• Journal of Ginseng Research
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• v.14 no.3
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• pp.442-446
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• 1990

As a part of studies on the quality control of crude drug-drink preparations, ginger components were identified by TLC and 6-gingerol was determined quantitatively by HPLC. Ginger components were identified by TLC with benzene/acetone (4:1, v/v, on silica gel plate by spraying a vanillinsulfuric acid reagent. 5-Gingerol contents were determined at 280 nm by HPLC on Lichro CART RP-18 column with acetonitrile/wate(38:62, v/v). Its transfer rate in the 3 types of crude drug extract drinks was 65.4-85.1% compared to the content in the ginger extract.

• Natural Product Sciences
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• v.11 no.4
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• pp.189-192
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• 2005

Anti-amnestic activities of Korean red ginseng (Ginseng Radix Rubra) and Crude drug-combined preparations (RGCDP-1, RGCDP-2, and RGCDP-3) were evaluated by the animal experiment. RGCDP-1 and RGCDP-2 were prepared based on Korean folk prescriptions, 'Chongmyongtang' and 'Guibitang', respectively, while RGCDP-3, by a combination of both. Among the three preparations, RGCDP-3 was found to show the most potent anti-amnestic activity as evaluated by the passive avoidance test with mice, indicating synergistic action by combined effect of RGCDP-1 and RGCDP-2.

• Korean Journal of Pharmacognosy
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• v.21 no.2
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• pp.126-129
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• 1990

Platelet anti-aggregating activities were evaluated with three combined crude drug preparations which have been used for 'eahyul'-one of the pathological concept in oriental medicine presumably concerning blood stasis or stagnant syndrome. The water extract of each combined preparation and each component plant was tested for their effects against ADP, arachidonic acid (AA) or collagen induced rat platelet aggregations. Mild inhibitory activities were observed with all the three tested preparations, Kaechibokryung-Hwan, Dangkqijakyak-San and Ohnkyung-Tang, and the component plants which are included in at least two of the above preparations.

• Journal of Pharmaceutical Investigation
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• v.17 no.1
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• pp.22-30
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• 1987

Evaluation method of crude drug preparations was studied in Saengmaek-san. Zig-zag TLC scanning profiles and high performance liquid chromatograms were obtained from Saengmaek-san and its each crude drug. A method using TLC densitometry and high performance liquid chromatography was established for the precise determination of ginsenoside $Rb_1$ in Saengmaek-san containing Ginseng Radix. Consequently, ginsenosicle $Rb_1$ content was 0.45-0.48 mg per g of Saengmaek-san. This method was found to be useful for the quality evaluation of oriental medicinal preparations.

• Proceedings of the Ginseng society Conference
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• 1993.09a
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• pp.206-214
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• 1993

Ginseng saponins have been known as main active principles and analyzed as the index components in ginseng and its products for quality control. But it is generally difficult to analyze the saponins in crude drug preparations. Saponins, Prosapogenins and sapogenins of crude drug preparation were identified by TLC and determined quantitatively by HPLC. $Prosapogemins-Rg_3\;-Rg_2\;and\;{\Delta}^{20}-prosapogenin$ were extracted with ethyl acetate from $50\%$ acetic acid hydrolyzates of saponin fractions and identified by TLC with lower phase of $CHCl_3/MeOH/H_2$ O\65:35:10. v/v)on silica gel plate, and quantified by HPLC on $Lichrosorb-NH_2$ column with $CH_3CN/H_2O(90:10,\;v/v).$ Sapogenins. panaxadiol and panaxatriol. were extracted with ethyl ether from $7\%-sulfuric$ acid hydrolyzates of saponin fractions and identified by TLC with chloroform/acetone(1 : 1 v/v) on silica gel plate. and quantified by HPLC on u - Bondapak $C^{18}$ column with $CH_3CN/MeOH/CHCl_3(83:10:7.\;v/v).$ These analyses of prosapogenins and sapogenins are more useful for quality control than those of saponins in crude drug preparations such as So - Shi - Ho - Tang(소시호탕), Sa - Kun - Ja - Tang(사군자탕), Yook - Kun - Ja - Tang(육군자탕), and In - Sam -Tang(인삼탕)

• Korean Journal of Pharmacognosy
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• v.30 no.2
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• pp.177-181
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• 1999

'Chung Kwei Won(淸快園)' is a Chinese patent medicine, which is used for stomachic disorder, acute and chronic indigestion, dispepsia, vomiting and diarrhea in Korea. This preparation consists of 16 kinds of powdered crude drugs. For the identification of individual crude drugs in such powdery mixtures, microscopic method may be used conviniently as it requires only a small amount of specimens. In this paper, the effectiveness of this method is exemplified by the identification of the ingredients in 'Chung Kwei Won' which contains 16 powder crude drug ingredients.

