• Journal of Korean Society of Forest Science
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• v.101 no.2
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• pp.291-296
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• 2012

Triploids are a useful tool for biomass production and molecular breeding of trees with a long life span. Triploids of the poplar 'Hyunsasi' (Populus alba ${\times}$ P. glandulosa) have been developed by crossing between female diploids and a male tetraploid. The tetraploid was developed around the 1970s at Korea Forest Research Institute by colchicine-induced chromosome doubling. Seedlings of the $F_1$ generation were analyzed using flow cytometry to verify their ploidy status. The mean relative fluorescence index of 3 F1 poplars, labeled as Line- 1, Line-17, Line-18, were approximately 1.5 times higher than those of diploid poplars, and the results clearly indicated that they were triploids. The phenotype of the F1 poplars included larger leaves and thicker stem than diploids, and abnormal leaf morphology, especially in the triploid 'Line-18'. Three triploid lines developed roots more slowly and had less roots than diploid. However, 3 poplar cytotypes (2x, Line-1, Line-17) rooted within 10 days on MS medium. In contrast, compared with the 3 cytotypes, the Line-18 showed about 80% and 70% in the rooting rate and the number of roots. The triploid poplars could be directly utilized for biomass production and with their sterility, they could serve as basic material for genetic transformation. In addition, flow cytometric analysis proved to be an effective and reliable method for screening forest trees for their ploidy level.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.6
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• pp.532-538
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• 1990

This experiment was carried out to establish the efficiency of crop breeding on comparison of combination methods of single, 3-way, and double crosses and combination order of 4 winter wheat which were different in origin, source, and plant types, On comparison of 4 crossing modes, there appeared the earliest heading and the highest grain yield in double cross, and decreased in 3-way. single crosses. and parents in order, There showed the significant mean squares of GCA and SCA in 4${\times}$4 diallel analysis for grain yield and yield components. Grumil and Bezostaya 1 exhibited highest GCA effect of grain yield which appeared the actual highest grain yield. There appeared the highest SCA-effect in F$_1$ (Eunpamil/Bezostaya 1) showing 4.22. Of the 3 double crosses there exhibited the highest grain yield in F$_1$ (Grumil/Eunpamil/ /Lanota/Bezostaya 1). Two single crosses for this double cross ---F$_1$ (Grumil/Eunpamil) and F$_1$ (Lancota/Bezostaya 1) --- do not revealed directly for this yield, but combined each other by chromosome switch as combination of F$_1$ (Grumil/Lancota), F$_1$(Grumil/Bezostaya 1), F$_1$(Eunpamil/Lancota) and F$_1$(Eunpamil/Bezostaya 1) which appeared the higher grain yields and SCA-effects. Of the six 3-way crosses. F$_1$ (Lancota/Bezostaya 1/ /Eunpamil) expressed the highest grain yield. Its combinations were F$_1$ (Lancota/Eunpamil) and F$_1$ (Bezostaya 1/Eunpamil) combined by chromosome switch, which its grain yield and SCA-effect were higher.

• Journal of Mushroom
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• v.16 no.3
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• pp.198-202
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• 2018

'Yong-A' was bred by Jeollanamdo Agricultural Research and Extension Services in 2015. It was primarily selected through monospore crossing between JNM21008 and JNM21013 in 2009. The strain is named as JNM-Mi-194 and 'Yong-A', showing major characteristics with good production yield. The cultivation characteristics, yield potential, and fruiting body characteristics of 'Yong-A' are as follows; The wrinkle of the fruiting body was of branching type its shape was wave type. MCM, YM, Malt, and PDA media were suitable for growth of this cultivar. The number of effective stipes was 13 ea/0.9 kg. The minor axis of pileus was 5 cm and the major axis was 9 cm. The yield was 291 g per plastic bag (0.9 kg). Yong-A required 60 days for mycelial running at $20^{\circ}C$. The growth and primordial period required 24 days, which was shorter than that of JNM-Mi-21002 (the conventional cultivar). Somatic incompatibility was observed between parental lines and Yong-A. Analysis of genetic diversity in the new variety 'Yong-A' showed a different profile compared to that of the parent strain when Random Amplified Polymorphic DNA primers were used.

• Journal of Mushroom
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• v.16 no.3
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• pp.192-197
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• 2018

A new blackish-brown variety of Flammulina velutipes, 'Yeoreumhyang2ho,' which was bred by crossing two monokaryons isolated from the 'Heukhyang' and 'Garlmoe' varieties, showed adaptability to high temperature. During bottle cultivation, the temperature was maintained at $16^{\circ}C$ and the variety displayed good productivity compared to the control ('Heukhyang'), without necessitating low temperature ($4^{\circ}C$) treatment. The period necessary for mycelial and fruit body growth, and primordia formation was 24 days, 8 days, and 8 days, respectively. The total cultivation period was 40 days, which was 3 days shorter than that of the control. Particularly, primordia formation took 2 days less than the control and showed initial uniformity. The yield of 'Yeoreumhyang2ho' was 154 g per 850 ml bottle, which was 8% higher than the control yield. The ${\beta}-glucan$ content was 67.9 g per 100 g dry weight, which is approximately 1.1-2.2 times the amount of ${\beta}-glucan$ in control, the golden and white mushrooms that are available in the market.

