• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.783-788
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• 2005

To find antitumor components in liquid cultured cell-free broth and the hot water extract from the fruiting body of Hericium erinaceus, polysaccharides were purified and fractionated by DEAE-cellulose ion exchage column chromatography and Sepharose CL-6B gel filtration chromatography. Among various crude polysaccharides and purified polysaccharides the fractions showing immune-activity were determined by NO assay.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.52-55
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• 1998

During a survey of new antitumor substances from higher plants, we have found that the crude extract of Simaba cedron Planchon (Simaroubaceae) showed cytotoxic activity (IC$\sub$50/ 0.7 $\mu\textrm{g}$/mL) against P388 leukemia cells. Activity-guided chromatographic purification using P388 cells led to the isolation of five novel quassinoids, cedronolactones A-E (1-5) and nine known quassinoids, simalikalactone D (6), chaparrinon (7), chaparrin (8), glaucarubolone (9), glaucarubol (10), samaderine Z (11), guanepolide (12), ailanquassin A (13), and polyandrol (14). In this seminar, the structural elucidation of 1-5 and the cytotoxic activity of the isolated compounds are discussed.

• Bulletin of the Korean Chemical Society
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• v.25 no.3
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• pp.389-391
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• 2004

• Proceedings of the Ginseng society Conference
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• 1990.06a
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• pp.79-85
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• 1990

The aqueous extract of fresh ginseng (Panax ginseng C.A. Meyer) contains a macromolecular fraction that showed mitogenic and comitogenic activities in human peripheral blood Iymphocytes. Purification of the crude extract by size (ultrafiltration, Sephadex G-200) and charge (DEAE-cellulose. DEAE-Sepharose) yielded a semi-purified fraction (DS-3). This fraction contains at least three subgroups of anionic macromolecules with apparent molecular weight greater than 600 kilodaltons. It is a glycoprotein with a large amount of glucuronic acid. It acts as a mitogen in both T and B cells of human peripheral blood lymphocytes. It could also potentiate the mitogenic action of Concanavalin A in Iymphocyte T cells. Such potentiation is not due to increased binding of Concanavalin A to the cell surface. Its mitogenic and co-mitogenic effects do depend on the presence of extracellular Ca2+.

• Korean Journal of Pharmacognosy
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• v.14 no.3
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• pp.113-118
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• 1983

Studies were undertaken to develop for the anti-inflammatory analgesic activities of 'Insam-Tang' extract, which composed of four crude drugs; Ginseng Radix, Glycyrrhizae Radix, Atractylodis Rhizoma, and Zingiberis Rhizoma, was known to be applied in edema, convulsion, excremental blood, and cooling pain etc, in oriental herb remedies. It was exhibited significant anti-inflammatory effect on carrageenin inflammation, and preventive and therapeutic effects to chronic inflammation on Freund' complete adjuvant in rats, Especially, therapeutic effect of extract 500mg/kg was observed more effective than that of phenylbutazone 50mg/kg. Also, it was shown significant effect on leakage of dye into the peritoneal cavity and analgesic effect on squirm symptom induced by acetic acid in mice.

• Korean Journal of Pharmacognosy
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• v.13 no.2
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• pp.87-91
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• 1982

This study was undertaken to investigate the effect of 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang' on anti-inflammatory and antiulcerative actions, and motility of isolated intestine of mice. The results of this study were summarized as follows; Anti-inflammatory effect was showed markedly by the administration of water extract of 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang'. Isolated intestine of mice was relaxed with the treatment of 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang' and intestinal contraction induced by $BaCl_2$ and acetylcholine was also inhibited. The water extract of 'Jakyakgamcho-Tang' and parched 'Jakyakgamcho-Tang' were significantly inhibited Shay's ulceration in rats.

• Environmental Analysis Health and Toxicology
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• v.16 no.3
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• pp.127-131
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• 2001

The antihepatic and hypolipidemic activities and total lipid levels of water extract of Plantaginis Herba, used for respiratory and renal disorder in folk medicine, were evaluated in streptozotocin-induced diabetic rats. Diabetes was induced by a single injection of streptozotocin (50 mg/kg, i.ｐ.). Water extract of Plantaginis Herba recovered enzyme activities such as GOT, GPT, ALP and LDH increased by streptozotocin (STZ) treatment. This crude drug also suppressed the enhanced production of lipid in hyperglycemia. These results strongly suggest that Plantaginis Herba be a promising liver-protective and hypolipidemic agent.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2005.09a
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• pp.136-147
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• 2005

The inhibitor, a kind of ethyl acetate crude extract, was isolated from the old culture liquid of I.galbana. Through measured the culture density, the contents of chlorophyll a. polysaccharide and protein in the cells, the inhibitory effect of the inhibitor on the growth of cells was investigated. The results showed that the inhibitor obviously inhibited the cell growth of three microalgae.

• Journal of Marine Bioscience and Biotechnology
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• v.9 no.2
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• pp.49-57
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• 2017

We isolated marine bacterium, isolate DG-2 which produces the antibiotics against MRSA (methicillin-resistant Staphylococcus aureus). This isolate DG-2 was examined by its morphological, biochemical properties, and 16S rRNA sequencing analysis. And then, isolate DG-2 was identified to the genus Streptomyces. Therefore, this isolate was designated as Streptomyces sp. DG-2. Streptomyces sp. DG-2 grew relatively well at $25^{\circ}C$, pH 7.0, and NaCl 1.0%. For the pre-purification of the bioactive compounds, DG-2 was fermented in 30 L PPES-II medium, and the culture filtrates of DG-2 was extracted by ethyl acetate. The ethyl acetate extract of DG-2 showed the significant anti-MRSA and antibacterial activities.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.48-53
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• 1999

Isolation and quantitative determination of costunolide from Saussurea lappa Clarke (Compositae) has been conducted by using HPLC method. Costunolide in an acetone extract from the crude drug was separated on a RP-18 column using a $MeOH-H_20$ mixture (65:35) as an eluent and the average content is about $1.32{\sim1.42%.$ The content of costunolide in dried extract was decreased by about 24% in seven days. However it showed a slight decrease in solution. It is highly recommended that quantitative determination of costunolide from Saussureae Radix should be conducted as early as possible after solvent extraction.