended to be great during the first and second cuttings. Overall, harvest interval had a large effect on nutritive value and a more significant effect on alfalfa dry matter yield than variety selection. Seeding rate did not affect alfalfa yield and nutritive value.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.357-365
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• 2019

Objective: The experiment was conducted to evaluate the effects of four fungal pretreatments on the nutritional value of Camellia seed residues, and to evaluate the feeding value of pretreated Camellia seed residues for ruminants. Methods: Camellia seed residues were firstly fermented by four lignin degrading fungi, namely, Phanerochaete chrysosporium (P. chrysosporium)-30942, Trichoderma koningiopsis (T. koningiopsis)-2660, Trichoderma aspellum (T. aspellum)-2527, or T. aspellum-2627, under solid-state fermentation (SSF) conditions at six different incubation times. The nutritional value of each fermented Camellia seed residues was then analyzed. The fermentation profiles, organic matter degradability and metabolizable energy of each pre-treated Camellia seed residue were further evaluated using an in vitro rumen fermentation system. Results: After 5 days of fermentation, P. chrysosporium-30942 had higher degradation of lignin (20.51%), consumed less hemicellulose (4.02%), and the SSF efficiency reached 83.43%. T. koningiopsis-2660 degraded more lignin (21.54%) and consumed less cellulose (20.94%) and hemicellulose (2.51%), the SSF efficiency reached 127.93%. The maximum SSF efficiency was 58.18% for T. aspellum-2527 and 47.61% for T. aspellum-2627, appeared at 30 and 15 days respectively. All the fungal pretreatments significantly improved the crude protein content (p<0.05). The Camellia seed residues pretreated for 5 days were found to possess significantly increased organic matter degradability, volatile fatty acid production and metabolizable energy (p<0.05) after the treatment of either P. chrysosporium-30942, T. koningiopsis-2660 or T. aspellum-2527. The fungal pretreatments did not significantly change the rumen fermentation pattern of Camellia seed residues, with an unchanged ratio of acetate to propionate. Conclusion: The fungi showed excellent potential for the solid-state bioconversion of Camellia seed residues into digestible ruminant energy feed, and their shorter lignin degradation characteristics could reduce loss of the other available carbohydrates during SSF.

• Journal of the Korean Wood Science and Technology
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• 제51권1호
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• pp.38-48
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• 2023

Two important steps in utilizing technical kraft lignin (KL) from black liquor to synthesize lignin-phenol-formaldehyde (LPF) resin are its extraction via precipitation and fractionation. However, the effects of precipitation pH and acetone fractionation on the characteristics of hardwood KL have not been studied for LPF resins. Therefore, this paper reports the effects of the precipitation pH of black liquor and acetone fractionation on the characteristics of KL from mixed hardwood species for LPF resins. The precipitation was conducted at various pH levels from 3 to 9 of black liquor to obtain crude KL (C-KL), which was used in acetone fractionation to produce acetone-soluble KL (AS-KL) and acetone-insoluble KL (AI-KL). Precipitation at pH 3 and 9 produced the highest and lowest yields of C-KL, respectively. As expected, the C-KL infrared spectra were similar regardless of the precipitation pH levels. As the pH increased, the molecular weight of C-KL increased. However, the molecular weight of AS-KL and AI-KL after acetone fractionation increased to a maximum of 4,170 and 47,190 g/mol at pH 7, then decreased to 3,210 and 19,970 g/mol at pH 9, respectively. The smallest molecular weights of AS-KL and AI-KL were 3,210 and 15,480 g/mol and were found at pH 9 and 3, respectively. These results suggest that both AS-KL at pH 9 and AI-KL at pH 3 have good potential as starting lignins for synthesizing LPF resins that require cross-linking for polymerization.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1303-1308
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• 2003

