• The Korean Journal of Food And Nutrition
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• v.21 no.2
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• pp.148-156
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• 2008

The purpose of this study was to analyze the changes in physicochemical characteristics and NDMA formation of Orostachys japonicus A. Berger according to harvest times. The results showed moisture, carbohydrate, crude fiber and crude protein contents of <$48.5{\pm}1.4%$, $38.2{\pm}1.2%$, $l5.8{\pm}0.4%$ and $13.2{\pm}0.6%$ respectively. The total mineral content was 4,817.1 mg/100 g, where Ca was highest($2,577.0{\pm}3.2$ mg/100 g), followed by K, Mg, Na, P and Fe. The composition amino acid of O. japonicus A. Berger showed high levels of glutamic acid and aspartic acid. And among the five free sugars detected, galactose and glucose were most abundant at $32.2{\pm}0.02$ mg/100 g and $25.3{\pm}0.1O$ mg/100 g, respectively. Upon comparing O. japonicus A. Berger extracts that came from plants harvested during $August{\sim}October$, total phenolic compound($2,703.l{\pm}5.2l{\sim}2,428.0{\pm}3.52$ mg/100 g) and flavonoid($634.2{\pm}2.33{\sim}1,324.6{\pm}1.87$ mg/100 g) contents were higher in the methanol extract than in the water extract. Also, within a reaction system, nitrite scavenging ability and NDMA inhibition were most effective at pH 2.5, and increased in proportion to the extract concentration. Finally, the methanol extract of O. japonicus A Berger harvested during $August{\sim}October$ had the highest nitrite scavenging and NDMA inhibition effects.

• The Korean Journal of Food And Nutrition
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• v.25 no.3
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• pp.570-578
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• 2012

Compound K(ginsenoside M1) is one of saponin metabolites and has many benefits for human health. This study was to investigate Compound K produced from ginseng crude saponin extract with commercial cellulolytic complex enzyme(cellulase, ${\beta}$-glucanase, and hemicellulase) obtained from Trichoderma reesei. The effect factors(temperature, pH, ginseng crude saponin extract and enzyme concentration, and reaction time) on production of Compound K from ginseng crude saponin extract were determined by one factor at a time method. The selected major factor variables were ginseng crude saponin extract of 2%(w/v), enzyme of 7%(v/v), reaction time of 48 hr. Based on the effect factors, response surface method was proceeded to optimize the enzymatic bioconversion conditions for the desirable Compound K production under the fixed condition of pH 5.0 and $50^{\circ}C$. The optimal reaction condition from RSM was ginseng crude saponin extract of 2.38%, enzyme of 6.06%, and reaction time of 64.04 hr. The expected concentration of Compound K produced from that reaction was 840.77 mg/100 g. Production of Compound K was 1,017.93 mg/100 g and 862.31 mg/100 g, by flask and bench-scale bioreactor($2.5{\ell}$) system, respectively.

• The Korean Journal of Food And Nutrition
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• v.30 no.1
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• pp.96-104
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• 2017