• Journal of Mushroom
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• v.11 no.2
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• pp.77-81
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• 2013

To develop a new cultivar of king oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strain ASI 2824-21 derived from ASI 2824(Keunneutari No. 2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-53(G09-21-10 x ASI 2844-9) was shown the best cultural characteristics, selected to be a new cultivar and designated as 'Seolsong'. The 'Seolsong' was distinctly formed incompatibility line in the confrontation growth of parental strains Keunneutari No. 2, Aeryni 3 and ASI 2844. Analysis of the genetic characteristics of the new cultivar 'Seolsong' showed a different DNA profile as that of the control strains, Keunneutari No. 2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primer URP4 was used. The optimum temperature and pH arrange for mycelial growth were $25{\sim}30^{\circ}C$ and pH 5~8, respectively. This new cultivar 'Seolsong' of fruiting body production per bottle was about $131.4{{\pm}}43.1$ g which is about 102% quantity compared to that of other cultivar Keunneutari No. 2. And also the stipe is thick and long, but the number of available stipe is few. Particularly, it was tolerant of high moisture above 90% during the growth period after primodia formation. We therefore expect that this new strain will save of labor and cost of cultivation by without culling work.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.464-469
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• 2015

This study was carried out to find relationships of genetic distance and heterosis expression degree in the developed CNU waxy corn lines. The used material were developed in Corn Breeding Laboratory, Coll. of Agri. & Life Sci., CNU. Total 10 primers used for SSR maker analysis. In genetic distance, the used lines divided into four groups; A group has two lines, B group has one line, C group has ten lines and D group has eleven lines, respectively. Among used lines, CNU427 and CNU588 were very closed as a 74, while CNU451 and CNU429 were remote as a 40 inbred coefficient, respectively. While heterosis degree were very variable not only hybrids but also cross parents. Average heterosis of most cross-parent was high in plant height, ear height, flowering day and ear length. Especially, CNU H09-23 hybrid was high as 79.4% than other hybrids. Here, we gained the fact has closely relationship between genetic distance and heterosis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.4
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• pp.436-441
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• 2015

'Superhongmi' is a new rice variety with reddish brown color developed from a crossing a high cyanidin 3-glucoside (C3G) content beeeds including Heugjinju and Suwon 425 and a big grain size breed such as Daeribbyeo. This variety heads on Sep. 5 and has 86.7 cm culm length. The fertility ratio of Superhongmi was about 81.7% and weight of 1,000-grain was about 26.8 g. The 1,000 grain weight of Superhongmi was heavier than that of Superjami. The total essential amino acid content of Superhongmi was lower than those of others. However, Superhongmi was high in tyrosine and arginine. Total polyphenol content of Superhongmi was 2 times higher than that of Heugjinju and flavonoid content was 8 times higher than that of Dongjinbyeo. In addition, hydroxy radical scavenging activity of Superhongmi was significantly higher than that of Dongjinbyeo. However, significant differences were not found in the newly breed colored rice. These results suggest that Superhongmi variety has very high value as a source of various functional food as well as stable food.

• Horticultural Science & Technology
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• v.28 no.3
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• pp.519-521
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• 2010

An Asiatic lily cultivar 'Asian Flame' was bred in 2007 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) of Korea. The crossing was conducted between female parent A94-34 ('Montreux' ${\times}$ 'Anabelle') and male parent 'Fire' by stigmatic pollination at Suwon in 1996. First selection was done and tentatively named as A98-98 in 1998. After in vitro multiplication and bulb production of A98-98 line, growth and flowering characteristic tests has been conducted from 2005 to 2007. The evaluation of characteristics and preference were surveyed at a lily flower show of NIHHS in 2007 in Taean. The new cultivar 'Asian Flame' flowers in middle of June and grows to an average of 122.7 cm in height. Flowers bloom upward-facing, red (RHS 45B) petals. Year-round flowering is possible by storage of the bulb under $-1.5^{\circ}C$ conditions. For forcing culture, it is necessary to add calcium to the fertilizer or remove side scales of large bulb to prevent leaf scorch.

• Horticultural Science & Technology
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• v.31 no.1
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• pp.128-132
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• 2013

A new pink gerbera (Gerbera hybrida Hort.) cultivar 'Party Time' was developed from a cross between 'Hope' and 'Sunnyeo', followed by seedling and line selections at the Flower Research Institute of Gyeongnam Agricultural Research and Extension Services (GARES) in 2007. Detailed characteristics of the new cultivar were evaluated from 2005 to 2007. 'Party Time' has semi-double type large flowers in a good harmony with pink (RHS, 65-C) ray floret and brown center. It has a good, stable flower shape and strong peduncles, and its vase life was 12.7 days. The average flower yield of 'Party Time' was about 47.3 stems per plant a year in greenhouse yield trails carried out 2005 to 2007. 'Party Time' (Grant No. 2691) was registered to the Korea Seed and Variety Service (KSVS) for commercialization in 2009.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.149-154
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• 2015

A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.