• The Journal of Korean Medicine
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• v.35 no.3
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• pp.135-154
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• 2014

Purpose: There is an issue in applying various principles introduced in established Korean medical classics to "Pharmaceuticals Approval, Notification and Review" of "herbal medicinal preparations" and "new drugs from natural products" that are used for western forms of medical treatment. Thus, an analysis of the origin, purpose, and application of established Korean medical classics in the Korean Pharmaceutical Affairs Act is essential. Methods: We collected data regarding the origin, purpose, and application of established Korean medical classics in the Korean Pharmaceutical Affairs Act, and classified them by periodical change and subjects. Results: Established Korean medical classics are applied as follows: 1) as criteria for Korean medicine distributors' sales of mixed herbal drugs (Pharmaceutical Affairs Act; since 1953), 2) as official compendiums for pharmacists' preparation of Korean medicine (Ministry of Health and Welfare's authoritative interpretation; from early 1970s to 1993), 3) as standards for oriental pharmacists' quality measurement of preparations (notification of the Ministry of Health and Welfare; since 1995), 4) as criteria for "Pharmaceuticals Approval, Notification and Review" of herbal medicinal preparations and crude drug preparations (notifications regarding drug approval process by the Ministry of Health and Welfare and the Ministry of Food and Drug Safety; since 1978), and 5) as standards for the quality of materials of health functional food (from 2004 to 2011). Conclusion: The application of Korean medical classics has been closely related with the change of the laws, regulations, and systems that are relevant to Korean medicine, and it seems to be more favorable for pharmacists than oriental pharmacists. Meanwhile, regulations that apply prescriptions that are recorded in Korean medical classics - dosage, indications, and preparation methods - as criteria for the approval of crude drug preparations for western medical treatment should be abolished.

• Korean Journal of Pharmacognosy
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• v.39 no.4
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• pp.316-323
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• 2008

In this study, we have investigated the HPLC analysis methods and quantitative analysis of standard compounds for quality standardization of a medicinal crude drug GCSB-5, a herbal formulation consisting of 6 medicinal plants (Acanthopanacis Cortex, Achyranthis Radix, Ledebouriellae Radix, Cibotii Rhizoma, Glycine Semen, Eucommiae Cortex) which are used in traditional medicine to treat various bone disorders. HPLC analysis methods of acanthoside D(Acanthopanacis Cortex), 20-hydroxyecdysone(Achyranthis Radix) which were known standard compounds among 6 medicinal plants were developed on crude material and product. And validation of HPLC analysis methods were conformed for verification of HPLC methods by check to specificity, linearity, intra-day precision, inter-day precision and accuracy following ICH guideline. Content of acanthoside D and 20-hydroxyecdysone on raw material of GCSB-5 were decided at 0.577-0.578 mg/g and 0.311-0.312 mg/g. And we confirmed that content of acanthoside D and 20-hydroxyecdysone on GCSB-5 preparation were 0.302-0.303 mg/capsule and 0.113-0.115 mg/capsule.

• The Korean Journal of Pharmacology
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• v.7 no.1
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• pp.29-35
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• 1971

The effect of Capricornidis Cornu (C.C) on the amount of total cholesterol, triglycerides, and phospholipid in serum from rabbit were observed. The preparations were extracted by methanol, and extracted samples were oraly adminstrated to the rabbits fasted for three days. After three days starvation, the amounts of total cholesterol and triglycerides in serum of the rabbit were markedly increased, while phospholipid was slightly decreased. The amount of total cholesterol and triglycerides were gradually decreased by adminstration of the extracted samples and were returned to the control leveles after 15 days. However, the amount of phospholipid was increased to higher than the control levels by adminstraion of the drug. According to the above results, it would be concluded that the extract of C.C. and crude drug preparation containing C.C. influences for the recovery of the increased amounts of total cholesterol and triglycerides by abnormal metabolism to the normal on rabbit.

• Korean Journal of Oriental Medicine
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• v.2 no.1
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• pp.506-513
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• 1996

The odor theory(氣味論) has been defined as the Nature(氣) and the Taste(味) of traditional herbal medicine to find the logic in treatment of various diseases by them. There is a strong possibility these Natures of the drug can be categorized according to yin(陰) and yang(陽). To understand the Hot and the Cold Natures of traditional herbal drugs in scientific approaches, changes in skin, rectal temperature of rats have been measured at 0, 30, 60, 90 min after a 5, 10, 20g/Kg oral administration each of Coptidis Rizoma(Ranunculaceae, Coptis chinesis, $F_{RANCH}$), Aconiti Lateralis Preparata Radix(Ranunculaceae, Aconitum carmichaeli $D_{EBX}$). From this study, we obtained as follows. 1. We can categorize the four Natures of drug according to yin(陰) - Cool and Cold Natures and yang(陽) - Warm and Hot Natures. 2. The relation between the four Natures of drug and the changes of body temperature can be studied as the considertion of the dosage and the preparation of crude medicines. 3. Herbs containing toxicant should be studied carefully without the side effects.