To obtain the immunomodulating polysaccharide from chaga mushroom (Inonotus obliquus sclerotia, IO), crude polysac- charide fractions (IO-M-CP and IO-CP, respectively) prepared from hot-water extract (IO-W) of I. obliquus by EtOH precipitation after MeOH reflux or not. After IO-W was re-dissolved in water followed by EtOH addition in the case without MeOH reflux, EtOH mixture was fractionated into EtOH-soluble (IO-E) and crude polysaccharide (IO-CP). In the meanwhile, MeOH-soluble fraction (IO-M) was separated from IO-W after MeOH reflux. The residue was dissolved in water and was added by EtOH, and then EtOH mixture was also fractionation into EtOH-soluble (IO-M-E) and crude polysaccharide (IO-M-CP). As a result of the macrophage stimulating activity of these fractions, IO-CP and IO-M-CP showed significantly increased cell proliferation and cytokines production than IO-W. Particularly, IO-M-CP promotes the production of IL-12 more than IO-CP. In the splenocytes proliferating activity and intestinal immune system modulating activity through Peyer's patch, both of 2 crude polysaccharide fractions were significantly promoted in cell proliferation and cytokines production than IO-W, and IO-M-CP was more potent than IO-CP in IL-2 production from splenocytes and GM-CSF production ($10{\mu}g/mL$) in Peyer's patch cells. In addition, immunomodulating polysaccharide fractions (IO-M-CP and IO-CP) prepared from IO-W by EtOH precipitation with or without EtOH reflux showed no significant difference in the chemical composition and component sugar. These results suggested that MeOH reflux might exclude low-molecular weight materials from IO-W and consequently increase the immunomodulating activity of IO-M-CP. Therefore, it was confirmed that immunomodulation of polysaccharide prepared from hot-water extract of chaga mushroom was enhanced by fractionation including MeOH reflux and EtOH precipitation.

• Microbiology and Biotechnology Letters
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• v.20 no.1
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• pp.1-5
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• 1992

Antibacterial activity of the water-soluble portion of Ulmus pzcmila L. extract against 10 bacterial species was studied by both cylinder plate dilution method and broth dilution test tube method. Inhibitory effect of the extract on the bacteria was also investigated by plotting bacterial survival at various concentration of the extract. The crude extract exhibited antibacterial activity against all of the tested bacterial species with exception of K pneurnoniae. The fractions of the extract prepared by CM Sephadex-C 50 ion exchange chromatography were also subjected to test the antibacterial activity, and the activity was studied after autoclaving for 20 minutes.

• Advances in Traditional Medicine
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• v.8 no.4
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• pp.395-399
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• 2008

Methanol and aqueous extract of leaves of Trema orientalis Linn. were subjected to the potential antioxidant and antibacterial activities. The pharmacological interest of this plant coupled with traditional use (antidiarrhoeal, antiseptic, analgesic etc) prompted to test for antioxidant and antibacterial activities. The antioxidant potential of the methanolic extract was determined on the basis of their scavenging activity of the stable 1,1-diphenyl-2-picryl hydrazyl free radical. $IC_{50}$ of the methanol extract of T. orientalis was $110.25\;{\mu}g/ml$ which indicated the strong antioxidant activity of the plant. However the aqueous extract showed mild antioxidant activity. In case of antibacterial activities test, the extract was subjected for its effectiveness against both Gram-positive and Gram-negative bacteria in agar diffusion method. The zones of inhibition produced by the crude methanol and aqueous extract against few sensitive strains were measured and compared with those of standard antibiotic Gentamycin. It is evident that both extracts are active against the bacteria at low concentrations. The obtained results provide a support for the use of this plant in traditional medicine and suggest its further advance investigation.

• Advances in Traditional Medicine
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• v.7 no.3
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• pp.286-289
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• 2007

Heracleum nepalense D Don. (Umbelliferae) is a small shrub having high glabrescent stem found in stream banks in Sikkim. Various medicinal properties which include antidiarrhoeal, antiseptic, anti-influenzal etc. have been attributed for this plant in the traditional system of medicine in Sikkim. In present investigation the methanol extract of roots of Heracleum nepalense was subjected for its effectiveness against both Gram-positive and Gram-negative bacteria causing diarrhoea. The roots extract was tested for its minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative organisms causing diarrhoea. Further, the zones of inhibition produced by the crude extract against few sensitive strains was measured and compared with those of standard antibiotic ciprofloxacin. It is evident that the methanol extract is very active against the bacteria causing diarrhoea at low concentrations. The antibacterial efficacy of the root extract was found to decrease in the following order against different tested bacterial strains like Shigella dysenteriae, Escherichia coli, Shigella boydii, Vibrio cholerae, Salmonella typhimurium.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.707-717
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• 2018