Samples of eleven brown midrib (ICSU 96031, ICSU 93046, ICSU 96082, ICSU 96078, ICSU 96075, ICSU 95101, ICSU 96034, ICSU 96063, ICSU 45116, ICSA 93-3 and ICSA 3845 X 3816) and nine white midrib genotypes (ICSU 96050, ICSU 96030, ISU 95082, SSG 59-3, FSHI 93-1, FSHI 2219A X 3211, HC 171, ICSA 93-2 and ICSA 93-1) based on their phenotypic appearance were collected at 50 per cent flowering from the sorghum germplasm grown at Research farm of IGFRI, Jhansi. These genotypes were evaluated with respect to crude protein, fiber composition, in-sacco dry matter, OM, cell wall components disappearance/digestibility besides the fodder yield, total phenolic and availability index values. Brown midrib genotypes were lower (p<0.05) in NDF, ADF, cellulose and acid detergent lignin concentration than white midrib genotypes. Mean NDF, ADF, cellulose and lignin contents were 69.4, 42.1, 35.4 and 5.7% in brown mid rib vis-a vis 75.8, 47.5, 39.6 and 7.3% in white mid rib genotypes. Nonsignificant (p>0.05) differences were observed in dry matter, crude protein and organic matter contents between brown midrib and white midrib genotypes. Phenolic contents were significantly (p<0.05) lower in browm mid rib (0.2) than white mid rib (0.3%) sorghum. Brown midrib genotypes exhibited significantly (p<0.05) higher in-sacco DM, OM and CP disappearance than normal (white midrib) genotypes. The mean degradability of DM, OM and CP was 64.1, 62.6 and 79.6% in brown mid rib and 53.1, 54.0 and 76.6% in white mid rib genotypes, respectively. There were no significant (p>0.05) differences between genotypes in extent of fiber fraction degradability though in-sacco NDF and ADF degradability was more by 5 and 4 units, respectively in brown midrib genotypes vis-a-vis white midrib genotypes. Average fodder yield (green and dry g/plant) and availability index (%) values were significantly (p<0.05) higher for brown midrib (474.2, 129.8 and 80.4) genotypes than white midrib (375.0, 104.8 and 69.2) genotypes. Lignin contents had significant negative correlation with DM, OM, NDF and ADF degradability. The results of the study revealed that brown midrib genotypes are superior not only with regard to chemical entities and disappearance of DM and fiber fractions but also better in respect of fodder yield and availability index values. Thus, brown midrib sorghum strains may be useful in increasing digestibility, intake, feed efficiency and animal performance.

• 한국식품조리과학회지
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• 제7권3호
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• pp.69-76
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• 1991

6종류의 식용 버섯에 함유되어 있는 dietary fiber를 Southgate방법, NDF방법, FRI방법으로 측정한 결과는 다음과 같았다. 식용 버섯 중에 함유되어 있는 dietary fiber를 Southgate방법, NDF방법, FRI방법으로 측정한 결과는 각각 $20.08\pm1.45g$/100g dry weight, $20.24\pm1.85g$/100g dry weight, $27.5\pm2.7g$/100g dry weight이었고, 세가지 실험 방법간의 측정치 차이를 보면, 대체로 Southgate방법과 NDF방법과는 유의적인 차이가 없었던 반면, FRI방법과는 유의적인 차이가 크게 나타났다. 버섯중의 dietary fiber성분 비율은 Southgate방법에 의 한 water-soluble fiber성분이 1.7~3.1%, hemicellulose가 47.0~66.6%, cellulose가 28.4~57 7%, lignin 0.9~3.3%였고, NDF방법에 의해 측정한 hemicellulose는 61.8-79.1%, cellulose는 5.4~32.7%, lignin은 4.5~15.5% 였다. 일반적으로 버섯류의 dietary fiber성분은 hemicellulose가 가장 많았고 그 다음으로 cellulose가 많이 함유되어 있었다. Dietary fiber와 crude fiber의 함량비 (DF/CF)는 2~4배 정도로 dietary fiber가 높았다.