Leukocytes are reportedly the first line of the innate immune defense and essential for the control of common bacterial infections. Therefore, in this work, the antibacterial activity of crocodile leukocyte extract against Propionibacterium acnes was evaluated, and we also characterized the related activity of skin infection. The leukocyte extract showed the minimum inhibitory concentration to be $100{\mu}g/ml$ to P. acnes. SEM imaging demonstrated that the leukocyte extract adversely affected P. acnes cell permeability in a concentration-dependent manner. Furthermore, the crocodile leukocyte extract could significantly reduce proinflammatory markers and decrease inflammatory signs in infected mouse ears. The crude leukocyte extract was further purified using FPLC and RP-HPLC. The resulting fraction F5 was indicated as the anti-acne peptide-containing fraction. The molecular mass of the peptide contained in F5 was calculated to be 4,790.5 Da. N-Terminal sequencing revealed the amino acid sequence as GPEPVPAIYQ, which displays similarities to immunoglobulin A and leucine-rich repeat neuronal protein. This is the first reported amino acid sequence of a crocodile leukocyte extract that possesses anti-acne activity. To attempt to use it in a prototype cosmetic, an anti-acne gel containing crude crocodile leukocyte extract was formulated, resulting in seven gel formulations (G1, G2, G3, G4, G5, G6, and G7). The formulations G5, G6, and G7 exhibited 2-fold higher anti-acne activity than G1-G4. Investigation of accelerating stability studies of anti-acne gel formulations G5, G6, and G7 demonstrated that a low storage temperature ($4^{\circ}C$) is suitable for maintaining the physical properties and biological activity of the anti-acne gel products.

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.198.2-198.2
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• 2002

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.4
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• pp.353-366
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• 1992

This study was devised to purify the compound from tuna that have cytotoxic activities against various cancer cell lines and to observe its immunopotentiating activities. The cytotoxic compound was partially purified 277 fold, from petroleum ehter extract (crude extract) of tuna by silicic acid column chromatography (fraction D) and thin layer chromatography (Spot I). Cytotoxic activity was monitored using human colon cancer cell, HCT-48. The active compound (Spot I) was composed of seven materials which are fatty acids of four kinds ($C_{14:0},\;C_{16:0},\;C_{17:1},\;and\;C_{18:0}$) and unknown three fat materials. The active compound has cytotoxic activities against various cancer cell lines, that is, murine leukemic lymphocytes (L1210, P388) and human rectal (HRT-18) and colon cancer cells (HCT-48, HT-29). The patterns of size distribution of HCT-48 cells in the medium containing tuna extract were shifted to direction of the small size region. Also, the microscopic shape of HCT-48 cells were shrinked and distracted. The number of plaque forming cell and immunoglobin fraction of serum protein obtained from tuna-treated mice were increased, but natural killer cell activity was not affected.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.759-763
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• 1993

The antioxidant effects of aqueous green tea extract(AGTE) obtained from green tea and its combinations with several synergists on lard were investigated to equalize with that of crude catechin in green tea. The antioxidant effect of crude catechin was about 3-fold higher than that of AGTE. The suitable synergists for AGTE were ${\delta}-tocopherol$ and lecithin, while the tendency of their synergistic effects was different from each other. Addition of ${\delta}-tocopherol$ within 0.05% dosage to lard showed significant synergistic effect, and its synergistic effect decreased with the concentration of AGTE over 0.1% dosage. In combination of AGTE and lecithin, they gradually enhanced synergistic effect on lard with increasing the concentration. The combinations of AGTE, ${\delta}-tocopherol$ and lecithin showed intense synergistic effects and the optimum level of the each component was 0.1% AGTE, 0.05% ${\delta}-tocopherol$ and 1% lecithin. By adding the optimum level of three components to lard, the induction period was lenghtened approximately 8-fold than that of the control owing to their synergism. Comparing the antioxidant effect between the AGTE with synergists and crude catechin, the former was 60% and 20% higher than the latter at 0.1% and 1% dosage respectively.